Understanding livestock performance in typical steppe ecosystems is essential for optimizing grassland-livestock interactions and minimizing environmental impact.To assess the effects of different stocking rates on th...Understanding livestock performance in typical steppe ecosystems is essential for optimizing grassland-livestock interactions and minimizing environmental impact.To assess the effects of different stocking rates on the growth performance,energy and nitrogen utilization,methane(CH_(4))emissions,and grazing behavior of Tan sheep,a 2-year grazing experiment in the typical steppe was conducted.The grazing area was divided into 9 paddocks,each 0.5 ha,with 3 spatial replicates for each stocking rate treatment(4,8,and 13 sheep per paddock),corresponding to 2.7,5.3,and 8.7 sheep ha^(–1).The results showed that the neutral detergent fiber(NDF)and acid detergent fiber(ADF)contents of herbage varied between grazing years(P<0.05),with a positive correlation between stocking rate and crude fiber content in the herbage(P<0.05).Dry matter intake(DMI)decreased with increasing stocking rate(P<0.05),and the average daily gain(ADG)was highest at 2.7 sheep ha^(–1)(P<0.05).Compared to 2.7 and 8.7 sheep ha^(–1),the5.3 sheep ha^(–1)treatment exhibited the lowest nutrient digestibility for dry matter,nitrogen,and ether extract(P<0.05).Fecal nitrogen was lowest at 8.7 sheep ha^(–1)(P<0.05),while retained nitrogen as a proportion of nitrogen intake was highest.Digestive energy(DE),metabolic energy(ME),and the ratios of DE to gross energy(GE)and ME to GE were highest at 8.7 sheep ha^(–1)(P<0.05).In contrast,CH_4 emissions,CH_4 per DMI,and CH_(4)E as a proportion of GE were highest at 2.7 sheep ha^(–1)(P<0.05).Stocking rate and grazing year did not significantly affect rumen fermentation parameters,including volatile fatty acids,acetate,propionate,and the acetate/propionate ratio.At 8.7sheep ha^(–1),daily grazing time and inter-individual distance increased,while time allocated to grazing,walking,and ruminating/resting decreased as stocking rates increased(P<0.05).This study highlights the importance of adjusting stocking rates based on the nutritional value of forage and grazing year to optimize grazing management.展开更多
Driven by the accelerating global aging population and increasing health consciousness, compression stocking market is experiencing rapid growth. According to the latest data from Grand View Research, the global compr...Driven by the accelerating global aging population and increasing health consciousness, compression stocking market is experiencing rapid growth. According to the latest data from Grand View Research, the global compression therapy market reached $4.25 billion in 2024, with medical compression stockings, as a core segment, boasting a compound annual growth rate(CAGR) of nearly 6%.展开更多
Hatchery release is a common and effective practice for protecting and restoring wild resources,and the success of the practice is commonly assessed using mark-recapture technology.We investigated the use of different...Hatchery release is a common and effective practice for protecting and restoring wild resources,and the success of the practice is commonly assessed using mark-recapture technology.We investigated the use of different dimensional X-ray imaging techniques for the strontium(Sr)marking of fish fin rays for stocking.Megalobrama amblycephala juveniles were marked by culturing specimens in 800-mg/L SrCl_(2)·6 H_(2)O solution,the cross-sections of dorsal fin rays were subsequently obtained,and the concentrations Sr was analyzed by 2 D imaging using an electron probe X-ray microanalyzer.Our preliminary findings indicate that the immersion marking method is effective for the Sr marking of fin rays in experimental fish.Moreover,we generated a bird’s-eye-view 3 D mesh image of the Sr concentrations,which can provide a more comprehensive information for fish stocking than that using normal 2 D imaging.展开更多
