Conchospores released from a wild-type strain of Pyropia suborbiculata were cultured in the laboratory for 20-80 days.Mother blades of different ages were enzymatically isolated to obtain single cells,which then devel...Conchospores released from a wild-type strain of Pyropia suborbiculata were cultured in the laboratory for 20-80 days.Mother blades of different ages were enzymatically isolated to obtain single cells,which then developed into different types of regenerative plants within a liquid medium.These regenerative plant types includes:normal blades,abnormal blades,cell-masses,and sexual cell-masses.The age of mother blades distinctly affected the number and proportion of each type of regenerative plants.When the age of mother blades increased from 20 to 80 days,the proportion of normal blades in the isolated cells sharply decreased,the percentage of abnormal blades increased,and the proportion of cell-masses was relatively unchanged.After 40 days of cultivation,sexual cell-masses appeared in regenerated plants derived from the single blade cells of the PS-WT strain,and gradually increased with mother cell age.In addition,the proportion of normal blades decreased in regenerated plants sourced from the basal to apical parts of the same alga,while the proportion of abnormal blades and cell-masses increased.In summary,the isolated cells of the gametophytic blade of P.suborbiculata developed into different types of regenerated plantlets in vivo due to the different stages of cell differentiation.We preliminarily concluded that the differentiation from conchospores to the sexual cells could be divided into at least seven different groups.展开更多
Several strains with long-type blade have been isolated from Pyropia suborbiculata,showing application potential for commercial Pyropia cultivation.In this study,using strain PS-M4 of P.suborbiculata,the effects of te...Several strains with long-type blade have been isolated from Pyropia suborbiculata,showing application potential for commercial Pyropia cultivation.In this study,using strain PS-M4 of P.suborbiculata,the effects of temperatures and light levels on conchocelis growth,conchosporangia formation,and conchospores release,were examined.The optimal conditions for conchocelis growth were 24℃ and 30-40μmol photons/(m^(2)⋅s),whereas the conchosporangia formation ratio peaked at 30℃ and 10-20μmol photons/(m^(2)⋅s),yielding the highest conchospore release under these conditions.Compared with strain PH-WT10 of P.haitanensis,the conchocelis of PS-M4 displayed higher thermotolerance and superior conchospore yield.These attributes underscore the suitability of PS-M4 for convenient and climate-resilient conchocelis cultivation.展开更多
基金The study was supported in part by the National Key Research and Development Program of China(2018YFD0900606)National Natural Science Foundation of China(31072208)+2 种基金Major Science and Technology Specific Program of Zhejiang Province(2016C02055-6)Science and Technology Planning Project of Jiangsu Province,China(BE2018335)Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province(2017fjscq02).
文摘Conchospores released from a wild-type strain of Pyropia suborbiculata were cultured in the laboratory for 20-80 days.Mother blades of different ages were enzymatically isolated to obtain single cells,which then developed into different types of regenerative plants within a liquid medium.These regenerative plant types includes:normal blades,abnormal blades,cell-masses,and sexual cell-masses.The age of mother blades distinctly affected the number and proportion of each type of regenerative plants.When the age of mother blades increased from 20 to 80 days,the proportion of normal blades in the isolated cells sharply decreased,the percentage of abnormal blades increased,and the proportion of cell-masses was relatively unchanged.After 40 days of cultivation,sexual cell-masses appeared in regenerated plants derived from the single blade cells of the PS-WT strain,and gradually increased with mother cell age.In addition,the proportion of normal blades decreased in regenerated plants sourced from the basal to apical parts of the same alga,while the proportion of abnormal blades and cell-masses increased.In summary,the isolated cells of the gametophytic blade of P.suborbiculata developed into different types of regenerated plantlets in vivo due to the different stages of cell differentiation.We preliminarily concluded that the differentiation from conchospores to the sexual cells could be divided into at least seven different groups.
基金supported by the National Key R&D Program of China(2023YFD2400105)the Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province(2022fjscq01).
文摘Several strains with long-type blade have been isolated from Pyropia suborbiculata,showing application potential for commercial Pyropia cultivation.In this study,using strain PS-M4 of P.suborbiculata,the effects of temperatures and light levels on conchocelis growth,conchosporangia formation,and conchospores release,were examined.The optimal conditions for conchocelis growth were 24℃ and 30-40μmol photons/(m^(2)⋅s),whereas the conchosporangia formation ratio peaked at 30℃ and 10-20μmol photons/(m^(2)⋅s),yielding the highest conchospore release under these conditions.Compared with strain PH-WT10 of P.haitanensis,the conchocelis of PS-M4 displayed higher thermotolerance and superior conchospore yield.These attributes underscore the suitability of PS-M4 for convenient and climate-resilient conchocelis cultivation.
