The viscosity of fracturing fluid and in-situ stress difference are the two important factors that affect the hydraulic fracturing pressure and propagation morphology. In this study, raw coal was used to prepare coal ...The viscosity of fracturing fluid and in-situ stress difference are the two important factors that affect the hydraulic fracturing pressure and propagation morphology. In this study, raw coal was used to prepare coal samples for experiments, and clean fracturing fluid samples were prepared using CTAB surfactant. A series of hydraulic fracturing tests were conducted with an in-house developed triaxial hydraulic fracturing simulator and the fracturing process was monitored with an acoustic emission instrument to analyze the influences of fracturing fluid viscosity and horizontal in-situ stress difference on coal fracture propagation. The results show that the number of branched fractures decreased, the fracture pattern became simpler, the fractures width increased obviously, and the distribution of AE event points was concentrated with the increase of the fracturing fluid viscosity or the horizontal in-situ stress difference. The acoustic emission energy decreases with the increase of fracturing fluid viscosity and increases with the increase of horizontal in situ stress difference. The low viscosity clean fracturing fluid has strong elasticity and is easy to be compressed into the tip of fractures, resulting in complex fractures. The high viscosity clean fracturing fluids are the opposite. Our experimental results provide a reference and scientific basis for the design and optimization of field hydraulic fracturing parameters.展开更多
BACKGROUND： Prenatal stress has been shown to inhibit cell proliferation in the dentate gyrus and hippocampus, reduce hippocampal volume, and cause neuronal loss and oxidative damage in the hippocampus of offspring r...BACKGROUND： Prenatal stress has been shown to inhibit cell proliferation in the dentate gyrus and hippocampus, reduce hippocampal volume, and cause neuronal loss and oxidative damage in the hippocampus of offspring rats, but the sexual difference of the effects on offsprings is seldom referred to. OBJECTIVE： To observe the effect of prenatal stress to adult pregnant rats on expression of extracellular signal-regulated kinases （ERK） in hippocampus of the offspring rats of different genders. DESIGN ： A randomized and control animal experiment.SETTING： Department of Physiology and Pathophysiology, School of Medicine, Xi＇an Jiaotong University. MATERIALS ： The experiments were carried out in the Key Laboratory of Environment and Gene Related Diseases （Xi＇an Jiaotong University）, Ministry of Education between October 2005 and March 2006. Fifteen female and five male adult Sprague-Dawley rats were adopted. Female rats weighing 230-250 g and male rats weighing 280-350 g were used. METHODS： The virgin female rats were placed overnight with adult male rats （3：1） for mating. A total of twelve pregnant rats were randomly assigned to prenatal stress group （PNS group, n=6） and control group （n=6）. The pregnant rats of the PNS group were exposed to restraint stress on days 14-20 of pregnancy three times a day, 45 minutes for each time . The restraint device was a transparent plastic tube （6.8 cm in diameter） with air holes for breathing and closed end. The length could be adjusted to accommodate the size of the animals. To prevent habituation of animals to the daily procedure, restraint periods were randomly shifted within certain time periods （8：00-11：00, 11：00-14：00, and 16：00-19：00）. After birth, offsprings of all groups were culled to 8-10 litters in each group and housed in the same animal room, and kept together with their biologic mothers. The pregnant rats of the control group were left undisturbed. On day 21, after all the offspring were weaned, male and female pups were separated and housed four in each cage respectively until test at 30 days of age. At the end of postnatal day 30, one male and female offspring rats from the same dam were selected with a random choice and a total of 24 animals from 12 different dams were used. The experimental rats were sacrificed by decapitation under anesthesia. Bilateral hippocampal tissues were isolated and homogenized in cold condition. Alkaline carbonate buffer （BCA） method was used to detect the concentration of extracellular signal-regulated kinases （ERK）, then mixed with loading buffer, the constant voltage was 100 V. Finally, BCIP/NBT staining and electrDphoresis were performed, the absorbance （A） value for the bands was detected with the Bandscan analytical software, and the expression of ERK in hippocampus of offspring rats of different genders in each group was quantitatively analyzed. MAIN OUTCOME MEASURES： The level of ERK expression in hippocampus of offspring rats of different genders in each group was observed.RESULTS： All the 24 offspring rats were involved in the analysis of results. ① The staining results of ERP activity in the extract of brain tissue detected with Western blotting technique and specific antibody analysis showed that the ERP in hippocampus of offspring rats had two subtypes of ERK-1 and ERK-2, and the latter was the main type.② Standardized by the average A value in the control group, the quantitative data of the general A value of total ERK showed that the expression of ERK-2 in hippocampus of female offspring rats was obviously higher in the PNS group than in the control group （A value： 126±6.76,100±4.89,P〈 0.01）. ③The expression of ERK-2 had no obvious difference between the female and male offspring rats in the control group.④ The expression of ERK-2 in hippocampus of male offspring rats was a little higher in the PNS group than in the control group （A value： 104±6.27,102±5.48,P 〉 0.05）. CONCLUSION ： PNS significantly affects the increase of ERK expression in hippocampus of female offspring rats, but has no obvious influence on that of male ones.展开更多
Background: Chronic stress is a emotional challenge. Constant pressure presents a serious risk of damage to mental and physical health and hence is associated with increased incidence of various diseases. The ability ...Background: Chronic stress is a emotional challenge. Constant pressure presents a serious risk of damage to mental and physical health and hence is associated with increased incidence of various diseases. The ability to cope with chronic stress may be a function of psychological resilience including intellectual capacities, but more so of external factors such as life experience and education. Adolescents are more vulnerable to chronic stress than adults. The measures introduced during the COVID-19 pandemic brought up major societal problems. As both children and adolescents lost their life anchors, the prevalence of stress in adolescents increased from 20% to 45%. Chronic psychological stress can impede the normal development of schoolchildren. It may cause anxiety, social withdrawal, interpersonal conflicts and aggression. This applies particularly to those in puberty, with the adolescent already facing unstable social