The trade-off between yield and environmental effects caused by nitrogen fertilizer application is an important issue in wheat production.A reduction in fertile florets is one of the main reasons for the lower yields ...The trade-off between yield and environmental effects caused by nitrogen fertilizer application is an important issue in wheat production.A reduction in fertile florets is one of the main reasons for the lower yields under low nitrogen application rates.Brassinosteroids(BRs)have been found to play a role in nitrogen-induced rice spikelet degeneration.However,whether BRs play a role in wheat floret development and the mechanisms involved are not clear.Therefore,a nitrogen gradient experiment and exogenous spraying experiment were conducted to investigate the role and mechanism of BRs in wheat floret development under low nitrogen stress.The results showed that as the nitrogen application decreased,the endogenous BRs content of the spikes decreased,photosynthesis weakened,and total carbon,soluble sugar and starch in the spikes decreased,leading to a reduction in the number of fertile florets.Under low nitrogen stress,exogenous spraying of 24-epibrassinolide promoted photosynthesis,and stimulated stem fructan hydrolysis and the utilization and storage of sucrose in spikes,which directed more carbohydrates to the spikes and increased the number of fertile florets.In conclusion,BRs mediate the effects of nitrogen fertilizer on wheat floret development,and under low nitrogen stress,foliar spraying of 24-epibrassinolide promotes the flow of carbohydrates from the stem to the spikes,alleviating wheat floret degeneration.展开更多
Sucrose transporters(SUTs)play a crucial role in carbon allocation from the source leaf to the sink end,and the function of SUTs varies among family members.However,the genome-wide identifcation of the SUT superfamily...Sucrose transporters(SUTs)play a crucial role in carbon allocation from the source leaf to the sink end,and the function of SUTs varies among family members.However,the genome-wide identifcation of the SUT superfamily in Camellia oleifera is lacking,and their biological function remains elusive.In this study,four SUT genes-designated Co SUT1-4-were identifed in C.oleifera through a genome-wide analysis and classifed into three subfamilies.We used a combination of cis-acting elements analysis,mRNA quantifcation,histochemical analysis,and heterologous transformation to evaluate the expression profiles and functions of these SUTs.A key finding is that CoSUT4,localized on the plasma membrane,is highly expressed in mature leaves and the early stage of seed development in C.oleifera.In vitro culture of C.oleifera seed revealed the responsiveness of CoSUT4 to various exogenous hormones such as ABA and GA.CoSUT4 was able to restore the growth of the yeast strain SUSY7/ura3(a sucrose transport-defcient mutant)on sucrose-containing media and specifcally contributed to sucrose translocation and tissue growth in CoSUT4-overexpressed apple calli.In situ hybridization identifed chalazal nucellus and transfer cells as the action sites of CoSUT4 at the maternal-flial interface mediating sucrose transportation in oil tea seeds.CoSUT4 overexpression in Arabidopsis thaliana atsuc4 mutant restored the growth and seed yield defciencies of the mutant,leading to an increase in flled seeds and oil content.Additionally,CoSUT4 overexpression enhanced the drought and salt stress tolerance by augmenting sugar content.Overall,these fndings provide valuable insights into the function of SUTs and present promising candidates for the genetic enhancement of seed production in C.oleifera.展开更多
Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield...Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield remains unclear.In this study,we identified five rice SUT genes(OsSUT1-OsSUT5)and examined their molecular characteristics and biological functions.OsSUT1,OsSUT2,and OsSUT4 were predominantly expressed in stems,while OsSUT3 and OsSUT5 showed higher expression in panicles.OsSUT1 and OsSUT4 are located on the plasma membrane,whereas OsSUT2,OsSUT3,and OsSUT5 are localized to the tonoplast.展开更多
[Objective] The aim was to determine the difference in sucrose content of different tartary buckwheat sources,so as to screen low-sucrose buckwheat materials.[Method] The sucrose contents of 35 tartary buckwheat mater...[Objective] The aim was to determine the difference in sucrose content of different tartary buckwheat sources,so as to screen low-sucrose buckwheat materials.[Method] The sucrose contents of 35 tartary buckwheat materials from different regions were measured.[Result] The sucrose content of the 35 tartary buckwheat materials varied from 0.007 7% to 0.208 9% with an average of 0.051 8%.There was some difference among the tartary buckwheat materials from different regions.The seeds from Hezhang in Guizhou had higher sucrose content,while the seeds from Nayong in Guizhou had lower sucrose content.[Conclusion] The content of sucrose was significantly low in tartary buckwheat,which could be popularized as a kind of low-sugar crop.展开更多
[Objective] The aim was to study the relationship between urcrose, zinc and the root system growth in rice. [Method] Changes of root system growth, ROS generation and root system proton export ability were analyzed in...[Objective] The aim was to study the relationship between urcrose, zinc and the root system growth in rice. [Method] Changes of root system growth, ROS generation and root system proton export ability were analyzed in rice （Oryza sativa L. cv Zhonghua No.11） treated with different concentrations of Zn （NO3）3 sucrose, com- bined sucrose and Zn （NO3）3 mannitol as well as mannitol plus Zn （NO3）2. [Result] The results showed that treatment with 1-3 mM Zn（NO3）2 resulted in significant increases in total root length /number and in accumulation of H202 and 02- but decreases in root system proton export ability. With the exception of shoot length, the length of primary, adventitious, and lateral roots, and the number of adventitious, and lateral roots on primary /adventitious roots were all influenced by different concentrations of sucrose. High concentrations of sucrose caused increases in H202 and O2-, starva- tion or high concentrations of sucrose reduced root system proton export ability after treating with or without Zn. However, at the same concentration of sucrose, different changes of these indicators were observed between Zn and non-Zn treatments. The regulation of root system growth induced by sucrose was marked different from that of mannitol at the same concentration of 5%, suggesting that these effects were caused by sugar signal but not by osmotic potential. [Conclusion] This study indicat- ed that both sucrose and Zn play important roles in the regulation of rice root system growth.展开更多
