Dianthus spiculifolius Schur,as an emerging ornamental plant,has extensive applications and economic values.In this study,the DsCBL4 gene was successfully cloned,and its tissue-specific expression,expression patterns ...Dianthus spiculifolius Schur,as an emerging ornamental plant,has extensive applications and economic values.In this study,the DsCBL4 gene was successfully cloned,and its tissue-specific expression,expression patterns under various abiotic stresses,subcellular localization,and bioinformatics analysis of the encoded amino acid sequence were conducted.The results showed that the coding region of the DsCBL4 gene was 675 bp long,encoding 224 amino acids.It had high homology with the amino acids encoded by Amaranthus tricolor,Chenopodium quinoa and Spinacia oleracea.The predicted relative molecular mass of DsCBL4 was 25.61 ku,with an isoelectric point of 4.58,and it had phosphorylation sites,belonging to an unstable hydrophilic protein.Its secondary structure includedα-helices,irregular coils and extended chains.The tertiary structure prediction revealed that DsCBL4 had four EFhand calcium-binding domains necessary for Ca2+binding in plant calmodulin-like proteins and the FPSF motif for calcineurin B-like protein(CBL)-interacting protein kinase(CIPK)activation.The expression level of the DsCBL4 gene showed tissue specificity,with the highest expression in roots.It was induced by drought,low temperature,combined drought and low temperature,salt stress,nitrogen stress,phosphorus stress,calcium ion stress,high temperature stress,and abscisic acid(ABA)stress.Both transient infection in Nicotiana tabacum L.and stable expression in transgenic Arabidopsis thaliana showed that the DsCBL4 protein was localized to the cell membrane.These results suggested that DsCBL4 might be involved in the abiotic stress response of Dianthus spiculifolius through the calcium signaling pathway,providing a theoretical basis for understanding its molecular mechanism.This study provided an important reference for further exploring the role of the DsCBLs gene family in plant stress resistance.展开更多
Neurodegenerative disorders,including Alzheimer’s disease(AD),Parkinson’s disease(PD),and amyotrophic lateral sclerosis,impose a considerable social and economic burden on society and have dramatic consequences for ...Neurodegenerative disorders,including Alzheimer’s disease(AD),Parkinson’s disease(PD),and amyotrophic lateral sclerosis,impose a considerable social and economic burden on society and have dramatic consequences for individuals and their families.The majority of existing interventions have been found to be capable of only a slight modification of disease progression or to moderately delay significant functional decline in motor,cognitive,or mental domains.展开更多
Clustering analysis identifying unknown heterogenous subgroups of a population(or a sample)has become increasingly popular along with the popularity of machine learning techniques.Although there are many software pack...Clustering analysis identifying unknown heterogenous subgroups of a population(or a sample)has become increasingly popular along with the popularity of machine learning techniques.Although there are many software packages running clustering analysis,there is a lack of packages conducting clustering analysis within a structural equation modeling framework.The package,gscaLCA which is implemented in the R statistical computing environment,was developed for conducting clustering analysis and has been extended to a latent variable modeling.More specifically,by applying both fuzzy clustering(FC)algorithm and generalized structured component analysis(GSCA),the package gscaLCA computes membership prevalence and item response probabilities as posterior probabilities,which is applicable in mixture modeling such as latent class analysis in statistics.As a hybrid model between data clustering in classifications and model-based mixture modeling approach,fuzzy clusterwise GSCA,denoted as gscaLCA,encompasses many advantages from both methods:(1)soft partitioning from FC and(2)efficiency in estimating model parameters with bootstrap method via resolution of global optimization problem from GSCA.The main function,gscaLCA,works for both binary and ordered categorical variables.In addition,gscaLCA can be used for latent class regression as well.Visualization of profiles of latent classes based on the posterior probabilities is also available in the package gscaLCA.This paper contributes to providing a methodological tool,gscaLCA that applied researchers such as social scientists and medical researchers can apply clustering analysis in their research.展开更多
Infrared(IR)spectroscopy,a technique within the realm of molecular vibrational spectroscopy,furnishes distinctive chemical signatures pivotal for both structural analysis and compound identification.A notable challeng...Infrared(IR)spectroscopy,a technique within the realm of molecular vibrational spectroscopy,furnishes distinctive chemical signatures pivotal for both structural analysis and compound identification.A notable challenge emerges from the misalignment between the mid-IR light wavelength range and molecular dimensions,culminating in a constrained absorption cross-section and diminished vibrational absorption coefficients(Supplementary data).展开更多
In the structural reliability analysis,the first-order reliability method(FORM)often yields significant errors when addressing nonlinear problems.Although the second-order reliability method(SORM)can provide higher ac...In the structural reliability analysis,the first-order reliability method(FORM)often yields significant errors when addressing nonlinear problems.Although the second-order reliability method(SORM)can provide higher accuracy,the additional computation of the Hessian matrix leads to lower computational efficiency.Additionally,when the dimensionality of the random variables is high,the approximation formula of SORM can result in larger errors.To address these issues,a structural reliability analysis method based on Kriging and spherical cap area integral is proposed.Firstly,this method integrates FORM with the quasi-Newton algorithm Broyden-Fletcher-Goldfarb-Shanno(BFGS),trains the Kriging model by using sample points from the algorithm’s iteration process,and combines the Kriging model with gradient information to approximate the Hessian matrix.Then,the failure surface is approximated as a rotating paraboloid,utilizing the spherical cap to replace the complex surface.For the n-dimensional case,the hyperspherical cap area expression is combined with the integral method to calculate the failure probability.Finally,the method is validated through three examples,demonstrating improved computational accuracy and efficiency compared to traditional methods.展开更多
