In this study,we investigated improving the performance of a layered double hydroxide(LDH)for the adsorption of As(III)and As(V)by controlling the morphology of LDH crystals.The LDH was synthesized via a simple coprec...In this study,we investigated improving the performance of a layered double hydroxide(LDH)for the adsorption of As(III)and As(V)by controlling the morphology of LDH crystals.The LDH was synthesized via a simple coprecipitation method using barely soluble MgO as a precursor and succinic acid(SA)as a morphological control agent.Doping the LDH crystals with carboxylate ions(RCOO−)derived from SA caused the crystals to develop in a radial direction.This changed the pore characteristics and increased the density of active surface sites.Subsequently,SA/MgFe-LDH showed excellent affinity for As(III)and As(V)with maximum sorption densities of 2.42 and 1.60 mmol/g,respectively.By comparison,the pristine MgFe-LDH had sorption capacities of 1.56 and 1.31 mmol/g for As(III)and As(V),respectively.The LDH was effective over a wide pH range for As(III)adsorption(pH 3-8.5)and As(V)adsorption(pH 3-6.5).Using a combination of spectroscopy and sorption modeling calculations,the main sorption mechanism of As(III)and As(V)on SA/MgFe-LDH was identified as inner-sphere complexation via ligand exchangewith hydroxyl group(-OH)and RCOO−.Specifically,bidentate As-Fe complexeswere proposed for both As(III)and As(V)uptake,with the magnitude of formation varying with the initial As concentration.Importantly,the As-laden adsorbent had satisfactory stability in simulated real landfill leachate.These findings demonstrate that SA/MgFe-LDH exhibits considerable potential for remediation of As-contaminated water.展开更多
A nanomicelle(denoted as TPGS/Ppa)was fabricated via the coassembly of the amphiphilic D-α-tocopheryl polyethylene glycol 1000 succinate(TPGS)and the hydrophobic photosensitizer pyropheophorbide a(Ppa)for photodynami...A nanomicelle(denoted as TPGS/Ppa)was fabricated via the coassembly of the amphiphilic D-α-tocopheryl polyethylene glycol 1000 succinate(TPGS)and the hydrophobic photosensitizer pyropheophorbide a(Ppa)for photodynamic therapy(PDT).The obtained nanomicelle possessed a spherical structure with a diameter of(18.0±2.2)nm and a zeta potential of approximately -18 mV.Besides,the nanomicelle exhibited excellent photostability,biocompatibility,and phototoxicity,and could effectively reach the tumor region via the enhanced permeability and retention effect.Additionally,it could be found that the TPGS/Ppa nanomicelle exhibited higher phototoxicity against 4T1 murine mammary cancer cells than free Ppa.In the 4T1 tumor-bearing mouse model,the nanomicelle showed an excellent antitumor therapeutic effect.This study develops a new type of photodynamic nanomicelle TPGS/Ppa,which can increase the accumulation of drugs and prolong their tumor retention time,providing a feasible strategy for realizing the delivery of small-molecule hydrophobic drugs and tumor PDT.展开更多
Aging involves the accumulation of various forms of molecular and cellular damage over time.Key features of aging,such as mitochondrial dysfunction,dysbiosis,and oxidative stress,are closely linked and largely driven ...Aging involves the accumulation of various forms of molecular and cellular damage over time.Key features of aging,such as mitochondrial dysfunction,dysbiosis,and oxidative stress,are closely linked and largely driven by inflammation.This study examines the role of succinate,a key metabolite produced and utilized by cells of both host and microbes,and its receptor,succinate receptor 1(SUCNR1),in age-related oral dysbiosis and inflammation.We examined young and aged wild-type(WT)and SUCNR1 knockout(KO)mice for this analysis.Our findings revealed significant aging-associated alveolar bone loss and succinate elevation in aged WT mice,along with notable changes in the oral microbiome.Conversely,aged KO mice showed reduced bone loss,lower succinate levels,less inflammation,and better-maintained microbial function.These results suggest that SUCNR1 is crucial in influencing aging-related succinate elevation,oral dysbiosis,and inflammation.Analysis of gene families and pathways in the oral microbiome demonstrated distinct aging-related changes between WT and KO mice,with the functional potential being preserved in the KO-aged group.This study underscores the importance of succinate elevation and signaling through SUCNR1 in regulating inflammation,alveolar bone loss,and shifts in the oral microbiome,offering potential targets for therapeutic interventions in age-related oral health issues.展开更多
