In the past decade,there has been increasing interest in the applications of cyclometalated iridium(Ⅲ)polypyridine complexes in different areas.Since many of these complexes exhibit interesting photophysical properti...In the past decade,there has been increasing interest in the applications of cyclometalated iridium(Ⅲ)polypyridine complexes in different areas.Since many of these complexes exhibit interesting photophysical properties,they have been utilized in the development of luminescent chemosensors,light-emitting devices,and photovoltaic cells.In addition,a number of organometallic iridium(Ⅲ)polypyridine complexes have been applied in a range of biological studies.In this article,we summarize the applications of a selection of these complexes with a focus on intracellular sensors,organelle-targeting imaging reagents,and metallodrugs.展开更多
Synchrotron Radiation X-ray Fluorescence(SXRF)imaging is a powerful technique for the visualization of metal complexes in biological systems.However,due to the lack of an endogenous elemental signature for mitochondri...Synchrotron Radiation X-ray Fluorescence(SXRF)imaging is a powerful technique for the visualization of metal complexes in biological systems.However,due to the lack of an endogenous elemental signature for mitochondria,probes for the localization of this organelle are required for colocalization studies.In this work,we designed and synthesized rhenium pyta tricarbonyl complexes conjugated to methylated triphenylphosphonium TP^(*)P^(+)cations as multimodal probes for the visualization of mitochondria,suitable for fluorescence and SXRF imaging and quantification.Accumulation of the methylated triphenylphosphonium TP^(*)P^(+)-based conjugates in cells was observed in fixed A549 cells,and the amount of mitochondrial uptake was linked to the lipophilicity of the TPP+vector.Our work highlights a convenient rhenium-based multimodal mitochondrial-targeted probe compatible with SXRF nano-imaging.展开更多
基金City University of Hong Kong(Project no.7004210 and 9667090)for financial supportthe Croucher Foundation for a Croucher Senior Research Fellowshipthe receipt of a Postgraduate Studentship administered by City University of Hong Kong.
文摘In the past decade,there has been increasing interest in the applications of cyclometalated iridium(Ⅲ)polypyridine complexes in different areas.Since many of these complexes exhibit interesting photophysical properties,they have been utilized in the development of luminescent chemosensors,light-emitting devices,and photovoltaic cells.In addition,a number of organometallic iridium(Ⅲ)polypyridine complexes have been applied in a range of biological studies.In this article,we summarize the applications of a selection of these complexes with a focus on intracellular sensors,organelle-targeting imaging reagents,and metallodrugs.
基金the SOLEIL committee for beamtime(project 20190252)members from Nanoscopium Beamline.CNRS is acknowledged for H.B.delegation to Singapore and H.B.thanks W.K.Leong for hosting her in his group and for his support during this period+4 种基金the Fondation pour la Recherche Médicale for financial support(contract DIE20151234413call“Pionniers de la recherche,Etudes physicochimiques innovantes pour la biologie et la médecine)Institut Curie(UMR9187,F.Poyer and F.Mahuteau-Betzer)for providing A549 cellsZ.Gueroui(ENS chemistry department)for useful discussions and help with fluorescence microscopy.F.G.would like to thank A*STAR AME IRG A1783c0003NTU for a start-up grant(M4080552)and MOE Tier 1 grants(RG 11/15 and RG 113/16)for financial support.H.C.would like to thank NTU for NPGS scholarship.
文摘Synchrotron Radiation X-ray Fluorescence(SXRF)imaging is a powerful technique for the visualization of metal complexes in biological systems.However,due to the lack of an endogenous elemental signature for mitochondria,probes for the localization of this organelle are required for colocalization studies.In this work,we designed and synthesized rhenium pyta tricarbonyl complexes conjugated to methylated triphenylphosphonium TP^(*)P^(+)cations as multimodal probes for the visualization of mitochondria,suitable for fluorescence and SXRF imaging and quantification.Accumulation of the methylated triphenylphosphonium TP^(*)P^(+)-based conjugates in cells was observed in fixed A549 cells,and the amount of mitochondrial uptake was linked to the lipophilicity of the TPP+vector.Our work highlights a convenient rhenium-based multimodal mitochondrial-targeted probe compatible with SXRF nano-imaging.
