Ce(Ⅲ)-ALC-F complex can react with hsDNA to form an electrochemically non-active supermolecular complex Ce(Ⅲ)-ALC-F-DNA in the buffer solution of (CH2)6N4(pH=4.9), which results in the decrease of the peak current o...Ce(Ⅲ)-ALC-F complex can react with hsDNA to form an electrochemically non-active supermolecular complex Ce(Ⅲ)-ALC-F-DNA in the buffer solution of (CH2)6N4(pH=4.9), which results in the decrease of the peak current of Ce(Ⅲ)-ALC-F. This method can be applied to determine DNA concentration. In addition, by using fluorimetric and UV-spectrophotometric methods with studies of denatured DNA and the effect of NaCl solution , it is also found that the binding mode is intercalation.展开更多
文摘Ce(Ⅲ)-ALC-F complex can react with hsDNA to form an electrochemically non-active supermolecular complex Ce(Ⅲ)-ALC-F-DNA in the buffer solution of (CH2)6N4(pH=4.9), which results in the decrease of the peak current of Ce(Ⅲ)-ALC-F. This method can be applied to determine DNA concentration. In addition, by using fluorimetric and UV-spectrophotometric methods with studies of denatured DNA and the effect of NaCl solution , it is also found that the binding mode is intercalation.
