Cotton fiber is one of the main raw materials for the textile industry.In recent years,many cotton fiber quality QTL have been identified,but few were applied in breeding.In this study,a genome wide association study(...Cotton fiber is one of the main raw materials for the textile industry.In recent years,many cotton fiber quality QTL have been identified,but few were applied in breeding.In this study,a genome wide association study(GWAS)of fiber-quality traits in 265 upland cotton breeding intermediate lines(GhBreeding),combined with genome-wide selective sweep analysis(GSSA)and genomic selection(GS),revealed 25 QTL.Most of these QTL were ignored by only using GWAS.The CRISPR/Cas9 mutants of GhMYB_D13 had shorter fiber,which indicates the credibility of QTL to a certain extent.Then these QTL were verified in other cotton natural populations,5 stable QTL were found having broad potential for application in breeding.Additionally,among these 5 stable QTL,superior genotypes of 4 showed an enrichment in most improved new varieties widely cultivated currently.These findings provide insights for how to identify more QTL through combined multiple genomic analysis to apply in breeding.展开更多
Background:Egg production is economically important in the meat-type chicken industry.To better understand the molecular genetic mechanism of egg production in meat-type chicken,genetic parameter estimation,genome-wid...Background:Egg production is economically important in the meat-type chicken industry.To better understand the molecular genetic mechanism of egg production in meat-type chicken,genetic parameter estimation,genome-wide association analyses combined with meta-analyses,Bayesian analyses,and selective sweep analyses were performed to screen single nucleotide polymorphisms(SNPs)and other genetic loci that were significantly associated with egg number traits in 11,279 chickens from seven material lines.Results:Yellow-feathered meat-type chickens laid 115 eggs at 43 weeks of age and white-feathered chickens laid 143 eggs at 60 weeks of age,with heritability ranging from 0.034–0.258.Based on meta-analyses and selective sweep analyses,one region(10.81–13.05 Mb)on chromosome Z was associated with egg number in all lines.Further analyses using the W2 line was also associated with the same region,and 29 SNPs were identified that significantly affected estimation of breeding value of egg numbers.The 29 SNPs were identified as having a significant effect on the egg number EBV in 3194 birds in line W2.There are 36 genes in the region,with glial cell derived neurotrophic factor,DAB adaptor protein 2,protein kinase AMP-activated catalytic subunit alpha 1,NAD kinase 2,mitochondrial,WD repeat domain 70,leukemia inhibitory factor receptor alpha,complement C6,and complement C7 identified as being potentially affecting to egg number.In addition,three SNPs(rs318154184,rs13769886,and rs313325646)associated with egg number were located on or near the prolactin receptor gene.Conclusion:Our study used genomic information from different chicken lines and populations to identify a genomic region(spanning 2.24 Mb)associated with egg number.Nine genes and 29 SNPs were identified as the most likely candidate genes and variations for egg production.These results contribute to the identification of candidate genes and variants for egg traits in poultry.展开更多
With the prevailing power scenario, every watt-second of electrical energy has its own merit in satisfying the consumer demand. At the state of such a stringent energy demanding era, failure of a power generation equi...With the prevailing power scenario, every watt-second of electrical energy has its own merit in satisfying the consumer demand. At the state of such a stringent energy demanding era, failure of a power generation equipment compounds the energy constraints which will not only result in a huge loss of generation but also have an impact on capital revenue. The unexpected failure of generator step-up （GSU） transformer is espe- cially a major disturbance in the power system operation and leads to unscheduled outages with power delivery problems. The time lag in bringing back the equipment in service after rectification or replacement may increase the criticality as the process involves mobilization of spares and maintenance professionals. Hot atmosphere existing in the vicinity of thermal power stations running round-the- clock with more than 100% plant load factor （PLF） increases the thermal stress of the electrical insulation which leads to premature failure of windings, bushings, core laminations, etc. The healthy state of the GSU transformer has to be ensured to minimize the loss of power generation. As the predication related to failure of a GSU transformer is associated with some uncertainties, a fuzzy approach is employed in this paper along with actual field data and case studies for the well-being analysis of GSU transformer.展开更多
A 52 MHz Radio Frequency Quadrupole （RFQ） linear accelerator （linac） is designed to serve as an initial structure for the SSC-Linac system （injector into Separated Sector Cyclotron）.The designed injection and ou...A 52 MHz Radio Frequency Quadrupole （RFQ） linear accelerator （linac） is designed to serve as an initial structure for the SSC-Linac system （injector into Separated Sector Cyclotron）.The designed injection and output energy are 3.5 keV/u and 143 keV/u,respectively.The beam dynamics in this RFQ have been studied using a three-dimensional Particle-In-Cell （PIC） code BEAMPATH.Simulation results show that this RFQ structure is characterized by stable values of beam transmission effciency （at least 95%） for both zerocurrent mode and the space charge dominated regime.The beam accelerated in the RFQ has good quality in both transverse and longitudinal directions,and could easily be accepted by Drift Tube Linac （DTL）.The effect of the vane error and that of the space charge on the beam parameters have been studied as well to define the engineering tolerance for RFQ vane machining and alignment.展开更多
基金supported by National Key Research and Development Program of China(2022YFF1001400)the National Natural Science Foundation of China(31830062 and 32172071)+1 种基金Innovation and Application of Superior Crop Germplasm Resources of Shihezi(2021NY01)Breeding of New Cotton Varieties and Application of Transgenic Breeding Technology(2022NY01)。
文摘Cotton fiber is one of the main raw materials for the textile industry.In recent years,many cotton fiber quality QTL have been identified,but few were applied in breeding.In this study,a genome wide association study(GWAS)of fiber-quality traits in 265 upland cotton breeding intermediate lines(GhBreeding),combined with genome-wide selective sweep analysis(GSSA)and genomic selection(GS),revealed 25 QTL.Most of these QTL were ignored by only using GWAS.The CRISPR/Cas9 mutants of GhMYB_D13 had shorter fiber,which indicates the credibility of QTL to a certain extent.Then these QTL were verified in other cotton natural populations,5 stable QTL were found having broad potential for application in breeding.Additionally,among these 5 stable QTL,superior genotypes of 4 showed an enrichment in most improved new varieties widely cultivated currently.These findings provide insights for how to identify more QTL through combined multiple genomic analysis to apply in breeding.