[Objective] The study was to analyze the structure and function of HpaGXoo and the relationship between the two.[Method] Some related bioinformatics analysis software on internet such as NPSA,Swiss-Model,SAPS and Inte...[Objective] The study was to analyze the structure and function of HpaGXoo and the relationship between the two.[Method] Some related bioinformatics analysis software on internet such as NPSA,Swiss-Model,SAPS and InterPro Scan were adopted to analyze the structure and predict its function.[Result] HpaGXoo consists of 139 amino acids,and has many alpha-helical and coiled structure,no signal peptide on N-terminal and no transmembrane structure.It locates in bacterial cytoplasm.[Conclusion] The study will lay ...展开更多
A detailed understanding of seismicity originating from the Nanga Parbat syntaxis in the northwestern Himalaya is crucial for characterizing the active fault systems and associated neotectonic processes in the region....A detailed understanding of seismicity originating from the Nanga Parbat syntaxis in the northwestern Himalaya is crucial for characterizing the active fault systems and associated neotectonic processes in the region.Continuous earthquake monitoring through local seismic stations enables high-precision results by constraining the velocity structure.In this study,seismogram data from 244 small-magnitude earthquakes are analyzed to delineate the crustal thickness and investigate the source mechanisms beneath the Nanga Parbat syntaxis.The results are achieved with the application of Coupled Hypocenter Velocity Inversion(CHVI)analysis and Time Domain Moment Tensor(TDMT)analysis.The velocity inversion suggests that the Moho discontinuity lies at 60 km depth with an average vP/vS ratio of 1.735±0.017.The minimum 1D velocity model obtained through velocity inversion with least RMS error is further utilized in determining the source mechanism solution.In contrast to earlier studies,which highlighted strike-slip displacement accompanied by reverse dip-slip components,the present research provides a revised interpretation.The moment tensor analysis conducted in this study provides evidence of transtensional deformation associated with neotectonics,attributed to the presence of multiple shear zones.The results of the source mechanism for the selected earthquakes unveiled that the oblique-slip deformation is significantly controlled by the shear stresses coupled with the normal component of dip-slip movement.This is further supported by the higher values of the doublecouple moment tensor(85%),which indicate shear deformation,while the positive value of the compensated linear vector dipole(15%)confirms the presence of a normal component.The coexistence of transpressive and transtensive stresses,together with shallow hypocentral depths and high-amplitude tangential waveforms,can potentially cause devastating impacts in the surroundings of the Nanga Parbat syntaxis.展开更多
O3-types layered cathode materials in sodium-ion batteries(SIBs)suffer from the obvious lattice distortion induced by the complex phase transitions during Na^(+)intercalation/deintercalation process,leading to severe ...O3-types layered cathode materials in sodium-ion batteries(SIBs)suffer from the obvious lattice distortion induced by the complex phase transitions during Na^(+)intercalation/deintercalation process,leading to severe structural collapse and performance degradation.Herein,a series of high valence tantalum(Ta^(5+))doped Na(Ni_(0.4)Fe_(0.2)Mn_(0.4))_(1−x)Ta_(x)O_(2)(x=0/0.0025/0.005/0.01)secondary spherical particles are firstly developed,where Ta^(5+)doping enables the refined primary grain with a tightly stacked rod-like morphology.Comprehensive structural analysis via Neutron powder diffraction(NPD)and Synchrotron radiation X-ray diffraction(SXRD)reveals an expanded NaO_(2)slab and a reduction in Na site vacancy.The potential charge compensation mechanism is further illustrated by X-ray absorption spectroscopy(XAS)and X-ray photoelectron spectroscopy(XPS),unveiling a partial reduction from Ni^(3+)to Ni^(2+)with Ta^(5+)doping.In situ X-ray diffraction(in situ XRD)suggests that the decorated sample undergoes a volume change as low as 0.8%,in contrast with the pristine one(1.5%).Thus,the optimized sample with x=0.005 retains an enhanced capacity retention up to 70.4%at 1 C after 300 cycles in half-cell and delivers a high energy density of 251 Wh kg^(-1)(0.1 C)and with a good capacity retention of 81.0%at 1 C after 200 cycles in full-cell.Our findings provide new insights into the mechanism of high valence Ta^(5+)doping in stabilizing layered oxides cathode materials for SIBs.展开更多
Water-soluble crude polyseccharide（PIP） was extracted from cultured mycelium of the fungus Phellinus igniarius. After ethanol precipitation and sepharose CL-6B gel filtration, the fraction of PIP1 was obtained, whic...Water-soluble crude polyseccharide（PIP） was extracted from cultured mycelium of the fungus Phellinus igniarius. After ethanol precipitation and sepharose CL-6B gel filtration, the fraction of PIP1 was obtained, which was shown to be a homogeneous polysaccharide by means of high-performance liquid chromatography. The structure of PIPt was determined by using several methods. C.,C analysis indicates that PIP1 is composed of the monosaccharides of glucose, galactose, and mannose. Their malar ratio is 3. 70： 4. 06： 1.00. The molar weight was estimated to be 17 kd via HPLC. IR, GC, partial hydrolysis with acid, pefiedate oxidation, Smith degradation, methylation, and GC-MS analysis were used for the structural analyses of PIP1. The results show that PIP1 has a small quantity of branch structure, The main glycosidic linkage of PIP1 has a β-configurafion. The main chain is made up of a large mass of glucose （ 1→3 ） and few mannose （ 1→4 ） ; the side chain is composed of glucose （ 1 →3 ） and galactose （ 1→6 ） ; the nonreduced end is composed of galactose and glucose. The side chains are branched at 6-0 of glucose（ 1→3,6） and mannose（1→4,6）. On an average, there are three branches among 20 residues. It is presumable that the existence of 1,3-linked Glc in the main and side chains is the main reason for its higher antitumor activity.展开更多
An optimization method for the consistent evaluation of two Rayleigh damping coefficients is proposed. By minimizing an objective function such as an error term of the peak displacement of a structure, the two coeffic...An optimization method for the consistent evaluation of two Rayleigh damping coefficients is proposed. By minimizing an objective function such as an error term of the peak displacement of a structure, the two coefficients can be determined with response spectral analysis. The optimization method degenerates into the conventional method used in current practices when only two modes of vibration are included in the objective function. Therefore, the proposed method with all significant modes included for simplicity in practical applications results in suboptimal damping coefficients. The effects of both spatial distribution and frequency content of excitations as well as structural dynamic characteristics on the evaluation of Rayleigh damping coefficients were investigated with a five-story building structure. Two application examples with a 62-story high-rise building and a 840 m long cable-stayed bridge under ten earthquake excitations demonstrated the accuracy and effectiveness of the proposed method to account for all of the above effects.展开更多