Optimal stocking densities were investigated for the sea cucumber Apostichopusjaponicus Selenka under feed-supplement and non-feed-supplement regimes in net enclosures for 333 d. Substantial weight loss occurred durin...Optimal stocking densities were investigated for the sea cucumber Apostichopusjaponicus Selenka under feed-supplement and non-feed-supplement regimes in net enclosures for 333 d. Substantial weight loss occurred during the aestivation phase （AE）. Decreased growth rates were also observed during the winter phase （WT）. In contrast, sea cucumbers showed rapid growth during the spring （SP） and autumn （AU） phases. Feeding regimes considerably influenced the growtb performance, i.e., sea cucumbers grew faster under feed-supplement regime than under non-feed-supplement regime （P 〈 0.05）. The average survival rates of sea cucumbers under feed-supplement regime were higher than those under non-feed-supplement regime for both the autumn phase and spring phase, but the differences were only significant for the latter phase （P〈 0.05）. The fitted B-N curves showed that the optimal stocking densities, in terms of net production, were 22.3 ind. m^-2 for feed-supplement regime and 14.1 ind. m^-2 for non-feed-supplement regime.展开更多
Atlantic salmon Salmo salar were reared at four stocking densities--high density D1 （final density -39 kg/m^3）, medium densities DE （-29 kg/m^3） and D3 （~19 kg/m^3）, and low density D4 （-12 kg/m^3）- for 40 day...Atlantic salmon Salmo salar were reared at four stocking densities--high density D1 （final density -39 kg/m^3）, medium densities DE （-29 kg/m^3） and D3 （~19 kg/m^3）, and low density D4 （-12 kg/m^3）- for 40 days to investigate the effect of stocking density on their growth performance, body composition and energy budgets. Stocking density did not significantly affect specific growth rate in terms of weight （SGRw） but did affect specific growth rate in terms of energy （SGRe）. Stocking density significantly influenced the ration level （RLw and RLe）, feed conversion ratio （FCRw and FCRe） and apparent digestibility rate （ADR）. Ration level and FCRw tended to increase with increasing density. Fish at the highest density D~ and lowest density D4 showed lower FCRe and higher ADR than at medium densities. Stocking density significantly affected protein and energy contents of the body but did not affect its moisture, lipid, or ash contents. The expenditure of energy for metabolism in the low-density and high-density groups was lower than that in the medium-density groups. Stocking density affected energy utilization from the feces but had no effect on excretion rate. The greater energy allocation to growth at high density and low density may be attributed to reduced metabolic rate and increased apparent digestibility rate. These findings provide information that will assist selection of suitable stocking densities in the Atlantic-salmon-farming industry.展开更多
Limited information has been available about the influence of loading density on the performances of Scophthalmus maximus, especially in recirculating aquaculture systems （RAS）. In this study, turbot （13.84±2....Limited information has been available about the influence of loading density on the performances of Scophthalmus maximus, especially in recirculating aquaculture systems （RAS）. In this study, turbot （13.84±2.74 g; average weigh±SD） were reared at four different initial densities （low 0.66, medium 1.26, sub-high 2.56, high 4.00 kg/m^2） for 10 weeks in RAS at 23±1℃ Final densities were 4.67, 7.25, 14.16, and 17.47 kg/m^2, respectively, which translate to 82, 108, 214, and 282 percent coverage of the tank bottom. Density had both negative and independent impacts on growth. The final mean weight, specific growth rate （SGR）, and voluntary feed intake significantly decreased and the coefficient of variation （CV） of final body weight increased with increase in stocking density. The medium and sub-high density groups did not differ significantly in SGR, mean weight, CV, food conversion rate （FCR）, feed intake, blood parameters, and digestive enzymes. The protease activities of the digestive tract at pH 7, 8.5, 9, and 10 were significantly higher for the highest density group, but tended to be lower （not significantly） at pH 4 and 8.5 for the lowest density group. The intensity of protease activity was inversely related to feed intake at the different densities. Catalase activity was higher （but not significantly） at the highest density, perhaps because high density started to induce an oxidative effect in turbot. In conclusion, turbot can be cultured in RAS at a density of less than 17.47 kg/m^2. With good water quality and no feed limitation, initial density between 1.26 and 2.56 kg/m^2 （final： 7.25 and 14.16 kg/m^2） would not negatively affect the turbot cultured in RAS. For culture at higher density, multi-level feeding devices are suggested to ease feeding competition.展开更多
