1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated fro...1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated from a 2 year old Bangladeshi child.长双歧杆菌幼儿亚种.展开更多
Potential interactive effects and trade-offs between drivers can regulate species diversity,affecting distributions by several orders of magnitude and distribution.The decomposition ofβ-diversity into turnover and ne...Potential interactive effects and trade-offs between drivers can regulate species diversity,affecting distributions by several orders of magnitude and distribution.The decomposition ofβ-diversity into turnover and nestedness could disentangle community assembly rules and offer the opportunity to encompass the processes that structure the communities and maintain theβ-diversity on the campo rupestre.We evaluatedα-andβ-diversity and species conservation status of rocky outcrop communities in the Parque Nacional da Serra do Cipó,as well as the decomposed component of theβ-diversity index.Due to the isolation characteristics of rocky outcrops,we expect to find high taxonomic turnover among the communities.The study was performed on five quartzitic rocky outcrops divided into two sampling stations.We used field expeditions and plant inventory data of shrubs and herbaceous layers.We recorded 286 angiosperms taxa among rocky outcrops of campo rupestre of Serra do Cipó.The structure andα-diversity showed significant differences among rocky outcrops and an evident oligarchic structure in the plant communities analyzed.Taxonomicβ-diversity showed high turnover with a significant contribution of the turnover component to this index.This pattern reflects the interplay between regional and local scale processes.Therefore,we believe this approach becomes a unified framework,which allows the comparison of diversity patterns and ecological processes in rocky outcrops ecosystems.We highlight the high percentage of not evaluated species for threats and the need to fill this lack.展开更多
Anthropogenic disturbances are causing significant impacts on plant distribution worldwide,and many of these effects are driven by changes in the recruitment patterns of plant species.Global warming and land-use chang...Anthropogenic disturbances are causing significant impacts on plant distribution worldwide,and many of these effects are driven by changes in the recruitment patterns of plant species.Global warming and land-use change are two major disturbances leading to changes in germination strategies by changing both soil temperature regimes and light quality reaching the seeds due to soil disturbance.Investigating the range,overlap,and redundancy of niche germination of co-occurring plant species allows us to understand whether endemic species are threatened either by native non-endemic or by alien species,especially in an ecosystem of restricted distribution such as the campo rupestre.Employing a systematic review,this study aimed to evaluate the effect of temperature increase and seed burial on the germination of endemic and non-endemic species in the campo rupestre in Brazil.We performed a metaanalysis using increased temperature and darkness as proxies for the impact of disturbance on germination patterns.In this context,we hypothesized that:increased temperature and darkness negatively influence the germination of native species and positively influence the germination of alien species in the campo rupestre.Specifically,we expect the negative effect to be more pronounced in endemic species than in native non-endemic species.Moreover,we intend to describe the role of seed size in the germination of native and alien species from campo rupestre in the context of increased temperature and darkness.Our analysis showed that increased temperature influenced the germination of alien species by ca.55%,while it did not influence the germination of endemic or native non-endemic species.Furthermore,the germination of alien species under higher temperatures was promoted by increasing seed size.Darkness negatively influenced seed germination of native species,independent of their distribution.Moreover,under darkness conditions,the germination of endemic seeds decreased with seed size.Through their direct effects on germination strategies,we conclude that warming temperatures and land-use change can lead to a long-term displacement of endemic species by native non-endemic and alien species in campo rupestre,thus compromising ecosystem services and conservation of these fragile physiognomies in the near future.展开更多
Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in...Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in cucumber has not been reported.Here,we investigated the BSR resistance of 119 cucumber core germplasm worldwide at the seedling stage and identified 26 accessions highly resistant to BSR.A total of 1642740 single-nucleotide polymorphisms(SNPs)were used to conduct GWAS,and five loci associated with BSR resistance were detected on four chromosomes:gBSR2.1,gBSR2.2,gBSR3.1,gBSR4.1 and gBSR5.1.Based on haplotype analysis,sequence polymorphisms,functional annotation and qRT-PCR analysis,six candidate genes were identified within the five loci.CsaV3_2G014450,CsaV3_2G014490,CsaV3_2G016000,CsaV3_3G000850,CsaV3_4G033150,and CsaV3_5G000390 each had nonsynonymous SNPs,and were significantly up-regulated in the resistant genotypes after inoculation.And CsaV3_5G000390 in the susceptible genotype was significantly up-regulated after inoculation.The identification of these candidate genes lays a foundation for understanding the genetic mechanism of BSR resistance in cucumber.Generally,our study mined genes associated with BSR resistance in cucumber seedlings and will assist the breeding of BSR-resistant cucumber cultivars.展开更多
Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by g...Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.展开更多
Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effec...Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.展开更多
Microbial food is an important direction of sustainable food development in the future.Microorganisms such as lactic acid bacteria(LAB)are important sources of natural radioprotectors.Moderate environmental stress can...Microbial food is an important direction of sustainable food development in the future.Microorganisms such as lactic acid bacteria(LAB)are important sources of natural radioprotectors.Moderate environmental stress can induce stress response and improve biological activity of LAB.In the previous study,Lactococcus lactis subsp.lactis IL1403(L.lactis IL1403)cell-free extract induced by ionizing radiation(IR)of 500 Gy(IR-CFE)presented the stronger radioprotective effect than untreated cell-free extract in mice.To explore the radioprotective active substances of IR-CFE,the key protein was screened by proteomics and its radioprotective effect in vivo was further evaluated.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment showed that two-component systems(TCS)were enhanced to adapt the IR induction.Meanwhile,the protein LlrG belonged to TCS was screened by the protein-protein interaction.Furthermore,the recombinant protein LlrG(rLlrG)could markedly alleviate the ^(60)Coγ-induced damage to the hematopoietic system,oxidative stress and inflammation in mice,thereby exerting its radioprotective function.These results suggested LlrG protein not only played an important role in the adaptation of L.lactis IL1403 to IR environment,but also exerted a good radioprotective effect,which could be applied in the development of protein-based radioprotectors.展开更多
Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs t...Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs to be further investigated.This study aimed to examine the alleviating effect of Lacticaseibacillus paracasei L9 and Bifidobacterium animalis subsp.lactis A6 and possible mechanism in mice with AD.In this study,the administration of probiotics L9 and A6 effectively improved memory and learning functions in AD mice.The hippocampal cells of mice were partly recovered in morphology and rearranged more neatly after probiotics intervention.Meanwhile,L9 and A6 exhibited inhibitory effects on the phosphorylation of Tau and the deposition of Aβ,which were mediated via GSK-3βand PP2A kinases.Meanwhile,by metagenomic sequencing,we found interventions with L9 and A6 altered the intestinal microbiome's taxonomic composition,reduced the abundance of pathogenic Desulfovibrio genera,and increased beneficial Clostridium and Paramuribaculum genera abundance.The fatty acids metabolism and biosynthesis of gut microbiome were also enhanced.Serum untargeted metabolomics also showed noticeable alternation in lipidrelated metabolites,which may alleviate the pathogenesis of AD.These results revealed a mitigating role for probiotic L9 and A6 in AD prevention and offer new insights into AD prevention via gut-brain connection.展开更多
Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to ...Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.展开更多
【目的】分离鉴定江苏省扬州市养殖场异育银鲫患病病原。【方法】采用常规的理化特性和分子生物学的方法,对从濒死异育银鲫肝脏处分离到的菌株YZ-1进行表型生物学、分子生物学及药敏试验的系统研究。【结果】该菌株16S r RNA基因(序列长...【目的】分离鉴定江苏省扬州市养殖场异育银鲫患病病原。【方法】采用常规的理化特性和分子生物学的方法,对从濒死异育银鲫肝脏处分离到的菌株YZ-1进行表型生物学、分子生物学及药敏试验的系统研究。【结果】该菌株16S r RNA基因(序列长度1 446 bp,Gen Bank登录号为JX164202)与其它杀鲑气单胞菌16S r RNA基因一致性在99%-100%之间,构建发育树确定该菌株为杀鲑气单胞菌杀鲑亚种(Aeromonas salmonicida subsp.salmonicida)。人工回感可导致异育银鲫死亡。药敏试验结果显示:对头孢呋辛、复方新诺明、恩诺沙星等23种抗生素敏感;对阿米卡星、四环素、大观霉素、头孢拉定等11种抗生素中度敏感;对青霉素G、链霉素、庆大霉素、氟苯尼考、万古霉素等10种抗生素耐药。【结论】研究结果证实引起异育银鲫死亡的病原为杀鲑气单胞菌杀鲑亚种。展开更多
文摘1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated from a 2 year old Bangladeshi child.长双歧杆菌幼儿亚种.
