The water quality in the surface microlayer （SML） and subsurface water （SSW） in the Guangzhou segment of Pearl River, a eutrophic urban river section in China, were ana- lyzed. The spatial and temporal dynamics of...The water quality in the surface microlayer （SML） and subsurface water （SSW） in the Guangzhou segment of Pearl River, a eutrophic urban river section in China, were ana- lyzed. The spatial and temporal dynamics of nutrient concentrations, heavy metals and bac- teria were examined from two sampling sites in monthly samples throughout 2010. The mean concentrations of total nitrogen, ammonia, nitrate and nitrite were higher than 7.0 mg/L, 3.1 mg/L, 1.1 mg/L and 0.3 mg/L, while total phosphorus and orthophosphate were 0.5 mg/L and 0.1 mg/L, respectively. These results indicated that the water quality was rich in minerals and eutrophic. The mean concentrations of Mn and Fe were higher than 0.013 mg/L; and Ni, Cr and Pb were higher than 0.001 mg/L. The mean concentrations were in the order of Mn 〉Fe 〉 Ni 〉 Pb 〉 Cr. The concentrations of heavy metals in the Guangzhou segment were lower than the limit of the surface water quality standards in China and the World Health Organization （WHO）, but higher than the median values in the world＇s freshwater. The density of bacteria ranged from 3.30×10^5 to 5.23×10^6 cells/mL, and the amount of cultivable heterotrophic bac-teria ranged from 1.30×10^3 to 1.89×10^6 cfu/mL. Fecal coliform levels were beyond the V class of China water quality standard. The SML was enriched in nutrients, heavy metals and bac- teria, with the maximum enrichment factor of 3.84 for nutrients, 8.00 for heavy metals, and 3.04 for bacteria, suggesting that the water quality of the SML of the Guangzhou segment of the Pearl River was more serious than in the SSW.展开更多
The enrichment of nutrients (Noa-, Noa-, PO43-), suspended particles, organic matter (POC, PON,DOC) , and trace metals (Cu, Ni, Cd) was determined in the sea surface microlayer of Xiamen Bay and Jiulong Estuary. The m...The enrichment of nutrients (Noa-, Noa-, PO43-), suspended particles, organic matter (POC, PON,DOC) , and trace metals (Cu, Ni, Cd) was determined in the sea surface microlayer of Xiamen Bay and Jiulong Estuary. The mean enrichment factors ([Xi]microlayer/[Xi ]15cm in depth) mostly ranged between 1 . 0 and 2. 0. The dissolved forms were the major forms of the components measured, the enrichment of dissolved organic matter and suspended particles could lead to the changes in the total amount and speciation of nutrients and trace metals. No correlation was observed between sample concentrations, speciation, enrichment factors and sample locations. However, some evidence shows that these parameters are correlated with sea state, indicating the complexity and dynamic nature of the sea surface microlayer.展开更多
This study on the temporal and spatial variability of the viscosity and some chemical parameters in the sea surface microlayer (SML), the relationship between the viscosity and chemical parameters, and the influence o...This study on the temporal and spatial variability of the viscosity and some chemical parameters in the sea surface microlayer (SML), the relationship between the viscosity and chemical parameters, and the influence of the viscosity on the mass transfer coefficient ( K ) in the flux of materials through the air sea interface revealed that: The values of viscosity and some chemical parameters in the SML are higher than those in the sub surface layer (SSL), and at daytime are higher than those at night. The viscosity has positive corelation with chemical oxygen demand (COD), dissolved organic carbon (DOC) and salinity. The "SML effect" on K need not be considered because the SML effect on materials concentration is so small.展开更多
Glass-plate sampling during 1988-1999 in Daya Bay and suitable corresponding analytical methods were used for the measurement of dissolved trace metals, dissolved organic carbon, biological oxygen demand, chemical oxy...Glass-plate sampling during 1988-1999 in Daya Bay and suitable corresponding analytical methods were used for the measurement of dissolved trace metals, dissolved organic carbon, biological oxygen demand, chemical oxygen demand, salinity of the sea surface microlayer and subsurface water. Apparent enrichment mechanism and diurnal variation have been revealed for dissolved trace metals in the microlayer in Daya Bay. The more dissolved organic matter was enriched in the sea surface microlayer, the more dissolved trace metals were enriched in the layer. The organic matter played an important role in the enrichment process. The diurnal variations of dissolved trace metals showed that their concentration was apparently inversely related to the tide activity that the concentration was low during rising tide, but high during falling tide. The behavior of dissolved trace metals expressed by the diurnal variation was clearly opposite to that of salinity.展开更多
