Background:Breast cancer is the most common cancer in women,and in advanced stages,it often metastasizes to the brain.However,research on the biological mechanisms of breast cancer brain metastasis and potential thera...Background:Breast cancer is the most common cancer in women,and in advanced stages,it often metastasizes to the brain.However,research on the biological mechanisms of breast cancer brain metastasis and potential therapeutic targets are limited.Methods:Differential gene expression analysis(DEGs)for the datasets GSE43837 and GSE125989 from the GEO database was performed using online analysis tools such as GEO2R and Sangerbox.Further investigation related to SULF1 was conducted using online databases such as Kaplan-Meier Plotter and cBioPortal.Thus,expression levels,variations,associations with HER2,biological processes,and pathways involv-ing SULF1 could be analyzed using UALCAN,cBioPortal,GEPIA2,and LinkedOmics databases.Moreover,the sensitivity of SULF1 to existing drugs was explored using drug databases such as RNAactDrug and CADSP.Results:High expression of SULF1 was associated with poor prognosis in advanced breast cancer brain metastasis and was positively correlated with the expression of HER2.In the metastatic breast cancer population,SULF1 ranked top among the 16 DEGs with the highest mutation rate,reaching 11%,primarily due to amplification.KEGG and GSEA analyses revealed that the genes co-expressed with SULF1 were positively enriched in the‘ECM-receptor interaction’gene set and negatively enriched in the‘Ribosome’gene set.Currently,docetaxel and vinorelbine can act as treatment options if the expression of SULF1 is high.Conclusions:This study,through bioinformatics analysis,unveiled SULF1 as a poten-tial target for treating breast cancer brain metastasis(BM).展开更多
Introduction:Sulfatase 2(SULF2),an endogenous extracellular sulfatase,can remove 6-O-sulfate groups of glucosamine residues from heparan sulfate(HS)chains to modulate the Wnt/β-catenin signaling pathway,which plays a...Introduction:Sulfatase 2(SULF2),an endogenous extracellular sulfatase,can remove 6-O-sulfate groups of glucosamine residues from heparan sulfate(HS)chains to modulate the Wnt/β-catenin signaling pathway,which plays an important role in both liver carcinogenesis and embryogenesis.Side population(SP)cells are widely identified as stem-like cancer cells and are closely related to carcinoma metastasis,recurrence,and poor patient prognosis.However,the roles of SULF2 in SP cells of hepatomas are unclear,and the underlying mechanism is undefined.Objectives:This study aimed to compare the heterogeneity between SP cells and non-side population(NSP)cells derived from three different liver cancer cell lines and to elucidate the involvement of the SULF2-Wnt/β-catenin axis in liver cancer stem cells(CSCs)and its impact on the processes of carcinogenesis and invasiveness.Methods:In this work,three different liver cancer SP cells(HepG2,Huh7,and PRC/PRL/5)were sorted by flow cytometry.We also examined the migration and invasion behaviors of SP and NSP cells.To determine if this high tumorigenic potential of SP cells is correlated to SULF2,qPCR,western blotting,and immunofluorescence analysis were conducted.We also performed nude mouse xenograft experiments for in vivo analysis.Results:The results from the in vitro colony formation assay showed that SP cells exhibited a 2-fold higher colony formation efficiency compared to their NSP counterparts.The SP cells exhibited significantly higher potentials in terms of their migratory capacity and invasive ability compared to NSP cells.We found that higher expression of SULF2 in SP cells was associated with greater capabilities for clonogenicity,migration,and invasion.It was also linked to higher activation of the Wnt/β-catenin signaling pathway via stimulation of key downstream factors,particularlyβ-catenin,c-Myc,and cyclin D1.Further,a positive correlation between the upregulated SULF2 expression and tumorigenesis in the in vivo nude mouse xenograft models was demonstrated,highlighting that the potential underlying mechanism was Wnt/β-catenin signaling pathway activation.Conclusion:Our findings show that variable SULF2 expression was associated with differential activation of the Wnt/β-catenin signaling pathway,which could lead to behavioral differences between SP and NSP cells and also among the SP cells of the three liver cancer cell lines assessed.It was reasonably concluded that the SULF2-Wnt/β-catenin axis could play an important role in the tumorigenicity of liver cancer stem cells.展开更多