The materialization of polybutylene succinate(PBS)belongs to the family of polyesters which are degrad-able and biodegradable,their biodegradability properties have attracted enormous interest for product development ...The materialization of polybutylene succinate(PBS)belongs to the family of polyesters which are degrad-able and biodegradable,their biodegradability properties have attracted enormous interest for product development towards different polymer-based applications.Besides its biodegradability,PBS can be derived from petroleum and biobased monomers.At the same time,the latter is the driving factor for its growing interest in bioplastics for fully green and sustainable biobased-derived polymer products.The processes and techniques presented herein,are based on the production of biobased succinic acid monomer to PBS.However,the counterpart biobased monomer 1,4-butanediol(1,4-BDO)production has not been commercially demonstrated.This review discusses the progress in state-of-the-art developments in the synthesis strategies of PBS,its copolymers,and composites with the view to improve molecular weight,thermal,and mechanical properties.It further analyzes the different strategies to synthesize modified PBS polymer composites from organic and inorganic nanofillers to enhance their chemical,thermal,stability and mechanical structural properties.Importantly,the review highlights the progress in the applications of PBS copolymers and composites with tailored structure-designed properties for specific sectors such as packaging films,biomedical and drug release,fire retardants,and agricultural products.The structure-functional performance characteristics of these developments in the PBS,copolymers,and composites are highlighted to provide baseline insights for future developments in engineering the specific applications,and structural interface PBS composites with enhanced structure-functional performance properties.展开更多
[Objectives]To observe the clinical efficacy of external application of Scorzonera Herpes Ointment combined with Methylprednisolone Sodium Succinate for Injection in the treatment of herpes zoster oticus(HZO).[Methods...[Objectives]To observe the clinical efficacy of external application of Scorzonera Herpes Ointment combined with Methylprednisolone Sodium Succinate for Injection in the treatment of herpes zoster oticus(HZO).[Methods]A total of 100 HZO patients admitted to the 988 th Hospital of the Joint Logistics Support Force and Henan Provincial People's Hospital from June 2021 to June 2023 were selected.They were divided into a treatment group and a control group using a random number table method,with 50 cases in each group.Both groups received Methylprednisolone Sodium Succinate for Injection.Additionally,the treatment group was treated with external application of Scorzonera Herpes Ointment,while the control group received acyclovir ointment.Both groups were treated for 10 d.The comparisons included clinical efficacy,total symptom and sign scores,pain level[Visual Analogue Scale(VAS)],time for erythema reduction,cessation of blister formation,scab formation,and scab shedding,incidence of post-herpetic neuralgia(PHN),air conduction hearing threshold,and air-bone gap.[Results]After 10 d of treatment,the total effective rate was 98.00%(49/50)in the treatment group and 84.00%(42/50)in the control group,with a statistically significant difference between the two groups(P<0.05).After 10 d of treatment,the total symptom and sign scores and VAS scores of both groups decreased compared to those before treatment.The treatment group had significantly lower scores than the control group(P<0.05).The treatment group showed significantly shorter time for erythema reduction,cessation of blister formation,scab formation,and scab shedding compared to the control group(P<0.05).During the 1-month follow-up after treatment,no PHN cases occurred in the treatment group,while the incidence of PHN in the control group was 24.00%(12/50),showing a statistically significant difference(P<0.05).After 10 d of treatment,both groups showed reduced air conduction hearing thresholds,and the treatment group exhibited significantly lower air conduction thresholds and air-bone gaps compared to the control group(P<0.05).No statistically significant difference was observed in the air-bone gap before and after treatment in the control group(P>0.05).[Conclusions]The combination of external application of Scorzonera Herpes Ointment and Methylprednisolone Sodium Succinate for Injection can alleviate pain and other discomforts,reduce PHN incidence,shorten disease duration,and improve hearing in HZO patients.展开更多
Liposomal drugs have significantly improved cancer treatment in recent years.However,the clinical application of conventional liposomes is limited by factors such as the complexity of the preparation process and the m...Liposomal drugs have significantly improved cancer treatment in recent years.However,the clinical application of conventional liposomes is limited by factors such as the complexity of the preparation process and the multitude of auxiliary components.By replacing phospholipids and cholesterol with vitamin E succinate(VES),this study addresses these shortcomings by developing a novel modified nanoprodrug,and the new formulation is used to deliver cisplatin.Concurrently,liposomes encapsulating cisplatin were prepared by conventional formulations for comparative experiments.Moreover,VES can inhibit the expression of mitochondrial uncoupling protein 2(UCP2),further enhancing mitochondrial damage in tumor cells within the tumor microenvironment(TME)and suppressing the tricarboxylic acid cycle,thereby reducing ATP production.Additionally,cisplatin damages DNA structure,affecting the