The authors’ surveys in May-June 1999 (two cruises) at six sampling stations near nuclear power plants (NPP) and marine fish culture zones in Daya Bay, Guangdong, revealed species composition, densities and body-size...The authors’ surveys in May-June 1999 (two cruises) at six sampling stations near nuclear power plants (NPP) and marine fish culture zones in Daya Bay, Guangdong, revealed species composition, densities and body-size of the sea surface microlayer (SM) zooplankton (>35 μm). Results showed that protozoans and copepod nauplii were the predominant components, accounting for 65.40% to 95.56% of total zooplankton in abundance. The size-frequency distributions showed that the frequency of micro-zooplankton (0.02-0.2 mm) reached 0.8235. The SM zooplankton community structure revealed in the present study was quite different from that revealed by investigations in the 1980s in Daya Bay. Difference of sampling method has important influence on the obtained zooplankton community structure. SM zooplankton consisted of micro- and mesozooplankton (0.2-2.0 mm), with micro-zooplankton being predominant. Some possible cause-effect relations between the zooplankton community structure and mariculture, nuclear power plants cooling systems and sampling method are discussed.展开更多
Enrichment of volatile organic compounds(VOC) and semi-volatility organic compounds(SVOC) in surface microlayer(SM) of three drinking water sources were studied. The enrichment factor( EF s) were 0.67 to 13.37 and 0.1...Enrichment of volatile organic compounds(VOC) and semi-volatility organic compounds(SVOC) in surface microlayer(SM) of three drinking water sources were studied. The enrichment factor( EF s) were 0.67 to 13.37 and 0.16 to 136, respectively. The results showed some VOC and most SVOC could enrich in SM. Some EF s of SVOC was quite high. Suspension and temperature could affect EF s of SVOC, slim wind and water movement do not destroy enrichment of organic in SM.展开更多
Copper complexing ligands concentration (C C) and conditional stability constant of the sea surface microlayer (SML) and subsurface layer (SSL) samples, collected from Daya Bay of Guangdong Province and Jiaozhou Bay o...Copper complexing ligands concentration (C C) and conditional stability constant of the sea surface microlayer (SML) and subsurface layer (SSL) samples, collected from Daya Bay of Guangdong Province and Jiaozhou Bay of Shandong Province, were determined by anodic stripping voltammetry (ASV) technique. C C of Daya Bay in the SML ranged from 6.19×10-7 to 3.52×10-7 mol/L. C C of Jiaozhou Bay in the SML ranged from 5.30×10-7 to 3.03×10-7 mol/L in August 1998 and from 3.46×10-7 to 1.36×10-7 mol/L in May 2000. The C C concentrations were higher in all surface microlayer samples, compared with corresponding subsurface samples. The average enrichment factors of copper complexation in the SML observed above were 1.58, 1.41 and 1.56, respectively. The enrichment of C C concentration in the microlayer demonstrated that organic ligands had certain buffer action on the toxicity of the enriched trace metals in the same layer. Conditional stability constants of the SML in Jiaozhou Bay were lower than those of the SSL; however, it showed just opposite for Daya Bay. BOD (biological oxygen demand) and COD (chemical oxygen demand) indicated the organic matter content, also showed enriched in the SML. C C had an obvious relationship with BOD or COD.展开更多
The surface microlayer(SML)is a thin biogenic film found between the marine environment and troposphere that comprises a unique marine ecosystem with its own physicochemical and microbiological compositions.In this st...The surface microlayer(SML)is a thin biogenic film found between the marine environment and troposphere that comprises a unique marine ecosystem with its own physicochemical and microbiological compositions.In this study,we compared the bacterial cell abundance and community structure of the SML and subsurface waters(0.50m depth)using traditional culture and flow cytometry methods.Overall,53strains were screened and sequenced.In addition,DNA was extracted from the SML and corresponding subsurface water and used for bacterial community analysis by denaturing gradient gel electrophoresis(DGGE).The results revealed that the SML had a distinct microbial community structure from the corresponding subsurface water.This is the first compresensively detailed molecular biological analysis of the bacterioneuston in the Yellow Sea of China.展开更多
