The influence of relative humidity (RH) on quasistatic current-voltage (I-V) characteristics of Bifidobacterium animalis subsp. lactis BB-12 thin layers was studied for the first time. The value of electrical conducti...The influence of relative humidity (RH) on quasistatic current-voltage (I-V) characteristics of Bifidobacterium animalis subsp. lactis BB-12 thin layers was studied for the first time. The value of electrical conductivity in 75% RH was found to be in the order of 10-7 (ohm·cm)-1, which was 106 orders of magnitude higher than that observed in dry atmosphere. It was concluded that RH played a key role in hysteresis behavior of the measured (I-V) characteristics. FTIR measurements showed that under water moisture environment, the associated bonds between amine and carboxyl group were greatly strengthened that was the source of free charge carries after ionization. The surface charge of Bifidobacterium animalis subsp. lactis BB-12 was found to be negative by zeta potential measurements, claiming that electrons were the charge carriers.展开更多
Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by g...Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.展开更多
Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs t...Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs to be further investigated.This study aimed to examine the alleviating effect of Lacticaseibacillus paracasei L9 and Bifidobacterium animalis subsp.lactis A6 and possible mechanism in mice with AD.In this study,the administration of probiotics L9 and A6 effectively improved memory and learning functions in AD mice.The hippocampal cells of mice were partly recovered in morphology and rearranged more neatly after probiotics intervention.Meanwhile,L9 and A6 exhibited inhibitory effects on the phosphorylation of Tau and the deposition of Aβ,which were mediated via GSK-3βand PP2A kinases.Meanwhile,by metagenomic sequencing,we found interventions with L9 and A6 altered the intestinal microbiome's taxonomic composition,reduced the abundance of pathogenic Desulfovibrio genera,and increased beneficial Clostridium and Paramuribaculum genera abundance.The fatty acids metabolism and biosynthesis of gut microbiome were also enhanced.Serum untargeted metabolomics also showed noticeable alternation in lipidrelated metabolites,which may alleviate the pathogenesis of AD.These results revealed a mitigating role for probiotic L9 and A6 in AD prevention and offer new insights into AD prevention via gut-brain connection.展开更多
文摘The influence of relative humidity (RH) on quasistatic current-voltage (I-V) characteristics of Bifidobacterium animalis subsp. lactis BB-12 thin layers was studied for the first time. The value of electrical conductivity in 75% RH was found to be in the order of 10-7 (ohm·cm)-1, which was 106 orders of magnitude higher than that observed in dry atmosphere. It was concluded that RH played a key role in hysteresis behavior of the measured (I-V) characteristics. FTIR measurements showed that under water moisture environment, the associated bonds between amine and carboxyl group were greatly strengthened that was the source of free charge carries after ionization. The surface charge of Bifidobacterium animalis subsp. lactis BB-12 was found to be negative by zeta potential measurements, claiming that electrons were the charge carriers.
基金supported by the National Key R&D Programs of China(2022YFF0710701,2022YFF0710702)Guangzhou Key Research and Development Program(202206010157)+2 种基金Guangzhou Joint Fund for Key Laboratory(202201020381)Medical Joint Fund of Jinan University(YXJC202204)Open Research Project of the Key Laboratory of Viral Pathogenesis and Infection Prevention and Control of the Ministry of Education(2023VPPC-R02).
文摘Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.
基金funded by the National Key R&D Program of China(2023YFF1104501)R&D Program of Beijing Municipal Education Commission(23JF0006)National Natural Science Foundation of China(31601443 and 32101938)。
文摘Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs to be further investigated.This study aimed to examine the alleviating effect of Lacticaseibacillus paracasei L9 and Bifidobacterium animalis subsp.lactis A6 and possible mechanism in mice with AD.In this study,the administration of probiotics L9 and A6 effectively improved memory and learning functions in AD mice.The hippocampal cells of mice were partly recovered in morphology and rearranged more neatly after probiotics intervention.Meanwhile,L9 and A6 exhibited inhibitory effects on the phosphorylation of Tau and the deposition of Aβ,which were mediated via GSK-3βand PP2A kinases.Meanwhile,by metagenomic sequencing,we found interventions with L9 and A6 altered the intestinal microbiome's taxonomic composition,reduced the abundance of pathogenic Desulfovibrio genera,and increased beneficial Clostridium and Paramuribaculum genera abundance.The fatty acids metabolism and biosynthesis of gut microbiome were also enhanced.Serum untargeted metabolomics also showed noticeable alternation in lipidrelated metabolites,which may alleviate the pathogenesis of AD.These results revealed a mitigating role for probiotic L9 and A6 in AD prevention and offer new insights into AD prevention via gut-brain connection.