<Abstrat>The surface sediment samples were collected month by month at nine stations in the Daya Bay from January to December 1987, and the number of anaerobic sulfite reducing bacteria and their spores and the ...<Abstrat>The surface sediment samples were collected month by month at nine stations in the Daya Bay from January to December 1987, and the number of anaerobic sulfite reducing bacteria and their spores and the regularity of seasonal change were determined. The effect of environmental factors, water temperature and the resoluble oxygen concentration in the bottom of seawater on the number of them were discussed. The results show that the number of anaerobic su|fite reducing bacteria were low in sediment of the Daya Bay, indicating that the hay was less contaminated.展开更多
The invasive pest whitefly(Bemisia tabaci)is a complex species,of which Middle East-Minor Asia 1(MEAM1)and Mediterranean(MED)are the two most damaging members.Previous research showed that cabbage is frequently infest...The invasive pest whitefly(Bemisia tabaci)is a complex species,of which Middle East-Minor Asia 1(MEAM1)and Mediterranean(MED)are the two most damaging members.Previous research showed that cabbage is frequently infested with MEAM1 but seldomly with MED,and this difference in performance is associated with glucosi-nolate(GS)content.Some insects can modify GS using glucosinolate sulfatase(SULF),the activity of which is regulated by sulfatase modifying factor 1(SUMF1);therefore,to increase our understanding of different performances of MEAM1 and MED on cabbage plants,we identified and compared nine putative SULFs and one SUMF in MEAM1 and MED.We found that the lengths of two genes,BtSulf2 and BtSulf4,differed between MEAM1 and MED.The messenger RNA levels of BtSulf4 increased more than 20-fold after MEAMl and MED adults were exposed to GS,but BtSulJ2 expression was only induced by GS in MEAM1.Knockdown of BtSulf2 and BtSulf4 in MEAM1 resulted in a substantial increase in the mortality of GS-treated adults but not in MED.These results indicate that differences in BtSulJ2 and BtSulf4 sequences and/or expression may explain why MEAM1 performs better than MED on cabbage.Our results provide a basis for future functional research on SULF and SUMF in B.tabaci.展开更多
1.China’s sulfuric acid industry developsfastAs of the end of 2009, China had 464 largeand mid-sized sulfuric acid enterprises(counting non-state owned enterprises withannual sales exceeding RMB5 million and allstate...1.China’s sulfuric acid industry developsfastAs of the end of 2009, China had 464 largeand mid-sized sulfuric acid enterprises(counting non-state owned enterprises withannual sales exceeding RMB5 million and allstate-owned enterprises), with a combinedcapacity of 71 million t/a, of which the totalcapacity producing sulfuric acid from sulfurwas about 34.5 million t/a, with the largestsingle-unit capacity being 1 million t/a.Thesmelting flue gas-to-sulfuric acid capacitywas about 19 million t/a, with the biggestunit capable of 700 000 t/a.And the pyrite-to-sulfuric acid capacity was about 17.5 milliont/a, with the biggest unit at 400 000 t/a.So in China, sulfuric acid is basically madefrom sulfur, pyrite or smelting flue gas.Thecombined capacity of large-scale sulfuric acidunits with technology comparable to that ofadvanced countries accounts for over 50%of China’s total sulfuric acid capacity,展开更多
基金Peking Union Medical College,Grant/Award Number:3332022182。
文摘Background:Breast cancer is the most common cancer in women,and in advanced stages,it often metastasizes to the brain.However,research on the biological mechanisms of breast cancer brain metastasis and potential therapeutic targets are limited.Methods:Differential gene expression analysis(DEGs)for the datasets GSE43837 and GSE125989 from the GEO database was performed using online analysis tools such as GEO2R and Sangerbox.Further investigation related to SULF1 was conducted using online databases such as Kaplan-Meier Plotter and cBioPortal.Thus,expression levels,variations,associations with HER2,biological processes,and pathways involv-ing SULF1 could be analyzed using UALCAN,cBioPortal,GEPIA2,and LinkedOmics databases.Moreover,the sensitivity of SULF1 to existing drugs was explored using drug databases such as RNAactDrug and CADSP.Results:High expression of SULF1 was associated with poor prognosis in advanced breast cancer brain metastasis and was positively correlated with the expression of HER2.In the metastatic breast cancer population,SULF1 ranked top among the 16 DEGs with the highest mutation rate,reaching 11%,primarily due to amplification.KEGG and GSEA analyses revealed that the genes co-expressed with SULF1 were positively enriched in the‘ECM-receptor interaction’gene set and negatively enriched in the‘Ribosome’gene set.Currently,docetaxel and vinorelbine can act as treatment options if the expression of SULF1 is high.Conclusions:This study,through bioinformatics analysis,unveiled SULF1 as a poten-tial target for treating breast cancer brain metastasis(BM).
基金supported by the Shenzhen Science and Technology Innovation Commission Fundamental Research Key Projects(Nos.JCYJ20180508153013853,JCYJ20180508152130899)the Shenzhen Science and Technology Innovation Fund(No.JCYJ20150331142757381)the National Natural Science Foundation of China(Nos.81641051,81872368).