Conventional element based methods for modeling acoustic problems are limited to low-frequency applications due to the huge computational efforts.For high-frequency applications,probabilistic techniques,such as statis...Conventional element based methods for modeling acoustic problems are limited to low-frequency applications due to the huge computational efforts.For high-frequency applications,probabilistic techniques,such as statistical energy analysis(SEA),are used.For mid-frequency range,currently no adequate and mature simulation methods exist.Recently,wave based method has been developed which is based on the indirect TREFFTZ approach and has shown to be able to tackle problems in the mid-frequency range.In contrast with the element based methods,no discretization is required.A sufficient,but not necessary,condition for convergence of this method is that the acoustic problem domain is convex.Non-convex domains have to be partitioned into a number of(convex)subdomains.At the interfaces between subdomains,specific coupling conditions have to be imposed.The considered two-dimensional coupled vibro-acoustic problem illustrates the beneficial convergence rate of the proposed wave based prediction technique with high accuracy.The results show the new technique can be applied up to much higher frequencies.展开更多
Spot weld models are widely used in finite element analysis(FEA)of automotive body in white(BIW)to predict static,dynamic,durability and other characteristics of automotive BIW.However,few researches are done on evalu...Spot weld models are widely used in finite element analysis(FEA)of automotive body in white(BIW)to predict static,dynamic,durability and other characteristics of automotive BIW.However,few researches are done on evaluation of the validity of these spot weld models in structural dynamic analysis of BIW.To evaluate the validity and accuracy of spot weld models in structural dynamic analysis of BIW,two object functions,error function and deviation function,are introduced innovatively.Modal analysis of Two-panel and Double-hat structures,which are the dominated structures in BIW,is conducted,and the values of these two object functions are obtained.Based on the values of object functions,the validity of these spot weld models are evaluated.It is found that the area contact method(ACM2)and weld element connection(CWELD)can give more precise prediction in modal analysis of these two classical structures,thus are more applicable to structural dynamic analysis of automotive BIW.Modal analysis of a classical BIW is performed,which further confirms this evaluation.The error function and deviation function proposed in this research can give guidance on the adaptability of spot weld models in structural dynamic analysis of BIW.And this evaluation method can also be adopted in evaluation of other finite element models in static,dynamic and other kinds of analysis for automotive structures.展开更多
With great superiorities in energy density,rate capability and structural stability,Na_(3)V_(2)(PO_(4))_(2) F_(3)(NVPF)has attracted much attentions as cathode of sodium ion battery(SIB),but it also faces challenges o...With great superiorities in energy density,rate capability and structural stability,Na_(3)V_(2)(PO_(4))_(2) F_(3)(NVPF)has attracted much attentions as cathode of sodium ion battery(SIB),but it also faces challenges on its poor intrinsic electronic conductivity and the controversial de/sodiation mechanism.Herein,a series of Zr-doped NVPF coated by N-doped carbon layer(~5 nm in thickness,homogenously)materials are fabricated by a sol-gel method,and the optimized heteroatom-doping amounts of Zr and N doping improve intrinsic properties on enlarging lattice distance and enhancing electronic conductivity,respectively.Specifically,among all samples of Na_(3) V_(2-x)Zr_(x)(PO_(4))_(2) F_(3)/NC(NVPF-Zr-x/NC,x=0,0.01,0.02,0.05,and 0.1),the optimized electrode of NVPF-Zr-0.02/NC delivers high reversible capacities(119.2 mAh g^(-1) at0.5 C),superior rate capability(98.1 mA h g^(-1) at 20 C)and excellent cycling performance.The structural evolution of NVPF-Zr-0.02/NC electrode,in-situ monitored by X-ray diffractometer,follows a step-wise Na-extraction/intercalation mechanism with reversible multi-phase changes,not just a solid-solutionreaction one.Full cells of NVPF-Zr-0.02/NC//hard carbon demonstrate high capacity(99.8 mA h g^(-1) at 0.5 C),high out-put voltage(3.5 V)and good cycling stability.This work is favorable to accelerate the development of high-performance cathode materials and explore possible redox reaction mechanisms of SIBs.展开更多
The North Qilian Shan fold and thrust belt,located at the northern Tibetan Plateau and southern margin of the Hexi Corridor,is a key tectonic unit to decode the formation and expansion of the plateau.Previous studies ...The North Qilian Shan fold and thrust belt,located at the northern Tibetan Plateau and southern margin of the Hexi Corridor,is a key tectonic unit to decode the formation and expansion of the plateau.Previous studies emphasize the Cenozoic deformation due to the far-field response to the Indo-Asian collision,but the Mesozoic deformations are poorly constrained in this area.We conducted detailed field mapping,structural analysis,geochronology,and structural interpretation of deep seismic reflectional profiling and magnetotelluric(MT)sounding,to address the superposed results of the Mesozoic and Cenozoic deformation.The results recognized the North Qilian thrust and nappe system(NQTS),the root and the frontal belt are the North Qilian thrust(NQT),and the Yumu Shan klippe(YK),respectively.The middle belt is located between the NQT and the YK.Monzonitic granite zircon U-Pb dating from the middle belt yields an age of ca.415 Ma,which is similar to south NQT.The thrusting displacement is estimated at ca.48 km by structural interpretation of deep profiles.The timing is constrained in the early stage of the Early Cretaceous by the formation of simultaneous growth strata.We suggest that the NQTS has resulted from the far-field effect of the Lhasa-Qiangtang collision,and the Yumu Shan is uplifted by the superposed Cenozoic deformation.展开更多