Two cruises for multi discipline investigation were conducted in October and November, 1998 in Daya Bay to collect: (1) samples from five levels including sea surface microlayer (SML), subsurface (SSL), surface, body ...Two cruises for multi discipline investigation were conducted in October and November, 1998 in Daya Bay to collect: (1) samples from five levels including sea surface microlayer (SML), subsurface (SSL), surface, body (mid column) and bottom water; and (2) observational data on chlorophyll a and nutrients, and important factors such as turbidity, BOD, COD, TN, TP and phytoplankton. Results showed that there was no enrichment of chlorophyll a in the SML, but quite notable enrichment of some organic matter and nutrients. Compared with the findings in earlier researches, PO 4 P and SiO 3 Si have decreased, and the N/P ratio has increased obviously to even more than 100 in the SML. It is concluded preliminarily that PO 4 P and SiO 3 Si mainly determine phytoplankton growth in Daya Bay at present.展开更多
Surface water can be divided into three layers from top downward： surface microlayer （SML, thickness≤50 μm）, subsurface layer （SSL, ≈25 cm） and surface layer （SL, l-5m）, among which the SML plays an importan...Surface water can be divided into three layers from top downward： surface microlayer （SML, thickness≤50 μm）, subsurface layer （SSL, ≈25 cm） and surface layer （SL, l-5m）, among which the SML plays an important role on sea-air interaction because of its unique physical-chemical property. Carbon dioxide system including DIC （dissolved inorganic carbon）, Alk （alkalinity）, pH and pCO： （partial pressure of CO2） in multilayered waters of the Yellow Sea was studied for the first time in March and May 2005. The results show that： DIC and Alk are obviously enriched in SML. The contents ofDIC, Alk and pCO2 become lower in turn from SML, SSL to SL, higher in March and lower in May, whereas for pH it was opposite. The relationship between DIC and Alk is clearly positive, but negative between pH and pCO2. Meanwhile, pCO2 and temperature/salinity is also in positive relation, pCO2 decreases with latitude increase. DIC and Alk show a similar variation trend with the maximum at 02：00-03：00, but pH and pCO2 show an opposite pattern. In addition, the distribution patterns are similar to each other in the three layers. The Yellow Sea is shown to be a sink of atmospheric CO2 in spring by two methods： （1） comparing pCO2 in seawater and atmosphere; （2） turning direction of ＂pH-depth＂ curve. Calculation on the base of pCO2 data in SML in four models shows that carbon flux in spring in the area was about -6.96×10^6 t C.展开更多
Three fractions of alkaline phosphatase activity(APA),including phytoplankton APA(phyto-APA),bacterial APA(bact-APA),and free-APA,were examined in the sea surface microlayer(SML)and the subsurface water(SSW)from Daya ...Three fractions of alkaline phosphatase activity(APA),including phytoplankton APA(phyto-APA),bacterial APA(bact-APA),and free-APA,were examined in the sea surface microlayer(SML)and the subsurface water(SSW)from Daya Bay,Guishan Island,and Guanghai Bay of southern China.Relationships between APA and environmental parameters were analyzed.The growth of phytoplankton was significantly limited by dissolved inorganic phosphorus(DIP)in the three sea areas,especially in Daya Bay.TotalAPA ranged between 1.41 and 35.26 nmol/L/hr,and the highest value was found in Daya Bay.The increased APA in Daya Bay was the result of the increase of phytoplankton biomass and the response of phytoplankton to P limitation.Phyto-APA was the main contributor in Daya Bay,while phyto-and free-APA co-dominated in Guishan Island and Guanghai Bay.Bact-,phyto-,and total-APA showed a significant inverse power function relationship with DIP,and 0.2μmol/L was the threshold for DIP on particulates and totalAPA.Pearson correlation analysis suggested that DIP limitation together with high N levels enhanced APA.High water temperature and freshwater input accelerated APA as well.Principal component analysis clearly separated samples from the three sea areas,as well as from the SML and the SSW,which indicated the differences in environmental parameters and APA levels.Our results highlight the influence of phosphorus limitation and environmental parameters on APA.展开更多
