In May 2016,an epizootic occured among cultured tongue soles caused mass deaths in a fish farm in Qinhuangdao,China.In order to find out the etiological agent,a bacterial strain was isolated from ascites and other tis...In May 2016,an epizootic occured among cultured tongue soles caused mass deaths in a fish farm in Qinhuangdao,China.In order to find out the etiological agent,a bacterial strain was isolated from ascites and other tissues of sick tongue sole aseptically collected.The isolate was identified as Photobacterium damselae subsp.damsela（PDD） by isolation culture,Gram staining,physiological identification,morohological observation,biochemical identification and 16S rDNA sequence analysis.The results showed that the isolate shared 99.6% homology with the reference strain in GenBank.The animal regression test displayed that the isolate had very strong pathogenicity to tongue sole.The LD（50） was 3.1 × 10~4 CFU/mL,and it showed pathogenicity to mammals.The antimicrobial susceptibility test showed the isolate was highly sensitive to nrofloxacin,Norfloxacin,Ciprofloxacin,Mequindox;moderately sensitive to Cefradine,Doxycycline;and insensitive to Gentamicin,Ceftriaxone,Tilmicosin,etc..展开更多
As a marine bacterial pathogen, Photobacterium damselae subsp. damselae(PDD) is distributed in seawater worldwide. It can infect different animals as well as humans, even cause deaths. The highly conserved regions of ...As a marine bacterial pathogen, Photobacterium damselae subsp. damselae(PDD) is distributed in seawater worldwide. It can infect different animals as well as humans, even cause deaths. The highly conserved regions of PDD mcp gene on chromosome and dly gene on plasmid were selected as the target fragments to design the specific primers. Recombinant plasmid standard was prepared based on the primers. With GENECHECKER UF-150 qRT-PCR instrument as the platform, a fluorescence-based quantitative real-time PCR(qRT-PCR) method was established for the detection of PDD. This method can specifically detect PDD and distinguish the highly virulent strains. Additionally, the test results can be qualitatively judged by visualization, while the quantitative detection can be achieved through the standard curve calculation. The minimum limit of detection was 1.0×101 copies μL-1, and the detection time was less than 20 min. In summary, this new method has outstanding advantages, such as strong specificity, high sensitivity, and low site requirements. It is a rapid on-site detection technology for highly virulent PDD strains.展开更多
Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by g...Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.展开更多
1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated fro...1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated from a 2 year old Bangladeshi child.长双歧杆菌幼儿亚种.展开更多
Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in...Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in cucumber has not been reported.Here,we investigated the BSR resistance of 119 cucumber core germplasm worldwide at the seedling stage and identified 26 accessions highly resistant to BSR.A total of 1642740 single-nucleotide polymorphisms(SNPs)were used to conduct GWAS,and five loci associated with BSR resistance were detected on four chromosomes:gBSR2.1,gBSR2.2,gBSR3.1,gBSR4.1 and gBSR5.1.Based on haplotype analysis,sequence polymorphisms,functional annotation and qRT-PCR analysis,six candidate genes were identified within the five loci.CsaV3_2G014450,CsaV3_2G014490,CsaV3_2G016000,CsaV3_3G000850,CsaV3_4G033150,and CsaV3_5G000390 each had nonsynonymous SNPs,and were significantly up-regulated in the resistant genotypes after inoculation.And CsaV3_5G000390 in the susceptible genotype was significantly up-regulated after inoculation.The identification of these candidate genes lays a foundation for understanding the genetic mechanism of BSR resistance in cucumber.Generally,our study mined genes associated with BSR resistance in cucumber seedlings and will assist the breeding of BSR-resistant cucumber cultivars.展开更多
Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effec...Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.展开更多
Microbial food is an important direction of sustainable food development in the future.Microorganisms such as lactic acid bacteria(LAB)are important sources of natural radioprotectors.Moderate environmental stress can...Microbial food is an important direction of sustainable food development in the future.Microorganisms such as lactic acid bacteria(LAB)are important sources of natural radioprotectors.Moderate environmental stress can induce stress response and improve biological activity of LAB.In the previous study,Lactococcus lactis subsp.lactis IL1403(L.lactis IL1403)cell-free extract induced by ionizing radiation(IR)of 500 Gy(IR-CFE)presented the stronger radioprotective effect than untreated cell-free extract in mice.To explore the radioprotective active substances of IR-CFE,the key protein was screened by proteomics and its radioprotective effect in vivo was further evaluated.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment showed that two-component systems(TCS)were enhanced to adapt the IR induction.Meanwhile,the protein LlrG belonged to TCS was screened by the protein-protein interaction.Furthermore,the recombinant protein LlrG(rLlrG)could markedly alleviate the ^(60)Coγ-induced damage to the hematopoietic system,oxidative stress and inflammation in mice,thereby exerting its radioprotective function.These results suggested LlrG protein not only played an important role in the adaptation of L.lactis IL1403 to IR environment,but also exerted a good radioprotective effect,which could be applied in the development of protein-based radioprotectors.展开更多
Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs t...Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs to be further investigated.This study aimed to examine the alleviating effect of Lacticaseibacillus paracasei L9 and Bifidobacterium animalis subsp.lactis A6 and possible mechanism in mice with AD.In this study,the administration of probiotics L9 and A6 effectively improved memory and learning functions in AD mice.The hippocampal cells of mice were partly recovered in morphology and rearranged more neatly after probiotics intervention.Meanwhile,L9 and A6 exhibited inhibitory effects on the phosphorylation of Tau and the deposition of Aβ,which were mediated via GSK-3βand PP2A kinases.Meanwhile,by metagenomic sequencing,we found interventions with L9 and A6 altered the intestinal microbiome's taxonomic composition,reduced the abundance of pathogenic Desulfovibrio genera,and increased beneficial Clostridium and Paramuribaculum genera abundance.The fatty acids metabolism and biosynthesis of gut microbiome were also enhanced.Serum untargeted metabolomics also showed noticeable alternation in lipidrelated metabolites,which may alleviate the pathogenesis of AD.These results revealed a mitigating role for probiotic L9 and A6 in AD prevention and offer new insights into AD prevention via gut-brain connection.展开更多
Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to ...Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.展开更多
基金Supported by Incentive Subsidy Program of Hebei Department of Science and Technology(15926620H)Key Technology R&D Program of Qinhuangdao Science and Technology Bureau(201401A067)+1 种基金Prevention and Control of Major Bacterial Diseases in Industrial Farming Fishes(201602A341)Sereening and Preliminary Application of Protective Antigen of Two Important Marine Pathogens(2018HY007)
文摘In May 2016,an epizootic occured among cultured tongue soles caused mass deaths in a fish farm in Qinhuangdao,China.In order to find out the etiological agent,a bacterial strain was isolated from ascites and other tissues of sick tongue sole aseptically collected.The isolate was identified as Photobacterium damselae subsp.damsela（PDD） by isolation culture,Gram staining,physiological identification,morohological observation,biochemical identification and 16S rDNA sequence analysis.The results showed that the isolate shared 99.6% homology with the reference strain in GenBank.The animal regression test displayed that the isolate had very strong pathogenicity to tongue sole.The LD（50） was 3.1 × 10~4 CFU/mL,and it showed pathogenicity to mammals.The antimicrobial susceptibility test showed the isolate was highly sensitive to nrofloxacin,Norfloxacin,Ciprofloxacin,Mequindox;moderately sensitive to Cefradine,Doxycycline;and insensitive to Gentamicin,Ceftriaxone,Tilmicosin,etc..
基金supported by the National Key Research and Development Program of China (No. 2019YFD0900104)the Key Projects of Science and Technology In-novation of Shandong Province (No. 2018YFJH0703)。
文摘As a marine bacterial pathogen, Photobacterium damselae subsp. damselae(PDD) is distributed in seawater worldwide. It can infect different animals as well as humans, even cause deaths. The highly conserved regions of PDD mcp gene on chromosome and dly gene on plasmid were selected as the target fragments to design the specific primers. Recombinant plasmid standard was prepared based on the primers. With GENECHECKER UF-150 qRT-PCR instrument as the platform, a fluorescence-based quantitative real-time PCR(qRT-PCR) method was established for the detection of PDD. This method can specifically detect PDD and distinguish the highly virulent strains. Additionally, the test results can be qualitatively judged by visualization, while the quantitative detection can be achieved through the standard curve calculation. The minimum limit of detection was 1.0×101 copies μL-1, and the detection time was less than 20 min. In summary, this new method has outstanding advantages, such as strong specificity, high sensitivity, and low site requirements. It is a rapid on-site detection technology for highly virulent PDD strains.
基金supported by the National Key R&D Programs of China(2022YFF0710701,2022YFF0710702)Guangzhou Key Research and Development Program(202206010157)+2 种基金Guangzhou Joint Fund for Key Laboratory(202201020381)Medical Joint Fund of Jinan University(YXJC202204)Open Research Project of the Key Laboratory of Viral Pathogenesis and Infection Prevention and Control of the Ministry of Education(2023VPPC-R02).
文摘Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.
文摘1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated from a 2 year old Bangladeshi child.长双歧杆菌幼儿亚种.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFD1200101)the Earmarked Fund for Modern Agro-industry Technology Research System(Grant No.CARS-23)Science and Technology Innovation Program of the Chinese Academy of Agricultural Science(Grant No.CAAS-ASTIP-IVFCAAS).
