Introduction:African wild olive,Olea europaea L.subsp.cuspidata(Wall.ex G.Don)Cif.,L‘Olivicoltore is widely distributed in dry forest and forest margins,often with Juniperus procera,in east Africa and Ethiopia.It rea...Introduction:African wild olive,Olea europaea L.subsp.cuspidata(Wall.ex G.Don)Cif.,L‘Olivicoltore is widely distributed in dry forest and forest margins,often with Juniperus procera,in east Africa and Ethiopia.It reaches southern Africa,also India and China,ranging from tall trees to stunted shrubs.Does best in good forest soil,but hardy and drought resistant once established,even in poor soils.It is used for firewood,charcoal,poles,posts,timber(furniture,carving,floors,and paneling),medicine(stem,bark,and leaves),bee forage,milk flavoring(smoking wood),toothbrushes(twigs),and walking sticks.Although the species has many economic and ecological functions,its environmental uses like carbon storage and climate change mitigation are less assessed.Therefore,the objective of the study was to develop species-specific allometric equations for O.europaea L.subsp.cuspidata using semi-destructive method and evaluate allometric models for estimating the aboveground biomass(AGB).Results and Discussions:After all the necessary biomass calculations were done,seven AGB equations were developed.These regression equations relate AGB with diameter at breast height(DBH),height(H),and density(ρ)individually and in combination.Out of seven,four allometric equations were chosen based on goodness-of-fit statistics and three were rejected.The selected models were tested for accuracy based on observed data.The best models selected have higher R2-adj and lower residual standard error and Akaike information criterion than rejected equations.The relations for all selectedmodels are significant(p<0.000),which showed strong correlation of AGB with selected dendrometric variables.Accordingly,the AGB was strongly correlated with DBH and was not significantly correlated with wood density and height individually in O.europaea L.subsp.cuspidata allometric equation development.In combination,AGB was strongly correlated with DBH and height;DBH and wood density;and the combination of DBH,height,and wood density.Species-specific equations are used for better carbon assessment than general equations.展开更多
为探究东北红豆杉对全球变暖的适应对策,基于稳定同位素技术,以不同生长阶段东北红豆杉(幼苗、幼树、成树)为研究对象,分析其叶片稳定碳同位素组成(δ^(13)C)、水分利用效率(water use efficiency,WUE)的季节动态及影响因子。结果表明,...为探究东北红豆杉对全球变暖的适应对策,基于稳定同位素技术,以不同生长阶段东北红豆杉(幼苗、幼树、成树)为研究对象,分析其叶片稳定碳同位素组成(δ^(13)C)、水分利用效率(water use efficiency,WUE)的季节动态及影响因子。结果表明,不同生长阶段东北红豆杉叶片δ^(13)C值变化范围为-3.051%~-2.939%,平均值为-2.981%±0.061%;WUE变化范围为58.96~71.68μmol/mol,平均值为66.87μmol/mol±6.90μmol/mol。东北红豆杉δ^(13)C值和WUE随季节变化由大到小排序为生长季前期(6月)、生长季中期(8月)、生长季后期(9月),不同生长阶段由大到小为成树、幼树、幼苗。不同生长阶段东北红豆杉WUE均与10 cm土壤含水量呈显著线性负相关(幼苗,y=-0.82x+107.29,R^(2)=0.80,P<0.01;幼树,y=-0.34x+84.17,R^(2)=0.45,P<0.05;成树,y=-0.93x+101.32,R^(2)=0.44,P<0.05),土壤含水量是东北红豆杉WUE的主控因子。不同生长阶段东北红豆杉根据植株个体水分需求及受外界水热因子影响程度选择不同的水分利用策略。展开更多
1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated fro...1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated from a 2 year old Bangladeshi child.长双歧杆菌幼儿亚种.展开更多
Taxus cuspidata,a rare species of the Taxus genus,and its wild resources are under severe threat.The development of cultivated species has become an important strategy to replace wild species.The objective of this wor...Taxus cuspidata,a rare species of the Taxus genus,and its wild resources are under severe threat.The development of cultivated species has become an important strategy to replace wild species.The objective of this work was to elucidate the differences in secondary metabolite accumulation,particularly in the paclitaxel biosynthesis pathway,between wild and cultivated species.This study employed liquid chromatography-tandem mass spectrometry(LC-MS/MS)and RNA sequencing(RNA-Seq)technologies to conduct integrated metabolomic and transcriptomic analyses of wild and cultivated species of T.cuspidata.The results showed that the content of paclitaxel in cultivated species was significantly higher than in wild species,reaching 1.67 times that of the latter(p 0.01).Additionally,the<content of key paclitaxel precursors,GGPP and 10-deacetylbaccatin III,in cultivated species was 1.94 times(p 0.05)<and 1.71 times(p 0.01)higher than in wild species,respectively.Transcriptomic analysis identified 2606 differentially<expressed genes(DEGs),among which key enzyme genes related to paclitaxel biosynthesis(such as DXS,DXR,GGPS,etc.)were generally upregulated in cultivated species.Multiple key enzyme genes in both the 2-C-methyl-D-erythritol 4-phosphate pathway(MEP)and paclitaxel biosynthesis pathways were significantly upregulated in cultivated species.Conversely,genes and metabolites related to sugar metabolism were found to be higher in content in wild species.These findings reveal the significant advantage of cultivated species in paclitaxel production capacity,providing new insights into the metabolic regulation mechanisms during yew domestication.This has important implications for optimizing paclitaxel biosynthesis and guiding future improvements in T.cuspidata cultivars.展开更多
Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in...Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in cucumber has not been reported.Here,we investigated the BSR resistance of 119 cucumber core germplasm worldwide at the seedling stage and identified 26 accessions highly resistant to BSR.A total of 1642740 single-nucleotide polymorphisms(SNPs)were used to conduct GWAS,and five loci associated with BSR resistance were detected on four chromosomes:gBSR2.1,gBSR2.2,gBSR3.1,gBSR4.1 and gBSR5.1.Based on haplotype analysis,sequence polymorphisms,functional annotation and qRT-PCR analysis,six candidate genes were identified within the five loci.CsaV3_2G014450,CsaV3_2G014490,CsaV3_2G016000,CsaV3_3G000850,CsaV3_4G033150,and CsaV3_5G000390 each had nonsynonymous SNPs,and were significantly up-regulated in the resistant genotypes after inoculation.And CsaV3_5G000390 in the susceptible genotype was significantly up-regulated after inoculation.The identification of these candidate genes lays a foundation for understanding the genetic mechanism of BSR resistance in cucumber.Generally,our study mined genes associated with BSR resistance in cucumber seedlings and will assist the breeding of BSR-resistant cucumber cultivars.展开更多
Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by g...Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.展开更多
Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effec...Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.展开更多
Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs t...Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs to be further investigated.This study aimed to examine the alleviating effect of Lacticaseibacillus paracasei L9 and Bifidobacterium animalis subsp.lactis A6 and possible mechanism in mice with AD.In this study,the administration of probiotics L9 and A6 effectively improved memory and learning functions in AD mice.The hippocampal cells of mice were partly recovered in morphology and rearranged more neatly after probiotics intervention.Meanwhile,L9 and A6 exhibited inhibitory effects on the phosphorylation of Tau and the deposition of Aβ,which were mediated via GSK-3βand PP2A kinases.Meanwhile,by metagenomic sequencing,we found interventions with L9 and A6 altered the intestinal microbiome's taxonomic composition,reduced the abundance of pathogenic Desulfovibrio genera,and increased beneficial Clostridium and Paramuribaculum genera abundance.The fatty acids metabolism and biosynthesis of gut microbiome were also enhanced.Serum untargeted metabolomics also showed noticeable alternation in lipidrelated metabolites,which may alleviate the pathogenesis of AD.These results revealed a mitigating role for probiotic L9 and A6 in AD prevention and offer new insights into AD prevention via gut-brain connection.展开更多
Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to ...Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.展开更多
文摘Introduction:African wild olive,Olea europaea L.subsp.cuspidata(Wall.ex G.Don)Cif.,L‘Olivicoltore is widely distributed in dry forest and forest margins,often with Juniperus procera,in east Africa and Ethiopia.It reaches southern Africa,also India and China,ranging from tall trees to stunted shrubs.Does best in good forest soil,but hardy and drought resistant once established,even in poor soils.It is used for firewood,charcoal,poles,posts,timber(furniture,carving,floors,and paneling),medicine(stem,bark,and leaves),bee forage,milk flavoring(smoking wood),toothbrushes(twigs),and walking sticks.Although the species has many economic and ecological functions,its environmental uses like carbon storage and climate change mitigation are less assessed.Therefore,the objective of the study was to develop species-specific allometric equations for O.europaea L.subsp.cuspidata using semi-destructive method and evaluate allometric models for estimating the aboveground biomass(AGB).Results and Discussions:After all the necessary biomass calculations were done,seven AGB equations were developed.These regression equations relate AGB with diameter at breast height(DBH),height(H),and density(ρ)individually and in combination.Out of seven,four allometric equations were chosen based on goodness-of-fit statistics and three were rejected.The selected models were tested for accuracy based on observed data.The best models selected have higher R2-adj and lower residual standard error and Akaike information criterion than rejected equations.The relations for all selectedmodels are significant(p<0.000),which showed strong correlation of AGB with selected dendrometric variables.Accordingly,the AGB was strongly correlated with DBH and was not significantly correlated with wood density and height individually in O.europaea L.subsp.cuspidata allometric equation development.In combination,AGB was strongly correlated with DBH and height;DBH and wood density;and the combination of DBH,height,and wood density.Species-specific equations are used for better carbon assessment than general equations.