[Objective] The aim was to explore the effects of low night temperature treatment on sucrose synthesis ability of tomato leaves in seedling stage.[Method] Effects of low night temperature of 6 ℃（with 15 ℃ as contro...[Objective] The aim was to explore the effects of low night temperature treatment on sucrose synthesis ability of tomato leaves in seedling stage.[Method] Effects of low night temperature of 6 ℃（with 15 ℃ as control）on the sucrose synthesis ability of tomato leaves were studied after 1,3,5,7 d of treatment.[Result] The contents of fructose,glucose and sucrose in tomato leaves were higher than control at 6：00 during the treatment of low night temperature,but in contrast at 11：00.The contents of fructose,glucose and sucrose in tomato leaves of low night temperature treatment and control group at 6：00 were lower than that at 11：00.The content of starch in tomato leaves was higher than control at 6：00 and 11：00 during the treatment.And the activities of sugar metabolism enzymes of tomato were changed by the treatment of low night temperature.The activities of acid invertase（AI）and neutral invertase（NI）were higher than those of control,while the activities of sucrose synthase（SS）and sucrose phosphate synthase（SPS）were lower than control at 6：00 and 11：00 during the treatment.The activity of all the enzymes at 11：00 of low night temperature treatment plants treated for 1,3,5,7 d was higher than that at 6：00.Compared to 6：00,the invertase activity of the control plant decreased,while the synthetase activity increased.The activity of sucrose synthase（SS）was decreased for treated plants,and the increase amount of sucrose content in leaves was lower than that of control at 11：00 during the treatment,indicating that the sucrose synthesis ability of tomato leaves was decreased by the treatment of low night temperature.[Conclusion] The results in this study had provided theoretical basis for the environmental control of high-yield cultivation of tomato.展开更多
[Objective] To provide scientific bases for the propagation of Coleus blumei. [Method] With the healthy branches of potted Coleus blumei as experiment materials, effects of sucrose concentration on the rooting of Cole...[Objective] To provide scientific bases for the propagation of Coleus blumei. [Method] With the healthy branches of potted Coleus blumei as experiment materials, effects of sucrose concentration on the rooting of Coleus blumei cuttings as well as effects of sunlight on its growth were studied. [Results] Being compared with the CK (clear water), sucrose solution with low or high concentration will not promote the rooting of Coleus blumei cuttings; illumination will enhance the growth of Coleus blumei cuttings with roots; cutting treatment and management measures will greatly influence the rooting rate of Coleus blumei cuttings. [Conclusions] Sucrose solution will not promote the rooting of Coleus blumei cuttings; illumination will enhance the growth of Coleus blumei cuttings.展开更多
Although cryopreservation of embryos has been used in most terrestrial animals, the application of this technique has not been succeeded for aquatic animals. In this study, the authors investigate the effect of differ...Although cryopreservation of embryos has been used in most terrestrial animals, the application of this technique has not been succeeded for aquatic animals. In this study, the authors investigate the effect of different combinations of sucrose （SUC, C12H22OH） and cryoprotectants （CPAs） on the survival of the catfish embryos （Pangasidae hypophthalmus） at low temperatures （4, 0 and -20 ℃） for short-term storage. For this aim, embryos with somites and optic cups were exposed to different combinations of sucrose with methanol （SUC ＋ MeOH）, 1.2-propylene glycol （SUC ＋ PROH） and ethylene glycol （SUC ＋ EG） at four concentrations ratios： （1） 0.5 M SUC ＋ 0.5 M CPA; （2） 1 M SUC ＋ 0.5 M CPA; （3） 0.5 M SUC ＋ 1 M CPA; （4） 1 M SUC ＋ 1 M CPA for 40 min at 4, 0 and -20 ℃. Embryos kept in water at room temperature （RT）, 4, 0 and -20℃ were used as controls. The survival rate was expressed as a percentage of hatched embryos per total embryos treated. The results showed that the hatching rate declined significantly when embryos were stored in water at 0 ℃ and -20℃. For embryos at 0 ℃ storage, the highest survival rate （87.78%） was obtained with 1 M SUC ＋ 1 M MeOH combination while at -20 ℃, only embryos in the combined treatments of 0.5 M SUC ＋ 1 MEG and 0.5 M SUC ＋ 1 M PROH reached the hatching stage （40% and 83.33%, respectively）. In conclusion, the results showed that the catfish embryos are sensitive to sub-zero temperatures and the combined treatment of 0.5 M sucrose and 1 M propylene glycol can be used to protect catfish embryos from damages caused by low temperature （0 ℃ and -20 ℃）.展开更多
Sucrose synthases(SUS) are a family of enzymes that play pivotal roles in carbon partitioning, sink strength and plant development. A total of 11 SUS genes have been identified in the genome of Malus domestica(Md SUSs...Sucrose synthases(SUS) are a family of enzymes that play pivotal roles in carbon partitioning, sink strength and plant development. A total of 11 SUS genes have been identified in the genome of Malus domestica(Md SUSs), and phylogenetic analysis revealed that the Md SUS genes were divided into three groups, named as SUS I, SUS II and SUS III, respectively. The SUS I and SUS III groups included four homologs each, whereas the SUS II group contained three homologs. SUS genes in the same group showed similar structural characteristics, such as exon number, size and length distribution. After assessing four different tissues, Md SUS1 s and Md SUS2.1 showed the highest expression in fruit, whereas Md SUS2.2/2.3 and Md SUS3 s exhibit the highest expression in shoot tips. Most Md SUSs showed decreased expression during fruit development, similar to SUS enzyme activity, but both Md SUS2.1 and Md SUS1.4 displayed opposite expression profiles. These results suggest that different Md SUS genes might play distinct roles in the sink-source sugar cycle and sugar utilization in apple sink tissues.展开更多