文摘以岩青兰化学成分为基础,生信分析筛选岩青兰改善老年糖尿病小鼠脑神经病变的关键靶点及通路,并通过小鼠实验进行验证,明确其作用机制。通过Gene Cards搜集神经炎症靶点,GEO数据库筛选老年糖尿病患者脑相关差异基因(DEGs);用Venny软件得到岩青兰的核心成分靶点与神经炎症交集靶点为56个、与DEGs交集靶点31个;通过STRING数据库构建蛋白相互作用(PPI)网络,利用Cytoscape软件进行PPI及药物-成分-靶点网络的可视化处理,并通过Maximum Clique Centrality(MCC)筛选Top10核心靶点;用DAVID数据库进行GO(gene ontology)功能富集和KEGG(Kyoto encyclopedia of genes and genomes)通路分析,发现岩青兰主要通过神经炎症相关的丝裂原活化蛋白激酶(MAPK)、核转录因子(NF-κB)等通路蛋白改善老年糖尿病神经病变;使用分子对接软件对核心活性成分与核心靶点进行对接发现岩青兰中的绿原酸、香叶木素、山柰酚与蛋白激酶B(AKT1)、白细胞介素-1β(IL-1β)、B淋巴细胞瘤2(BCL2)、血红素加氧酶1(HMOX1)等靶点均有较好的结合活性。动物实验显示,岩青兰可以降低小鼠血糖、血脂水平,改善肝脏组织细胞排列紊乱,脂肪空泡的现象,减少血清促炎细胞因子的水平;下调脑肿瘤坏死因子α(TNF-α)、IL-1β、Toll样受体4(TLR4)、核NF-κB蛋白的表达,抑制小鼠脑离子钙结合衔接分子1(Iba-1)的过度激活,降低NOD样受体热蛋白结构域蛋白3(NLRP3)炎症小体的激活。该研究初步揭示了岩青兰对老年糖尿病脑神经病变的治疗作用可能与调控TLR4/NF-κB、抑制炎症小体等炎症信号通路有关。本实验经过沈阳药科大学伦理委员会批准(No.SYPU-IACOC-2024-1016-103)。
基金the Graduate Program in Ecology, Conservation and Wildlife Management (ECMVS)Institute of Biological Sciences (ICB)+1 种基金Department of Botany, Biodiversitas Foundation, Universidade Federal de Minas GeraisCoordenacao de Aperfeicoamento de Pessoal de Nível Superior (CAPES) for supporting this study
文摘Potential interactive effects and trade-offs between drivers can regulate species diversity,affecting distributions by several orders of magnitude and distribution.The decomposition ofβ-diversity into turnover and nestedness could disentangle community assembly rules and offer the opportunity to encompass the processes that structure the communities and maintain theβ-diversity on the campo rupestre.We evaluatedα-andβ-diversity and species conservation status of rocky outcrop communities in the Parque Nacional da Serra do Cipó,as well as the decomposed component of theβ-diversity index.Due to the isolation characteristics of rocky outcrops,we expect to find high taxonomic turnover among the communities.The study was performed on five quartzitic rocky outcrops divided into two sampling stations.We used field expeditions and plant inventory data of shrubs and herbaceous layers.We recorded 286 angiosperms taxa among rocky outcrops of campo rupestre of Serra do Cipó.The structure andα-diversity showed significant differences among rocky outcrops and an evident oligarchic structure in the plant communities analyzed.Taxonomicβ-diversity showed high turnover with a significant contribution of the turnover component to this index.This pattern reflects the interplay between regional and local scale processes.Therefore,we believe this approach becomes a unified framework,which allows the comparison of diversity patterns and ecological processes in rocky outcrops ecosystems.We highlight the high percentage of not evaluated species for threats and the need to fill this lack.
