[Objectives]To clone the sucC gene of Vibrio alginolyticus strain HY9901 and conduct the bioinformatics analysis.[Methods]Based on the sucC gene of V.alginolyticus strain HY9901,specific primers were designed to ampli...[Objectives]To clone the sucC gene of Vibrio alginolyticus strain HY9901 and conduct the bioinformatics analysis.[Methods]Based on the sucC gene of V.alginolyticus strain HY9901,specific primers were designed to amplify the full length sequence by PCR and make further analysis.[Results]The theoretical molecular weight of SucC protein was about 41528.45 Da,and the full length was 1167 bp,encoding 388 amino acids.It has no signal peptide and transmembrane region,and has a variety of functional sites.It is predicted that it is mainly located in the cytoplasm,and the ubiquitin and lactate modification sites overlap,and it has high gene homology with Vibrio parahaemolyticus.Theα-helix,random coil and extended strand are the main secondary structures.The similarity between the constructed three-level structure model and the template is high.[Conclusions]This study reveals the structural characteristics and functional potential of SucC protein,and provides a theoretical basis for the study of drug resistance mechanism and prevention strategies.展开更多
Tsering Dorje:I Dream of Climbing Mt. Qomolangma Carrying the Olympic Torch Tsering Dorje,the oldest of the Tibetan professional mountaineers,has successfully reached a total of fourteen of the world's highest mou...Tsering Dorje:I Dream of Climbing Mt. Qomolangma Carrying the Olympic Torch Tsering Dorje,the oldest of the Tibetan professional mountaineers,has successfully reached a total of fourteen of the world's highest mountain summits.His companions jokingly refer to him as"Aku"(meaning"uncle"in Tibetan).However, acting as an uncle,he has to shoulder the responsibilities of team leader to take care of the others.展开更多
China’s women tennis players have their eyes set on the Olympics after a year of history making achievements Top Asian women’s tennis doubles partnership Li Na and Zheng Jie,
基金Supported by National Natural Science Foundation of China(32073015)Graduate Education Innovation Program of Guangdong Province(YJYH[2022]1)+1 种基金Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2024007)Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).
文摘[Objectives]To clone the sucC gene of Vibrio alginolyticus strain HY9901 and conduct the bioinformatics analysis.[Methods]Based on the sucC gene of V.alginolyticus strain HY9901,specific primers were designed to amplify the full length sequence by PCR and make further analysis.[Results]The theoretical molecular weight of SucC protein was about 41528.45 Da,and the full length was 1167 bp,encoding 388 amino acids.It has no signal peptide and transmembrane region,and has a variety of functional sites.It is predicted that it is mainly located in the cytoplasm,and the ubiquitin and lactate modification sites overlap,and it has high gene homology with Vibrio parahaemolyticus.Theα-helix,random coil and extended strand are the main secondary structures.The similarity between the constructed three-level structure model and the template is high.[Conclusions]This study reveals the structural characteristics and functional potential of SucC protein,and provides a theoretical basis for the study of drug resistance mechanism and prevention strategies.
文摘Tsering Dorje:I Dream of Climbing Mt. Qomolangma Carrying the Olympic Torch Tsering Dorje,the oldest of the Tibetan professional mountaineers,has successfully reached a total of fourteen of the world's highest mountain summits.His companions jokingly refer to him as"Aku"(meaning"uncle"in Tibetan).However, acting as an uncle,he has to shoulder the responsibilities of team leader to take care of the others.
文摘China’s women tennis players have their eyes set on the Olympics after a year of history making achievements Top Asian women’s tennis doubles partnership Li Na and Zheng Jie,