The sea surface microlayer(SML)is the thin boundary layer between the ocean and the atmosphere.Phytoplankton community in the SML is known as phytoneuston and has a different community structure and biomass from the p...The sea surface microlayer(SML)is the thin boundary layer between the ocean and the atmosphere.Phytoplankton community in the SML is known as phytoneuston and has a different community structure and biomass from the phytoplankton of the underlying bulk water.In this study,water samples were collected from the SML of Daya Bay in southern China in September,October,and December of 2013.Algal growth potential(AGP)tests were conducted by adding different forms of nitrogen(N),phosphorus(P)and silicate(Si).Phytoneuston grew well with both inorganic and organic nutrients,and showed comparable community structure after nutrient addition.A total of 16 pigments were analyzed in the SML samples collected from Daya Bay in this study.Phytopigments were dominated by the cyanobacteria-diagnostic pigment(zeaxanthin,Zea)in September and October,and by diatom-diagnostic pigment(fucoxanthin,Fuco)in December.The concentrations and proportions of Fuco increased greatly after the nutrient addition,and the concentrations and proportions of Zea decreased accordingly.Additionally,the diatom pigment index(DiatDP)increased after nutrient addition,and the prokaryotic pigment index(ProkDP)conversely decreased.The results suggested that massive occurrences of Zea and cyanobacteria are important characteristics of phytoneuston structure in Daya Bay especially in warm seasons,and nutrient addition especially Si addition promoted the growth of diatoms.展开更多
Characteristics of interaction between di-2-ethylhexyl phthalate(DEHP) and particulate in a eutrophic lake were studied in this paper. DEHP concentrations ranged from 89.9 to 247 μg/L with an average value of 146 μg...Characteristics of interaction between di-2-ethylhexyl phthalate(DEHP) and particulate in a eutrophic lake were studied in this paper. DEHP concentrations ranged from 89.9 to 247 μg/L with an average value of 146 μg/L in subsurface water (SSW) samples, and from 82.0 to 390 μg/L with an average value of 211 μg/L in water surface microlayer (SM) samples. The results indicate that there was only a weak correlation between the DEHP concentrations and suspended particulate material(SPM) concentrations in both SSW and SM, while the significant correlation between DEHP concentrations and chlorophyll a concentrations was found, which suggestes that DEHP was principally bound to phytoplankton in the eutrophic lake. Correlation between DEHP concentrations and total phosphor (TP) concentrations was also found in this investigation.Enrichment factors (EF) of DEHP in SM were in the range of 0.85 to 2.12 with an average value of 1.35. DEHP EFs were significantly related to the enrichment of chlorophyll a in SM. The results suggest that the enrichment of DEHP in SM of this eutrophic lake was mainly due to DEHP accumulation in phytoplankton and was controlled by distribution of phytoplankton between SM and SSW.展开更多
The majority of marine ammonia oxidizers belong to Thaumarchaeota,a phylum of Archaea,which is distributed throughout the water column.Marine surface waters contain distinct thaumarchaeotal phylotypes compared to the ...The majority of marine ammonia oxidizers belong to Thaumarchaeota,a phylum of Archaea,which is distributed throughout the water column.Marine surface waters contain distinct thaumarchaeotal phylotypes compared to the deeper ocean,but spatial dynamics of the surface-associated lineages are largely unsolved.This study of 120 seawater samples from the eastern Chinese marginal seas identifed contrasting distribution and association patterns among thaumarchaeotal phylotypes across diferent dimensions.Horizontally,Nitrosopumilus-like and Nitrosopelagicus-like phylotypes dominated the surface water(3 m)of the Yellow Sea(YS)and East China Sea(ECS),respectively,along with increased abundance of total free-living Thaumarchaeota in ECS.Similar compositional changes were observed in the surface microlayer.The spatial heterogeneity of particle-attached Thaumarchaeota was less clear in surface microlayers than in surface waters.Vertically,the Nitrosopelagicus-like phylotype increased in abundance from surface to 90 m in ECS,which led to an increase in the proportion of Thaumarchaeota relative to total prokaryotes.This occurred mainly in the free-living fraction.These results indicate a clear size-fractionated niche partitioning,which is more pronounced at lower depths than in the surface water/surface microlayer.In addition,associations of Thaumarchaeota with other microbial taxa varied between phylotypes and size fractions.Our results show that a phylotype-resolved and size-fractionated spatial heterogeneity of the thaumarchaeotal community is present in surface oceanic waters and a vertical variation of the Nitrosopelagicus-like phylotype is present in shallow shelf waters.展开更多