文摘Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in cucumber has not been reported.Here,we investigated the BSR resistance of 119 cucumber core germplasm worldwide at the seedling stage and identified 26 accessions highly resistant to BSR.A total of 1642740 single-nucleotide polymorphisms(SNPs)were used to conduct GWAS,and five loci associated with BSR resistance were detected on four chromosomes:gBSR2.1,gBSR2.2,gBSR3.1,gBSR4.1 and gBSR5.1.Based on haplotype analysis,sequence polymorphisms,functional annotation and qRT-PCR analysis,six candidate genes were identified within the five loci.CsaV3_2G014450,CsaV3_2G014490,CsaV3_2G016000,CsaV3_3G000850,CsaV3_4G033150,and CsaV3_5G000390 each had nonsynonymous SNPs,and were significantly up-regulated in the resistant genotypes after inoculation.And CsaV3_5G000390 in the susceptible genotype was significantly up-regulated after inoculation.The identification of these candidate genes lays a foundation for understanding the genetic mechanism of BSR resistance in cucumber.Generally,our study mined genes associated with BSR resistance in cucumber seedlings and will assist the breeding of BSR-resistant cucumber cultivars.
基金supported by the National Natural Science Foundation of China(32021005,32272332)the Fundamental Research Funds for the Central Universities(JUSRP622020,JUSRP51501)+1 种基金the Program of Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu ProvincePostgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX22_2391)。
文摘Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.
基金supported by the National Natural Science Foundation of China(32172211,32572557)the Key Science Research Projects of Higher Education Institutions in Henan Province(21A530006)+3 种基金the Key Research and Development and Promotion Projects of Henan Province(232102310293)the Henan Provincial Natural Science Foundation General Program(252300421403)the Zhongyuan Sci-Tech Innovation Leading Talents(254200510040)the Innovative Research Team(in Science and Technology)in University of Henan Province(26IRTSTHN030).
文摘Microbial food is an important direction of sustainable food development in the future.Microorganisms such as lactic acid bacteria(LAB)are important sources of natural radioprotectors.Moderate environmental stress can induce stress response and improve biological activity of LAB.In the previous study,Lactococcus lactis subsp.lactis IL1403(L.lactis IL1403)cell-free extract induced by ionizing radiation(IR)of 500 Gy(IR-CFE)presented the stronger radioprotective effect than untreated cell-free extract in mice.To explore the radioprotective active substances of IR-CFE,the key protein was screened by proteomics and its radioprotective effect in vivo was further evaluated.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment showed that two-component systems(TCS)were enhanced to adapt the IR induction.Meanwhile,the protein LlrG belonged to TCS was screened by the protein-protein interaction.Furthermore,the recombinant protein LlrG(rLlrG)could markedly alleviate the ^(60)Coγ-induced damage to the hematopoietic system,oxidative stress and inflammation in mice,thereby exerting its radioprotective function.These results suggested LlrG protein not only played an important role in the adaptation of L.lactis IL1403 to IR environment,but also exerted a good radioprotective effect,which could be applied in the development of protein-based radioprotectors.
基金funded by the National Key R&D Program of China(2023YFF1104501)R&D Program of Beijing Municipal Education Commission(23JF0006)National Natural Science Foundation of China(31601443 and 32101938)。
文摘Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs to be further investigated.This study aimed to examine the alleviating effect of Lacticaseibacillus paracasei L9 and Bifidobacterium animalis subsp.lactis A6 and possible mechanism in mice with AD.In this study,the administration of probiotics L9 and A6 effectively improved memory and learning functions in AD mice.The hippocampal cells of mice were partly recovered in morphology and rearranged more neatly after probiotics intervention.Meanwhile,L9 and A6 exhibited inhibitory effects on the phosphorylation of Tau and the deposition of Aβ,which were mediated via GSK-3βand PP2A kinases.Meanwhile,by metagenomic sequencing,we found interventions with L9 and A6 altered the intestinal microbiome's taxonomic composition,reduced the abundance of pathogenic Desulfovibrio genera,and increased beneficial Clostridium and Paramuribaculum genera abundance.The fatty acids metabolism and biosynthesis of gut microbiome were also enhanced.Serum untargeted metabolomics also showed noticeable alternation in lipidrelated metabolites,which may alleviate the pathogenesis of AD.These results revealed a mitigating role for probiotic L9 and A6 in AD prevention and offer new insights into AD prevention via gut-brain connection.
基金funded by the National Key R&D Program of China(2021YFD2100700)National Natural Science Foundation of China(32021005)+1 种基金111 project(BP0719028)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.