Four irrigation treatments were designed with 2,4,6 and 8 d intervals to irrigate, respectively. Watering was stopped when the reading of the moisture tension sensor reached zero. The results indicated that glucose an...Four irrigation treatments were designed with 2,4,6 and 8 d intervals to irrigate, respectively. Watering was stopped when the reading of the moisture tension sensor reached zero. The results indicated that glucose and fructose content of tomato's fruit were increased but sucrose content was decreased with fruit growth and development. In different stages, carbohydrate content of tomato fruit in the treatment 3 was the highest, in the treatment 2 was higher, and in the other treatments was the lowest. SS(sucrose synthase)activity was decreased but SPS(sucrose phosphate synthase)activity was increased with development of tomato. SS and SPS activity were increased but acid invertase and neutral invertase activity of ripe stage were decreased under deficit irrigation. Glucose and fructose content were increased in leaves of tomato under water deficit. Soluble sugars, organic acid and the ratio of sugar/acid in tomato fruits were increased and dry matter accumulation of plant was enhanced under water deficit. But the growth of fruits upside the plant and its dry matter accumulation were badly affected under water stress.展开更多
This work evaluated the effects of sodium ion concentration, ranging from 0 to 16000mg·L^-1（Na^＋）, on the conversion of sucrose to hydrogen by a high-activity anaerobic hydrogen-producing granular sludge. At ...This work evaluated the effects of sodium ion concentration, ranging from 0 to 16000mg·L^-1（Na^＋）, on the conversion of sucrose to hydrogen by a high-activity anaerobic hydrogen-producing granular sludge. At the optimum sodium ion concentration [1000-2000mg·L^-1（Na^＋）] for hydrogen production at 37℃, the maximum sucrose degradation rate, the specific hydrogen production yield and the specific hydrogen production rate were 393.6-413.1mg·L^-1.h^-1, 28.04-28.97ml·g^-1, 7.52-7.83ml·g^-1.h^-1, respectively. The specific production yields of propionate, butyrate and valerate decreased with increasing sodium ion concentration, whereas the specific acetate production yield increased, meanwhile the specific production yields of ethanol and caproate were less than 55.3 and 12.6mg·g^-1, respectively. The hybrid fermentation composition gradually developed from acetate, propionate and butyrate to acetate with the increase in sodium ion concentration.展开更多
Sucrose phosphate synthase(SPS)is a rate-limiting enzyme that works in conjunction with sucrose-6-phosphate phosphatase(SPP)for sucrose synthesis,and it plays an essential role in energy provisioning during growth and...Sucrose phosphate synthase(SPS)is a rate-limiting enzyme that works in conjunction with sucrose-6-phosphate phosphatase(SPP)for sucrose synthesis,and it plays an essential role in energy provisioning during growth and development in plants as well as improving fruit quality.However,studies on the systematic analysis and evolutionary pattern of the SPS gene family in apple are still lacking.In the present study,a total of seven MdSPS and four MdSPP genes were identified from the Malus domestica genome GDDH13 v1.1.The gene structures and their promoter cis-elements,protein conserved motifs,subcellular localizations,physiological functions and biochemical properties were analyzed.A chromosomal location and gene-duplication analysis demonstrated that whole-genome duplication(WGD)and segmental duplication played vital roles in MdSPS gene family expansion.The Ka/Ks ratio of pairwise MdSPS genes indicated that the members of this family have undergone strong purifying selection during domestication.Furthermore,three SPS gene subfamilies were classified based on phylogenetic relationships,and old gene duplications and significantly divergent evolutionary rates were observed among the SPS gene subfamilies.In addition,a major gene related to sucrose accumulation(MdSPSA2.3)was identified according to the highly consistent trends in the changes of its expression in four apple varieties(‘Golden Delicious’,‘Fuji’,‘Qinguan’and‘Honeycrisp’)and the correlation between gene expression and soluble sugar content during fruit development.Furthermore,the virus-induced silencing of MdSPSA2.3 confirmed its function in sucrose accumulation in apple fruit.The present study lays a theoretical foundation for better clarifying the biological functions of the MdSPS genes during apple fruit development.展开更多
Experimental data on the solubility of D-glucose,D-fructose and sucrose in the mixed solvents com-posed of water and ethanol from 273.2 to 293.2 K were determined.The solubility of D-glucose,D-fructose and sucrose dec...Experimental data on the solubility of D-glucose,D-fructose and sucrose in the mixed solvents com-posed of water and ethanol from 273.2 to 293.2 K were determined.The solubility of D-glucose,D-fructose and sucrose decreased as the ethanol content increased in the mixed solvent.The solubility of D-glucose,D-fructose and sucrose decreased with decreasing equilibrium temperature.The modified UNIQUAC model,S-UNIFAC model and mS-UNIFAC model were applied to predict the solid-liquid equilibria.The prediction results were compared and discussed.Better prediction accuracy was generated using the modified UNIQUAC model.展开更多
AIM： To investigate the usefulness of sucrose permeability test using serum in the diagnosis of gastric diseases, with special reference to early gastric cancer （EGC）. METHODS： A total of 63 subjects, including 11...AIM： To investigate the usefulness of sucrose permeability test using serum in the diagnosis of gastric diseases, with special reference to early gastric cancer （EGC）. METHODS： A total of 63 subjects, including 11 patients with gastric ulcer, 20 patients with gastric cancer （13, early; 7, advanced） and 32 healthy controls, were studied. Blood and urine samples were collected repeatedly for 5 h before and after the sucrose loading. Sucrose levels were measured by a newly developed RESULTS： Serum sucrose levels started to increase 15 min after loading, and peaked at 60 min in the gastric disease groups. The levels for gastric ulcer, EGC and advanced gastric cancer （AGC） at 60 min were significantly higher than that in the healthy controls （26.9±2.4, 34.4±5.0, and 71.8±15.6 vs 7.9±0.7 mol/L, respectively, P〈0.01）. The cut-off level set at 15.4 mol/L （60 min） offered the best distinction between EGC patients and healthy controls; and the sensitivity and specificity were 92.3% and 93.8%, respectively, while those of the urine method were 76.9% and 93.8%, respectively. CONCLUSIONS： The gastric permeability test using serum is reliable for the detection of EGC, and this test can provide results much earlier than the conventional urine method. This test may offer a useful alternative to more invasive tests for EGC.展开更多