文摘为探究东北红豆杉对全球变暖的适应对策,基于稳定同位素技术,以不同生长阶段东北红豆杉(幼苗、幼树、成树)为研究对象,分析其叶片稳定碳同位素组成(δ^(13)C)、水分利用效率(water use efficiency,WUE)的季节动态及影响因子。结果表明,不同生长阶段东北红豆杉叶片δ^(13)C值变化范围为-3.051%~-2.939%,平均值为-2.981%±0.061%;WUE变化范围为58.96~71.68μmol/mol,平均值为66.87μmol/mol±6.90μmol/mol。东北红豆杉δ^(13)C值和WUE随季节变化由大到小排序为生长季前期(6月)、生长季中期(8月)、生长季后期(9月),不同生长阶段由大到小为成树、幼树、幼苗。不同生长阶段东北红豆杉WUE均与10 cm土壤含水量呈显著线性负相关(幼苗,y=-0.82x+107.29,R^(2)=0.80,P<0.01;幼树,y=-0.34x+84.17,R^(2)=0.45,P<0.05;成树,y=-0.93x+101.32,R^(2)=0.44,P<0.05),土壤含水量是东北红豆杉WUE的主控因子。不同生长阶段东北红豆杉根据植株个体水分需求及受外界水热因子影响程度选择不同的水分利用策略。
文摘1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated from a 2 year old Bangladeshi child.长双歧杆菌幼儿亚种.
基金supported by grants from the National Science Foundation of China to Yanwen Zhang(32272757,31972363)grants from Liaoning Provincial Department of Education Project to Dandan Wang(JYTMS20230698)grants from the Liaoning Provincial Science and Technology.Fund Project to Dandan Wang(2023JH2/101700200).
文摘Taxus cuspidata,a rare species of the Taxus genus,and its wild resources are under severe threat.The development of cultivated species has become an important strategy to replace wild species.The objective of this work was to elucidate the differences in secondary metabolite accumulation,particularly in the paclitaxel biosynthesis pathway,between wild and cultivated species.This study employed liquid chromatography-tandem mass spectrometry(LC-MS/MS)and RNA sequencing(RNA-Seq)technologies to conduct integrated metabolomic and transcriptomic analyses of wild and cultivated species of T.cuspidata.The results showed that the content of paclitaxel in cultivated species was significantly higher than in wild species,reaching 1.67 times that of the latter(p 0.01).Additionally,the<content of key paclitaxel precursors,GGPP and 10-deacetylbaccatin III,in cultivated species was 1.94 times(p 0.05)<and 1.71 times(p 0.01)higher than in wild species,respectively.Transcriptomic analysis identified 2606 differentially<expressed genes(DEGs),among which key enzyme genes related to paclitaxel biosynthesis(such as DXS,DXR,GGPS,etc.)were generally upregulated in cultivated species.Multiple key enzyme genes in both the 2-C-methyl-D-erythritol 4-phosphate pathway(MEP)and paclitaxel biosynthesis pathways were significantly upregulated in cultivated species.Conversely,genes and metabolites related to sugar metabolism were found to be higher in content in wild species.These findings reveal the significant advantage of cultivated species in paclitaxel production capacity,providing new insights into the metabolic regulation mechanisms during yew domestication.This has important implications for optimizing paclitaxel biosynthesis and guiding future improvements in T.cuspidata cultivars.
基金supported by the National Key Research and Development Program of China(Grant No.2023YFD1200101)the Earmarked Fund for Modern Agro-industry Technology Research System(Grant No.CARS-23)Science and Technology Innovation Program of the Chinese Academy of Agricultural Science(Grant No.CAAS-ASTIP-IVFCAAS).