binding of Nrf2 to the antioxidant response element(ARE),thereby inhibiting the signaling expression of heme oxygenase1(HO-1).The combined action of cisplatin and VES disrupts the redox balanceleading to a significant accumulation of reactive oxygen species(ROS).The nanoprodrug effectively alters the redox state of the TME and inhibits antioxidant defenses,thereby amplifying oxidative stress damage and enhancing the efficacy of cisplatin.Notably,compared to free cisplatin,the nanoprodrug demonstrates greater efficacy in both cell line-derived xenograft(CDX)and patient-derived tumor xenograft(PDX)liver cancer models.Overall,this study successfully develops a novel mitochondrial-targeted nanoprodrug by modifying the conventional liposome formulation.This provides a new strategy for amplifying oxidative stress in order to disrupt redox balance,and enhance cisplatin efficacy.展开更多
High mortality of choroidal melanoma(CM)is mainly attributed to the high likelihood of tumorous recurrence.The essential challenge lies in the presence of residual CM cells survived from the antitumor treatment.These ...High mortality of choroidal melanoma(CM)is mainly attributed to the high likelihood of tumorous recurrence.The essential challenge lies in the presence of residual CM cells survived from the antitumor treatment.These residual tumorous cells are most likely to cause tumorous recurrence.This article reports the preparation of a multifunctional nanocomposite which can be used to treat CM efficiently via a chemotherapyassisted-photothermal therapy(CTH-PTT).The nanocomposite comprises of alpha-tocopheryl succinate(α-TOS)and carboxylic chitosan modified graphene(CG).α-TOS has been potentially seen as an efficient CTH antitumor drug while its deficiency such as easy being hydrolyzed by gastrointestinal esterase and poor hydrophilicity inevitable limits the clinic application ofα-TOS.CG is introduced to overcome these shortcomings,offering additional advantages such as the PTT possibility for the antitumor application.The employment of CG-α-TOS on ocular CM cells caused more than 80%inhibition rates after irradiation under an 808 nm laser for 10 min.The outcomes of this work provide a facile and advantageous way to resolve the essential issue of the treatment of ocular tumors such as CM.展开更多
Rice bakanae disease(RBD)is a devastating plant disease caused by Fusarium fujikuroi.This study aimed to evaluate the potential of cyclobutrifuram,a novel succinate dehydrogenase inhibitor(SDHI),to control RBD,and det...Rice bakanae disease(RBD)is a devastating plant disease caused by Fusarium fujikuroi.This study aimed to evaluate the potential of cyclobutrifuram,a novel succinate dehydrogenase inhibitor(SDHI),to control RBD,and determine the risk and mechanism of resistance to cyclobutrifuram in F.fujikuroi.In vitro experiments showed that cyclobutrifuram significantly inhibited mycelial growth and spore germination,and altered the morphology of mycelia and conidia.Treatment with cyclobutrifuram signifcantly decreased mycotoxin production and increased cell membrane permeability in F.fujikuroi.The baseline sensitivity of 72 F.fujikuroi isolates to cyclobutrifuram was determined using mycelial growth and spore germination inhibition assays,which revealed EC50values of 0.0114-0.1304 and 0.0012-0.016μg mL^(-1),with mean EC50values of(0.0410±0.0470)and(0.0038±0.0015)μg mL^(-1),respectively.Pot experiments demonstrated that the protective effect of cyclobutrifluram against F.fujikuroi was more significant than that of phenamacril and azoxystrobin,indicating that cyclobutrifuram is a promising antifungal agent for the control of RBD.Six cyclobutrifuramresistant mutants of F.fujikuroi were obtained via fungicide adaptation.Moreover,these mutants exhibited weaker ftness than their parental isolate and positive cross-resistance with other SDHI fungicides,including pydifumetofen and penfufen;however,no cross-resistance was detected with other classes of fungicides,including phenamacril,fudioxonil,prochloraz,or azoxystrobin.These results indicated that the resistance risk of F.fujikuroi to cyclobutrifuram might be moderate.Sequencing analysis revealed that mutations,including H248D in Ff Sdh B,A83V in Ff Sdh C2,and S106F and E166K in Ff Sdh D,contributed to resistance,which was confrmed by molecular docking and homologous replacement experiments.The results suggest a high potential for cyclobutrifuram to control RBD and a moderate resistance risk of F.fujikuroi to cyclobutrifuram,which are meaningful fndings for the scientifc application of cyclobutrifuram.展开更多