bonds and elevated fear about the future. It is likely that the puberty cohort accounts for the dramatic increase in the prevalence of stress. Since it impacts public health, chronic stress among school-age children is increasingly taking on a socio-political dimension. Non-clinical stress intervention studies can investigate how to achieve stress reduction in school children. Methods: In a small pilot study, we analysed the effects of a training program with four different standard interventions, i.e. mindfulness training, progressive muscle reflection, autogenic training, and sound meditation. We obtained baseline scores of 10 stress-indicators, and re-tested after the interventions were performed. Results: The four applied interventions resulted in a reduction of 8 (out of 10) stress-indicators, such as “feeling stressed” or stress related symptoms (headaches, dizziness, sweating). This positive impact of the interventions significantly reduced “Fears about the future” (p Discussion: School children recognise the positive potential of stress reducing training or interventions. Our results provide evidence in support of integrating the training of relaxation techniques in the school curriculum. We propose that a larger study be undertaken to determine which methods would be most effective.展开更多
Developing low-permeability Coalbed Methane(CBM)reservoirs can significantly benefit from a comprehensive understanding of hydraulic fracture nucleation and propagation mechanisms,particularly in anthracite CBM reserv...Developing low-permeability Coalbed Methane(CBM)reservoirs can significantly benefit from a comprehensive understanding of hydraulic fracture nucleation and propagation mechanisms,particularly in anthracite CBM reservoirs.This study employs true-triaxial hydraulic fracturing experiments to investigate these mechanisms,with variables including injection flow rate,horizontal stress difference(σH-σh),and bedding orientation.Additionally,we conduct corresponding numerical cases to validate the experimental conclusions.The research also considers re-fracturing instances.For the first time,we utilize a combination of Kaiser tests and the stress transfer function in ANSYS Workbench finite element analysis to accurately restore the confining pressure of the coal sample.The findings suggest that a high initial injection flow rate during hydraulic fracturing can promote fluid leakage and aid in maintaining substantial fracture pressure.Enhanced fracturing efficiency can be achieved through higher injection rates,and it can ensure optimal fracturing efficiency,minimizing roof and floor fracturing in coal reservoirs to prevent fracturing fluid leakage.The presence of a high horizontal stress difference facilitates hydraulic fracture propagation along the direction of the maximum horizontal compressive stress,requiring a greater hydraulic pressure to produce more fracture systems in coal reservoirs.Additionally,a minor deviation in the wellbore injection direction from the bedding orientation assists in creating a complex hydraulic fractured network,although this also requires higher hydraulic pressure to initiate new fractures.In the case of multiple hydraulic fracturing,the second initiation pressure tends to be significantly higher than the first,indicating that a sequential increase in hydraulic pressure aids the formation of additional fractures.Moreover,a simplified numerical simulation has been conducted to corroborate the experimental findings.These insights are crucial in optimizing hydraulic fracturing processes to enhance the permeability of anthracite CBM reservoirs.展开更多
The phase volume fraction has an important role in the match of the strength and plasticity of dual phase steel.The different bainite contents(18–53 vol.%)in polygonal ferrite and bainite(PF+B)dual phase steel were o...The phase volume fraction has an important role in the match of the strength and plasticity of dual phase steel.The different bainite contents(18–53 vol.%)in polygonal ferrite and bainite(PF+B)dual phase steel were obtained by controlling the relaxation finish temperature during the rolling process.The effect of bainite volume fraction on the tensile deformability was systematically investigated via experiments and crystal plasticity finite element model(CPFEM)simulation.The experimental results showed that the steel showed optimal strain hardenability and strength–plasticity matching when the bainite reached 35%.The 3D-CPFEM models with the same grain size and texture characters were established to clarify the influence of stress/strain distribution on PF+B dual phase steel with different bainite contents.The simulation results indicated that an appropriate increase in the bainite content(18%–35%)did not affect the interphase strain difference,but increased the stress distribution in both phases,as a result of enhancing the coordinated deformability of two phases and improving the strength–plasticity matching.When the bainite content increased to 53%,the stress/strain difference between the two phases was greatly increased,and plastic damage between the two phases was caused by the reduction of the coordinated deformability.展开更多
The changes of proteins in the rice (Oryzasativa L.) Tesanai 2 seedling under salt (NaCl,4 g/L), heat shock (42℃, 3h ), and cold(14℃, 3d ) pretreatments were compared toexplore the mechanism of the cross adaptationt...The changes of proteins in the rice (Oryzasativa L.) Tesanai 2 seedling under salt (NaCl,4 g/L), heat shock (42℃, 3h ), and cold(14℃, 3d ) pretreatments were compared toexplore the mechanism of the cross adaptationto different environmental stresses. The chill-ing resistance of rice seedling after 1℃, 150pmol·msPFD(photo flux density) for 2d was enhanced distinctly by salt, heat shock,展开更多
Volumetric fracturing is a primary stimulation technology for economical and effective exploitation of tight oil reservoirs. The main mechanism is to connect natural fractures to generate a fracture network system whi...Volumetric fracturing is a primary stimulation technology for economical and effective exploitation of tight oil reservoirs. The main mechanism is to connect natural fractures to generate a fracture network system which can enhance the stimulated reservoir volume. By using the combined finite and discrete element method, a model was built to describe hydraulic fracture propagation in tight oil reservoirs. Considering the effect of horizontal stress difference, number and spacing of perforation clus- ters, injection rate, and the density of natural fractures on fracture propagation, we used this model to simulate the fracture propagation in a tight formation of a certain oil- field. Simulation results show that when the horizontal stress difference is lower than 5 MPa, it is beneficial to form a complex fracture network system. If the horizontal stress difference is higher than 6 MPa, it is easy to form a planar fracture system; with high horizontal stress differ- ence, increasing the number of perforation clusters is beneficial to open and connect more natural fractures, and to improve the complexity of fracture network and the stimulated reservoir volume （SRV）. As the injection rate increases, the effect of volumetric fracturing may be improved; the density of natural fractures may only have a great influence on the effect of volume stimulation in a low horizontal stress difference.展开更多