基金the Guangdong Provincial Key Research and Development Program(2020B02022002)the Shandong Agricultural Seed Improvement Project(2020LZGC013)the Technological Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202005)。
文摘Background:Egg production is economically important in the meat-type chicken industry.To better understand the molecular genetic mechanism of egg production in meat-type chicken,genetic parameter estimation,genome-wide association analyses combined with meta-analyses,Bayesian analyses,and selective sweep analyses were performed to screen single nucleotide polymorphisms(SNPs)and other genetic loci that were significantly associated with egg number traits in 11,279 chickens from seven material lines.Results:Yellow-feathered meat-type chickens laid 115 eggs at 43 weeks of age and white-feathered chickens laid 143 eggs at 60 weeks of age,with heritability ranging from 0.034–0.258.Based on meta-analyses and selective sweep analyses,one region(10.81–13.05 Mb)on chromosome Z was associated with egg number in all lines.Further analyses using the W2 line was also associated with the same region,and 29 SNPs were identified that significantly affected estimation of breeding value of egg numbers.The 29 SNPs were identified as having a significant effect on the egg number EBV in 3194 birds in line W2.There are 36 genes in the region,with glial cell derived neurotrophic factor,DAB adaptor protein 2,protein kinase AMP-activated catalytic subunit alpha 1,NAD kinase 2,mitochondrial,WD repeat domain 70,leukemia inhibitory factor receptor alpha,complement C6,and complement C7 identified as being potentially affecting to egg number.In addition,three SNPs(rs318154184,rs13769886,and rs313325646)associated with egg number were located on or near the prolactin receptor gene.Conclusion:Our study used genomic information from different chicken lines and populations to identify a genomic region(spanning 2.24 Mb)associated with egg number.Nine genes and 29 SNPs were identified as the most likely candidate genes and variations for egg production.These results contribute to the identification of candidate genes and variants for egg traits in poultry.
文摘With the prevailing power scenario, every watt-second of electrical energy has its own merit in satisfying the consumer demand. At the state of such a stringent energy demanding era, failure of a power generation equipment compounds the energy constraints which will not only result in a huge loss of generation but also have an impact on capital revenue. The unexpected failure of generator step-up （GSU） transformer is espe- cially a major disturbance in the power system operation and leads to unscheduled outages with power delivery problems. The time lag in bringing back the equipment in service after rectification or replacement may increase the criticality as the process involves mobilization of spares and maintenance professionals. Hot atmosphere existing in the vicinity of thermal power stations running round-the- clock with more than 100% plant load factor （PLF） increases the thermal stress of the electrical insulation which leads to premature failure of windings, bushings, core laminations, etc. The healthy state of the GSU transformer has to be ensured to minimize the loss of power generation. As the predication related to failure of a GSU transformer is associated with some uncertainties, a fuzzy approach is employed in this paper along with actual field data and case studies for the well-being analysis of GSU transformer.
基金Supported by National Natural Science Foundation of China (10635090)
文摘A 52 MHz Radio Frequency Quadrupole （RFQ） linear accelerator （linac） is designed to serve as an initial structure for the SSC-Linac system （injector into Separated Sector Cyclotron）.The designed injection and output energy are 3.5 keV/u and 143 keV/u,respectively.The beam dynamics in this RFQ have been studied using a three-dimensional Particle-In-Cell （PIC） code BEAMPATH.Simulation results show that this RFQ structure is characterized by stable values of beam transmission effciency （at least 95%） for both zerocurrent mode and the space charge dominated regime.The beam accelerated in the RFQ has good quality in both transverse and longitudinal directions,and could easily be accepted by Drift Tube Linac （DTL）.The effect of the vane error and that of the space charge on the beam parameters have been studied as well to define the engineering tolerance for RFQ vane machining and alignment.