Dynamic changes of sucrose, fructose, glucose contents and differences in activities of sucrose synthase, vacuolar invertase, and cell wall bound invertase in rice grain after flowering stage were studied under natura...Dynamic changes of sucrose, fructose, glucose contents and differences in activities of sucrose synthase, vacuolar invertase, and cell wall bound invertase in rice grain after flowering stage were studied under natural and high temperatures by using two japonica rice varieties Koshihikari and Sasanishiki. In rice grains, the sucrose synthase activity was higher than that of invertase, which was significantly correlated with starch accumulation rate, indicating that the sucrose synthase played an important role in sucrose degradation and starch synthesis. Under high temperature, the significant increase in grain sucrose content without any increase in fructose and glucose contents, suggested that the high temperature treatment enhanced sucrose accumulation, while diminished sucrose degradation in rice grains. Compared with the control plants, the decrease in activities of sucrose synthase, vacuolar invertase, and cell wall bound invertase with high temperature treated plants indicated that the deceleration of sucrose-degradation was related to the decrease in activities of sucrose synthase and invertase.展开更多
In higher plants,sugars(mainly sucrose)are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and devel-opment.Sucrose transp...In higher plants,sugars(mainly sucrose)are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and devel-opment.Sucrose transporters,or sucrose carriers(SUCs),play an important role in the long-distance transportation of sucrose from source organs to sink organs,thereby affecting crop yield and quality.The identification,characterization,and molecular function analysis of sucrose transporter genes have been reported for monocot and dicot plants.However,no relevant study has been reported on sucrose transporter genes in Brassica rapa vat.rapa,a cruciferous root crop used mainly as vegetables and fodder.We identified and cloned 12 sucrose transporter genes from turnips,named BrrSUC1.1 to BrrSUCB.2 according to the SUC gene sequences of B.rapa pekinensis.We constructed a phylogenetic tree and analyzed conserved motifs for all 12 sucrose transporter genes identified.Real-time quantitative poly-merase chain reaction was conducted to understand the expression levels of SUC genes in different tissues and developmental phases of the turnip.These findings add to our understanding of the genetics and physiology of sugar transport during taproot formation in turnips.展开更多
Some synthetic sucrose esters （SE） are a relatively new class of insecticidal compounds produced by reacting sugars with fatty acids, which are safe for the environment. Especially, sucrose esters composed of C6-C12...Some synthetic sucrose esters （SE） are a relatively new class of insecticidal compounds produced by reacting sugars with fatty acids, which are safe for the environment. Especially, sucrose esters composed of C6-C12 fatty acids have desirable insecticidal properties against many soft-bodied arthropod pests. In our study, sucrose octanoate which has the highest activity against a range of arthropod species was synthesized by a trans-esterification method and proved its insecticidal property. Under the condition of a homogeneous liquid, sucrose octanoate was prepared by reacting ethyl octanoate with sucrose at reduced pressure; the yield was 79.11%. Sucrose octanoate synthesized was identified and its property analyzed by IR, TLC and spectrophotometric analysis. It was shown that the ratio of monoester to polyester in sucrose octanoate was 1.48：1. The insecticidal activity of the synthetic sucrose octanoate was evaluated at a concentration of 4 and 8 mg.mL^-1. The mortality of first-instar larvae ofLymantria dispar from its contact toxicity was 72.5% after 36 hours, the revision insect reduced rate of Aphis glycines reached above 80% at 4 and 8 mg.mL^-1 after being treated for 5 days. Since the SE products are nontoxic to humans and higher animals, fully biodegradable and hydrolyzed to readily metabolizable sucrose and fatty acid, they are not harmful to crops and appear to be good insecticide candidates.展开更多
A robust voltammetric method has been developed and validated for the determination of Fe（Ⅱ） and Fe（Ⅲ） in pharmaceutical iron polysaccharidic complexes. Undesirable low molecular weight iron complexes, at concen...A robust voltammetric method has been developed and validated for the determination of Fe（Ⅱ） and Fe（Ⅲ） in pharmaceutical iron polysaccharidic complexes. Undesirable low molecular weight iron complexes, at concentration about 3% in the pharmaceutical formulation, can be easily determined with good accuracy and precision. This methodology can be proposed as a viable, environmentally sustainable substitute for the conventional Normal Pulse Polarographic method in US Pharmacopeia, with better analytical figures of merit, and reduced Hg consumption. A deeper insight in Fe（Ⅱ） and Fe（Ⅲ） composition can be gained by the combined use of a new potentiometric technique after chemical decomposition of the complex.展开更多
The analysis of sucrose esters with long acyl chain by improved high performance liquid chromatographic method with evaporative light scattering detection （HPLC-ELSD） and electrospray ionization mass spectrum （ESI...The analysis of sucrose esters with long acyl chain by improved high performance liquid chromatographic method with evaporative light scattering detection （HPLC-ELSD） and electrospray ionization mass spectrum （ESI-MS） is investigated. The improved HPLC-ELSD method for the separation and quantitation of commercial and synthesized sucrose esters is described. Samples are analyzed by means of a reversed-phase （RP） HPLC using a Hypersil C8 column （250 mm× 4.6 mm, 5 μm particle size） with methanol-tetrahydrofuran （vo）ume ratio of 90 ： 10） and water under gradientcondition as the mobile phase, in which the flow rate is 1.0 ml·min^-1 and the column temperature is set at 40℃. This procedure provides a complete separation and determination ot monoester, diester, triester and higher esters with different acyl chain lengths in each fraction by a single run, in combination with the ESI-MS technology. With this method, it is possible to determine the approximate compositions of monoto polyesters in one analysis and quantitate pure positional isomers precisely using an external standard method. It is found that the method of ESI-MS coupling with HPLC system for the analysis of sucrose esters is straight forward, rapid and inexpensive, and can be readily applied in synthesis, purification and structure studies.展开更多