To better understand the genetic diversity and population structure of broccoli cultivars planted in China,a total of 161 representative broccoli cultivars in the past 25 years were collected and analysed based on sin...To better understand the genetic diversity and population structure of broccoli cultivars planted in China,a total of 161 representative broccoli cultivars in the past 25 years were collected and analysed based on single nucleotide polymorphism(SNP)markers.Ten pairs of primers with good polymorphism and high resolution were screened from 315 pairs of SNP primers by 3 broccoli accessions(inbred lines)with different phenotypes and maturity.The 10 pairs of SNP primers were selected,producing 78 alleles.The diversity analysis indicated that the polymorphism information content(PIC)of SNP primer ranged from 0.64 to 0.90.The observed number of alleles(Na)was 2.00,the effective number of alleles(Ne)was 1.11–2.00,the Nei’s gene diversity(H)was 0.10–0.50,and Shannon information index(I)was 0.20–0.70 using PopGene32 software.The clustering results showed that the 161 broccoli cultivars could be divided into 4 major subgroups(A,B,C and D),foreign cultivars were all assigned to subgroup A,and domestic cultivars were assigned to 3 subgroups of B,C,and D.This study indicated that some domestic cultivars and foreign cultivars were similar in genetic background,but most domestic cultivars were still different from the Japanese cultivars.When K=2,the population structure result presented that 161 broccoli cultivars could be divided into 1 simple group(2 groups)and 1 mixed group.When Q≥0.6,143(88.82%)broccoli cultivars belonged to the simple groups.In simple groups 68(42.24%)broccoli cultivars of group 1 were derived from Japan,the United States,Switzerland,the Netherlands,China-Taiwan,and China-Mainland;75(46.58%)broccoli cultivars belonged to group 2;when Q<0.6,18(11.18%)broccoli cultivars belonged to the mixed groups.This study is helpful to understand the diversity and resolution of broccoli cultivars from worldwide,which is beneficial to plant breeding and materials innovation.And meanwhile,this result is also used for construction of broccoli fingerprint serving for cultivar identification.展开更多
According to the hydraulic calculation principles of the orifice outflow, the discharge capacity of the columnar reversing gate under the partial opening condition was calculated and checked. Using ANSYS, a large fini...According to the hydraulic calculation principles of the orifice outflow, the discharge capacity of the columnar reversing gate under the partial opening condition was calculated and checked. Using ANSYS, a large finite element analysis software, the discharge process was simulated. The distribution rule of the velocities in the gate chamber and downstream channel was obtained. An FEM model of the columnar reversing gate was built, and the natural vibration properties of the gate were analyzed. Based on the Westergaard added mass method, the added mass caused by the fluid-structure coupling motion was taken into account, and the effects of the coupling interaction were discussed. The results show that the size of the small gates meets the demand for discharge capacity, the current in the gate chamber is quite turbulent, the trunnion and arms are obviously impacted by flow, and the effects of water on vibration characteristics are remarkable. The study provides a reference for the design and calculation of gates of the same type.展开更多
Thirteen fractions from the pathogenic plant fungus Setosphaeria turcica race 1 were separated and collected using high performance liquid chromatography （HPLC）. Their toxic activities were assayed through leaf punc...Thirteen fractions from the pathogenic plant fungus Setosphaeria turcica race 1 were separated and collected using high performance liquid chromatography （HPLC）. Their toxic activities were assayed through leaf puncturing on corn differentials （OH43, OH43Ht1, OH43Ht2, and OH43HtN）, and the results revealed that eight fractions were toxic and fraction 6 was specifically toxic to OH43Ht1, which could be taken as a gene-selective toxin fraction. Fraction 6 was finely purified via HPLC and condensed by freeze desiccation. Its chemical structure was analyzed with EI-MS, IR, HMBC, ^1H-NMR, and two-dimensional NMR. The results suggested that fraction 6 contained an unsaturated double bond, carbonyl and methylene groups with molecular weight of 142.展开更多
Compressional region usually forms complex thrust faults system, which is difficult to identify using traditional migration profiles. The successful application of three-dimensional（3D） seismic attributes analysis g...Compressional region usually forms complex thrust faults system, which is difficult to identify using traditional migration profiles. The successful application of three-dimensional（3D） seismic attributes analysis greatly reduces the difficulty, and improves the accuracy and efficiency of seismic interpretation and structural analysis. In this paper, we took Qiongxi area in the compressional region of western Sichuan as an example, using two 3D seismic attributes, coherence and instantaneous phase, to identify fault assemblages and variations both vertically and laterally. The results show that the study area mainly consists of NS-, NE- and NEE-trending faults. The NS-trending faults are the largest and have a component of sinistral slip controlling the formation of NEE-trending faults, while the NE-trending faults are intermediate in scale, formed earlier and were cut by the NS-trending faults. Our results demonstrate that using seismic attributes for structural analysis have the following advantages：（1） more details of major fault zones,（2） highlighting minor faults which are hardly traced in seismic migration cube, and（3） easier acquisition of accurate fault systems. The application of seismic attributes provides a new idea for deciphering fine and complicated structures, and will significantly contribute to the development of objective and precise geological interpretation in the future.展开更多
In the tubular frames with simple joints, the joints may show considerable local flexibility, which causes excessive deformation and affects internal force distributions. It is useful and important to study turbular j...In the tubular frames with simple joints, the joints may show considerable local flexibility, which causes excessive deformation and affects internal force distributions. It is useful and important to study turbular joint flexibility as well as the effect of joint local flexibility on the global behavior of tubular structure for the design and building of offshore platforms. A new method for computing the stiffness coefficients of simple tubular joints is developed in this paper based on two previous computing models. According to this method, the local flexibility of T, Y, TY tubular joints are computed. This method has merits of clear physical sense, simplicity of computations and less c.p.u. time. The effect of flexibility of tubular joints on the global behavior of framed tubular structures is investigated by comparing the computed results of tubular structures with flexible tubular joints with those with rigid connections. The comparison shows that the consideration of local flexibility of tubular joints and its effect on the stress analysis of offshore structures has its practical significance.展开更多
基金Supported by the National Key R&D Program(2022YFF1300500)the Spring Goose Support Program(CYQN24018)the National Natural Science Foundation of China(32572123)。