As the“fourth messenger”of epigenetic regulation,metabolites playa spatiotemporally specific regulatory role in kidney regeneration by dynamically reshaping the state of chromatin modifications.This review systemati...As the“fourth messenger”of epigenetic regulation,metabolites playa spatiotemporally specific regulatory role in kidney regeneration by dynamically reshaping the state of chromatin modifications.This review systematically expounds the coordinated mechanism of the dual axes of succinate/H3K9ac andα-ketoglutarate(α-KG)/TET enzymes:Succinate activates regeneration-related genes by regulating histone acetylation(H3K9ac),whileα-KG relieves the epigenetic repression of the Wnt pathway through TET-mediated DNA demethylation.The dynamic balance between the two maintains epigenetic plasticity.Multi-omics integration strategies(such as Gaussian graphical models and deep learning frameworks)and single-cell epigenetic tracking technologies(such as spatial metabolomics)have revealed the regulation of metabolite gradients on cellular heterogeneity and the immune microenvironment.The coordinated application of metabolite precursor supplementation(such as NAD precursors)and dynamic monitoring systems(such as isotope tracing and artifi cial intelligence models)has promoted the shift of metabolic medicine from the“static replacement”paradigm to the“dynamic reshaping”paradigm.However,technical bottlenecks(such as insuffi cient multimodal integration)and clinical translation pitfalls(such as challenges in standardized production)still need to be overcome.In the future,through the development of“metabolism-immunity”co-regulatory strategies and intelligent closed-loop systems,it is expected to achieve precise interventions for kidney regeneration and disease treatment.展开更多
In order to recover propylene carbonate(PC)from waste lithium-ion battery electrolyte,the electrolyte was coupled with dimethyl succinate(DMSu)to prepare degradable polyester PPS.To further enhance the performance of ...In order to recover propylene carbonate(PC)from waste lithium-ion battery electrolyte,the electrolyte was coupled with dimethyl succinate(DMSu)to prepare degradable polyester PPS.To further enhance the performance of PPS,ethylene glycol(EG)was introduced to prepare copolyester PPSG,and the effects of the amount of added EG on the performance of copolyester were explored.The results show that the synthesized polyester was the target product.With the increase in the amount of added EG,the molecular weight of the copolyester gradually rose,but the glass transition temperature(T g)of PPSG gradually decreased,and the degradation rate was significantly faster than that of PPS.Within 24 d,the mass loss rate of PPSG-30%reached 75.8%.展开更多
Ni/Al2O3 catalysts were derived from spinel NiAl2O4 with different Ni content ((2.5, 5 and 7.5) wt%). The catalysts were obtained by H-2 reduction and were investigated for the low-temperature hydrogenation of maleic ...Ni/Al2O3 catalysts were derived from spinel NiAl2O4 with different Ni content ((2.5, 5 and 7.5) wt%). The catalysts were obtained by H-2 reduction and were investigated for the low-temperature hydrogenation of maleic anhydride (MA) to produce succinic anhydride (SA). The characterization results showed that Ni-0 active sites were mainly derived during the H2 reduction from spinel NiAl2O4 Among the catalysts studied, employing the optimum preparation and reaction conditions with Ni(5%)/Al2O3 yielded the highest catalytic performance. A near-100% conversion of MA and similar to 90% selectivity to SA were achieved at 120 degrees C and 0.5 MPa of H-2 with a weighted hourly space velocity (MA) of 2 h(-1). (C) 2017, Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.展开更多
We describe a specific procedure for the synthesis of deuterium-labelled 2-（1-methylalkyl）succinate established via alkylation of diethyl malonate, Krapcho decarboxylation reaction with D20 and hydrolysis reaction. ...We describe a specific procedure for the synthesis of deuterium-labelled 2-（1-methylalkyl）succinate established via alkylation of diethyl malonate, Krapcho decarboxylation reaction with D20 and hydrolysis reaction. Two novel compounds, 2-[^2H]-2-ethylsuccinic acid and 2-[^2H]-2-（1-methylhep- tyl）succinic acid were prepared via this synthetic route and characterized by mass spectrometry and ^1H NMR. The results showed that the 2-（1-methylalkyl）succinic acids were deuterated at the β-position, which is considered as an important reaction centre in the anaerobic degradation of n-alkanes.展开更多
The miscibility and crystallization of solution casting biodegradable poly（3-hydroxybuty- rate）/poly（ethylene succinate） （PHB/PES） blends was investigated by differential scanning calorimetry, rheology, and opti...The miscibility and crystallization of solution casting biodegradable poly（3-hydroxybuty- rate）/poly（ethylene succinate） （PHB/PES） blends was investigated by differential scanning calorimetry, rheology, and optical microscopy. The blends showed two glass transition temperatures and a depression of melting temperature of PHB with compositions in phase diagram, which indicated that the blend was partially miscible. The morphology observation supported this result. It was found that the PHB and PES can crystallize simultaneously or upon stepwise depending on the crystallization temperatures and compositions. The spherulite growth rate of PHB increased with increasing of PES content. The influence of compositions on the spherulitic growth rate for the partially miscible polymer blends was discussed.展开更多