The main area of the Jiaoshiba anticline of the Fuling shale gas field was taken as the research object,laboratory rock mechanical experiments and direct shear experiments were conducted to clarify the mechanical anis...The main area of the Jiaoshiba anticline of the Fuling shale gas field was taken as the research object,laboratory rock mechanical experiments and direct shear experiments were conducted to clarify the mechanical anisotropy characteristics and parameters of rock samples with rich beddings.Based on the experimental results,a 3D fracture propagation model of the target reservoir taking mechanical anisotropy,weak bedding plane and vertical stress difference into account was established by the discrete element method to analyze distribution patterns of hydraulic fractures under different bedding densities,mechanical properties,and fracturing engineering parameters(including perforation clusters,injection rates and fracturing fluid viscosity).The research results show that considering the influence of the weak bedding plane and longitudinal stress difference,the interlayer stress difference 3–4 MPa in the study area can control the fracture height within the zone of stress barrier,and the fracture height is less than 40 m.If the influence of the weak bedding plane is not considered,the simulation result of fracture height is obviously higher.Although the opening of high-density bedding fractures increases the complexity of hydraulic fractures,it significantly limited the propagation of fracture height.By reducing the number of clusters,increasing the injection rate,and increasing the volume and proportion of high-viscosity fracturing fluid in the pad stage,the restriction on fracture height due to the bedding plane and vertical stress difference can be reduced,and the longitudinal propagation of fractures can be promoted.The fracture propagation model was used to simulate one stage of Well A in Fuling shale gas field,and the simulation results were consistent with the micro-seismic monitoring results.展开更多
The structure and rheological properties of carbon-based particle suspensions, i.e., carbon black（CB）, multi-wall carbon nanotube（MWNT）, graphene and hollow carbon sphere（HCS） suspended in polydimethylsiloxane（...The structure and rheological properties of carbon-based particle suspensions, i.e., carbon black（CB）, multi-wall carbon nanotube（MWNT）, graphene and hollow carbon sphere（HCS） suspended in polydimethylsiloxane（PDMS）, are investigated. In order to study the effect of particle shape on the structure and rheological properties of suspensions, the content of surface oxygen-containing functional groups of carbon-based particles is controlled to be similar. Original spherical-like CB（fractal filler）, rod-like MWNT and sheet-like graphene form large agglomerates in PDMS, while spherical HCS particles disperse relatively well in PDMS. The dispersion state of carbon-based particles affects the critical concentration of forming a rheological percolation network. Under weak shear, negative normal stress differences（ΔN） are observed in CB, MWNT and graphene suspensions, while ΔN is nearly zero for HCS suspensions. It is concluded that the vorticity alignment of CB, MWNT and graphene agglomerates under shear results in the negative ΔN. However, no obvious structural change is observed in HCS suspension under weak shear, and accordingly, the ΔN is almost zero.展开更多
Based on the research results of lab triaxial hydraulic fracturing simulation experiments,field fracturing practice,and theory analysis,the factors affecting the growth of hydraulic fracture network in shale reservoir...Based on the research results of lab triaxial hydraulic fracturing simulation experiments,field fracturing practice,and theory analysis,the factors affecting the growth of hydraulic fracture network in shale reservoirs,including brittleness,difference of horizontal stress,distribution and mechanical characteristics of natural fractures,fluid viscosity and fracturing parameters,etc are analyzed in this study.The results show that the growth of fracture network in shale reservoirs is affected by geological factors and engineering factors jointly.From the perspective of reservoir geological factors,the higher the rock brittleness,the more developed the natural fractures,and the poorer the natural fracture consolidation,the more likely hydraulic fracture network will be formed.From the perspective of fracturing engineering factors,lower fluid viscosity and larger fracturing scale will be more helpful to the formation of extensive fracture network.On the basis of the analysis of single factors,a multi-factor coupling index has been established to characterize the growth degree of hydraulic fracture network and evaluate the complexity of hydraulic fractures after the fracturing of shale reservoirs.展开更多
The Upper Ordovician Wufeng Formation and the Lower Silurian Longmaxi Formation in the central Yangtze area of southern China has a good prospect of shale gas exploration.So far,however,its complicated geological cond...The Upper Ordovician Wufeng Formation and the Lower Silurian Longmaxi Formation in the central Yangtze area of southern China has a good prospect of shale gas exploration.So far,however,its complicated geological conditions and shale gas exploration and development potentials have not been understood completely,which affects its exploration achievements.In order to determine the main factors controlling shale gas enrichment in the WufengeLongmaxi Fms in this area,this paper studied the key shale gas enrichment conditions in the black shale there(e.g.sedimentary environments,reservoir development characteristics and preservation conditions)based on single-well comprehensive reservoir evaluation,combined with drilling,logging,core,outcrop and test data.In addition,it was comparedwith the main shale gas blocks in the Sichuan Basin,such as Jiaoshiba and Changning.And the following research results are obtained.First,the organic-rich shale section in this areawas formed during the sedimentation fromWufeng Formation to the third submember of the first member of Longmaxi Formation.It is the deposit of deepwater continental facies,and its thickness is in a range of 15-39 m,presenting a trend of increasing from south to north.Second,the reservoir rocks in the high-quality shale sections are dominated by siliceous shale,and the reservoir porosity is in a range of 1.60-7.44%.The reservoir spaces are dominated by organic pores with good connectivity and high total organic carbon(TOC)content.The organic matter is of a sapropel-sapropel prone hybrid type,with a high thermal evolution degree,better gas bearing property and good fracturability.Third,the Dangyang synclinorium is characterized by better strata preservation,rock occurrence flat,less developed faults and thick and stable roofs and floors,so its shale gas preservation conditions are better.Fourth,different from the Changning and Jiaoshiba Blocks,the central Yangtze area is characterized by great burial depth,large bidirectional stress difference,and low formation pressure coefficient.In conclusion,the WufengeLongmaxi Fms is better in shale gas enrichment conditions and has a potential of further exploration.However,its commercial shale gas development and scale production increase in the future face challenges due to its large stress difference,great burial depth,and lower pressure coefficient.展开更多
