Biofilms have important effects on nutrient cycling in aquatic ecosystems.However,publications about the community structure and functions under laboratory conditions are rare.This study focused on the developmental a...Biofilms have important effects on nutrient cycling in aquatic ecosystems.However,publications about the community structure and functions under laboratory conditions are rare.This study focused on the developmental and physiological properties of cultured biofilms under various phosphorus concentrations performed in a closely controlled continuous flow incubator.The results showed that the biomass（Chl a）and photosynthesis of algae were inhibited under P-limitation conditions,while the phosphatase activity and P assimilation rate were promoted.The algal community structure of biofilms was more likely related to the colonization stage than with the phosphorus availability.Cyanobacteria were more competitive than other algae in biofilms,particularly when cultured under low P levels.A dominance shift occurred from non-filamentous algae in the early stage to filamentous algae in the mid and late stages under P concentrations of 0.01,0.1 and 0.6 mg/L.However,the total N content,dry weight biomass and bacterial community structure of biofilms were unaffected by phosphorus availability.This may be attributed to the low respiration rate,high accumulation of extracellular polymeric substances and high alkaline phosphatase activity in biofilms when phosphorus availability was low.The bacterial community structure differed over time,while there was little difference between the four treatments,which indicated that it was mainly affected by the colonization stage of the biofilms rather than the phosphorus availability.Altogether,these results suggested that the development of biofilms was influenced by the phosphorus availability and/or the colonization stage and hence determined the role that biofilms play in the overlying water.展开更多
Background： To our knowledge, there is little study on the interaction between nutrient availability and molecular structure changes induced by different processing methods in dairy cattle. The objective of this stud...Background： To our knowledge, there is little study on the interaction between nutrient availability and molecular structure changes induced by different processing methods in dairy cattle. The objective of this study was to investigate the effect of heat processing methods on interaction between nutrient availability and molecular structure in terms of functional groups that are related to protein and starch inherent structure of oat grains with two continued years and three replication of each year.Method： The oat grains were kept as raw（control） or heated in an air-draft oven（dry roasting： DO） at 120 °C for 60 min and under microwave irradiation（MIO） for 6 min. The molecular structure features were revealed by vibrational infrared molecular spectroscopy.Results： The results showed that rumen degradability of dry matter, protein and starch was significantly lower（P 〈0.05） for MIO compared to control and DO treatments. A higher protein α-helix to β-sheet and a lower amide I to starch area ratio were observed for MIO compared to DO and/or raw treatment. A negative correlation（-0.99, P 〈 0.01）was observed between α-helix or amide I to starch area ratio and dry matter. A positive correlation（0.99, P 〈 0.01） was found between protein β-sheet and crude protein.Conclusion： The results reveal that oat grains are more sensitive to microwave irradiation than dry heating in terms of protein and starch molecular profile and nutrient availability in ruminants.展开更多
Shelf-basin advection is essential to subsistence of the Arctic copepod Calanus hyperboreus population in high basin area. Its abundance, population structure and body size in pelagic layer were investigated with samp...Shelf-basin advection is essential to subsistence of the Arctic copepod Calanus hyperboreus population in high basin area. Its abundance, population structure and body size in pelagic layer were investigated with samples collected over a large range in the western Arctic Ocean during summer 2003, to evaluate the geographical variation in recruitment pattern. Calanus hyperboreus was absent from the shallow areas of the Chukchi Sea and most abundant in the slope area between the Chukchi Sea and Chukchi Abyssal Plain（CS-slope）. Total abundance varied between 1 110.0 and 5 815.0 ind./m^2 in the CS-slope area and ranged from 40.0 to 950.0 ind./m^2 in the other areas. Early stages（CI–IV） dominated in the CS-slope area, whereas CV and adult females were frequently recorded only in deep basin areas. Geographical difference of prosome length was most evident in CIII,with average ranging from 2.48 to 2.61 mm at the CS-slope stations and 2.16–2.37 mm at the others. Abundance of early developmental stages（CI–CIV） correlated positively with Chl a concentration, but negative correlation was observed in late stages（CV–adult）. Our results indicated that C. hyperboreus can benefit from primary production increase through accelerated development in the first growth season and the productive CS-slope area is a potential source for slope-basin replenishment.展开更多