文摘Introduction:Sulfatase 2(SULF2),an endogenous extracellular sulfatase,can remove 6-O-sulfate groups of glucosamine residues from heparan sulfate(HS)chains to modulate the Wnt/β-catenin signaling pathway,which plays an important role in both liver carcinogenesis and embryogenesis.Side population(SP)cells are widely identified as stem-like cancer cells and are closely related to carcinoma metastasis,recurrence,and poor patient prognosis.However,the roles of SULF2 in SP cells of hepatomas are unclear,and the underlying mechanism is undefined.Objectives:This study aimed to compare the heterogeneity between SP cells and non-side population(NSP)cells derived from three different liver cancer cell lines and to elucidate the involvement of the SULF2-Wnt/β-catenin axis in liver cancer stem cells(CSCs)and its impact on the processes of carcinogenesis and invasiveness.Methods:In this work,three different liver cancer SP cells(HepG2,Huh7,and PRC/PRL/5)were sorted by flow cytometry.We also examined the migration and invasion behaviors of SP and NSP cells.To determine if this high tumorigenic potential of SP cells is correlated to SULF2,qPCR,western blotting,and immunofluorescence analysis were conducted.We also performed nude mouse xenograft experiments for in vivo analysis.Results:The results from the in vitro colony formation assay showed that SP cells exhibited a 2-fold higher colony formation efficiency compared to their NSP counterparts.The SP cells exhibited significantly higher potentials in terms of their migratory capacity and invasive ability compared to NSP cells.We found that higher expression of SULF2 in SP cells was associated with greater capabilities for clonogenicity,migration,and invasion.It was also linked to higher activation of the Wnt/β-catenin signaling pathway via stimulation of key downstream factors,particularlyβ-catenin,c-Myc,and cyclin D1.Further,a positive correlation between the upregulated SULF2 expression and tumorigenesis in the in vivo nude mouse xenograft models was demonstrated,highlighting that the potential underlying mechanism was Wnt/β-catenin signaling pathway activation.Conclusion:Our findings show that variable SULF2 expression was associated with differential activation of the Wnt/β-catenin signaling pathway,which could lead to behavioral differences between SP and NSP cells and also among the SP cells of the three liver cancer cell lines assessed.It was reasonably concluded that the SULF2-Wnt/β-catenin axis could play an important role in the tumorigenicity of liver cancer stem cells.
文摘<Abstrat>The surface sediment samples were collected month by month at nine stations in the Daya Bay from January to December 1987, and the number of anaerobic sulfite reducing bacteria and their spores and the regularity of seasonal change were determined. The effect of environmental factors, water temperature and the resoluble oxygen concentration in the bottom of seawater on the number of them were discussed. The results show that the number of anaerobic su|fite reducing bacteria were low in sediment of the Daya Bay, indicating that the hay was less contaminated.
基金supported by the National Key R&D Program of China(2019YFD1002100)China Agriculture Research System(CARS-24-C-02)+1 种基金the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS)he Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables.
文摘The invasive pest whitefly(Bemisia tabaci)is a complex species,of which Middle East-Minor Asia 1(MEAM1)and Mediterranean(MED)are the two most damaging members.Previous research showed that cabbage is frequently infested with MEAM1 but seldomly with MED,and this difference in performance is associated with glucosi-nolate(GS)content.Some insects can modify GS using glucosinolate sulfatase(SULF),the activity of which is regulated by sulfatase modifying factor 1(SUMF1);therefore,to increase our understanding of different performances of MEAM1 and MED on cabbage plants,we identified and compared nine putative SULFs and one SUMF in MEAM1 and MED.We found that the lengths of two genes,BtSulf2 and BtSulf4,differed between MEAM1 and MED.The messenger RNA levels of BtSulf4 increased more than 20-fold after MEAMl and MED adults were exposed to GS,but BtSulJ2 expression was only induced by GS in MEAM1.Knockdown of BtSulf2 and BtSulf4 in MEAM1 resulted in a substantial increase in the mortality of GS-treated adults but not in MED.These results indicate that differences in BtSulJ2 and BtSulf4 sequences and/or expression may explain why MEAM1 performs better than MED on cabbage.Our results provide a basis for future functional research on SULF and SUMF in B.tabaci.
文摘1.China’s sulfuric acid industry developsfastAs of the end of 2009, China had 464 largeand mid-sized sulfuric acid enterprises(counting non-state owned enterprises withannual sales exceeding RMB5 million and allstate-owned enterprises), with a combinedcapacity of 71 million t/a, of which the totalcapacity producing sulfuric acid from sulfurwas about 34.5 million t/a, with the largestsingle-unit capacity being 1 million t/a.Thesmelting flue gas-to-sulfuric acid capacitywas about 19 million t/a, with the biggestunit capable of 700 000 t/a.And the pyrite-to-sulfuric acid capacity was about 17.5 milliont/a, with the biggest unit at 400 000 t/a.So in China, sulfuric acid is basically madefrom sulfur, pyrite or smelting flue gas.Thecombined capacity of large-scale sulfuric acidunits with technology comparable to that ofadvanced countries accounts for over 50%of China’s total sulfuric acid capacity,