The formation of mature and fertile pollen grains, taking place inside the anther, depends on supply of assimilates, in the form of sucrose, provided mainly by the leaves. Data is limited, however, with respect to the...The formation of mature and fertile pollen grains, taking place inside the anther, depends on supply of assimilates, in the form of sucrose, provided mainly by the leaves. Data is limited, however, with respect to the understanding of sucrose metabolism in microspores and the supporting tissues. The aims of the present work were to 1) follow the changes in total and relative concentrations of sucrose, glucose, fructose and starch in the stamen parts and microspores up until anthesis, 2) follow the activities of sucrose-metabolism-related enzymes, in the anther walls fraction and microspores of the crop plant tomato. Sucrose was found to be partially cleaved in the filament, decreasing by more than twofold in the anther wall layers and the locular fluid, and to accumulate in the mature pollen grains, constituting 80% of total soluble sugars. Thus, sucrose was both the starting sugar, supporting microspore development, and the main carbohydrate accumulated at the end of the pollen-development program. The major invertase found to be active in both the anther wall layers and in maturing microspores was cell-wall-bound invertase. High fructokinase 2 and sucrose phosphate synthase activities during pollen maturation coincided with sucrose accumulation. The potential importance of sucrose accumulation during pollen dehydration phase and germination is discussed.展开更多
基金supported by the Key Research and Development Program of Shaanxi,China(2021NY-083)the National Natural Science Foundation of China(31871567)。
文摘The trade-off between yield and environmental effects caused by nitrogen fertilizer application is an important issue in wheat production.A reduction in fertile florets is one of the main reasons for the lower yields under low nitrogen application rates.Brassinosteroids(BRs)have been found to play a role in nitrogen-induced rice spikelet degeneration.However,whether BRs play a role in wheat floret development and the mechanisms involved are not clear.Therefore,a nitrogen gradient experiment and exogenous spraying experiment were conducted to investigate the role and mechanism of BRs in wheat floret development under low nitrogen stress.The results showed that as the nitrogen application decreased,the endogenous BRs content of the spikes decreased,photosynthesis weakened,and total carbon,soluble sugar and starch in the spikes decreased,leading to a reduction in the number of fertile florets.Under low nitrogen stress,exogenous spraying of 24-epibrassinolide promoted photosynthesis,and stimulated stem fructan hydrolysis and the utilization and storage of sucrose in spikes,which directed more carbohydrates to the spikes and increased the number of fertile florets.In conclusion,BRs mediate the effects of nitrogen fertilizer on wheat floret development,and under low nitrogen stress,foliar spraying of 24-epibrassinolide promotes the flow of carbohydrates from the stem to the spikes,alleviating wheat floret degeneration.
基金supported by the National Natural Science Foundation of China(32071798)5·5 Engineering Research&Innovation Team Project of Beijing Forestry University,China(BLRC2023B08)。
文摘Sucrose transporters(SUTs)play a crucial role in carbon allocation from the source leaf to the sink end,and the function of SUTs varies among family members.However,the genome-wide identifcation of the SUT superfamily in Camellia oleifera is lacking,and their biological function remains elusive.In this study,four SUT genes-designated Co SUT1-4-were identifed in C.oleifera through a genome-wide analysis and classifed into three subfamilies.We used a combination of cis-acting elements analysis,mRNA quantifcation,histochemical analysis,and heterologous transformation to evaluate the expression profiles and functions of these SUTs.A key finding is that CoSUT4,localized on the plasma membrane,is highly expressed in mature leaves and the early stage of seed development in C.oleifera.In vitro culture of C.oleifera seed revealed the responsiveness of CoSUT4 to various exogenous hormones such as ABA and GA.CoSUT4 was able to restore the growth of the yeast strain SUSY7/ura3(a sucrose transport-defcient mutant)on sucrose-containing media and specifcally contributed to sucrose translocation and tissue growth in CoSUT4-overexpressed apple calli.In situ hybridization identifed chalazal nucellus and transfer cells as the action sites of CoSUT4 at the maternal-flial interface mediating sucrose transportation in oil tea seeds.CoSUT4 overexpression in Arabidopsis thaliana atsuc4 mutant restored the growth and seed yield defciencies of the mutant,leading to an increase in flled seeds and oil content.Additionally,CoSUT4 overexpression enhanced the drought and salt stress tolerance by augmenting sugar content.Overall,these fndings provide valuable insights into the function of SUTs and present promising candidates for the genetic enhancement of seed production in C.oleifera.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFF1000500)the National Natural Science Foundation of China(Grant Nos.32372099,32172080,and 32188102)the Innovation Program of the Chinese Academy of Agricultural Sciences,China(Grant No.CAAS-CSCB-202402).
文摘Sucrose transporters(SUTs)contain multiple transmembrane domains that mediate sucrose transport and provide energy for plant growth and development.However,the role of OsSUTs in regulating rice quality and grain yield remains unclear.In this study,we identified five rice SUT genes(OsSUT1-OsSUT5)and examined their molecular characteristics and biological functions.OsSUT1,OsSUT2,and OsSUT4 were predominantly expressed in stems,while OsSUT3 and OsSUT5 showed higher expression in panicles.OsSUT1 and OsSUT4 are located on the plasma membrane,whereas OsSUT2,OsSUT3,and OsSUT5 are localized to the tonoplast.
基金Supported by the Science and Technology Foundation of Guizhou Province[J(2009)2108]Agricultural Research Project of Guizhou Province[NY(2010)3094]Special Program of Plant and Animal Breeding of Guizhou Province[No.(2010)023]~~
文摘[Objective] The aim was to determine the difference in sucrose content of different tartary buckwheat sources,so as to screen low-sucrose buckwheat materials.[Method] The sucrose contents of 35 tartary buckwheat materials from different regions were measured.[Result] The sucrose content of the 35 tartary buckwheat materials varied from 0.007 7% to 0.208 9% with an average of 0.051 8%.There was some difference among the tartary buckwheat materials from different regions.The seeds from Hezhang in Guizhou had higher sucrose content,while the seeds from Nayong in Guizhou had lower sucrose content.[Conclusion] The content of sucrose was significantly low in tartary buckwheat,which could be popularized as a kind of low-sugar crop.
