[Objective] The aim was to isolate the triazophos-degrading strain and study its degradation characteristics. [Method] A triazophos-degrading bacterium strain C-Y106 was isolated from sludge in an aeration tank of tri...[Objective] The aim was to isolate the triazophos-degrading strain and study its degradation characteristics. [Method] A triazophos-degrading bacterium strain C-Y106 was isolated from sludge in an aeration tank of triazophos manufacture. Then the strain C-Y106 was identified according to the morphology,physiological and biochemical characteristics,and 16S rRNA sequence analysis. The effect of medium with different nutrients on triazophos-degrading rate by C-Y106 was studied. [Result] The strain C-Y106 was identified as Bacillus subtilis. The strain C-Y106 could grow in the mineral salt medium with 40 mg/L of triazophos as the sole sources of carbon,Nitrogen and Phosphorus. The triazophos-degrading rate was the highest as 76.8% in the mineral salt medium with 40 mg/L of triazophos as the sole source of Phosphorus,after being incubated at 31 ℃,pH 8.0 and 150 r/min for 60 h. [Conclusion] The research had provided theoretical basis for the identification and purification of enzymes for triazophos degradation.展开更多
A comparative analysis of the codon usage bias was conducted in Methanosarcina mazei str. Goel and two related Euryarchaeota microorganisms （Picrophilus torridus str. DSM 9790 and Natronomonas pharaonis str. DSM 2160...A comparative analysis of the codon usage bias was conducted in Methanosarcina mazei str. Goel and two related Euryarchaeota microorganisms （Picrophilus torridus str. DSM 9790 and Natronomonas pharaonis str. DSM 2160）. Results revealed that synonymous codon usage in Methanosarcina mazei str. Goel was less biased, which was highly correlated with the GC3S value. And the codon usage patterns were phylogenetically conserved among those Euryarchaeota microorganisms. By employing a hierarchical clustering analysis, it can be seen that it is more the species than the gene function that determines their gene codon usage pattems. Considering that those microorganisms live in different environments where the pH conditions vary quite a lot, it can be presumed that their living environments, especially the pH conditions, play an important role in determining those microorganisms＇ codon usage pattems.展开更多
Y染色体短串联重复(Y chromosome short tandem repeat,Y-STR)在法庭科学实战应用中具有举足轻重的地位,基于Y-STR的长度分型排查嫌疑人父系谱系的技术已在多起重特大刑事案件的侦破中发挥了至关重要的作用。但当遇到长度多态Y-STR单倍...Y染色体短串联重复(Y chromosome short tandem repeat,Y-STR)在法庭科学实战应用中具有举足轻重的地位,基于Y-STR的长度分型排查嫌疑人父系谱系的技术已在多起重特大刑事案件的侦破中发挥了至关重要的作用。但当遇到长度多态Y-STR单倍型相近或者相同的数据时,难以进行谱系的精细化区分。基于二代测序技术解析Y-STR的序列信息为解决上述困境带来了新的路径,并已成功应用于实际案例。然而,当前Y-STR序列特征的基础数据积累显著不足,序列多态性的研究也较为零散,缺乏对Y-STR序列变异的系统性总结,在一定程度上限制了Y-STR序列多态性在法庭科学的深度应用。本文基于文献公开数据,归纳在法庭科学中应用较广泛的41个Y-STR基因座重复区及侧翼区的序列特征,梳理Y-STR基因座重复区和侧翼区内可定义单倍群的单核苷酸多态性,并展望序列多态性可以拓展应用的场景,以期为利用Y-STR序列多态性精细化区分男性谱系、溯源父系生物地理祖先等研究提供参考。展开更多
目的:调查四川地区北川羌族和西昌彝族两个少数民族群体中19个X-STRs基因座的遗传多态性,评估其法医学应用价值,并分析其群体结构。方法:收集北川羌族人群无关个体246例(男性188例,女性58例)和西昌彝族人群无关个体281例(男性116例,女性...目的:调查四川地区北川羌族和西昌彝族两个少数民族群体中19个X-STRs基因座的遗传多态性,评估其法医学应用价值,并分析其群体结构。方法:收集北川羌族人群无关个体246例(男性188例,女性58例)和西昌彝族人群无关个体281例(男性116例,女性165例)干血斑样本,采用Microreader^(TM)19X ID System kit直接扩增干血斑样本,ABI3500遗传分析仪对PCR产物进行毛细管电泳,GeneMapper■ ID-X软件自动分型。结果:在北川羌族群体中,共检测到150个等位基因,频率范围0.0033~0.8289;在西昌彝族群体中,共检测到164个等位基因,频率范围0.0022~0.8004。经统计学检验,两个少数民族群体基因频率分布均符合Hardy-Weinberg平衡定律(P>0.05),经Bonferroni校正后,不存在连锁不平衡的基因座。在北川羌族群体中,男性个体识别能力(PDM)和女性个体识别能力(PDF)分别是0.999999999945624、0.99999999999999999322,二联体和三联体累积平均排除概率分别是0.999999591813975、0.999999999465296;在西昌彝族群体中,男性个体识别能力(PDM)和女性个体识别能力(PDF)分别为0.999999999873529、1;二联体和三联体累积平均排除概率分别是0.999999284224924、0.999999998931424。结论:Microreader TM 19X ID荧光检测试剂盒在北川羌族和西昌彝族人群中总体多态性较高,鉴别能力较好,可作为亲权关系和个体识别有力的补充和验证手段。展开更多