To prevent dense chromium oxide covers from cracking during firing,such as internal cracking,surface cracking and fracture,the firing process of dense chromium oxide products was simulated,and the performance paramete...To prevent dense chromium oxide covers from cracking during firing,such as internal cracking,surface cracking and fracture,the firing process of dense chromium oxide products was simulated,and the performance parameters at several specific temperatures before and after densification of chromium oxide green bodies were tested.The temperature difference and the stress difference of chromium oxide green bodies were calculated by Finite Element Software at heating and cooling rates of 10,20,30 and 40℃·h^(-1),respectively.The results show that large temperature difference and thermal stress difference are the main causes of internal cracking,surface cracking and fracture of the brick.Suitable heating rates reduce or avoid internal cracking of the brick.When the heating rate is 30℃·h^(-1) below 1450℃and 10℃·h^(-1) above 1450℃,the temperature difference and the thermal stress difference in the brick are below 4℃and 4 MPa,respectively,and there is no internal crack in the brick after firing.The initial cooling stage is the key stage that causes cracking or fracture of bricks.When the cooling rate is 20℃·h^(-1) above 1450℃,the thermal stress difference in the brick is less than 4 MPa,which can significantly reduce the surface cracks and fracture of the brick.展开更多
For figuring out the stress interference of multiphase fracture combinations and its effect on the fracture initiation pressure of subsequent hydraulic fracturing,a calculation model for the coupled stress field with ...For figuring out the stress interference of multiphase fracture combinations and its effect on the fracture initiation pressure of subsequent hydraulic fracturing,a calculation model for the coupled stress field with multiple induced fractures preexisted was established based on the calculation model for the stress field with single induced fracture preexisted,and the change laws of circumferential stress field around the wellbore under the effect of induced stress were analyzed.Then,the fracture initiation pressure of subsequent hydraulic fracturing was calculated according to the fracturing mechanics criterion.Finally,the effects of the length,phase,horizontal principal stress difference co-efficient and quantity of preexisting fractures on its initiation pressure were analyzed.And the following research results were obtained.First,the circumferential stress difference in the area near the fractures behind the preexisting fractures increase greatly and even the horizontal principal stress is reversed.Second,as the length of new preexisting fractures increases,the initiation pressure of initial preexisting fractures rises first and then drops.And when the length of new preexisting fractures is equal to that of the initial preexisting fractures,the initiation pressure of initial preexisting fractures drops quickly first and then slowly.Third,the initiation pressure of high phase fractures is lower than that of low phase fractures.Fourth,with the increase of fracture quantity,the initiation pressure of new preexisting fractures drops gradually,but the decline trend of initiation pressure difference is not obvious.Fifth,the stress interference of multiphase fractures influences the fracture initiation pressure,and high-phase long fractures and low-phase short fractures are both favorable for the simultaneous initiation of multiphase fractures.Sixth,syn-chronous propagation of multiple fractures can generate more complicated stress interference and excite the evolution of hydraulic fracture network,so as to realize uniform fracturing stimulation.In conclusion,the research results can provide a theoretical guidance for the design of fracturing operation,e.g.perforation phase of blasting induced fracturing,scale of induced fractures,and pump pressure of subsequent hydraulic fracturing.展开更多
A reasonable unsupported roof distance(URD) when advancing underground coal mine roadways can contribute greatly to safe and rapid roadway development.A mechanical model of the roof,using the relationship between the ...A reasonable unsupported roof distance(URD) when advancing underground coal mine roadways can contribute greatly to safe and rapid roadway development.A mechanical model of the roof,using the relationship between the roof stress distribution and URD,obtained by the difference method,and roof stability according to the in-situ roof stress and rock mass strength was developed.We subsequently designed a proper range of URD,developed a testing method of URD with the function of mining protection,evaluated roof stability through analyzing the test data and then determined a reasonable URD.Considering the factors of the geological conditions,the immediate roof stability and the efficiency of the labor arrangement system,the URD of the advancing roadway of 9802 working face in Zhangshuanglou coal mine was determined to be 6 m using the proposed method.The results show that,when a 2 m length of roadway was reinforced by temporary support and high pre-stressed bolt support after the roadway advancement of 6 m per cycle,the speed and the security of the roadway development can be achieved and the advance rate can reach more than 400 m per month.展开更多
The main reservoir stratum of shale gas in the Jiaoshiba area, Sichuan basin is Wufeng-Longmaxi Formations. This paper calculates the tectonic difference stress values of JY1 well in different depths and numerically s...The main reservoir stratum of shale gas in the Jiaoshiba area, Sichuan basin is Wufeng-Longmaxi Formations. This paper calculates the tectonic difference stress values of JY1 well in different depths and numerically simulates the shale gas reservoir by elasto-plastic incremental method. The results of tectonic difference stress value suggest that the present tectonic stress values distribute strippedly in plane. The areas in favor of the development of fractures are stripped and mostly distributed in the northeast, middle and southwest of Jiaoshiba area, where the shale gas is concentrated.展开更多
基金National Natural Science Foundation of China (51974176, 52174194, 51934004)Shandong Provincial Colleges and Universities Youth Innovation and Technology Support Program (2019KJH006)+1 种基金Taishan Scholars Project (TS20190935)Shandong outstanding youth fund (ZR2020JQ22).