基金Supported by the National Natural Science Foundation of China(30671126)the Science and Technology Development Planning Project of Zibo City(2009)~~
文摘[Objective] The aim was to study the relationship between urcrose, zinc and the root system growth in rice. [Method] Changes of root system growth, ROS generation and root system proton export ability were analyzed in rice （Oryza sativa L. cv Zhonghua No.11） treated with different concentrations of Zn （NO3）3 sucrose, com- bined sucrose and Zn （NO3）3 mannitol as well as mannitol plus Zn （NO3）2. [Result] The results showed that treatment with 1-3 mM Zn（NO3）2 resulted in significant increases in total root length /number and in accumulation of H202 and 02- but decreases in root system proton export ability. With the exception of shoot length, the length of primary, adventitious, and lateral roots, and the number of adventitious, and lateral roots on primary /adventitious roots were all influenced by different concentrations of sucrose. High concentrations of sucrose caused increases in H202 and O2-, starva- tion or high concentrations of sucrose reduced root system proton export ability after treating with or without Zn. However, at the same concentration of sucrose, different changes of these indicators were observed between Zn and non-Zn treatments. The regulation of root system growth induced by sucrose was marked different from that of mannitol at the same concentration of 5%, suggesting that these effects were caused by sugar signal but not by osmotic potential. [Conclusion] This study indicat- ed that both sucrose and Zn play important roles in the regulation of rice root system growth.
基金Supported by National Natural Science Foundation of China(30170640)~~
文摘[Objective] The aim was to explore the effects of low night temperature treatment on sucrose synthesis ability of tomato leaves in seedling stage.[Method] Effects of low night temperature of 6 ℃（with 15 ℃ as control）on the sucrose synthesis ability of tomato leaves were studied after 1,3,5,7 d of treatment.[Result] The contents of fructose,glucose and sucrose in tomato leaves were higher than control at 6：00 during the treatment of low night temperature,but in contrast at 11：00.The contents of fructose,glucose and sucrose in tomato leaves of low night temperature treatment and control group at 6：00 were lower than that at 11：00.The content of starch in tomato leaves was higher than control at 6：00 and 11：00 during the treatment.And the activities of sugar metabolism enzymes of tomato were changed by the treatment of low night temperature.The activities of acid invertase（AI）and neutral invertase（NI）were higher than those of control,while the activities of sucrose synthase（SS）and sucrose phosphate synthase（SPS）were lower than control at 6：00 and 11：00 during the treatment.The activity of all the enzymes at 11：00 of low night temperature treatment plants treated for 1,3,5,7 d was higher than that at 6：00.Compared to 6：00,the invertase activity of the control plant decreased,while the synthetase activity increased.The activity of sucrose synthase（SS）was decreased for treated plants,and the increase amount of sucrose content in leaves was lower than that of control at 11：00 during the treatment,indicating that the sucrose synthesis ability of tomato leaves was decreased by the treatment of low night temperature.[Conclusion] The results in this study had provided theoretical basis for the environmental control of high-yield cultivation of tomato.
文摘[Objective] To provide scientific bases for the propagation of Coleus blumei. [Method] With the healthy branches of potted Coleus blumei as experiment materials, effects of sucrose concentration on the rooting of Coleus blumei cuttings as well as effects of sunlight on its growth were studied. [Results] Being compared with the CK (clear water), sucrose solution with low or high concentration will not promote the rooting of Coleus blumei cuttings; illumination will enhance the growth of Coleus blumei cuttings with roots; cutting treatment and management measures will greatly influence the rooting rate of Coleus blumei cuttings. [Conclusions] Sucrose solution will not promote the rooting of Coleus blumei cuttings; illumination will enhance the growth of Coleus blumei cuttings.
文摘Although cryopreservation of embryos has been used in most terrestrial animals, the application of this technique has not been succeeded for aquatic animals. In this study, the authors investigate the effect of different combinations of sucrose （SUC, C12H22OH） and cryoprotectants （CPAs） on the survival of the catfish embryos （Pangasidae hypophthalmus） at low temperatures （4, 0 and -20 ℃） for short-term storage. For this aim, embryos with somites and optic cups were exposed to different combinations of sucrose with methanol （SUC ＋ MeOH）, 1.2-propylene glycol （SUC ＋ PROH） and ethylene glycol （SUC ＋ EG） at four concentrations ratios： （1） 0.5 M SUC ＋ 0.5 M CPA; （2） 1 M SUC ＋ 0.5 M CPA; （3） 0.5 M SUC ＋ 1 M CPA; （4） 1 M SUC ＋ 1 M CPA for 40 min at 4, 0 and -20 ℃. Embryos kept in water at room temperature （RT）, 4, 0 and -20℃ were used as controls. The survival rate was expressed as a percentage of hatched embryos per total embryos treated. The results showed that the hatching rate declined significantly when embryos were stored in water at 0 ℃ and -20℃. For embryos at 0 ℃ storage, the highest survival rate （87.78%） was obtained with 1 M SUC ＋ 1 M MeOH combination while at -20 ℃, only embryos in the combined treatments of 0.5 M SUC ＋ 1 MEG and 0.5 M SUC ＋ 1 M PROH reached the hatching stage （40% and 83.33%, respectively）. In conclusion, the results showed that the catfish embryos are sensitive to sub-zero temperatures and the combined treatment of 0.5 M sucrose and 1 M propylene glycol can be used to protect catfish embryos from damages caused by low temperature （0 ℃ and -20 ℃）.
基金supported in part by the National Natural Science Foundation of China (31372038)the Natural Basic Research Plan in Shaanxi Province of China (2015JQ3082)
文摘Sucrose synthases(SUS) are a family of enzymes that play pivotal roles in carbon partitioning, sink strength and plant development. A total of 11 SUS genes have been identified in the genome of Malus domestica(Md SUSs), and phylogenetic analysis revealed that the Md SUS genes were divided into three groups, named as SUS I, SUS II and SUS III, respectively. The SUS I and SUS III groups included four homologs each, whereas the SUS II group contained three homologs. SUS genes in the same group showed similar structural characteristics, such as exon number, size and length distribution. After assessing four different tissues, Md SUS1 s and Md SUS2.1 showed the highest expression in fruit, whereas Md SUS2.2/2.3 and Md SUS3 s exhibit the highest expression in shoot tips. Most Md SUSs showed decreased expression during fruit development, similar to SUS enzyme activity, but both Md SUS2.1 and Md SUS1.4 displayed opposite expression profiles. These results suggest that different Md SUS genes might play distinct roles in the sink-source sugar cycle and sugar utilization in apple sink tissues.
基金supported by the National Natural Science Fundation of China(30170640)Natural Science Fund of Liaoning Provice,China(20022080).