基金financial support from CNPq/Peld CRSC,Fapemig,CAPES,Anglo American,and Vale.
文摘Anthropogenic disturbances are causing significant impacts on plant distribution worldwide,and many of these effects are driven by changes in the recruitment patterns of plant species.Global warming and land-use change are two major disturbances leading to changes in germination strategies by changing both soil temperature regimes and light quality reaching the seeds due to soil disturbance.Investigating the range,overlap,and redundancy of niche germination of co-occurring plant species allows us to understand whether endemic species are threatened either by native non-endemic or by alien species,especially in an ecosystem of restricted distribution such as the campo rupestre.Employing a systematic review,this study aimed to evaluate the effect of temperature increase and seed burial on the germination of endemic and non-endemic species in the campo rupestre in Brazil.We performed a metaanalysis using increased temperature and darkness as proxies for the impact of disturbance on germination patterns.In this context,we hypothesized that:increased temperature and darkness negatively influence the germination of native species and positively influence the germination of alien species in the campo rupestre.Specifically,we expect the negative effect to be more pronounced in endemic species than in native non-endemic species.Moreover,we intend to describe the role of seed size in the germination of native and alien species from campo rupestre in the context of increased temperature and darkness.Our analysis showed that increased temperature influenced the germination of alien species by ca.55%,while it did not influence the germination of endemic or native non-endemic species.Furthermore,the germination of alien species under higher temperatures was promoted by increasing seed size.Darkness negatively influenced seed germination of native species,independent of their distribution.Moreover,under darkness conditions,the germination of endemic seeds decreased with seed size.Through their direct effects on germination strategies,we conclude that warming temperatures and land-use change can lead to a long-term displacement of endemic species by native non-endemic and alien species in campo rupestre,thus compromising ecosystem services and conservation of these fragile physiognomies in the near future.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFD1200101)the Earmarked Fund for Modern Agro-industry Technology Research System(Grant No.CARS-23)Science and Technology Innovation Program of the Chinese Academy of Agricultural Science(Grant No.CAAS-ASTIP-IVFCAAS).
文摘Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in cucumber has not been reported.Here,we investigated the BSR resistance of 119 cucumber core germplasm worldwide at the seedling stage and identified 26 accessions highly resistant to BSR.A total of 1642740 single-nucleotide polymorphisms(SNPs)were used to conduct GWAS,and five loci associated with BSR resistance were detected on four chromosomes:gBSR2.1,gBSR2.2,gBSR3.1,gBSR4.1 and gBSR5.1.Based on haplotype analysis,sequence polymorphisms,functional annotation and qRT-PCR analysis,six candidate genes were identified within the five loci.CsaV3_2G014450,CsaV3_2G014490,CsaV3_2G016000,CsaV3_3G000850,CsaV3_4G033150,and CsaV3_5G000390 each had nonsynonymous SNPs,and were significantly up-regulated in the resistant genotypes after inoculation.And CsaV3_5G000390 in the susceptible genotype was significantly up-regulated after inoculation.The identification of these candidate genes lays a foundation for understanding the genetic mechanism of BSR resistance in cucumber.Generally,our study mined genes associated with BSR resistance in cucumber seedlings and will assist the breeding of BSR-resistant cucumber cultivars.
基金supported by the National Key R&D Programs of China(2022YFF0710701,2022YFF0710702)Guangzhou Key Research and Development Program(202206010157)+2 种基金Guangzhou Joint Fund for Key Laboratory(202201020381)Medical Joint Fund of Jinan University(YXJC202204)Open Research Project of the Key Laboratory of Viral Pathogenesis and Infection Prevention and Control of the Ministry of Education(2023VPPC-R02).
文摘Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.
基金supported by the National Natural Science Foundation of China(32021005,32272332)the Fundamental Research Funds for the Central Universities(JUSRP622020,JUSRP51501)+1 种基金the Program of Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu ProvincePostgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX22_2391)。
文摘Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.