文摘Dianthus spiculifolius Schur,as an emerging ornamental plant,has extensive applications and economic values.In this study,the DsCBL4 gene was successfully cloned,and its tissue-specific expression,expression patterns under various abiotic stresses,subcellular localization,and bioinformatics analysis of the encoded amino acid sequence were conducted.The results showed that the coding region of the DsCBL4 gene was 675 bp long,encoding 224 amino acids.It had high homology with the amino acids encoded by Amaranthus tricolor,Chenopodium quinoa and Spinacia oleracea.The predicted relative molecular mass of DsCBL4 was 25.61 ku,with an isoelectric point of 4.58,and it had phosphorylation sites,belonging to an unstable hydrophilic protein.Its secondary structure includedα-helices,irregular coils and extended chains.The tertiary structure prediction revealed that DsCBL4 had four EFhand calcium-binding domains necessary for Ca2+binding in plant calmodulin-like proteins and the FPSF motif for calcineurin B-like protein(CBL)-interacting protein kinase(CIPK)activation.The expression level of the DsCBL4 gene showed tissue specificity,with the highest expression in roots.It was induced by drought,low temperature,combined drought and low temperature,salt stress,nitrogen stress,phosphorus stress,calcium ion stress,high temperature stress,and abscisic acid(ABA)stress.Both transient infection in Nicotiana tabacum L.and stable expression in transgenic Arabidopsis thaliana showed that the DsCBL4 protein was localized to the cell membrane.These results suggested that DsCBL4 might be involved in the abiotic stress response of Dianthus spiculifolius through the calcium signaling pathway,providing a theoretical basis for understanding its molecular mechanism.This study provided an important reference for further exploring the role of the DsCBLs gene family in plant stress resistance.
文摘Neurodegenerative disorders,including Alzheimer’s disease(AD),Parkinson’s disease(PD),and amyotrophic lateral sclerosis,impose a considerable social and economic burden on society and have dramatic consequences for individuals and their families.The majority of existing interventions have been found to be capable of only a slight modification of disease progression or to moderately delay significant functional decline in motor,cognitive,or mental domains.
基金supported by the Yonsei University Research Fund of 2021(2021-22-0060).
文摘Clustering analysis identifying unknown heterogenous subgroups of a population(or a sample)has become increasingly popular along with the popularity of machine learning techniques.Although there are many software packages running clustering analysis,there is a lack of packages conducting clustering analysis within a structural equation modeling framework.The package,gscaLCA which is implemented in the R statistical computing environment,was developed for conducting clustering analysis and has been extended to a latent variable modeling.More specifically,by applying both fuzzy clustering(FC)algorithm and generalized structured component analysis(GSCA),the package gscaLCA computes membership prevalence and item response probabilities as posterior probabilities,which is applicable in mixture modeling such as latent class analysis in statistics.As a hybrid model between data clustering in classifications and model-based mixture modeling approach,fuzzy clusterwise GSCA,denoted as gscaLCA,encompasses many advantages from both methods:(1)soft partitioning from FC and(2)efficiency in estimating model parameters with bootstrap method via resolution of global optimization problem from GSCA.The main function,gscaLCA,works for both binary and ordered categorical variables.In addition,gscaLCA can be used for latent class regression as well.Visualization of profiles of latent classes based on the posterior probabilities is also available in the package gscaLCA.This paper contributes to providing a methodological tool,gscaLCA that applied researchers such as social scientists and medical researchers can apply clustering analysis in their research.
基金supported by National Natural Science Foundation of China(Grant No.:32301161)the Natural Scientific Foundation of Hunan Province,China(Grant No.:2023JJ60052)+3 种基金the Scientific Research Project of Hunan Provincial Health Commission,China(Grant No.:202112062218,20190161)the Scientific Research Project of Hunan Provincial Department of Education,China(Grant No.:22B0455)the Clinical“4310”Project of the University of South China,China(Grant No.:20224310NHYCG02)the Doctoral Scientific Research Foundation of University of South China,China(Grant No.:200XQD042).
文摘Infrared(IR)spectroscopy,a technique within the realm of molecular vibrational spectroscopy,furnishes distinctive chemical signatures pivotal for both structural analysis and compound identification.A notable challenge emerges from the misalignment between the mid-IR light wavelength range and molecular dimensions,culminating in a constrained absorption cross-section and diminished vibrational absorption coefficients(Supplementary data).
基金National Natural Science Foundation of China(No.52375236)Fundamental Research Funds for the Central Universities,China(No.23D110316)。
文摘In the structural reliability analysis,the first-order reliability method(FORM)often yields significant errors when addressing nonlinear problems.Although the second-order reliability method(SORM)can provide higher accuracy,the additional computation of the Hessian matrix leads to lower computational efficiency.Additionally,when the dimensionality of the random variables is high,the approximation formula of SORM can result in larger errors.To address these issues,a structural reliability analysis method based on Kriging and spherical cap area integral is proposed.Firstly,this method integrates FORM with the quasi-Newton algorithm Broyden-Fletcher-Goldfarb-Shanno(BFGS),trains the Kriging model by using sample points from the algorithm’s iteration process,and combines the Kriging model with gradient information to approximate the Hessian matrix.Then,the failure surface is approximated as a rotating paraboloid,utilizing the spherical cap to replace the complex surface.For the n-dimensional case,the hyperspherical cap area expression is combined with the integral method to calculate the failure probability.Finally,the method is validated through three examples,demonstrating improved computational accuracy and efficiency compared to traditional methods.
基金Supported by Langfang Teachers College Research Grant(LSZB200803)~~
文摘[Objective] The study was to analyze the structure and function of HpaGXoo and the relationship between the two.[Method] Some related bioinformatics analysis software on internet such as NPSA,Swiss-Model,SAPS and InterPro Scan were adopted to analyze the structure and predict its function.[Result] HpaGXoo consists of 139 amino acids,and has many alpha-helical and coiled structure,no signal peptide on N-terminal and no transmembrane structure.It locates in bacterial cytoplasm.[Conclusion] The study will lay ...
