The present study is to determine the suitable protoscolices （PSCs） density for long-time culturing in vitro. The PSCs were divided into eight groups with different densities and the viability tests were carried out...The present study is to determine the suitable protoscolices （PSCs） density for long-time culturing in vitro. The PSCs were divided into eight groups with different densities and the viability tests were carried out with 0.1% methylene blue staining. Then the infection ability of cultured PSCs was assessed by the mean cyst weight of mice inoculated intraperitoneally with PSCs after 8 months post-infection.展开更多
Ultra-deep geological conditions are complex, often leading to leakage issues. As the depth increases during ultra-deep bridging lost-circulation operations, the varying settling velocities of different types of lost ...Ultra-deep geological conditions are complex, often leading to leakage issues. As the depth increases during ultra-deep bridging lost-circulation operations, the varying settling velocities of different types of lost circulation materials(LCMs) lead to gravitational differentiation. This causes stratification of the LCMs, forming an unevenly dispersed system and reduced plugging efficacy. An experimental methodology is described for assessing the density suitability between LCMs and drilling fluids, which calculates the density suitability factor(DSF) by measuring the density of the plugging slurry at various locations after a designated resting period, ultimately evaluating its density suitability based on the DSF. Experimental results indicate that LCMs with lower densities demonstrate superior density suitability compared to those with higher densities. Smaller particle sizes(10-40 mesh) show better density suitability than larger ones(2-6 mesh). Fracture-plugging experiments validated this method, the plugging slurry composed of a good density suitability material, such as walnut shells, exhibited no notable gravitational differentiation after 2 h, achieving a maximum plugging pressure of 16.4 MPa with88 m L of total volume loss. In contrast, the slurry formed with poorly density suitability materials, such as calcite, showed significant gravitational differentiation after 2 h, achieving only 9.6 MPa in maximum plugging pressure and a total volume loss of 96 m L. These findings highlight that LCMs with superior density suitability enhance plugging efficiency, highlighting the critical role of density suitability testing and the optimization of plugging formulations to improve the success of plugging operations.展开更多
基金supported by International Collaboration on Drug and Diagnostics Innovation of Tropical Diseases in China (International S&T Cooperation 2010DFB73280)Technology Development Research for Science Research Institute (2011EG150312)the Youth Project of Shanghai Municipal Health Bureau (87)
文摘The present study is to determine the suitable protoscolices （PSCs） density for long-time culturing in vitro. The PSCs were divided into eight groups with different densities and the viability tests were carried out with 0.1% methylene blue staining. Then the infection ability of cultured PSCs was assessed by the mean cyst weight of mice inoculated intraperitoneally with PSCs after 8 months post-infection.
基金support from the National Natural Science Foundation of China(Grant Nos.52274009,51604236)the Sichuan Province Youth Science and technology innovation team project(No.2021JDTD0017)the Sichuan Province Science and Technology Project(Grant No.2018JY0436).
文摘Ultra-deep geological conditions are complex, often leading to leakage issues. As the depth increases during ultra-deep bridging lost-circulation operations, the varying settling velocities of different types of lost circulation materials(LCMs) lead to gravitational differentiation. This causes stratification of the LCMs, forming an unevenly dispersed system and reduced plugging efficacy. An experimental methodology is described for assessing the density suitability between LCMs and drilling fluids, which calculates the density suitability factor(DSF) by measuring the density of the plugging slurry at various locations after a designated resting period, ultimately evaluating its density suitability based on the DSF. Experimental results indicate that LCMs with lower densities demonstrate superior density suitability compared to those with higher densities. Smaller particle sizes(10-40 mesh) show better density suitability than larger ones(2-6 mesh). Fracture-plugging experiments validated this method, the plugging slurry composed of a good density suitability material, such as walnut shells, exhibited no notable gravitational differentiation after 2 h, achieving a maximum plugging pressure of 16.4 MPa with88 m L of total volume loss. In contrast, the slurry formed with poorly density suitability materials, such as calcite, showed significant gravitational differentiation after 2 h, achieving only 9.6 MPa in maximum plugging pressure and a total volume loss of 96 m L. These findings highlight that LCMs with superior density suitability enhance plugging efficiency, highlighting the critical role of density suitability testing and the optimization of plugging formulations to improve the success of plugging operations.