基金Foundation： National Natural Science Foundation of China, No.41173079 The Project of Department of Science and Technology, China, No.2012BAC07B05, No.2012BAD18B01 Jinan University Project, No.21612324
文摘The water quality in the surface microlayer （SML） and subsurface water （SSW） in the Guangzhou segment of Pearl River, a eutrophic urban river section in China, were ana- lyzed. The spatial and temporal dynamics of nutrient concentrations, heavy metals and bac- teria were examined from two sampling sites in monthly samples throughout 2010. The mean concentrations of total nitrogen, ammonia, nitrate and nitrite were higher than 7.0 mg/L, 3.1 mg/L, 1.1 mg/L and 0.3 mg/L, while total phosphorus and orthophosphate were 0.5 mg/L and 0.1 mg/L, respectively. These results indicated that the water quality was rich in minerals and eutrophic. The mean concentrations of Mn and Fe were higher than 0.013 mg/L; and Ni, Cr and Pb were higher than 0.001 mg/L. The mean concentrations were in the order of Mn 〉Fe 〉 Ni 〉 Pb 〉 Cr. The concentrations of heavy metals in the Guangzhou segment were lower than the limit of the surface water quality standards in China and the World Health Organization （WHO）, but higher than the median values in the world＇s freshwater. The density of bacteria ranged from 3.30×10^5 to 5.23×10^6 cells/mL, and the amount of cultivable heterotrophic bac-teria ranged from 1.30×10^3 to 1.89×10^6 cfu/mL. Fecal coliform levels were beyond the V class of China water quality standard. The SML was enriched in nutrients, heavy metals and bac- teria, with the maximum enrichment factor of 3.84 for nutrients, 8.00 for heavy metals, and 3.04 for bacteria, suggesting that the water quality of the SML of the Guangzhou segment of the Pearl River was more serious than in the SSW.
文摘The enrichment of nutrients (Noa-, Noa-, PO43-), suspended particles, organic matter (POC, PON,DOC) , and trace metals (Cu, Ni, Cd) was determined in the sea surface microlayer of Xiamen Bay and Jiulong Estuary. The mean enrichment factors ([Xi]microlayer/[Xi ]15cm in depth) mostly ranged between 1 . 0 and 2. 0. The dissolved forms were the major forms of the components measured, the enrichment of dissolved organic matter and suspended particles could lead to the changes in the total amount and speciation of nutrients and trace metals. No correlation was observed between sample concentrations, speciation, enrichment factors and sample locations. However, some evidence shows that these parameters are correlated with sea state, indicating the complexity and dynamic nature of the sea surface microlayer.
文摘This study on the temporal and spatial variability of the viscosity and some chemical parameters in the sea surface microlayer (SML), the relationship between the viscosity and chemical parameters, and the influence of the viscosity on the mass transfer coefficient ( K ) in the flux of materials through the air sea interface revealed that: The values of viscosity and some chemical parameters in the SML are higher than those in the sub surface layer (SSL), and at daytime are higher than those at night. The viscosity has positive corelation with chemical oxygen demand (COD), dissolved organic carbon (DOC) and salinity. The "SML effect" on K need not be considered because the SML effect on materials concentration is so small.
文摘Glass-plate sampling during 1988-1999 in Daya Bay and suitable corresponding analytical methods were used for the measurement of dissolved trace metals, dissolved organic carbon, biological oxygen demand, chemical oxygen demand, salinity of the sea surface microlayer and subsurface water. Apparent enrichment mechanism and diurnal variation have been revealed for dissolved trace metals in the microlayer in Daya Bay. The more dissolved organic matter was enriched in the sea surface microlayer, the more dissolved trace metals were enriched in the layer. The organic matter played an important role in the enrichment process. The diurnal variations of dissolved trace metals showed that their concentration was apparently inversely related to the tide activity that the concentration was low during rising tide, but high during falling tide. The behavior of dissolved trace metals expressed by the diurnal variation was clearly opposite to that of salinity.