文摘A detailed understanding of seismicity originating from the Nanga Parbat syntaxis in the northwestern Himalaya is crucial for characterizing the active fault systems and associated neotectonic processes in the region.Continuous earthquake monitoring through local seismic stations enables high-precision results by constraining the velocity structure.In this study,seismogram data from 244 small-magnitude earthquakes are analyzed to delineate the crustal thickness and investigate the source mechanisms beneath the Nanga Parbat syntaxis.The results are achieved with the application of Coupled Hypocenter Velocity Inversion(CHVI)analysis and Time Domain Moment Tensor(TDMT)analysis.The velocity inversion suggests that the Moho discontinuity lies at 60 km depth with an average vP/vS ratio of 1.735±0.017.The minimum 1D velocity model obtained through velocity inversion with least RMS error is further utilized in determining the source mechanism solution.In contrast to earlier studies,which highlighted strike-slip displacement accompanied by reverse dip-slip components,the present research provides a revised interpretation.The moment tensor analysis conducted in this study provides evidence of transtensional deformation associated with neotectonics,attributed to the presence of multiple shear zones.The results of the source mechanism for the selected earthquakes unveiled that the oblique-slip deformation is significantly controlled by the shear stresses coupled with the normal component of dip-slip movement.This is further supported by the higher values of the doublecouple moment tensor(85%),which indicate shear deformation,while the positive value of the compensated linear vector dipole(15%)confirms the presence of a normal component.The coexistence of transpressive and transtensive stresses,together with shallow hypocentral depths and high-amplitude tangential waveforms,can potentially cause devastating impacts in the surroundings of the Nanga Parbat syntaxis.
基金supported by the National Natural Science Foundation of China (52402298, 52172224, 52202228, 22479112)the Science and Technology Correspondent Project of Tianjin(24YDTPJC00240)+3 种基金Science Research Project of Hebei Education Department (BJK2022011)Central Funds Guiding the Local Science and Technology Development of Hebei Province (236Z4404G)the Beijing Tianjin Hebei Basic Research Cooperation Special Project(E2024202273)Tianjin Sci.&Tech. Program (22YFYSHZ00220)
文摘O3-types layered cathode materials in sodium-ion batteries(SIBs)suffer from the obvious lattice distortion induced by the complex phase transitions during Na^(+)intercalation/deintercalation process,leading to severe structural collapse and performance degradation.Herein,a series of high valence tantalum(Ta^(5+))doped Na(Ni_(0.4)Fe_(0.2)Mn_(0.4))_(1−x)Ta_(x)O_(2)(x=0/0.0025/0.005/0.01)secondary spherical particles are firstly developed,where Ta^(5+)doping enables the refined primary grain with a tightly stacked rod-like morphology.Comprehensive structural analysis via Neutron powder diffraction(NPD)and Synchrotron radiation X-ray diffraction(SXRD)reveals an expanded NaO_(2)slab and a reduction in Na site vacancy.The potential charge compensation mechanism is further illustrated by X-ray absorption spectroscopy(XAS)and X-ray photoelectron spectroscopy(XPS),unveiling a partial reduction from Ni^(3+)to Ni^(2+)with Ta^(5+)doping.In situ X-ray diffraction(in situ XRD)suggests that the decorated sample undergoes a volume change as low as 0.8%,in contrast with the pristine one(1.5%).Thus,the optimized sample with x=0.005 retains an enhanced capacity retention up to 70.4%at 1 C after 300 cycles in half-cell and delivers a high energy density of 251 Wh kg^(-1)(0.1 C)and with a good capacity retention of 81.0%at 1 C after 200 cycles in full-cell.Our findings provide new insights into the mechanism of high valence Ta^(5+)doping in stabilizing layered oxides cathode materials for SIBs.
基金Supported by the Science and Technique Bureau of Wenzhou City(No. S2005A003).
文摘Water-soluble crude polyseccharide（PIP） was extracted from cultured mycelium of the fungus Phellinus igniarius. After ethanol precipitation and sepharose CL-6B gel filtration, the fraction of PIP1 was obtained, which was shown to be a homogeneous polysaccharide by means of high-performance liquid chromatography. The structure of PIPt was determined by using several methods. C.,C analysis indicates that PIP1 is composed of the monosaccharides of glucose, galactose, and mannose. Their malar ratio is 3. 70： 4. 06： 1.00. The molar weight was estimated to be 17 kd via HPLC. IR, GC, partial hydrolysis with acid, pefiedate oxidation, Smith degradation, methylation, and GC-MS analysis were used for the structural analyses of PIP1. The results show that PIP1 has a small quantity of branch structure, The main glycosidic linkage of PIP1 has a β-configurafion. The main chain is made up of a large mass of glucose （ 1→3 ） and few mannose （ 1→4 ） ; the side chain is composed of glucose （ 1 →3 ） and galactose （ 1→6 ） ; the nonreduced end is composed of galactose and glucose. The side chains are branched at 6-0 of glucose（ 1→3,6） and mannose（1→4,6）. On an average, there are three branches among 20 residues. It is presumable that the existence of 1,3-linked Glc in the main and side chains is the main reason for its higher antitumor activity.
基金National Natural Science Foundation of China under Grant No.51078032the Visiting Scholar Foundation of China Scholarship Councilthe Center for Infrastructure Engineering Studies at Missouri University of Science and Technology
文摘An optimization method for the consistent evaluation of two Rayleigh damping coefficients is proposed. By minimizing an objective function such as an error term of the peak displacement of a structure, the two coefficients can be determined with response spectral analysis. The optimization method degenerates into the conventional method used in current practices when only two modes of vibration are included in the objective function. Therefore, the proposed method with all significant modes included for simplicity in practical applications results in suboptimal damping coefficients. The effects of both spatial distribution and frequency content of excitations as well as structural dynamic characteristics on the evaluation of Rayleigh damping coefficients were investigated with a five-story building structure. Two application examples with a 62-story high-rise building and a 840 m long cable-stayed bridge under ten earthquake excitations demonstrated the accuracy and effectiveness of the proposed method to account for all of the above effects.
