Objective:To observe the sustained expression of transient outward potassium channel related proteins at the end of the treatment and 30 days after the end of the treatment in rats,and to explore the sustained curativ...Objective:To observe the sustained expression of transient outward potassium channel related proteins at the end of the treatment and 30 days after the end of the treatment in rats,and to explore the sustained curative effect and mechanism of acupuncture combined with Xijingtongmai decoction in rats with myocardial infarction.Methods:Twenty of 130 male SD rats were random extracted as the control group,and the rest were used to establish myocardial infarction by fed with high-fat diet and then injected with isoproterenol.According to ECG,80 rats were successfully established.Then they were randomly divided into model group,acupuncture combined with Chinese medicine group,acupuncture group and Western medicine group.The content of bFGF protein was measured by ELISA.The protein contents of Kv1.4,Kv4.2,Kv4.3 and KChIP2 were measured by Western blot.Results:At the end of treatment,compared with the model group,the protein contents of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF in each treatment group increased,and the increase was most significant in the acupuncture combined with Chinese medicine group(P<0.05).At the end of treatment,compared with the model group,the protein contents of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF in each treatment group increased,and the increase was most significant in the acupuncture combined with Chinese medicine group(P<0.05).Compared with the treatment group at the end of treatment,the expression of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF protein in each treatment group 30 days after the end of treatment decreased slightly(P<0.05),but still higher than that of the model group at this time(P<0.05).The combination of acupuncture and Chinese medicine group decreased the least of them(P<0.05).Conclusion:The results showed that acupuncture combined with xijingtongmai decoction had a sustained good effect.Its sustained action mechanism may be achieved by continuously increasing the protein content of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF through transient outward potassium channel.展开更多
Poly (D,L-lactide-co-glycolide) (PLGA) is a biodegradable and biocompatible polymer material for drug deliver system. The aim of this study is to synthesize drug-loaded
Effective utilization of sustainable energies necessitates the development of electrochemical energy conversion and storage devices,where the catalysts and ion-conducting membranes are two core components.Anion exchan...Effective utilization of sustainable energies necessitates the development of electrochemical energy conversion and storage devices,where the catalysts and ion-conducting membranes are two core components.Anion exchange membrane water electrolysis(AEM-WE)has been widely regarded as the next-generation technology for green hydrogen production because it provides a feasible route to achieve non-precious-metal catalyzed green energy conversion[1].Currently,the design of high-performance catalysts for the anodic oxygen evolution reaction(OER)and cathodic hydrogen evolution reaction(HER)has attracted wide attention and made impressive achievements[2],but the development of the indispensable AEM is relatively less mature[3].Therefore,designing AEMs with high conductivity,long-term stability,and mechanical strength represents one of the key challenges to realizing effective and economical water electrolysis with AEM-WE.展开更多
基金Key project of Liaoning provincial science and technology foundation(No.20180530079)。
文摘Objective:To observe the sustained expression of transient outward potassium channel related proteins at the end of the treatment and 30 days after the end of the treatment in rats,and to explore the sustained curative effect and mechanism of acupuncture combined with Xijingtongmai decoction in rats with myocardial infarction.Methods:Twenty of 130 male SD rats were random extracted as the control group,and the rest were used to establish myocardial infarction by fed with high-fat diet and then injected with isoproterenol.According to ECG,80 rats were successfully established.Then they were randomly divided into model group,acupuncture combined with Chinese medicine group,acupuncture group and Western medicine group.The content of bFGF protein was measured by ELISA.The protein contents of Kv1.4,Kv4.2,Kv4.3 and KChIP2 were measured by Western blot.Results:At the end of treatment,compared with the model group,the protein contents of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF in each treatment group increased,and the increase was most significant in the acupuncture combined with Chinese medicine group(P<0.05).At the end of treatment,compared with the model group,the protein contents of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF in each treatment group increased,and the increase was most significant in the acupuncture combined with Chinese medicine group(P<0.05).Compared with the treatment group at the end of treatment,the expression of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF protein in each treatment group 30 days after the end of treatment decreased slightly(P<0.05),but still higher than that of the model group at this time(P<0.05).The combination of acupuncture and Chinese medicine group decreased the least of them(P<0.05).Conclusion:The results showed that acupuncture combined with xijingtongmai decoction had a sustained good effect.Its sustained action mechanism may be achieved by continuously increasing the protein content of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF through transient outward potassium channel.
基金supported in part by NSFC (no. 30700151)Academic Innovation Incubation Program from UESTC (no. Y02018023601062)Some data have been published in Journal of Nanoscience and Nanotechnology (2009, 9: 282-287)
文摘Poly (D,L-lactide-co-glycolide) (PLGA) is a biodegradable and biocompatible polymer material for drug deliver system. The aim of this study is to synthesize drug-loaded
基金supported by the National Key Research and Development Program of China (2022YFA0911900)the start-up packages from Westlake Universitythe Research Center for Industries of the Future (RCIF) at Westlake University。
文摘Effective utilization of sustainable energies necessitates the development of electrochemical energy conversion and storage devices,where the catalysts and ion-conducting membranes are two core components.Anion exchange membrane water electrolysis(AEM-WE)has been widely regarded as the next-generation technology for green hydrogen production because it provides a feasible route to achieve non-precious-metal catalyzed green energy conversion[1].Currently,the design of high-performance catalysts for the anodic oxygen evolution reaction(OER)and cathodic hydrogen evolution reaction(HER)has attracted wide attention and made impressive achievements[2],but the development of the indispensable AEM is relatively less mature[3].Therefore,designing AEMs with high conductivity,long-term stability,and mechanical strength represents one of the key challenges to realizing effective and economical water electrolysis with AEM-WE.