文摘The viscosity of fracturing fluid and in-situ stress difference are the two important factors that affect the hydraulic fracturing pressure and propagation morphology. In this study, raw coal was used to prepare coal samples for experiments, and clean fracturing fluid samples were prepared using CTAB surfactant. A series of hydraulic fracturing tests were conducted with an in-house developed triaxial hydraulic fracturing simulator and the fracturing process was monitored with an acoustic emission instrument to analyze the influences of fracturing fluid viscosity and horizontal in-situ stress difference on coal fracture propagation. The results show that the number of branched fractures decreased, the fracture pattern became simpler, the fractures width increased obviously, and the distribution of AE event points was concentrated with the increase of the fracturing fluid viscosity or the horizontal in-situ stress difference. The acoustic emission energy decreases with the increase of fracturing fluid viscosity and increases with the increase of horizontal in situ stress difference. The low viscosity clean fracturing fluid has strong elasticity and is easy to be compressed into the tip of fractures, resulting in complex fractures. The high viscosity clean fracturing fluids are the opposite. Our experimental results provide a reference and scientific basis for the design and optimization of field hydraulic fracturing parameters.
基金the National Natural Science Foundation of China, No. 30270445
文摘BACKGROUND： Prenatal stress has been shown to inhibit cell proliferation in the dentate gyrus and hippocampus, reduce hippocampal volume, and cause neuronal loss and oxidative damage in the hippocampus of offspring rats, but the sexual difference of the effects on offsprings is seldom referred to. OBJECTIVE： To observe the effect of prenatal stress to adult pregnant rats on expression of extracellular signal-regulated kinases （ERK） in hippocampus of the offspring rats of different genders. DESIGN ： A randomized and control animal experiment.SETTING： Department of Physiology and Pathophysiology, School of Medicine, Xi＇an Jiaotong University. MATERIALS ： The experiments were carried out in the Key Laboratory of Environment and Gene Related Diseases （Xi＇an Jiaotong University）, Ministry of Education between October 2005 and March 2006. Fifteen female and five male adult Sprague-Dawley rats were adopted. Female rats weighing 230-250 g and male rats weighing 280-350 g were used. METHODS： The virgin female rats were placed overnight with adult male rats （3：1） for mating. A total of twelve pregnant rats were randomly assigned to prenatal stress group （PNS group, n=6） and control group （n=6）. The pregnant rats of the PNS group were exposed to restraint stress on days 14-20 of pregnancy three times a day, 45 minutes for each time . The restraint device was a transparent plastic tube （6.8 cm in diameter） with air holes for breathing and closed end. The length could be adjusted to accommodate the size of the animals. To prevent habituation of animals to the daily procedure, restraint periods were randomly shifted within certain time periods （8：00-11：00, 11：00-14：00, and 16：00-19：00）. After birth, offsprings of all groups were culled to 8-10 litters in each group and housed in the same animal room, and kept together with their biologic mothers. The pregnant rats of the control group were left undisturbed. On day 21, after all the offspring were weaned, male and female pups were separated and housed four in each cage respectively until test at 30 days of age. At the end of postnatal day 30, one male and female offspring rats from the same dam were selected with a random choice and a total of 24 animals from 12 different dams were used. The experimental rats were sacrificed by decapitation under anesthesia. Bilateral hippocampal tissues were isolated and homogenized in cold condition. Alkaline carbonate buffer （BCA） method was used to detect the concentration of extracellular signal-regulated kinases （ERK）, then mixed with loading buffer, the constant voltage was 100 V. Finally, BCIP/NBT staining and electrDphoresis were performed, the absorbance （A） value for the bands was detected with the Bandscan analytical software, and the expression of ERK in hippocampus of offspring rats of different genders in each group was quantitatively analyzed. MAIN OUTCOME MEASURES： The level of ERK expression in hippocampus of offspring rats of different genders in each group was observed.RESULTS： All the 24 offspring rats were involved in the analysis of results. ① The staining results of ERP activity in the extract of brain tissue detected with Western blotting technique and specific antibody analysis showed that the ERP in hippocampus of offspring rats had two subtypes of ERK-1 and ERK-2, and the latter was the main type.② Standardized by the average A value in the control group, the quantitative data of the general A value of total ERK showed that the expression of ERK-2 in hippocampus of female offspring rats was obviously higher in the PNS group than in the control group （A value： 126±6.76,100±4.89,P〈 0.01）. ③The expression of ERK-2 had no obvious difference between the female and male offspring rats in the control group.④ The expression of ERK-2 in hippocampus of male offspring rats was a little higher in the PNS group than in the control group （A value： 104±6.27,102±5.48,P 〉 0.05）. CONCLUSION ： PNS significantly affects the increase of ERK expression in hippocampus of female offspring rats, but has no obvious influence on that of male ones.
文摘Background: Chronic stress is a emotional challenge. Constant pressure presents a serious risk of damage to mental and physical health and hence is associated with increased incidence of various diseases. The ability to cope with chronic stress may be a function of psychological resilience including intellectual capacities, but more so of external factors such as life experience and education. Adolescents are more vulnerable to chronic stress than adults. The measures introduced during the COVID-19 pandemic brought up major societal problems. As both children and adolescents lost their life anchors, the prevalence of stress in adolescents increased from 20% to 45%. Chronic psychological stress can impede the normal development of schoolchildren. It may cause anxiety, social withdrawal, interpersonal conflicts and aggression. This applies particularly to those in puberty, with the adolescent already facing unstable social bonds and elevated fear about the future. It is likely that the puberty cohort accounts for the dramatic increase in the prevalence of stress. Since it impacts public health, chronic stress among school-age children is increasingly taking on a socio-political dimension. Non-clinical stress intervention studies can investigate how to achieve stress reduction in school children. Methods: In a small pilot study, we analysed the effects of a training program with four different standard interventions, i.e. mindfulness training, progressive muscle reflection, autogenic training, and sound meditation. We obtained baseline scores of 10 stress-indicators, and re-tested after the interventions were performed. Results: The four applied interventions resulted in a reduction of 8 (out of 10) stress-indicators, such as “feeling stressed” or stress related symptoms (headaches, dizziness, sweating). This positive impact of the interventions significantly reduced “Fears about the future” (p Discussion: School children recognise the positive potential of stress reducing training or interventions. Our results provide evidence in support of integrating the training of relaxation techniques in the school curriculum. We propose that a larger study be undertaken to determine which methods would be most effective.