基金This project is supported by National Natural Science Foundation of China(No.10472035).
文摘Conventional element based methods for modeling acoustic problems are limited to low-frequency applications due to the huge computational efforts.For high-frequency applications,probabilistic techniques,such as statistical energy analysis(SEA),are used.For mid-frequency range,currently no adequate and mature simulation methods exist.Recently,wave based method has been developed which is based on the indirect TREFFTZ approach and has shown to be able to tackle problems in the mid-frequency range.In contrast with the element based methods,no discretization is required.A sufficient,but not necessary,condition for convergence of this method is that the acoustic problem domain is convex.Non-convex domains have to be partitioned into a number of(convex)subdomains.At the interfaces between subdomains,specific coupling conditions have to be imposed.The considered two-dimensional coupled vibro-acoustic problem illustrates the beneficial convergence rate of the proposed wave based prediction technique with high accuracy.The results show the new technique can be applied up to much higher frequencies.
基金supported by National Natural Science Foundation of China(Grant No.10772060)Heilongjiang Provincial Natural Science Foundation with Excellent Young Investigators of China(GrantNo.JC2006-13)
文摘Spot weld models are widely used in finite element analysis(FEA)of automotive body in white(BIW)to predict static,dynamic,durability and other characteristics of automotive BIW.However,few researches are done on evaluation of the validity of these spot weld models in structural dynamic analysis of BIW.To evaluate the validity and accuracy of spot weld models in structural dynamic analysis of BIW,two object functions,error function and deviation function,are introduced innovatively.Modal analysis of Two-panel and Double-hat structures,which are the dominated structures in BIW,is conducted,and the values of these two object functions are obtained.Based on the values of object functions,the validity of these spot weld models are evaluated.It is found that the area contact method(ACM2)and weld element connection(CWELD)can give more precise prediction in modal analysis of these two classical structures,thus are more applicable to structural dynamic analysis of automotive BIW.Modal analysis of a classical BIW is performed,which further confirms this evaluation.The error function and deviation function proposed in this research can give guidance on the adaptability of spot weld models in structural dynamic analysis of BIW.And this evaluation method can also be adopted in evaluation of other finite element models in static,dynamic and other kinds of analysis for automotive structures.
基金the National Natural Science Foundation of China(21975154)the Shanghai Municipal Education Commission(Innovation Program(2019-01-07-00-09E00021)+2 种基金Innovative Research Team of High-level Local Universities in Shanghaisupported by The Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher LearningShanghai Key Laboratory of Materials Protection and Advanced Materials in Electric Power。
文摘With great superiorities in energy density,rate capability and structural stability,Na_(3)V_(2)(PO_(4))_(2) F_(3)(NVPF)has attracted much attentions as cathode of sodium ion battery(SIB),but it also faces challenges on its poor intrinsic electronic conductivity and the controversial de/sodiation mechanism.Herein,a series of Zr-doped NVPF coated by N-doped carbon layer(~5 nm in thickness,homogenously)materials are fabricated by a sol-gel method,and the optimized heteroatom-doping amounts of Zr and N doping improve intrinsic properties on enlarging lattice distance and enhancing electronic conductivity,respectively.Specifically,among all samples of Na_(3) V_(2-x)Zr_(x)(PO_(4))_(2) F_(3)/NC(NVPF-Zr-x/NC,x=0,0.01,0.02,0.05,and 0.1),the optimized electrode of NVPF-Zr-0.02/NC delivers high reversible capacities(119.2 mAh g^(-1) at0.5 C),superior rate capability(98.1 mA h g^(-1) at 20 C)and excellent cycling performance.The structural evolution of NVPF-Zr-0.02/NC electrode,in-situ monitored by X-ray diffractometer,follows a step-wise Na-extraction/intercalation mechanism with reversible multi-phase changes,not just a solid-solutionreaction one.Full cells of NVPF-Zr-0.02/NC//hard carbon demonstrate high capacity(99.8 mA h g^(-1) at 0.5 C),high out-put voltage(3.5 V)and good cycling stability.This work is favorable to accelerate the development of high-performance cathode materials and explore possible redox reaction mechanisms of SIBs.
基金financially supported by the China Geological Survey(Grant Nos.DD20230229,DD20160083,DD20190011,DD20221643-5)the National Key Research and Development Program of China(the DREAM—Deep Resource Exploration and Advanced Mining+1 种基金Grant No.2018YFC0603701)the Cooperative Project between the Chinese Academy of Geological Sciences and the Sinopec Shengli Oilfield Company(Grant No.P22065)。
文摘The North Qilian Shan fold and thrust belt,located at the northern Tibetan Plateau and southern margin of the Hexi Corridor,is a key tectonic unit to decode the formation and expansion of the plateau.Previous studies emphasize the Cenozoic deformation due to the far-field response to the Indo-Asian collision,but the Mesozoic deformations are poorly constrained in this area.We conducted detailed field mapping,structural analysis,geochronology,and structural interpretation of deep seismic reflectional profiling and magnetotelluric(MT)sounding,to address the superposed results of the Mesozoic and Cenozoic deformation.The results recognized the North Qilian thrust and nappe system(NQTS),the root and the frontal belt are the North Qilian thrust(NQT),and the Yumu Shan klippe(YK),respectively.The middle belt is located between the NQT and the YK.Monzonitic granite zircon U-Pb dating from the middle belt yields an age of ca.415 Ma,which is similar to south NQT.The thrusting displacement is estimated at ca.48 km by structural interpretation of deep profiles.The timing is constrained in the early stage of the Early Cretaceous by the formation of simultaneous growth strata.We suggest that the NQTS has resulted from the far-field effect of the Lhasa-Qiangtang collision,and the Yumu Shan is uplifted by the superposed Cenozoic deformation.