Preventing urban regions from seismic wave destruction is of paramount significance because it is closely related to urban lifeline and prosperity of cities.Almost all conventional seismic resistance approaches rely o...Preventing urban regions from seismic wave destruction is of paramount significance because it is closely related to urban lifeline and prosperity of cities.Almost all conventional seismic resistance approaches rely on the stiffness and strength of buildings,which require excessive structural components with additional self-weights.In this study,we propose a pine-like seismic metamaterial for efficient attenuation of surface Rayleigh waves.The pine arrays in suburban regions demonstrate an ability to convert Rayleigh waves to shear bulk waves or attenuate waves on the surface via local resonation.This property originates from a gradient design of pine arrays,where a scale ratio is defined to tune the geometric properties of each pine unit.Specifically,a gradient pine array with a scale ratio smaller than one can interact with the Rayleigh waves and convert them to shear waves that propagate deep to underground.The transmission ratio of the entire system indicates a broadband wave attenuation at subwavelength scale.It reveals that the pine is able to couple with a certain elastic Rayleigh wave whose wavelength is much larger than the lattice constant,which is rarely achieved in any conventional civil engineering structures such as open trench barriers and filled trench barriers.Additionally,a numerical model of an urban region and suburban pine array is established and analyzed.Infrastructures and structures in a city that suffer direct Rayleigh wave interference run into a high risk of structural destruction as compared to urban structures protected with suburban metamaterial pine arrays.Finally,two real earthquake wave signals are used to validate the efficiency of the pine arrays in dissipating earthquake energy.The approach in this paper can be extended to deal with more complex naturally available structures for examining the elastic wave attenuation abilities of these novel structures.展开更多
基金supported by the Major Science and Technology Program for Water Pollution Control and Treatment(No2012ZX07103003-02)
文摘Biofilms have important effects on nutrient cycling in aquatic ecosystems.However,publications about the community structure and functions under laboratory conditions are rare.This study focused on the developmental and physiological properties of cultured biofilms under various phosphorus concentrations performed in a closely controlled continuous flow incubator.The results showed that the biomass（Chl a）and photosynthesis of algae were inhibited under P-limitation conditions,while the phosphatase activity and P assimilation rate were promoted.The algal community structure of biofilms was more likely related to the colonization stage than with the phosphorus availability.Cyanobacteria were more competitive than other algae in biofilms,particularly when cultured under low P levels.A dominance shift occurred from non-filamentous algae in the early stage to filamentous algae in the mid and late stages under P concentrations of 0.01,0.1 and 0.6 mg/L.However,the total N content,dry weight biomass and bacterial community structure of biofilms were unaffected by phosphorus availability.This may be attributed to the low respiration rate,high accumulation of extracellular polymeric substances and high alkaline phosphatase activity in biofilms when phosphorus availability was low.The bacterial community structure differed over time,while there was little difference between the four treatments,which indicated that it was mainly affected by the colonization stage of the biofilms rather than the phosphorus availability.Altogether,these results suggested that the development of biofilms was influenced by the phosphorus availability and/or the colonization stage and hence determined the role that biofilms play in the overlying water.