基金funded by the National Natural Science Foundation of China(No.42202155)China Postdoctoral Science Foundation(No.2021MD703807)+7 种基金Heilongjiang Provincial Postdoctoral Science Foundation(No.LBH-Z20121)financial support from the China Scholarship Council(No.202008230018)the Research Fund Program of Hubei Key Laboratory of Resources and Eco-Environment Geology(No.HBREGKFJJ-202309)funding by the DGICYT Spanish Project(grant no.PID2020-118999GB-I00)funded by the MCIN/AEI/10.13039/501100011033funding by the Ramón y Cajal fellowship(grant no.RyC-2018-026335-I)funded by the MCIN/AEI/10.13039/50110001103the European Social Fund-Investing in Your Future.
文摘Developing low-permeability Coalbed Methane(CBM)reservoirs can significantly benefit from a comprehensive understanding of hydraulic fracture nucleation and propagation mechanisms,particularly in anthracite CBM reservoirs.This study employs true-triaxial hydraulic fracturing experiments to investigate these mechanisms,with variables including injection flow rate,horizontal stress difference(σH-σh),and bedding orientation.Additionally,we conduct corresponding numerical cases to validate the experimental conclusions.The research also considers re-fracturing instances.For the first time,we utilize a combination of Kaiser tests and the stress transfer function in ANSYS Workbench finite element analysis to accurately restore the confining pressure of the coal sample.The findings suggest that a high initial injection flow rate during hydraulic fracturing can promote fluid leakage and aid in maintaining substantial fracture pressure.Enhanced fracturing efficiency can be achieved through higher injection rates,and it can ensure optimal fracturing efficiency,minimizing roof and floor fracturing in coal reservoirs to prevent fracturing fluid leakage.The presence of a high horizontal stress difference facilitates hydraulic fracture propagation along the direction of the maximum horizontal compressive stress,requiring a greater hydraulic pressure to produce more fracture systems in coal reservoirs.Additionally,a minor deviation in the wellbore injection direction from the bedding orientation assists in creating a complex hydraulic fractured network,although this also requires higher hydraulic pressure to initiate new fractures.In the case of multiple hydraulic fracturing,the second initiation pressure tends to be significantly higher than the first,indicating that a sequential increase in hydraulic pressure aids the formation of additional fractures.Moreover,a simplified numerical simulation has been conducted to corroborate the experimental findings.These insights are crucial in optimizing hydraulic fracturing processes to enhance the permeability of anthracite CBM reservoirs.
基金supported by the Project of Liaoning Marine Economic Development(Development of high strength pipeline steel for submarine oil and gas transmission)State Key Laboratory of Metal Material for Marine Equipment and Application Funding(No.SKLMEA-K202205).
文摘The phase volume fraction has an important role in the match of the strength and plasticity of dual phase steel.The different bainite contents(18–53 vol.%)in polygonal ferrite and bainite(PF+B)dual phase steel were obtained by controlling the relaxation finish temperature during the rolling process.The effect of bainite volume fraction on the tensile deformability was systematically investigated via experiments and crystal plasticity finite element model(CPFEM)simulation.The experimental results showed that the steel showed optimal strain hardenability and strength–plasticity matching when the bainite reached 35%.The 3D-CPFEM models with the same grain size and texture characters were established to clarify the influence of stress/strain distribution on PF+B dual phase steel with different bainite contents.The simulation results indicated that an appropriate increase in the bainite content(18%–35%)did not affect the interphase strain difference,but increased the stress distribution in both phases,as a result of enhancing the coordinated deformability of two phases and improving the strength–plasticity matching.When the bainite content increased to 53%,the stress/strain difference between the two phases was greatly increased,and plastic damage between the two phases was caused by the reduction of the coordinated deformability.
文摘The changes of proteins in the rice (Oryzasativa L.) Tesanai 2 seedling under salt (NaCl,4 g/L), heat shock (42℃, 3h ), and cold(14℃, 3d ) pretreatments were compared toexplore the mechanism of the cross adaptationto different environmental stresses. The chill-ing resistance of rice seedling after 1℃, 150pmol·msPFD(photo flux density) for 2d was enhanced distinctly by salt, heat shock,
文摘Volumetric fracturing is a primary stimulation technology for economical and effective exploitation of tight oil reservoirs. The main mechanism is to connect natural fractures to generate a fracture network system which can enhance the stimulated reservoir volume. By using the combined finite and discrete element method, a model was built to describe hydraulic fracture propagation in tight oil reservoirs. Considering the effect of horizontal stress difference, number and spacing of perforation clus- ters, injection rate, and the density of natural fractures on fracture propagation, we used this model to simulate the fracture propagation in a tight formation of a certain oil- field. Simulation results show that when the horizontal stress difference is lower than 5 MPa, it is beneficial to form a complex fracture network system. If the horizontal stress difference is higher than 6 MPa, it is easy to form a planar fracture system; with high horizontal stress differ- ence, increasing the number of perforation clusters is beneficial to open and connect more natural fractures, and to improve the complexity of fracture network and the stimulated reservoir volume （SRV）. As the injection rate increases, the effect of volumetric fracturing may be improved; the density of natural fractures may only have a great influence on the effect of volume stimulation in a low horizontal stress difference.