基金funded by the National Key Research and Development Plan(Grant No.2017YFD0101805)the National Science and Technology Foundation(Grant No.31501761)+2 种基金the National Modern Agricultural Industry Technology System Construction Special Fund Project(Grant No.CARS-23-A8)the Chinese Academy of Agricultural Sciences Science and Technology Innovation Project(Grant No.CAAS-ASTIP-IVF-CAAS)the State Key Laboratory of Vegetable Germplasm Innovation.
文摘To better understand the genetic diversity and population structure of broccoli cultivars planted in China,a total of 161 representative broccoli cultivars in the past 25 years were collected and analysed based on single nucleotide polymorphism(SNP)markers.Ten pairs of primers with good polymorphism and high resolution were screened from 315 pairs of SNP primers by 3 broccoli accessions(inbred lines)with different phenotypes and maturity.The 10 pairs of SNP primers were selected,producing 78 alleles.The diversity analysis indicated that the polymorphism information content(PIC)of SNP primer ranged from 0.64 to 0.90.The observed number of alleles(Na)was 2.00,the effective number of alleles(Ne)was 1.11–2.00,the Nei’s gene diversity(H)was 0.10–0.50,and Shannon information index(I)was 0.20–0.70 using PopGene32 software.The clustering results showed that the 161 broccoli cultivars could be divided into 4 major subgroups(A,B,C and D),foreign cultivars were all assigned to subgroup A,and domestic cultivars were assigned to 3 subgroups of B,C,and D.This study indicated that some domestic cultivars and foreign cultivars were similar in genetic background,but most domestic cultivars were still different from the Japanese cultivars.When K=2,the population structure result presented that 161 broccoli cultivars could be divided into 1 simple group(2 groups)and 1 mixed group.When Q≥0.6,143(88.82%)broccoli cultivars belonged to the simple groups.In simple groups 68(42.24%)broccoli cultivars of group 1 were derived from Japan,the United States,Switzerland,the Netherlands,China-Taiwan,and China-Mainland;75(46.58%)broccoli cultivars belonged to group 2;when Q<0.6,18(11.18%)broccoli cultivars belonged to the mixed groups.This study is helpful to understand the diversity and resolution of broccoli cultivars from worldwide,which is beneficial to plant breeding and materials innovation.And meanwhile,this result is also used for construction of broccoli fingerprint serving for cultivar identification.
文摘According to the hydraulic calculation principles of the orifice outflow, the discharge capacity of the columnar reversing gate under the partial opening condition was calculated and checked. Using ANSYS, a large finite element analysis software, the discharge process was simulated. The distribution rule of the velocities in the gate chamber and downstream channel was obtained. An FEM model of the columnar reversing gate was built, and the natural vibration properties of the gate were analyzed. Based on the Westergaard added mass method, the added mass caused by the fluid-structure coupling motion was taken into account, and the effects of the coupling interaction were discussed. The results show that the size of the small gates meets the demand for discharge capacity, the current in the gate chamber is quite turbulent, the trunnion and arms are obviously impacted by flow, and the effects of water on vibration characteristics are remarkable. The study provides a reference for the design and calculation of gates of the same type.
基金supported by National Excellent Teacher's Plan,Ministry of Education,China and Natural Science Foundation of Hebei Province,China(302318).
文摘Thirteen fractions from the pathogenic plant fungus Setosphaeria turcica race 1 were separated and collected using high performance liquid chromatography （HPLC）. Their toxic activities were assayed through leaf puncturing on corn differentials （OH43, OH43Ht1, OH43Ht2, and OH43HtN）, and the results revealed that eight fractions were toxic and fraction 6 was specifically toxic to OH43Ht1, which could be taken as a gene-selective toxin fraction. Fraction 6 was finely purified via HPLC and condensed by freeze desiccation. Its chemical structure was analyzed with EI-MS, IR, HMBC, ^1H-NMR, and two-dimensional NMR. The results suggested that fraction 6 contained an unsaturated double bond, carbonyl and methylene groups with molecular weight of 142.
基金supported by the Major National S & T Program (No. 2008ZX050009-001-01)
文摘Compressional region usually forms complex thrust faults system, which is difficult to identify using traditional migration profiles. The successful application of three-dimensional（3D） seismic attributes analysis greatly reduces the difficulty, and improves the accuracy and efficiency of seismic interpretation and structural analysis. In this paper, we took Qiongxi area in the compressional region of western Sichuan as an example, using two 3D seismic attributes, coherence and instantaneous phase, to identify fault assemblages and variations both vertically and laterally. The results show that the study area mainly consists of NS-, NE- and NEE-trending faults. The NS-trending faults are the largest and have a component of sinistral slip controlling the formation of NEE-trending faults, while the NE-trending faults are intermediate in scale, formed earlier and were cut by the NS-trending faults. Our results demonstrate that using seismic attributes for structural analysis have the following advantages：（1） more details of major fault zones,（2） highlighting minor faults which are hardly traced in seismic migration cube, and（3） easier acquisition of accurate fault systems. The application of seismic attributes provides a new idea for deciphering fine and complicated structures, and will significantly contribute to the development of objective and precise geological interpretation in the future.
文摘In the tubular frames with simple joints, the joints may show considerable local flexibility, which causes excessive deformation and affects internal force distributions. It is useful and important to study turbular joint flexibility as well as the effect of joint local flexibility on the global behavior of tubular structure for the design and building of offshore platforms. A new method for computing the stiffness coefficients of simple tubular joints is developed in this paper based on two previous computing models. According to this method, the local flexibility of T, Y, TY tubular joints are computed. This method has merits of clear physical sense, simplicity of computations and less c.p.u. time. The effect of flexibility of tubular joints on the global behavior of framed tubular structures is investigated by comparing the computed results of tubular structures with flexible tubular joints with those with rigid connections. The comparison shows that the consideration of local flexibility of tubular joints and its effect on the stress analysis of offshore structures has its practical significance.