Vitamin E succinate was synthesized in organic solvents using a modified Novozym-435 as catalyst.In order to improve the catalytic performance of Novozym-435,the enzyme was modified using acetic anhydride, propionic a...Vitamin E succinate was synthesized in organic solvents using a modified Novozym-435 as catalyst.In order to improve the catalytic performance of Novozym-435,the enzyme was modified using acetic anhydride, propionic anhydride and succinic anhydride separately.We found that both the hydrolytic activity and the thermal stability of the modified Novozym-435 were enhanced compared with the unmodified enzyme.The modified Novozym-435 catalysts were used to synthesize the succinate derivative of vitamin E.Compared with the native Novozym-435,the catalytic activity of the modified novozym-435 in promoting the synthesis of vitamin E succinate was dramatically increased,with the novozym-435 modified with succinic anhydride（N435-S）as the most active catalyst.Conditions for the synthesis of vitamin E succinate were also optimized.A mixture of tert-butanol and DMSO（volume ratio of 2︰3）was the most suitable medium for the reaction,whereas the appropriate molar ratio of vitamin E to succinic anhydride and reaction temperature were 1︰5 and 40°C,respectively.Under these reaction conditions,the yield of vitamin E succinate reached 94.4%.N435-S could be reused for five batches.展开更多
文摘In this study,we investigated improving the performance of a layered double hydroxide(LDH)for the adsorption of As(III)and As(V)by controlling the morphology of LDH crystals.The LDH was synthesized via a simple coprecipitation method using barely soluble MgO as a precursor and succinic acid(SA)as a morphological control agent.Doping the LDH crystals with carboxylate ions(RCOO−)derived from SA caused the crystals to develop in a radial direction.This changed the pore characteristics and increased the density of active surface sites.Subsequently,SA/MgFe-LDH showed excellent affinity for As(III)and As(V)with maximum sorption densities of 2.42 and 1.60 mmol/g,respectively.By comparison,the pristine MgFe-LDH had sorption capacities of 1.56 and 1.31 mmol/g for As(III)and As(V),respectively.The LDH was effective over a wide pH range for As(III)adsorption(pH 3-8.5)and As(V)adsorption(pH 3-6.5).Using a combination of spectroscopy and sorption modeling calculations,the main sorption mechanism of As(III)and As(V)on SA/MgFe-LDH was identified as inner-sphere complexation via ligand exchangewith hydroxyl group(-OH)and RCOO−.Specifically,bidentate As-Fe complexeswere proposed for both As(III)and As(V)uptake,with the magnitude of formation varying with the initial As concentration.Importantly,the As-laden adsorbent had satisfactory stability in simulated real landfill leachate.These findings demonstrate that SA/MgFe-LDH exhibits considerable potential for remediation of As-contaminated water.
文摘A nanomicelle(denoted as TPGS/Ppa)was fabricated via the coassembly of the amphiphilic D-α-tocopheryl polyethylene glycol 1000 succinate(TPGS)and the hydrophobic photosensitizer pyropheophorbide a(Ppa)for photodynamic therapy(PDT).The obtained nanomicelle possessed a spherical structure with a diameter of(18.0±2.2)nm and a zeta potential of approximately -18 mV.Besides,the nanomicelle exhibited excellent photostability,biocompatibility,and phototoxicity,and could effectively reach the tumor region via the enhanced permeability and retention effect.Additionally,it could be found that the TPGS/Ppa nanomicelle exhibited higher phototoxicity against 4T1 murine mammary cancer cells than free Ppa.In the 4T1 tumor-bearing mouse model,the nanomicelle showed an excellent antitumor therapeutic effect.This study develops a new type of photodynamic nanomicelle TPGS/Ppa,which can increase the accumulation of drugs and prolong their tumor retention time,providing a feasible strategy for realizing the delivery of small-molecule hydrophobic drugs and tumor PDT.
基金supported by NIH grants DE027074,DE028212,and AG055787 to Xin Li
文摘Aging involves the accumulation of various forms of molecular and cellular damage over time.Key features of aging,such as mitochondrial dysfunction,dysbiosis,and oxidative stress,are closely linked and largely driven by inflammation.This study examines the role of succinate,a key metabolite produced and utilized by cells of both host and microbes,and its receptor,succinate receptor 1(SUCNR1),in age-related oral dysbiosis and inflammation.We examined young and aged wild-type(WT)and SUCNR1 knockout(KO)mice for this analysis.Our findings revealed significant aging-associated alveolar bone loss and succinate elevation in aged WT mice,along with notable changes in the oral microbiome.Conversely,aged KO mice showed reduced bone loss,lower succinate levels,less inflammation,and better-maintained microbial function.These results suggest that SUCNR1 is crucial in influencing aging-related succinate elevation,oral dysbiosis,and inflammation.Analysis of gene families and pathways in the oral microbiome demonstrated distinct aging-related changes between WT and KO mice,with the functional potential being preserved in the KO-aged group.This study underscores the importance of succinate elevation and signaling through SUCNR1 in regulating inflammation,alveolar bone loss,and shifts in the oral microbiome,offering potential targets for therapeutic interventions in age-related oral health issues.