基金supported by grants from the Prairie Oat Grower Association(POGA)Natural Sciences and Engineering Research Council of Canada(NSERC-federal government)Ministry of Agriculture Strategic Research Chair(PY)Program
文摘Background： To our knowledge, there is little study on the interaction between nutrient availability and molecular structure changes induced by different processing methods in dairy cattle. The objective of this study was to investigate the effect of heat processing methods on interaction between nutrient availability and molecular structure in terms of functional groups that are related to protein and starch inherent structure of oat grains with two continued years and three replication of each year.Method： The oat grains were kept as raw（control） or heated in an air-draft oven（dry roasting： DO） at 120 °C for 60 min and under microwave irradiation（MIO） for 6 min. The molecular structure features were revealed by vibrational infrared molecular spectroscopy.Results： The results showed that rumen degradability of dry matter, protein and starch was significantly lower（P 〈0.05） for MIO compared to control and DO treatments. A higher protein α-helix to β-sheet and a lower amide I to starch area ratio were observed for MIO compared to DO and/or raw treatment. A negative correlation（-0.99, P 〈 0.01）was observed between α-helix or amide I to starch area ratio and dry matter. A positive correlation（0.99, P 〈 0.01） was found between protein β-sheet and crude protein.Conclusion： The results reveal that oat grains are more sensitive to microwave irradiation than dry heating in terms of protein and starch molecular profile and nutrient availability in ruminants.
基金The National Natural Science Foundation of China under contract No.40406002the Public Science and Technology Research Funds Projects of Ocean under contract No.201105022
文摘Shelf-basin advection is essential to subsistence of the Arctic copepod Calanus hyperboreus population in high basin area. Its abundance, population structure and body size in pelagic layer were investigated with samples collected over a large range in the western Arctic Ocean during summer 2003, to evaluate the geographical variation in recruitment pattern. Calanus hyperboreus was absent from the shallow areas of the Chukchi Sea and most abundant in the slope area between the Chukchi Sea and Chukchi Abyssal Plain（CS-slope）. Total abundance varied between 1 110.0 and 5 815.0 ind./m^2 in the CS-slope area and ranged from 40.0 to 950.0 ind./m^2 in the other areas. Early stages（CI–IV） dominated in the CS-slope area, whereas CV and adult females were frequently recorded only in deep basin areas. Geographical difference of prosome length was most evident in CIII,with average ranging from 2.48 to 2.61 mm at the CS-slope stations and 2.16–2.37 mm at the others. Abundance of early developmental stages（CI–CIV） correlated positively with Chl a concentration, but negative correlation was observed in late stages（CV–adult）. Our results indicated that C. hyperboreus can benefit from primary production increase through accelerated development in the first growth season and the productive CS-slope area is a potential source for slope-basin replenishment.
基金supported by City University of Hong Kong(Project No.ARG 9667253)Fundamental Research Funds for the Central Universities(Grant Number RF1028623346).
文摘Preventing urban regions from seismic wave destruction is of paramount significance because it is closely related to urban lifeline and prosperity of cities.Almost all conventional seismic resistance approaches rely on the stiffness and strength of buildings,which require excessive structural components with additional self-weights.In this study,we propose a pine-like seismic metamaterial for efficient attenuation of surface Rayleigh waves.The pine arrays in suburban regions demonstrate an ability to convert Rayleigh waves to shear bulk waves or attenuate waves on the surface via local resonation.This property originates from a gradient design of pine arrays,where a scale ratio is defined to tune the geometric properties of each pine unit.Specifically,a gradient pine array with a scale ratio smaller than one can interact with the Rayleigh waves and convert them to shear waves that propagate deep to underground.The transmission ratio of the entire system indicates a broadband wave attenuation at subwavelength scale.It reveals that the pine is able to couple with a certain elastic Rayleigh wave whose wavelength is much larger than the lattice constant,which is rarely achieved in any conventional civil engineering structures such as open trench barriers and filled trench barriers.Additionally,a numerical model of an urban region and suburban pine array is established and analyzed.Infrastructures and structures in a city that suffer direct Rayleigh wave interference run into a high risk of structural destruction as compared to urban structures protected with suburban metamaterial pine arrays.Finally,two real earthquake wave signals are used to validate the efficiency of the pine arrays in dissipating earthquake energy.The approach in this paper can be extended to deal with more complex naturally available structures for examining the elastic wave attenuation abilities of these novel structures.
