利用生物信息学方法对番茄SUTs基因家族成员基因及蛋白质序列进行生物信息学分析,并对基因的表达特性进行研究。番茄SlSUTs基因家族共有3个成员,蛋白质的分子质量在54.15~64.99 k D之间;3个基因均匀分布在4号、5号和11号染色体上;基因...利用生物信息学方法对番茄SUTs基因家族成员基因及蛋白质序列进行生物信息学分析,并对基因的表达特性进行研究。番茄SlSUTs基因家族共有3个成员,蛋白质的分子质量在54.15~64.99 k D之间;3个基因均匀分布在4号、5号和11号染色体上;基因的内含子和外显子结构模式有3种,差异显著。番茄SlSUTs和拟南芥AtSUTs氨基酸都具有2种基序存在形式,且相同基序形式的基序位置存在关系一致。番茄和拟南芥SUTs蛋白的二级结构都有4种,其中α螺旋占比最多,β折叠占比最少,蛋白具有11或12个跨膜结构域,亚细胞定位都在细胞膜上。植物SUTs基因的进化关系遵循物种种属的亲缘关系,番茄SlSUTs基因与葡萄的进化关系更相近。番茄SlSUTs基因在不同组织和果实不同发育时期的表达都不相同,说明SlSUTs基因的表达具有一定的组织特异性。展开更多
Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield...Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield remains unclear.In this study,we identified five rice SUT genes(OsSUT1-OsSUT5)and examined their molecular characteristics and biological functions.OsSUT1,OsSUT2,and OsSUT4 were predominantly expressed in stems,while OsSUT3 and OsSUT5 showed higher expression in panicles.OsSUT1 and OsSUT4 are located on the plasma membrane,whereas OsSUT2,OsSUT3,and OsSUT5 are localized to the tonoplast.展开更多
文摘利用生物信息学方法对番茄SUTs基因家族成员基因及蛋白质序列进行生物信息学分析,并对基因的表达特性进行研究。番茄SlSUTs基因家族共有3个成员,蛋白质的分子质量在54.15~64.99 k D之间;3个基因均匀分布在4号、5号和11号染色体上;基因的内含子和外显子结构模式有3种,差异显著。番茄SlSUTs和拟南芥AtSUTs氨基酸都具有2种基序存在形式,且相同基序形式的基序位置存在关系一致。番茄和拟南芥SUTs蛋白的二级结构都有4种,其中α螺旋占比最多,β折叠占比最少,蛋白具有11或12个跨膜结构域,亚细胞定位都在细胞膜上。植物SUTs基因的进化关系遵循物种种属的亲缘关系,番茄SlSUTs基因与葡萄的进化关系更相近。番茄SlSUTs基因在不同组织和果实不同发育时期的表达都不相同,说明SlSUTs基因的表达具有一定的组织特异性。
基金supported by the National Key Research and Development Program of China(Grant No.2023YFF1000500)the National Natural Science Foundation of China(Grant Nos.32372099,32172080,and 32188102)the Innovation Program of the Chinese Academy of Agricultural Sciences,China(Grant No.CAAS-CSCB-202402).
文摘Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield remains unclear.In this study,we identified five rice SUT genes(OsSUT1-OsSUT5)and examined their molecular characteristics and biological functions.OsSUT1,OsSUT2,and OsSUT4 were predominantly expressed in stems,while OsSUT3 and OsSUT5 showed higher expression in panicles.OsSUT1 and OsSUT4 are located on the plasma membrane,whereas OsSUT2,OsSUT3,and OsSUT5 are localized to the tonoplast.