文摘The authors’ surveys in May-June 1999 (two cruises) at six sampling stations near nuclear power plants (NPP) and marine fish culture zones in Daya Bay, Guangdong, revealed species composition, densities and body-size of the sea surface microlayer (SM) zooplankton (>35 μm). Results showed that protozoans and copepod nauplii were the predominant components, accounting for 65.40% to 95.56% of total zooplankton in abundance. The size-frequency distributions showed that the frequency of micro-zooplankton (0.02-0.2 mm) reached 0.8235. The SM zooplankton community structure revealed in the present study was quite different from that revealed by investigations in the 1980s in Daya Bay. Difference of sampling method has important influence on the obtained zooplankton community structure. SM zooplankton consisted of micro- and mesozooplankton (0.2-2.0 mm), with micro-zooplankton being predominant. Some possible cause-effect relations between the zooplankton community structure and mariculture, nuclear power plants cooling systems and sampling method are discussed.
文摘Enrichment of volatile organic compounds(VOC) and semi-volatility organic compounds(SVOC) in surface microlayer(SM) of three drinking water sources were studied. The enrichment factor( EF s) were 0.67 to 13.37 and 0.16 to 136, respectively. The results showed some VOC and most SVOC could enrich in SM. Some EF s of SVOC was quite high. Suspension and temperature could affect EF s of SVOC, slim wind and water movement do not destroy enrichment of organic in SM.
基金Supported by subproject of the China’s "Ninth Five Year Plan" Scientific Research Project No. 97-926-04-03 the Natural Science Foundation of China (No. 40076020, 40376022) the Doctoral Research Program of Higher Education (No. 98042304) and KZ95T-04-04-04 Fund.
文摘Copper complexing ligands concentration (C C) and conditional stability constant of the sea surface microlayer (SML) and subsurface layer (SSL) samples, collected from Daya Bay of Guangdong Province and Jiaozhou Bay of Shandong Province, were determined by anodic stripping voltammetry (ASV) technique. C C of Daya Bay in the SML ranged from 6.19×10-7 to 3.52×10-7 mol/L. C C of Jiaozhou Bay in the SML ranged from 5.30×10-7 to 3.03×10-7 mol/L in August 1998 and from 3.46×10-7 to 1.36×10-7 mol/L in May 2000. The C C concentrations were higher in all surface microlayer samples, compared with corresponding subsurface samples. The average enrichment factors of copper complexation in the SML observed above were 1.58, 1.41 and 1.56, respectively. The enrichment of C C concentration in the microlayer demonstrated that organic ligands had certain buffer action on the toxicity of the enriched trace metals in the same layer. Conditional stability constants of the SML in Jiaozhou Bay were lower than those of the SSL; however, it showed just opposite for Daya Bay. BOD (biological oxygen demand) and COD (chemical oxygen demand) indicated the organic matter content, also showed enriched in the SML. C C had an obvious relationship with BOD or COD.
基金supported by the Natural Science Foundation of China (No. 41406129)the National Key Research Program (Grant 2016YFA0601400)the State Oceanic Administration of China(Grant GASI-03-01-02-05)
文摘The surface microlayer(SML)is a thin biogenic film found between the marine environment and troposphere that comprises a unique marine ecosystem with its own physicochemical and microbiological compositions.In this study,we compared the bacterial cell abundance and community structure of the SML and subsurface waters(0.50m depth)using traditional culture and flow cytometry methods.Overall,53strains were screened and sequenced.In addition,DNA was extracted from the SML and corresponding subsurface water and used for bacterial community analysis by denaturing gradient gel electrophoresis(DGGE).The results revealed that the SML had a distinct microbial community structure from the corresponding subsurface water.This is the first compresensively detailed molecular biological analysis of the bacterioneuston in the Yellow Sea of China.