文摘Four irrigation treatments were designed with 2,4,6 and 8 d intervals to irrigate, respectively. Watering was stopped when the reading of the moisture tension sensor reached zero. The results indicated that glucose and fructose content of tomato's fruit were increased but sucrose content was decreased with fruit growth and development. In different stages, carbohydrate content of tomato fruit in the treatment 3 was the highest, in the treatment 2 was higher, and in the other treatments was the lowest. SS(sucrose synthase)activity was decreased but SPS(sucrose phosphate synthase)activity was increased with development of tomato. SS and SPS activity were increased but acid invertase and neutral invertase activity of ripe stage were decreased under deficit irrigation. Glucose and fructose content were increased in leaves of tomato under water deficit. Soluble sugars, organic acid and the ratio of sugar/acid in tomato fruits were increased and dry matter accumulation of plant was enhanced under water deficit. But the growth of fruits upside the plant and its dry matter accumulation were badly affected under water stress.
基金Supported by the National Natural Science Foundation of China （No.20122203）.
文摘This work evaluated the effects of sodium ion concentration, ranging from 0 to 16000mg·L^-1（Na^＋）, on the conversion of sucrose to hydrogen by a high-activity anaerobic hydrogen-producing granular sludge. At the optimum sodium ion concentration [1000-2000mg·L^-1（Na^＋）] for hydrogen production at 37℃, the maximum sucrose degradation rate, the specific hydrogen production yield and the specific hydrogen production rate were 393.6-413.1mg·L^-1.h^-1, 28.04-28.97ml·g^-1, 7.52-7.83ml·g^-1.h^-1, respectively. The specific production yields of propionate, butyrate and valerate decreased with increasing sodium ion concentration, whereas the specific acetate production yield increased, meanwhile the specific production yields of ethanol and caproate were less than 55.3 and 12.6mg·g^-1, respectively. The hybrid fermentation composition gradually developed from acetate, propionate and butyrate to acetate with the increase in sodium ion concentration.
基金supported by the National Natural Science Foundation of China (32172521)the Excellent Youth Science Foundation of Heilongjiang Province,China (YQ2023C006)+1 种基金the Talent Introduction Program of Northeast Agricultural University of Chinathe Collaborative Innovation System of the Agricultural Bio-economy in Heilongjiang Province,China
文摘Sucrose phosphate synthase(SPS)is a rate-limiting enzyme that works in conjunction with sucrose-6-phosphate phosphatase(SPP)for sucrose synthesis,and it plays an essential role in energy provisioning during growth and development in plants as well as improving fruit quality.However,studies on the systematic analysis and evolutionary pattern of the SPS gene family in apple are still lacking.In the present study,a total of seven MdSPS and four MdSPP genes were identified from the Malus domestica genome GDDH13 v1.1.The gene structures and their promoter cis-elements,protein conserved motifs,subcellular localizations,physiological functions and biochemical properties were analyzed.A chromosomal location and gene-duplication analysis demonstrated that whole-genome duplication(WGD)and segmental duplication played vital roles in MdSPS gene family expansion.The Ka/Ks ratio of pairwise MdSPS genes indicated that the members of this family have undergone strong purifying selection during domestication.Furthermore,three SPS gene subfamilies were classified based on phylogenetic relationships,and old gene duplications and significantly divergent evolutionary rates were observed among the SPS gene subfamilies.In addition,a major gene related to sucrose accumulation(MdSPSA2.3)was identified according to the highly consistent trends in the changes of its expression in four apple varieties(‘Golden Delicious’,‘Fuji’,‘Qinguan’and‘Honeycrisp’)and the correlation between gene expression and soluble sugar content during fruit development.Furthermore,the virus-induced silencing of MdSPSA2.3 confirmed its function in sucrose accumulation in apple fruit.The present study lays a theoretical foundation for better clarifying the biological functions of the MdSPS genes during apple fruit development.
基金Supported by the National Science and Technology Major Project （2009ZX09313-036） the China Postdoctoral Science Foundation （20090461360） the Zhejiang Provincial Education Department Projects （Y200907556）
文摘Experimental data on the solubility of D-glucose,D-fructose and sucrose in the mixed solvents com-posed of water and ethanol from 273.2 to 293.2 K were determined.The solubility of D-glucose,D-fructose and sucrose decreased as the ethanol content increased in the mixed solvent.The solubility of D-glucose,D-fructose and sucrose decreased with decreasing equilibrium temperature.The modified UNIQUAC model,S-UNIFAC model and mS-UNIFAC model were applied to predict the solid-liquid equilibria.The prediction results were compared and discussed.Better prediction accuracy was generated using the modified UNIQUAC model.
文摘AIM： To investigate the usefulness of sucrose permeability test using serum in the diagnosis of gastric diseases, with special reference to early gastric cancer （EGC）. METHODS： A total of 63 subjects, including 11 patients with gastric ulcer, 20 patients with gastric cancer （13, early; 7, advanced） and 32 healthy controls, were studied. Blood and urine samples were collected repeatedly for 5 h before and after the sucrose loading. Sucrose levels were measured by a newly developed RESULTS： Serum sucrose levels started to increase 15 min after loading, and peaked at 60 min in the gastric disease groups. The levels for gastric ulcer, EGC and advanced gastric cancer （AGC） at 60 min were significantly higher than that in the healthy controls （26.9±2.4, 34.4±5.0, and 71.8±15.6 vs 7.9±0.7 mol/L, respectively, P〈0.01）. The cut-off level set at 15.4 mol/L （60 min） offered the best distinction between EGC patients and healthy controls; and the sensitivity and specificity were 92.3% and 93.8%, respectively, while those of the urine method were 76.9% and 93.8%, respectively. CONCLUSIONS： The gastric permeability test using serum is reliable for the detection of EGC, and this test can provide results much earlier than the conventional urine method. This test may offer a useful alternative to more invasive tests for EGC.