基金Supported by the China National Science and Technology Major Project(2016ZX05060001-032)
文摘The main area of the Jiaoshiba anticline of the Fuling shale gas field was taken as the research object,laboratory rock mechanical experiments and direct shear experiments were conducted to clarify the mechanical anisotropy characteristics and parameters of rock samples with rich beddings.Based on the experimental results,a 3D fracture propagation model of the target reservoir taking mechanical anisotropy,weak bedding plane and vertical stress difference into account was established by the discrete element method to analyze distribution patterns of hydraulic fractures under different bedding densities,mechanical properties,and fracturing engineering parameters(including perforation clusters,injection rates and fracturing fluid viscosity).The research results show that considering the influence of the weak bedding plane and longitudinal stress difference,the interlayer stress difference 3–4 MPa in the study area can control the fracture height within the zone of stress barrier,and the fracture height is less than 40 m.If the influence of the weak bedding plane is not considered,the simulation result of fracture height is obviously higher.Although the opening of high-density bedding fractures increases the complexity of hydraulic fractures,it significantly limited the propagation of fracture height.By reducing the number of clusters,increasing the injection rate,and increasing the volume and proportion of high-viscosity fracturing fluid in the pad stage,the restriction on fracture height due to the bedding plane and vertical stress difference can be reduced,and the longitudinal propagation of fractures can be promoted.The fracture propagation model was used to simulate one stage of Well A in Fuling shale gas field,and the simulation results were consistent with the micro-seismic monitoring results.
基金financially supported by the National Natural Science Foundation of China(Nos.21474111,21222407 and 21274152)subsidized by the National Basic Research Program of China(973 Program,2012CB821500)
文摘The structure and rheological properties of carbon-based particle suspensions, i.e., carbon black（CB）, multi-wall carbon nanotube（MWNT）, graphene and hollow carbon sphere（HCS） suspended in polydimethylsiloxane（PDMS）, are investigated. In order to study the effect of particle shape on the structure and rheological properties of suspensions, the content of surface oxygen-containing functional groups of carbon-based particles is controlled to be similar. Original spherical-like CB（fractal filler）, rod-like MWNT and sheet-like graphene form large agglomerates in PDMS, while spherical HCS particles disperse relatively well in PDMS. The dispersion state of carbon-based particles affects the critical concentration of forming a rheological percolation network. Under weak shear, negative normal stress differences（ΔN） are observed in CB, MWNT and graphene suspensions, while ΔN is nearly zero for HCS suspensions. It is concluded that the vorticity alignment of CB, MWNT and graphene agglomerates under shear results in the negative ΔN. However, no obvious structural change is observed in HCS suspension under weak shear, and accordingly, the ΔN is almost zero.
基金National Major Science&Technology Project“Stimulation technology for shale gas reservoirs”(No.2011ZX05018-004).
文摘Based on the research results of lab triaxial hydraulic fracturing simulation experiments,field fracturing practice,and theory analysis,the factors affecting the growth of hydraulic fracture network in shale reservoirs,including brittleness,difference of horizontal stress,distribution and mechanical characteristics of natural fractures,fluid viscosity and fracturing parameters,etc are analyzed in this study.The results show that the growth of fracture network in shale reservoirs is affected by geological factors and engineering factors jointly.From the perspective of reservoir geological factors,the higher the rock brittleness,the more developed the natural fractures,and the poorer the natural fracture consolidation,the more likely hydraulic fracture network will be formed.From the perspective of fracturing engineering factors,lower fluid viscosity and larger fracturing scale will be more helpful to the formation of extensive fracture network.On the basis of the analysis of single factors,a multi-factor coupling index has been established to characterize the growth degree of hydraulic fracture network and evaluate the complexity of hydraulic fractures after the fracturing of shale reservoirs.
基金supported by the National Science and Technology Major Project“Shale Gas Enrichment Law and Key Target Evaluation in Sichuan Basin and Periphery”(No.2017ZX05035-001)Hubei Province Colleges and Universities Excellent Young and Middle-aged Science and Technology Innovation Team Project“Shale Gas Formation Mechanism and Reservoir Evaluation”(No.T201905)+1 种基金Hubei Provincial Natural Science Foundation project“Study on the temporal and spatial distribution and paleogeography of organic-rich shale in the Wufeng-Longmaxi Formation in Western Hubei”(No.2018CFB330)“Research on the Lower Paleozoic High in Southwest Hubei-Development and evolution of organic pores in overmature marine shale”(No.2016CFB601).
文摘The Upper Ordovician Wufeng Formation and the Lower Silurian Longmaxi Formation in the central Yangtze area of southern China has a good prospect of shale gas exploration.So far,however,its complicated geological conditions and shale gas exploration and development potentials have not been understood completely,which affects its exploration achievements.In order to determine the main factors controlling shale gas enrichment in the WufengeLongmaxi Fms in this area,this paper studied the key shale gas enrichment conditions in the black shale there(e.g.sedimentary environments,reservoir development characteristics and preservation conditions)based on single-well comprehensive reservoir evaluation,combined with drilling,logging,core,outcrop and test data.In addition,it was comparedwith the main shale gas blocks in the Sichuan Basin,such as Jiaoshiba and Changning.And the following research results are obtained.First,the organic-rich shale section in this areawas formed during the sedimentation fromWufeng Formation to the third submember of the first member of Longmaxi Formation.It is the deposit of deepwater continental facies,and its thickness is in a range of 15-39 m,presenting a trend of increasing from south to north.Second,the reservoir rocks in the high-quality shale sections are dominated by siliceous shale,and the reservoir porosity is in a range of 1.60-7.44%.The reservoir spaces are dominated by organic pores with good connectivity and high total organic carbon(TOC)content.The organic matter is of a sapropel-sapropel prone hybrid type,with a high thermal evolution degree,better gas bearing property and good fracturability.Third,the Dangyang synclinorium is characterized by better strata preservation,rock occurrence flat,less developed faults and thick and stable roofs and floors,so its shale gas preservation conditions are better.Fourth,different from the Changning and Jiaoshiba Blocks,the central Yangtze area is characterized by great burial depth,large bidirectional stress difference,and low formation pressure coefficient.In conclusion,the WufengeLongmaxi Fms is better in shale gas enrichment conditions and has a potential of further exploration.However,its commercial shale gas development and scale production increase in the future face challenges due to its large stress difference,great burial depth,and lower pressure coefficient.