文摘The materialization of polybutylene succinate(PBS)belongs to the family of polyesters which are degrad-able and biodegradable,their biodegradability properties have attracted enormous interest for product development towards different polymer-based applications.Besides its biodegradability,PBS can be derived from petroleum and biobased monomers.At the same time,the latter is the driving factor for its growing interest in bioplastics for fully green and sustainable biobased-derived polymer products.The processes and techniques presented herein,are based on the production of biobased succinic acid monomer to PBS.However,the counterpart biobased monomer 1,4-butanediol(1,4-BDO)production has not been commercially demonstrated.This review discusses the progress in state-of-the-art developments in the synthesis strategies of PBS,its copolymers,and composites with the view to improve molecular weight,thermal,and mechanical properties.It further analyzes the different strategies to synthesize modified PBS polymer composites from organic and inorganic nanofillers to enhance their chemical,thermal,stability and mechanical structural properties.Importantly,the review highlights the progress in the applications of PBS copolymers and composites with tailored structure-designed properties for specific sectors such as packaging films,biomedical and drug release,fire retardants,and agricultural products.The structure-functional performance characteristics of these developments in the PBS,copolymers,and composites are highlighted to provide baseline insights for future developments in engineering the specific applications,and structural interface PBS composites with enhanced structure-functional performance properties.
基金Supported by the Joint Construction Project of Medical Science and Technology Research Plan in Henan Province(LHGJ20190879).
文摘[Objectives]To observe the clinical efficacy of external application of Scorzonera Herpes Ointment combined with Methylprednisolone Sodium Succinate for Injection in the treatment of herpes zoster oticus(HZO).[Methods]A total of 100 HZO patients admitted to the 988 th Hospital of the Joint Logistics Support Force and Henan Provincial People's Hospital from June 2021 to June 2023 were selected.They were divided into a treatment group and a control group using a random number table method,with 50 cases in each group.Both groups received Methylprednisolone Sodium Succinate for Injection.Additionally,the treatment group was treated with external application of Scorzonera Herpes Ointment,while the control group received acyclovir ointment.Both groups were treated for 10 d.The comparisons included clinical efficacy,total symptom and sign scores,pain level[Visual Analogue Scale(VAS)],time for erythema reduction,cessation of blister formation,scab formation,and scab shedding,incidence of post-herpetic neuralgia(PHN),air conduction hearing threshold,and air-bone gap.[Results]After 10 d of treatment,the total effective rate was 98.00%(49/50)in the treatment group and 84.00%(42/50)in the control group,with a statistically significant difference between the two groups(P<0.05).After 10 d of treatment,the total symptom and sign scores and VAS scores of both groups decreased compared to those before treatment.The treatment group had significantly lower scores than the control group(P<0.05).The treatment group showed significantly shorter time for erythema reduction,cessation of blister formation,scab formation,and scab shedding compared to the control group(P<0.05).During the 1-month follow-up after treatment,no PHN cases occurred in the treatment group,while the incidence of PHN in the control group was 24.00%(12/50),showing a statistically significant difference(P<0.05).After 10 d of treatment,both groups showed reduced air conduction hearing thresholds,and the treatment group exhibited significantly lower air conduction thresholds and air-bone gaps compared to the control group(P<0.05).No statistically significant difference was observed in the air-bone gap before and after treatment in the control group(P>0.05).[Conclusions]The combination of external application of Scorzonera Herpes Ointment and Methylprednisolone Sodium Succinate for Injection can alleviate pain and other discomforts,reduce PHN incidence,shorten disease duration,and improve hearing in HZO patients.
基金financed by National Natural Science Foundation of China(Nos.81773642,52073139)Guangdong Provincial Clinical Research Center for Laboratory Medicine(No.2023B110008)the Provincial subsidies for the construction of high-level hospitals(No.K202201)。
文摘Liposomal drugs have significantly improved cancer treatment in recent years.However,the clinical application of conventional liposomes is limited by factors such as the complexity of the preparation process and the multitude of auxiliary components.By replacing phospholipids and cholesterol with vitamin E succinate(VES),this study addresses these shortcomings by developing a novel modified nanoprodrug,and the new formulation is used to deliver cisplatin.Concurrently,liposomes encapsulating cisplatin were prepared by conventional formulations for comparative experiments.Moreover,VES can inhibit the expression of mitochondrial uncoupling protein 2(UCP2),further enhancing mitochondrial damage in tumor cells within the tumor microenvironment(TME)and suppressing the tricarboxylic acid cycle,thereby reducing ATP production.Additionally,cisplatin damages DNA structure,affecting the binding of Nrf2 to the antioxidant response element(ARE),thereby inhibiting the signaling expression of heme oxygenase1(HO-1).The combined action of cisplatin and VES disrupts the redox balanceleading to a significant accumulation of reactive oxygen species(ROS).The nanoprodrug effectively alters the redox state of the TME and inhibits antioxidant defenses,thereby amplifying oxidative stress damage and enhancing the efficacy of cisplatin.Notably,compared to free cisplatin,the nanoprodrug demonstrates greater efficacy in both cell line-derived xenograft(CDX)and patient-derived tumor xenograft(PDX)liver cancer models.Overall,this study successfully develops a novel mitochondrial-targeted nanoprodrug by modifying the conventional liposome formulation.This provides a new strategy for amplifying oxidative stress in order to disrupt redox balance,and enhance cisplatin efficacy.