文摘Two cruises for multi discipline investigation were conducted in October and November, 1998 in Daya Bay to collect: (1) samples from five levels including sea surface microlayer (SML), subsurface (SSL), surface, body (mid column) and bottom water; and (2) observational data on chlorophyll a and nutrients, and important factors such as turbidity, BOD, COD, TN, TP and phytoplankton. Results showed that there was no enrichment of chlorophyll a in the SML, but quite notable enrichment of some organic matter and nutrients. Compared with the findings in earlier researches, PO 4 P and SiO 3 Si have decreased, and the N/P ratio has increased obviously to even more than 100 in the SML. It is concluded preliminarily that PO 4 P and SiO 3 Si mainly determine phytoplankton growth in Daya Bay at present.
基金Supported by the Major Project of the NSFC (No.40490263)the NSFC (No.40076020, 40376022)+1 种基金the National Basic Research Program of China (973 Program) (No.2001CB409700)the Doctoral Program for Higher Education (No.20030423007).
文摘Surface water can be divided into three layers from top downward： surface microlayer （SML, thickness≤50 μm）, subsurface layer （SSL, ≈25 cm） and surface layer （SL, l-5m）, among which the SML plays an important role on sea-air interaction because of its unique physical-chemical property. Carbon dioxide system including DIC （dissolved inorganic carbon）, Alk （alkalinity）, pH and pCO： （partial pressure of CO2） in multilayered waters of the Yellow Sea was studied for the first time in March and May 2005. The results show that： DIC and Alk are obviously enriched in SML. The contents ofDIC, Alk and pCO2 become lower in turn from SML, SSL to SL, higher in March and lower in May, whereas for pH it was opposite. The relationship between DIC and Alk is clearly positive, but negative between pH and pCO2. Meanwhile, pCO2 and temperature/salinity is also in positive relation, pCO2 decreases with latitude increase. DIC and Alk show a similar variation trend with the maximum at 02：00-03：00, but pH and pCO2 show an opposite pattern. In addition, the distribution patterns are similar to each other in the three layers. The Yellow Sea is shown to be a sink of atmospheric CO2 in spring by two methods： （1） comparing pCO2 in seawater and atmosphere; （2） turning direction of ＂pH-depth＂ curve. Calculation on the base of pCO2 data in SML in four models shows that carbon flux in spring in the area was about -6.96×10^6 t C.
基金supported by the Science & Technology Basic Resources Investigation Program of China (No. 2018FY100200)
文摘Three fractions of alkaline phosphatase activity(APA),including phytoplankton APA(phyto-APA),bacterial APA(bact-APA),and free-APA,were examined in the sea surface microlayer(SML)and the subsurface water(SSW)from Daya Bay,Guishan Island,and Guanghai Bay of southern China.Relationships between APA and environmental parameters were analyzed.The growth of phytoplankton was significantly limited by dissolved inorganic phosphorus(DIP)in the three sea areas,especially in Daya Bay.TotalAPA ranged between 1.41 and 35.26 nmol/L/hr,and the highest value was found in Daya Bay.The increased APA in Daya Bay was the result of the increase of phytoplankton biomass and the response of phytoplankton to P limitation.Phyto-APA was the main contributor in Daya Bay,while phyto-and free-APA co-dominated in Guishan Island and Guanghai Bay.Bact-,phyto-,and total-APA showed a significant inverse power function relationship with DIP,and 0.2μmol/L was the threshold for DIP on particulates and totalAPA.Pearson correlation analysis suggested that DIP limitation together with high N levels enhanced APA.High water temperature and freshwater input accelerated APA as well.Principal component analysis clearly separated samples from the three sea areas,as well as from the SML and the SSW,which indicated the differences in environmental parameters and APA levels.Our results highlight the influence of phosphorus limitation and environmental parameters on APA.
基金the Science&Technology Basic Resources Investigation Program of China(No.2018FY100200).