基金supported by the National Natural Science Foundation of China(32172211,32572557)the Key Science Research Projects of Higher Education Institutions in Henan Province(21A530006)+3 种基金the Key Research and Development and Promotion Projects of Henan Province(232102310293)the Henan Provincial Natural Science Foundation General Program(252300421403)the Zhongyuan Sci-Tech Innovation Leading Talents(254200510040)the Innovative Research Team(in Science and Technology)in University of Henan Province(26IRTSTHN030).
文摘Microbial food is an important direction of sustainable food development in the future.Microorganisms such as lactic acid bacteria(LAB)are important sources of natural radioprotectors.Moderate environmental stress can induce stress response and improve biological activity of LAB.In the previous study,Lactococcus lactis subsp.lactis IL1403(L.lactis IL1403)cell-free extract induced by ionizing radiation(IR)of 500 Gy(IR-CFE)presented the stronger radioprotective effect than untreated cell-free extract in mice.To explore the radioprotective active substances of IR-CFE,the key protein was screened by proteomics and its radioprotective effect in vivo was further evaluated.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment showed that two-component systems(TCS)were enhanced to adapt the IR induction.Meanwhile,the protein LlrG belonged to TCS was screened by the protein-protein interaction.Furthermore,the recombinant protein LlrG(rLlrG)could markedly alleviate the ^(60)Coγ-induced damage to the hematopoietic system,oxidative stress and inflammation in mice,thereby exerting its radioprotective function.These results suggested LlrG protein not only played an important role in the adaptation of L.lactis IL1403 to IR environment,but also exerted a good radioprotective effect,which could be applied in the development of protein-based radioprotectors.
基金funded by the National Key R&D Program of China(2023YFF1104501)R&D Program of Beijing Municipal Education Commission(23JF0006)National Natural Science Foundation of China(31601443 and 32101938)。
文摘Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs to be further investigated.This study aimed to examine the alleviating effect of Lacticaseibacillus paracasei L9 and Bifidobacterium animalis subsp.lactis A6 and possible mechanism in mice with AD.In this study,the administration of probiotics L9 and A6 effectively improved memory and learning functions in AD mice.The hippocampal cells of mice were partly recovered in morphology and rearranged more neatly after probiotics intervention.Meanwhile,L9 and A6 exhibited inhibitory effects on the phosphorylation of Tau and the deposition of Aβ,which were mediated via GSK-3βand PP2A kinases.Meanwhile,by metagenomic sequencing,we found interventions with L9 and A6 altered the intestinal microbiome's taxonomic composition,reduced the abundance of pathogenic Desulfovibrio genera,and increased beneficial Clostridium and Paramuribaculum genera abundance.The fatty acids metabolism and biosynthesis of gut microbiome were also enhanced.Serum untargeted metabolomics also showed noticeable alternation in lipidrelated metabolites,which may alleviate the pathogenesis of AD.These results revealed a mitigating role for probiotic L9 and A6 in AD prevention and offer new insights into AD prevention via gut-brain connection.
基金funded by the National Key R&D Program of China(2021YFD2100700)National Natural Science Foundation of China(32021005)+1 种基金111 project(BP0719028)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.
文摘【目的】分离鉴定江苏省扬州市养殖场异育银鲫患病病原。【方法】采用常规的理化特性和分子生物学的方法,对从濒死异育银鲫肝脏处分离到的菌株YZ-1进行表型生物学、分子生物学及药敏试验的系统研究。【结果】该菌株16S r RNA基因(序列长度1 446 bp,Gen Bank登录号为JX164202)与其它杀鲑气单胞菌16S r RNA基因一致性在99%-100%之间,构建发育树确定该菌株为杀鲑气单胞菌杀鲑亚种(Aeromonas salmonicida subsp.salmonicida)。人工回感可导致异育银鲫死亡。药敏试验结果显示:对头孢呋辛、复方新诺明、恩诺沙星等23种抗生素敏感;对阿米卡星、四环素、大观霉素、头孢拉定等11种抗生素中度敏感;对青霉素G、链霉素、庆大霉素、氟苯尼考、万古霉素等10种抗生素耐药。【结论】研究结果证实引起异育银鲫死亡的病原为杀鲑气单胞菌杀鲑亚种。