基金financially supported by the National Natural Science Foundation of China(Nos.82202354,U20A20338)the Summit Advancement Disciplines of Zhejiang Province(Wenzhou Medical University Pharmaceutics)Key R&D Program of Zhejiang Province(No.2021C04019)。
文摘High mortality of choroidal melanoma(CM)is mainly attributed to the high likelihood of tumorous recurrence.The essential challenge lies in the presence of residual CM cells survived from the antitumor treatment.These residual tumorous cells are most likely to cause tumorous recurrence.This article reports the preparation of a multifunctional nanocomposite which can be used to treat CM efficiently via a chemotherapyassisted-photothermal therapy(CTH-PTT).The nanocomposite comprises of alpha-tocopheryl succinate(α-TOS)and carboxylic chitosan modified graphene(CG).α-TOS has been potentially seen as an efficient CTH antitumor drug while its deficiency such as easy being hydrolyzed by gastrointestinal esterase and poor hydrophilicity inevitable limits the clinic application ofα-TOS.CG is introduced to overcome these shortcomings,offering additional advantages such as the PTT possibility for the antitumor application.The employment of CG-α-TOS on ocular CM cells caused more than 80%inhibition rates after irradiation under an 808 nm laser for 10 min.The outcomes of this work provide a facile and advantageous way to resolve the essential issue of the treatment of ocular tumors such as CM.
基金the National Natural Science Fundation of China(32302392)the Key Project of the Natural Science Foundation of Anhui Provincial Department of Education,China(2022AH050877 and 2023AH040129)+4 种基金the Programs for the Scientific Research Activities of Academic and Technical Leaders of Anhui Province,China(2020D251)the Development Fund for Talent Personnel of Anhui Agricultural University,China(rc342006 and rc342008)the University Synergy Innovation Program of Anhui Province,China(GXXT-2021-059)the Grants from Anhui Agricultural University,China(2020zd27)the Anhui Province Agricultural Eco-Environmental Protection and Quality Safety Industry Technology System,China。
文摘Rice bakanae disease(RBD)is a devastating plant disease caused by Fusarium fujikuroi.This study aimed to evaluate the potential of cyclobutrifuram,a novel succinate dehydrogenase inhibitor(SDHI),to control RBD,and determine the risk and mechanism of resistance to cyclobutrifuram in F.fujikuroi.In vitro experiments showed that cyclobutrifuram significantly inhibited mycelial growth and spore germination,and altered the morphology of mycelia and conidia.Treatment with cyclobutrifuram signifcantly decreased mycotoxin production and increased cell membrane permeability in F.fujikuroi.The baseline sensitivity of 72 F.fujikuroi isolates to cyclobutrifuram was determined using mycelial growth and spore germination inhibition assays,which revealed EC50values of 0.0114-0.1304 and 0.0012-0.016μg mL^(-1),with mean EC50values of(0.0410±0.0470)and(0.0038±0.0015)μg mL^(-1),respectively.Pot experiments demonstrated that the protective effect of cyclobutrifluram against F.fujikuroi was more significant than that of phenamacril and azoxystrobin,indicating that cyclobutrifuram is a promising antifungal agent for the control of RBD.Six cyclobutrifuramresistant mutants of F.fujikuroi were obtained via fungicide adaptation.Moreover,these mutants exhibited weaker ftness than their parental isolate and positive cross-resistance with other SDHI fungicides,including pydifumetofen and penfufen;however,no cross-resistance was detected with other classes of fungicides,including phenamacril,fudioxonil,prochloraz,or azoxystrobin.These results indicated that the resistance risk of F.fujikuroi to cyclobutrifuram might be moderate.Sequencing analysis revealed that mutations,including H248D in Ff Sdh B,A83V in Ff Sdh C2,and S106F and E166K in Ff Sdh D,contributed to resistance,which was confrmed by molecular docking and homologous replacement experiments.The results suggest a high potential for cyclobutrifuram to control RBD and a moderate resistance risk of F.fujikuroi to cyclobutrifuram,which are meaningful fndings for the scientifc application of cyclobutrifuram.
基金funded by Medical Science Research Project of Hebei(No.20220364).
文摘As the“fourth messenger”of epigenetic regulation,metabolites playa spatiotemporally specific regulatory role in kidney regeneration by dynamically reshaping the state of chromatin modifications.This review systematically expounds the coordinated mechanism of the dual axes of succinate/H3K9ac andα-ketoglutarate(α-KG)/TET enzymes:Succinate activates regeneration-related genes by regulating histone acetylation(H3K9ac),whileα-KG relieves the epigenetic repression of the Wnt pathway through TET-mediated DNA demethylation.The dynamic balance between the two maintains epigenetic plasticity.Multi-omics integration strategies(such as Gaussian graphical models and deep learning frameworks)and single-cell epigenetic tracking technologies(such as spatial metabolomics)have revealed the regulation of metabolite gradients on cellular heterogeneity and the immune microenvironment.The coordinated application of metabolite precursor supplementation(such as NAD precursors)and dynamic monitoring systems(such as isotope tracing and artifi cial intelligence models)has promoted the shift of metabolic medicine from the“static replacement”paradigm to the“dynamic reshaping”paradigm.However,technical bottlenecks(such as insuffi cient multimodal integration)and clinical translation pitfalls(such as challenges in standardized production)still need to be overcome.In the future,through the development of“metabolism-immunity”co-regulatory strategies and intelligent closed-loop systems,it is expected to achieve precise interventions for kidney regeneration and disease treatment.