文摘The sea surface microlayer(SML)is the thin boundary layer between the ocean and the atmosphere.Phytoplankton community in the SML is known as phytoneuston and has a different community structure and biomass from the phytoplankton of the underlying bulk water.In this study,water samples were collected from the SML of Daya Bay in southern China in September,October,and December of 2013.Algal growth potential(AGP)tests were conducted by adding different forms of nitrogen(N),phosphorus(P)and silicate(Si).Phytoneuston grew well with both inorganic and organic nutrients,and showed comparable community structure after nutrient addition.A total of 16 pigments were analyzed in the SML samples collected from Daya Bay in this study.Phytopigments were dominated by the cyanobacteria-diagnostic pigment(zeaxanthin,Zea)in September and October,and by diatom-diagnostic pigment(fucoxanthin,Fuco)in December.The concentrations and proportions of Fuco increased greatly after the nutrient addition,and the concentrations and proportions of Zea decreased accordingly.Additionally,the diatom pigment index(DiatDP)increased after nutrient addition,and the prokaryotic pigment index(ProkDP)conversely decreased.The results suggested that massive occurrences of Zea and cyanobacteria are important characteristics of phytoneuston structure in Daya Bay especially in warm seasons,and nutrient addition especially Si addition promoted the growth of diatoms.
文摘Characteristics of interaction between di-2-ethylhexyl phthalate(DEHP) and particulate in a eutrophic lake were studied in this paper. DEHP concentrations ranged from 89.9 to 247 μg/L with an average value of 146 μg/L in subsurface water (SSW) samples, and from 82.0 to 390 μg/L with an average value of 211 μg/L in water surface microlayer (SM) samples. The results indicate that there was only a weak correlation between the DEHP concentrations and suspended particulate material(SPM) concentrations in both SSW and SM, while the significant correlation between DEHP concentrations and chlorophyll a concentrations was found, which suggestes that DEHP was principally bound to phytoplankton in the eutrophic lake. Correlation between DEHP concentrations and total phosphor (TP) concentrations was also found in this investigation.Enrichment factors (EF) of DEHP in SM were in the range of 0.85 to 2.12 with an average value of 1.35. DEHP EFs were significantly related to the enrichment of chlorophyll a in SM. The results suggest that the enrichment of DEHP in SM of this eutrophic lake was mainly due to DEHP accumulation in phytoplankton and was controlled by distribution of phytoplankton between SM and SSW.
基金We thank the scientists and crews on the R/V Dongfanghong 2 for their assistance with sampling during the cruises.We thank Chunying Liu and Guipeng Yang both of the Ocean University of China for providing pH and DO data,respectively.This work was funded by the National Natural Science Foundation of China(92051115,41976101,92251303 and 41730530)the Scientifc and Technological Innovation Project of Laoshan Laboratory(LSKJ202203206 and LSKJ202203201)+2 种基金the Shandong Provincial Natural Science Foundation(ZR2022YQ38)the National Key Research and Development Program of China(2018YFE0124100)the Fundamental Research Funds for the Central Universities(202141009 and 202172002).
文摘The majority of marine ammonia oxidizers belong to Thaumarchaeota,a phylum of Archaea,which is distributed throughout the water column.Marine surface waters contain distinct thaumarchaeotal phylotypes compared to the deeper ocean,but spatial dynamics of the surface-associated lineages are largely unsolved.This study of 120 seawater samples from the eastern Chinese marginal seas identifed contrasting distribution and association patterns among thaumarchaeotal phylotypes across diferent dimensions.Horizontally,Nitrosopumilus-like and Nitrosopelagicus-like phylotypes dominated the surface water(3 m)of the Yellow Sea(YS)and East China Sea(ECS),respectively,along with increased abundance of total free-living Thaumarchaeota in ECS.Similar compositional changes were observed in the surface microlayer.The spatial heterogeneity of particle-attached Thaumarchaeota was less clear in surface microlayers than in surface waters.Vertically,the Nitrosopelagicus-like phylotype increased in abundance from surface to 90 m in ECS,which led to an increase in the proportion of Thaumarchaeota relative to total prokaryotes.This occurred mainly in the free-living fraction.These results indicate a clear size-fractionated niche partitioning,which is more pronounced at lower depths than in the surface water/surface microlayer.In addition,associations of Thaumarchaeota with other microbial taxa varied between phylotypes and size fractions.Our results show that a phylotype-resolved and size-fractionated spatial heterogeneity of the thaumarchaeotal community is present in surface oceanic waters and a vertical variation of the Nitrosopelagicus-like phylotype is present in shallow shelf waters.