文摘Dynamic changes of sucrose, fructose, glucose contents and differences in activities of sucrose synthase, vacuolar invertase, and cell wall bound invertase in rice grain after flowering stage were studied under natural and high temperatures by using two japonica rice varieties Koshihikari and Sasanishiki. In rice grains, the sucrose synthase activity was higher than that of invertase, which was significantly correlated with starch accumulation rate, indicating that the sucrose synthase played an important role in sucrose degradation and starch synthesis. Under high temperature, the significant increase in grain sucrose content without any increase in fructose and glucose contents, suggested that the high temperature treatment enhanced sucrose accumulation, while diminished sucrose degradation in rice grains. Compared with the control plants, the decrease in activities of sucrose synthase, vacuolar invertase, and cell wall bound invertase with high temperature treated plants indicated that the deceleration of sucrose-degradation was related to the decrease in activities of sucrose synthase and invertase.
基金supported by Major Program of National Natural Science Foundation of China (31590820,31590823)
文摘In higher plants,sugars(mainly sucrose)are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and devel-opment.Sucrose transporters,or sucrose carriers(SUCs),play an important role in the long-distance transportation of sucrose from source organs to sink organs,thereby affecting crop yield and quality.The identification,characterization,and molecular function analysis of sucrose transporter genes have been reported for monocot and dicot plants.However,no relevant study has been reported on sucrose transporter genes in Brassica rapa vat.rapa,a cruciferous root crop used mainly as vegetables and fodder.We identified and cloned 12 sucrose transporter genes from turnips,named BrrSUC1.1 to BrrSUCB.2 according to the SUC gene sequences of B.rapa pekinensis.We constructed a phylogenetic tree and analyzed conserved motifs for all 12 sucrose transporter genes identified.Real-time quantitative poly-merase chain reaction was conducted to understand the expression levels of SUC genes in different tissues and developmental phases of the turnip.These findings add to our understanding of the genetics and physiology of sugar transport during taproot formation in turnips.
文摘Some synthetic sucrose esters （SE） are a relatively new class of insecticidal compounds produced by reacting sugars with fatty acids, which are safe for the environment. Especially, sucrose esters composed of C6-C12 fatty acids have desirable insecticidal properties against many soft-bodied arthropod pests. In our study, sucrose octanoate which has the highest activity against a range of arthropod species was synthesized by a trans-esterification method and proved its insecticidal property. Under the condition of a homogeneous liquid, sucrose octanoate was prepared by reacting ethyl octanoate with sucrose at reduced pressure; the yield was 79.11%. Sucrose octanoate synthesized was identified and its property analyzed by IR, TLC and spectrophotometric analysis. It was shown that the ratio of monoester to polyester in sucrose octanoate was 1.48：1. The insecticidal activity of the synthetic sucrose octanoate was evaluated at a concentration of 4 and 8 mg.mL^-1. The mortality of first-instar larvae ofLymantria dispar from its contact toxicity was 72.5% after 36 hours, the revision insect reduced rate of Aphis glycines reached above 80% at 4 and 8 mg.mL^-1 after being treated for 5 days. Since the SE products are nontoxic to humans and higher animals, fully biodegradable and hydrolyzed to readily metabolizable sucrose and fatty acid, they are not harmful to crops and appear to be good insecticide candidates.
基金supported by FAR, Fondo Ateneoper la Ricerca Universitá di Pavia,Italy
文摘A robust voltammetric method has been developed and validated for the determination of Fe（Ⅱ） and Fe（Ⅲ） in pharmaceutical iron polysaccharidic complexes. Undesirable low molecular weight iron complexes, at concentration about 3% in the pharmaceutical formulation, can be easily determined with good accuracy and precision. This methodology can be proposed as a viable, environmentally sustainable substitute for the conventional Normal Pulse Polarographic method in US Pharmacopeia, with better analytical figures of merit, and reduced Hg consumption. A deeper insight in Fe（Ⅱ） and Fe（Ⅲ） composition can be gained by the combined use of a new potentiometric technique after chemical decomposition of the complex.
基金Supported by the National Natural Science Foundation of China （20906052）, the Science Foundation of Nantong City Municipality （K2007011, K2008023）, the Science Foundation of Nantong University （08R08） and the University Science Research Project of Jiangsu Province （09KJB530008）.
文摘The analysis of sucrose esters with long acyl chain by improved high performance liquid chromatographic method with evaporative light scattering detection （HPLC-ELSD） and electrospray ionization mass spectrum （ESI-MS） is investigated. The improved HPLC-ELSD method for the separation and quantitation of commercial and synthesized sucrose esters is described. Samples are analyzed by means of a reversed-phase （RP） HPLC using a Hypersil C8 column （250 mm× 4.6 mm, 5 μm particle size） with methanol-tetrahydrofuran （vo）ume ratio of 90 ： 10） and water under gradientcondition as the mobile phase, in which the flow rate is 1.0 ml·min^-1 and the column temperature is set at 40℃. This procedure provides a complete separation and determination ot monoester, diester, triester and higher esters with different acyl chain lengths in each fraction by a single run, in combination with the ESI-MS technology. With this method, it is possible to determine the approximate compositions of monoto polyesters in one analysis and quantitate pure positional isomers precisely using an external standard method. It is found that the method of ESI-MS coupling with HPLC system for the analysis of sucrose esters is straight forward, rapid and inexpensive, and can be readily applied in synthesis, purification and structure studies.
文摘The formation of mature and fertile pollen grains, taking place inside the anther, depends on supply of assimilates, in the form of sucrose, provided mainly by the leaves. Data is limited, however, with respect to the understanding of sucrose metabolism in microspores and the supporting tissues. The aims of the present work were to 1) follow the changes in total and relative concentrations of sucrose, glucose, fructose and starch in the stamen parts and microspores up until anthesis, 2) follow the activities of sucrose-metabolism-related enzymes, in the anther walls fraction and microspores of the crop plant tomato. Sucrose was found to be partially cleaved in the filament, decreasing by more than twofold in the anther wall layers and the locular fluid, and to accumulate in the mature pollen grains, constituting 80% of total soluble sugars. Thus, sucrose was both the starting sugar, supporting microspore development, and the main carbohydrate accumulated at the end of the pollen-development program. The major invertase found to be active in both the anther wall layers and in maturing microspores was cell-wall-bound invertase. High fructokinase 2 and sucrose phosphate synthase activities during pollen maturation coincided with sucrose accumulation. The potential importance of sucrose accumulation during pollen dehydration phase and germination is discussed.