文摘To prevent dense chromium oxide covers from cracking during firing,such as internal cracking,surface cracking and fracture,the firing process of dense chromium oxide products was simulated,and the performance parameters at several specific temperatures before and after densification of chromium oxide green bodies were tested.The temperature difference and the stress difference of chromium oxide green bodies were calculated by Finite Element Software at heating and cooling rates of 10,20,30 and 40℃·h^(-1),respectively.The results show that large temperature difference and thermal stress difference are the main causes of internal cracking,surface cracking and fracture of the brick.Suitable heating rates reduce or avoid internal cracking of the brick.When the heating rate is 30℃·h^(-1) below 1450℃and 10℃·h^(-1) above 1450℃,the temperature difference and the thermal stress difference in the brick are below 4℃and 4 MPa,respectively,and there is no internal crack in the brick after firing.The initial cooling stage is the key stage that causes cracking or fracture of bricks.When the cooling rate is 20℃·h^(-1) above 1450℃,the thermal stress difference in the brick is less than 4 MPa,which can significantly reduce the surface cracks and fracture of the brick.
基金Project supported by the National Natural Science Foundation of China,“Microscopic Damage Mechanism of Saturated Brittle Rock during Blasting Induced Fracturing and Its Control Law on Macroscopic Damage”(No.51874339)the Natural Science Foundation of Shandong Province,“Study on the Coupled Response Mechanism of Crack Propagation and Rock Damage in the fracturing condition of multistage strong pulse”(No.ZR2016EEQ04)the Fundamental Research Funds for the Central Universities,China,“Study on the Mechanical Mechanism of Multistage Blasting Induced SRV in Deep-Seated Tight Reservoirs with High Horizontal Stress Difference”(No.17CX05004).
文摘For figuring out the stress interference of multiphase fracture combinations and its effect on the fracture initiation pressure of subsequent hydraulic fracturing,a calculation model for the coupled stress field with multiple induced fractures preexisted was established based on the calculation model for the stress field with single induced fracture preexisted,and the change laws of circumferential stress field around the wellbore under the effect of induced stress were analyzed.Then,the fracture initiation pressure of subsequent hydraulic fracturing was calculated according to the fracturing mechanics criterion.Finally,the effects of the length,phase,horizontal principal stress difference co-efficient and quantity of preexisting fractures on its initiation pressure were analyzed.And the following research results were obtained.First,the circumferential stress difference in the area near the fractures behind the preexisting fractures increase greatly and even the horizontal principal stress is reversed.Second,as the length of new preexisting fractures increases,the initiation pressure of initial preexisting fractures rises first and then drops.And when the length of new preexisting fractures is equal to that of the initial preexisting fractures,the initiation pressure of initial preexisting fractures drops quickly first and then slowly.Third,the initiation pressure of high phase fractures is lower than that of low phase fractures.Fourth,with the increase of fracture quantity,the initiation pressure of new preexisting fractures drops gradually,but the decline trend of initiation pressure difference is not obvious.Fifth,the stress interference of multiphase fractures influences the fracture initiation pressure,and high-phase long fractures and low-phase short fractures are both favorable for the simultaneous initiation of multiphase fractures.Sixth,syn-chronous propagation of multiple fractures can generate more complicated stress interference and excite the evolution of hydraulic fracture network,so as to realize uniform fracturing stimulation.In conclusion,the research results can provide a theoretical guidance for the design of fracturing operation,e.g.perforation phase of blasting induced fracturing,scale of induced fractures,and pump pressure of subsequent hydraulic fracturing.
基金supported by the State Key Laboratory of Coal Resources and Sate Mining,China University of Mining and Technology (No.SKLCRSM13X07)the National Natural Science Foundation of China (No.51174195)+1 种基金Chinese National Programs for Fundamental Research and Development (No.2013CB227900)the Fundamental Research Funds for the Central Universities (No.2014XT01)
文摘A reasonable unsupported roof distance(URD) when advancing underground coal mine roadways can contribute greatly to safe and rapid roadway development.A mechanical model of the roof,using the relationship between the roof stress distribution and URD,obtained by the difference method,and roof stability according to the in-situ roof stress and rock mass strength was developed.We subsequently designed a proper range of URD,developed a testing method of URD with the function of mining protection,evaluated roof stability through analyzing the test data and then determined a reasonable URD.Considering the factors of the geological conditions,the immediate roof stability and the efficiency of the labor arrangement system,the URD of the advancing roadway of 9802 working face in Zhangshuanglou coal mine was determined to be 6 m using the proposed method.The results show that,when a 2 m length of roadway was reinforced by temporary support and high pre-stressed bolt support after the roadway advancement of 6 m per cycle,the speed and the security of the roadway development can be achieved and the advance rate can reach more than 400 m per month.
文摘The main reservoir stratum of shale gas in the Jiaoshiba area, Sichuan basin is Wufeng-Longmaxi Formations. This paper calculates the tectonic difference stress values of JY1 well in different depths and numerically simulates the shale gas reservoir by elasto-plastic incremental method. The results of tectonic difference stress value suggest that the present tectonic stress values distribute strippedly in plane. The areas in favor of the development of fractures are stripped and mostly distributed in the northeast, middle and southwest of Jiaoshiba area, where the shale gas is concentrated.