文摘In order to recover propylene carbonate(PC)from waste lithium-ion battery electrolyte,the electrolyte was coupled with dimethyl succinate(DMSu)to prepare degradable polyester PPS.To further enhance the performance of PPS,ethylene glycol(EG)was introduced to prepare copolyester PPSG,and the effects of the amount of added EG on the performance of copolyester were explored.The results show that the synthesized polyester was the target product.With the increase in the amount of added EG,the molecular weight of the copolyester gradually rose,but the glass transition temperature(T g)of PPSG gradually decreased,and the degradation rate was significantly faster than that of PPS.Within 24 d,the mass loss rate of PPSG-30%reached 75.8%.
基金supported by the National Natural Science Foundation of China(21173050,21371035)SINOPEC(X514005)~~
文摘Ni/Al2O3 catalysts were derived from spinel NiAl2O4 with different Ni content ((2.5, 5 and 7.5) wt%). The catalysts were obtained by H-2 reduction and were investigated for the low-temperature hydrogenation of maleic anhydride (MA) to produce succinic anhydride (SA). The characterization results showed that Ni-0 active sites were mainly derived during the H2 reduction from spinel NiAl2O4 Among the catalysts studied, employing the optimum preparation and reaction conditions with Ni(5%)/Al2O3 yielded the highest catalytic performance. A near-100% conversion of MA and similar to 90% selectivity to SA were achieved at 120 degrees C and 0.5 MPa of H-2 with a weighted hourly space velocity (MA) of 2 h(-1). (C) 2017, Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.
基金supported by the National Natural Science Foundation of China(No.41373070,51174092)the National Natural Science Foundation of China/Research Grants Council Joint Research Fund(No.41161160560)
文摘We describe a specific procedure for the synthesis of deuterium-labelled 2-（1-methylalkyl）succinate established via alkylation of diethyl malonate, Krapcho decarboxylation reaction with D20 and hydrolysis reaction. Two novel compounds, 2-[^2H]-2-ethylsuccinic acid and 2-[^2H]-2-（1-methylhep- tyl）succinic acid were prepared via this synthetic route and characterized by mass spectrometry and ^1H NMR. The results showed that the 2-（1-methylalkyl）succinic acids were deuterated at the β-position, which is considered as an important reaction centre in the anaerobic degradation of n-alkanes.
基金ACKNOWLEDGMENT This work was supported by the Key Science Foundation of Education Ministry of China and the Anhui Science Foundation.
文摘The miscibility and crystallization of solution casting biodegradable poly（3-hydroxybuty- rate）/poly（ethylene succinate） （PHB/PES） blends was investigated by differential scanning calorimetry, rheology, and optical microscopy. The blends showed two glass transition temperatures and a depression of melting temperature of PHB with compositions in phase diagram, which indicated that the blend was partially miscible. The morphology observation supported this result. It was found that the PHB and PES can crystallize simultaneously or upon stepwise depending on the crystallization temperatures and compositions. The spherulite growth rate of PHB increased with increasing of PES content. The influence of compositions on the spherulitic growth rate for the partially miscible polymer blends was discussed.
基金Supported by the Fundamental Research Funds for the Central Universities and the State Key Development Program for Basic Research of China(2007CB714304)
文摘Vitamin E succinate was synthesized in organic solvents using a modified Novozym-435 as catalyst.In order to improve the catalytic performance of Novozym-435,the enzyme was modified using acetic anhydride, propionic anhydride and succinic anhydride separately.We found that both the hydrolytic activity and the thermal stability of the modified Novozym-435 were enhanced compared with the unmodified enzyme.The modified Novozym-435 catalysts were used to synthesize the succinate derivative of vitamin E.Compared with the native Novozym-435,the catalytic activity of the modified novozym-435 in promoting the synthesis of vitamin E succinate was dramatically increased,with the novozym-435 modified with succinic anhydride（N435-S）as the most active catalyst.Conditions for the synthesis of vitamin E succinate were also optimized.A mixture of tert-butanol and DMSO（volume ratio of 2︰3）was the most suitable medium for the reaction,whereas the appropriate molar ratio of vitamin E to succinic anhydride and reaction temperature were 1︰5 and 40°C,respectively.Under these reaction conditions,the yield of vitamin E succinate reached 94.4%.N435-S could be reused for five batches.