文摘[Objective] The aim was to isolate the triazophos-degrading strain and study its degradation characteristics. [Method] A triazophos-degrading bacterium strain C-Y106 was isolated from sludge in an aeration tank of triazophos manufacture. Then the strain C-Y106 was identified according to the morphology,physiological and biochemical characteristics,and 16S rRNA sequence analysis. The effect of medium with different nutrients on triazophos-degrading rate by C-Y106 was studied. [Result] The strain C-Y106 was identified as Bacillus subtilis. The strain C-Y106 could grow in the mineral salt medium with 40 mg/L of triazophos as the sole sources of carbon,Nitrogen and Phosphorus. The triazophos-degrading rate was the highest as 76.8% in the mineral salt medium with 40 mg/L of triazophos as the sole source of Phosphorus,after being incubated at 31 ℃,pH 8.0 and 150 r/min for 60 h. [Conclusion] The research had provided theoretical basis for the identification and purification of enzymes for triazophos degradation.
文摘A comparative analysis of the codon usage bias was conducted in Methanosarcina mazei str. Goel and two related Euryarchaeota microorganisms （Picrophilus torridus str. DSM 9790 and Natronomonas pharaonis str. DSM 2160）. Results revealed that synonymous codon usage in Methanosarcina mazei str. Goel was less biased, which was highly correlated with the GC3S value. And the codon usage patterns were phylogenetically conserved among those Euryarchaeota microorganisms. By employing a hierarchical clustering analysis, it can be seen that it is more the species than the gene function that determines their gene codon usage pattems. Considering that those microorganisms live in different environments where the pH conditions vary quite a lot, it can be presumed that their living environments, especially the pH conditions, play an important role in determining those microorganisms＇ codon usage pattems.
文摘Y染色体短串联重复(Y chromosome short tandem repeat,Y-STR)在法庭科学实战应用中具有举足轻重的地位,基于Y-STR的长度分型排查嫌疑人父系谱系的技术已在多起重特大刑事案件的侦破中发挥了至关重要的作用。但当遇到长度多态Y-STR单倍型相近或者相同的数据时,难以进行谱系的精细化区分。基于二代测序技术解析Y-STR的序列信息为解决上述困境带来了新的路径,并已成功应用于实际案例。然而,当前Y-STR序列特征的基础数据积累显著不足,序列多态性的研究也较为零散,缺乏对Y-STR序列变异的系统性总结,在一定程度上限制了Y-STR序列多态性在法庭科学的深度应用。本文基于文献公开数据,归纳在法庭科学中应用较广泛的41个Y-STR基因座重复区及侧翼区的序列特征,梳理Y-STR基因座重复区和侧翼区内可定义单倍群的单核苷酸多态性,并展望序列多态性可以拓展应用的场景,以期为利用Y-STR序列多态性精细化区分男性谱系、溯源父系生物地理祖先等研究提供参考。
文摘目的:调查四川地区北川羌族和西昌彝族两个少数民族群体中19个X-STRs基因座的遗传多态性,评估其法医学应用价值,并分析其群体结构。方法:收集北川羌族人群无关个体246例(男性188例,女性58例)和西昌彝族人群无关个体281例(男性116例,女性165例)干血斑样本,采用Microreader^(TM)19X ID System kit直接扩增干血斑样本,ABI3500遗传分析仪对PCR产物进行毛细管电泳,GeneMapper■ ID-X软件自动分型。结果:在北川羌族群体中,共检测到150个等位基因,频率范围0.0033~0.8289;在西昌彝族群体中,共检测到164个等位基因,频率范围0.0022~0.8004。经统计学检验,两个少数民族群体基因频率分布均符合Hardy-Weinberg平衡定律(P>0.05),经Bonferroni校正后,不存在连锁不平衡的基因座。在北川羌族群体中,男性个体识别能力(PDM)和女性个体识别能力(PDF)分别是0.999999999945624、0.99999999999999999322,二联体和三联体累积平均排除概率分别是0.999999591813975、0.999999999465296;在西昌彝族群体中,男性个体识别能力(PDM)和女性个体识别能力(PDF)分别为0.999999999873529、1;二联体和三联体累积平均排除概率分别是0.999999284224924、0.999999998931424。结论:Microreader TM 19X ID荧光检测试剂盒在北川羌族和西昌彝族人群中总体多态性较高,鉴别能力较好,可作为亲权关系和个体识别有力的补充和验证手段。