文摘Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in cucumber has not been reported.Here,we investigated the BSR resistance of 119 cucumber core germplasm worldwide at the seedling stage and identified 26 accessions highly resistant to BSR.A total of 1642740 single-nucleotide polymorphisms(SNPs)were used to conduct GWAS,and five loci associated with BSR resistance were detected on four chromosomes:gBSR2.1,gBSR2.2,gBSR3.1,gBSR4.1 and gBSR5.1.Based on haplotype analysis,sequence polymorphisms,functional annotation and qRT-PCR analysis,six candidate genes were identified within the five loci.CsaV3_2G014450,CsaV3_2G014490,CsaV3_2G016000,CsaV3_3G000850,CsaV3_4G033150,and CsaV3_5G000390 each had nonsynonymous SNPs,and were significantly up-regulated in the resistant genotypes after inoculation.And CsaV3_5G000390 in the susceptible genotype was significantly up-regulated after inoculation.The identification of these candidate genes lays a foundation for understanding the genetic mechanism of BSR resistance in cucumber.Generally,our study mined genes associated with BSR resistance in cucumber seedlings and will assist the breeding of BSR-resistant cucumber cultivars.
基金supported by the National Key R&D Programs of China(2022YFF0710701,2022YFF0710702)Guangzhou Key Research and Development Program(202206010157)+2 种基金Guangzhou Joint Fund for Key Laboratory(202201020381)Medical Joint Fund of Jinan University(YXJC202204)Open Research Project of the Key Laboratory of Viral Pathogenesis and Infection Prevention and Control of the Ministry of Education(2023VPPC-R02).
文摘Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.
基金supported by the National Natural Science Foundation of China(32021005,32272332)the Fundamental Research Funds for the Central Universities(JUSRP622020,JUSRP51501)+1 种基金the Program of Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu ProvincePostgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX22_2391)。
文摘Bifidobacterium longum subsp.infantis and Bifidobacterium adolescentis play important roles in the guts of infants and adolescents,respectively.In this study,using a neonatal rat model,we compared the protective effects of these 2 bifidobacterial species against Salmonella infection.The results demonstrated that B.longum subsp.infantis was more effective than B.adolescentis in alleviating the severity of infection in newborn rats exposed to Salmonella enterica serovar Typhimurium strain SL1344.B.longum subsp.infantis attenuated intestinal inflammation and mucosal damage induced by Salmonella infection,as well as protecting intestinal nerves and intestinal barrier function through TLR4/My D88 signalling.B.longum subsp.infantis also displayed the potential to modulate gut metabolites by promoting the biosynthesis of unsaturated fatty acids(arachidonic acid,eicosapentaenoic acid andα-linolenic acid)and purine metabolism(guanine,adenine,inosine and adenosine),thereby regulating metabolic disturbances.Additionally,the benefits of B.longum subsp.infantis were also observed in the liver,spleen and brain,improving nerve reflexes and suppressing hepatosplenomegaly.Overall,these findings provide novel insights into the prevention and treatment of gutrelated diseases in newborns,highlighting the potentially significant role of B.longum subsp.infantis in clinical applications.
基金funded by the National Key R&D Program of China(2023YFF1104501)R&D Program of Beijing Municipal Education Commission(23JF0006)National Natural Science Foundation of China(31601443 and 32101938)。
文摘Accumulating evidence suggests that probiotics affect the microbial-gut-brain axis in a way that can prevent and treat Alzheimer's disease(AD).However,the mechanism underlying the therapeutic effects still needs to be further investigated.This study aimed to examine the alleviating effect of Lacticaseibacillus paracasei L9 and Bifidobacterium animalis subsp.lactis A6 and possible mechanism in mice with AD.In this study,the administration of probiotics L9 and A6 effectively improved memory and learning functions in AD mice.The hippocampal cells of mice were partly recovered in morphology and rearranged more neatly after probiotics intervention.Meanwhile,L9 and A6 exhibited inhibitory effects on the phosphorylation of Tau and the deposition of Aβ,which were mediated via GSK-3βand PP2A kinases.Meanwhile,by metagenomic sequencing,we found interventions with L9 and A6 altered the intestinal microbiome's taxonomic composition,reduced the abundance of pathogenic Desulfovibrio genera,and increased beneficial Clostridium and Paramuribaculum genera abundance.The fatty acids metabolism and biosynthesis of gut microbiome were also enhanced.Serum untargeted metabolomics also showed noticeable alternation in lipidrelated metabolites,which may alleviate the pathogenesis of AD.These results revealed a mitigating role for probiotic L9 and A6 in AD prevention and offer new insights into AD prevention via gut-brain connection.
基金funded by the National Key R&D Program of China(2021YFD2100700)National Natural Science Foundation of China(32021005)+1 种基金111 project(BP0719028)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.