Background:High stocking density(HSD)stress has detrimental effects on growth performance,intestinal barrier function,and intestinal microbiota in intensive animal production.Organic acids(OA)are widely used as feed a...Background:High stocking density(HSD)stress has detrimental effects on growth performance,intestinal barrier function,and intestinal microbiota in intensive animal production.Organic acids(OA)are widely used as feed addi-tives for their ability to improve growth performance and intestinal health in poultry.However,whether dietary OA can ameliorate HSD stress-induced impaired intestinal barrier in broilers remains elusive.In this study,a total of 528 one-day-old male Arbor Acres broilers were randomly allocated into 3 treatments with 12 replicates per treatment including 10 birds for normal stocking density and 17 birds for HSD.The dietary treatments were as follows:1)Normal stocking density+basal diet;2)HSD+basal diets;3)HSD+OA.Results:HSD stress can induce increased levels of serum corticosterone,lipopolysaccharides,interleukin-1β,tumor necrosis factor-α,and down-regulated mRNA expression of ZO-1,resulting in compromised growth performance of broilers(P<0.05).Dietary OA could significantly reduce levels of serum corticosterone,lipopolysaccharides,interleukin-1β,and tumor necrosis factor-α,which were accompanied by up-regulated interleukin-10,mRNA expres-sion of ZO-1,and growth performance(P<0.05).Moreover,OA could down-regulate the mRNA expression of TLR4 and MyD88 to inhibit the NF-κB signaling pathway(P<0.05).Additionally,HSD stress significantly decreased the abundance of Bacteroidetes and disturbed the balance of microbial ecosystems,whereas OA significantly increased the abundance of Bacteroidetes and restored the disordered gut microbiota by reducing competitive and exploita-tive interactions in microbial communities(P<0.05).Meanwhile,OA significantly increased the content of acetic and butyric acids,which showed significant correlations with intestinal inflammation indicators(P<0.05).Conclusions:Dietary OA ameliorated intestinal inflammation and growth performance of broilers through restor-ing the disordered gut microbial compositions and interactions induced by HSD and elevating short-chain fatty acid production to inhibit the TLR4/NF-κB signaling pathway.These findings demonstrated the critical role of intestinal microbiota in mediating the HSD-induced inflammatory responses,contributing to exploring nutritional strategies to alleviate HSD-induced stress in animals.展开更多
Background： Highly automated cage-rearing systems are becoming increasingly popular in China. However, a high stocking density can cause oxidative stress and decrease broiler performance. The tryptophan （TRP） deriv...Background： Highly automated cage-rearing systems are becoming increasingly popular in China. However, a high stocking density can cause oxidative stress and decrease broiler performance. The tryptophan （TRP） derivative 5-hydroxytryptophan （5-HT） has been shown to preserve membrane fluidity in birds suffering from oxidative stress Therefore, this experiment was conducted to determine the effects of dietan/TRP supplementation on performance, breast meat quality and oxidative stress in broilers reared in cages with a high or low stocking density. Methods： Female Arbor Acres broilers （25-d-old, n = 144） were randomly allocated to 1 of 4 treatments. The birds were fed a diet based on corn, soybean meal, cottonseed meal and corn gluten meal containing either 0.18 or 0.27% TRP and were housed with stocking densities of 11 or 15.4 birds/m2 in a 2 x 2 factorial experiment. Broiler performance was evaluated from d 25 to 42. Eight birds from each treatment were slaughtered on d 42 and plasma and breast muscle samples were collected to measure biochemical indices. Results： A higher stocking density tended to be associated with reduced weight gain （P 〈 0.10）, and significantly increased plasma glutamic-pyruvic transaminase （GPT） activity （P 〈 0.001）. Increased dietary TRP significantly reduced the activities of lactic dehydrogenase and GPT while increasing total cholesterol in the plasma （P 〈 0.01）, reducing drip loss of breast muscle （P 〈 0.10） and improving feed efficiency （P 〈 0.10）. Conclusions： An increase in dietary TRP, ].S-fold higher than the standard supplementation level, can alleviate oxidative stress as well as improve welfare and feed efficiency in broilers reared in cages with a high stocking density.展开更多
