[Objectives] The aim was to study the chemical composition of the aboveground part of Artemisia subdigitata Mattf. [Methods]Using the systemic preparative test,the water extract,ethanol extract and petroleum ether ext...[Objectives] The aim was to study the chemical composition of the aboveground part of Artemisia subdigitata Mattf. [Methods]Using the systemic preparative test,the water extract,ethanol extract and petroleum ether extract of the aboveground part of A. subdigitata were subjected to physiochemical identification,and the contained chemical constituents were identified preliminarily. [Results] The preliminary comparisons of preparative test results of the aboveground part of A. subdigitata suggested that the whole plant may contain saponins,tannins,phenols,flavonoids,anthraquinones,coumarins,lactones,alkaloids,sterides or triterpenes,volatile oils and oils. [Conclusions]A. subdigitata contains a variety of chemical constituents. This study provides certain experimental basis for the further research and development and utilization of A. subdigitata.展开更多
Ten novel isocoumarins,including four pairs of enantiomers,were isolated from Artemisia dubia var.subdigitata(Asteraceae).Compounds 1,2 and 3a/3b possessed a unique 6/6/6-tricyclic system comprising an unusual 1-(2-me...Ten novel isocoumarins,including four pairs of enantiomers,were isolated from Artemisia dubia var.subdigitata(Asteraceae).Compounds 1,2 and 3a/3b possessed a unique 6/6/6-tricyclic system comprising an unusual 1-(2-methylcyclohexyl)propan-1-one moiety fused with isocoumarin core skeleton.Compounds 4a/4b were characterized as an unexpected 2,5-dimethylcyclohexan-1-one scaffold,and compounds 5a/5b and 6a/6b were rare 1,2-seco-isocoumarin.Their structures and absolute configurations were elucidated through spectroscopic data,X-ray crystallography,ECD and NMR calculations with DP4+analyses.Plausible biosynthetic pathways were proposed from the naturally occurring isocoumarin.Network pharmacological analysis suggested that the targets of compound 1 were significantly enriched in the cell cycle and Pl3K-Akt signaling pathway.The molecular docking revealed that compound 1 had high binding affinity with CDK2(total score:6.8717).Furthermore,compounds 1 and 2 exhibited inhibitory activity on three human hepatoma cell lines,with inhibitory ratios of 85.1% and 84.5%(HepG2),88.2% and 87.3%(Huh7),and 69.2% and 69.1%(SK-Hep-1)at 200μmol·L^(-1),respectively.展开更多
基金Supported by National Key Technology Research and Development Plan(2015BAC05B02)Fundamental Research Funds for the Central Universities(2018NZD10)
文摘[Objectives] The aim was to study the chemical composition of the aboveground part of Artemisia subdigitata Mattf. [Methods]Using the systemic preparative test,the water extract,ethanol extract and petroleum ether extract of the aboveground part of A. subdigitata were subjected to physiochemical identification,and the contained chemical constituents were identified preliminarily. [Results] The preliminary comparisons of preparative test results of the aboveground part of A. subdigitata suggested that the whole plant may contain saponins,tannins,phenols,flavonoids,anthraquinones,coumarins,lactones,alkaloids,sterides or triterpenes,volatile oils and oils. [Conclusions]A. subdigitata contains a variety of chemical constituents. This study provides certain experimental basis for the further research and development and utilization of A. subdigitata.
基金financially supported by the Key Program of the National Natural Science Foundation of China(22137008)the Xingdian Yingcai Project(YNWR-KJLJ-2019-002)+1 种基金the Youth Innovation Promotion Association,CAs(2020386)the Reserve Talents of Young and Middle-aged Academic and Technical Leaders in Yunnan Province(202105AC160021).
文摘Ten novel isocoumarins,including four pairs of enantiomers,were isolated from Artemisia dubia var.subdigitata(Asteraceae).Compounds 1,2 and 3a/3b possessed a unique 6/6/6-tricyclic system comprising an unusual 1-(2-methylcyclohexyl)propan-1-one moiety fused with isocoumarin core skeleton.Compounds 4a/4b were characterized as an unexpected 2,5-dimethylcyclohexan-1-one scaffold,and compounds 5a/5b and 6a/6b were rare 1,2-seco-isocoumarin.Their structures and absolute configurations were elucidated through spectroscopic data,X-ray crystallography,ECD and NMR calculations with DP4+analyses.Plausible biosynthetic pathways were proposed from the naturally occurring isocoumarin.Network pharmacological analysis suggested that the targets of compound 1 were significantly enriched in the cell cycle and Pl3K-Akt signaling pathway.The molecular docking revealed that compound 1 had high binding affinity with CDK2(total score:6.8717).Furthermore,compounds 1 and 2 exhibited inhibitory activity on three human hepatoma cell lines,with inhibitory ratios of 85.1% and 84.5%(HepG2),88.2% and 87.3%(Huh7),and 69.2% and 69.1%(SK-Hep-1)at 200μmol·L^(-1),respectively.