Atlantic salmon reared in recirculating aquaculture system(RAS) may lead to inappropriately high stocking density, because ?sh live in a limited space. Finding the suitable stocking density of Atlantic salmon reared i...Atlantic salmon reared in recirculating aquaculture system(RAS) may lead to inappropriately high stocking density, because ?sh live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the in?uence of stocking density on growth and some stress related physiological factors were investigated to evaluate the ef fects of stocking density. The ?sh were reared for 220 days at ?ve densities(A: 24 kg/m^3; B: 21 kg/m^3; C: 15 kg/m^3; D: 9 kg/m^3 and E: 6 kg/m^3). The results show that 30 kg/m^3 might be the maximum density which RAS can af ford in China. The stocking densities under 30 kg/m^3 have no ef fect on mortality of Atlantic salmon reared in RAS. However, the speci?c growth rate(SGR), ?nal weight and weight gain in the high density group were signi?cantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate(FCR) had a negative correlation with density. Plasma hormone T_3 and GH showed signi?cant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone(T 3), GH(growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT(glutamic oxalacetic transaminase) and GPT(glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no ef fects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise effcient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.展开更多
The effects of stocking density on the growth and metabolism of Amur sturgeon were assessed. Amur sturgeon were grown for 70 days at three dif ferent stocking densities(low stocking density, LSD: 5.5 kg/m^3; medium st...The effects of stocking density on the growth and metabolism of Amur sturgeon were assessed. Amur sturgeon were grown for 70 days at three dif ferent stocking densities(low stocking density, LSD: 5.5 kg/m^3; medium stocking density, MSD: 8.0 kg/m^3; and high stocking density, HSD: 11.0 kg/m^3), and the biometric index, muscle composition, and serum biochemical parameters were evaluated. In addition, pituitary, liver, and muscle samples were collected for gene cloning and expression analyses. After 70 days of growth, the fish maintained at HSD had significantly lower fi nal body weight and specifi c growth rate, and a higher feed conversion ratio than those of the fish in the MSD and LSD groups. The HSD group had the lowest lipid and protein concentrations in serum and muscle. The serum cortisol concentration increased significantly in the HSD group, indicating that the stress-response system was activated in these fish. There was no change in the concentration of serum insulin-like growth factor 2(IGF-2), while the concentrations of serum growth hormone(GH) and insulin-like growth factor 1(IGF-1) decreased in the HSD group. The full-length cDNAs of G H and IGF-2 genes(995-bp and 1 207-bp long, respectively), were cloned and analyzed. In the HSD group, the expressions of GH in the pituitary and growth hormone receptor( GHR) and IGF-1 in the liver were down-regulated at the end of the 70-day experiment. In the HSD group, the transcript level of IGF-2 significantly decreased in the liver, but did not change in muscle. Overall, our results indicated that a HSD negatively af fects the growth performance and leads to changes in lipid and protein metabolism in Amur sturgeon. The down-regulated expression of genes related to the GH/IGF axis may be responsible for the poor growth performance of Amur sturgeon under crowding stress.展开更多
Hybrid tilapia were reared at densities of 1, 5 or 10 fish per tank for four weeks. Mortality was 0 at 1 and 10 fish per tank, but was 25% at 5 fish per tank. Specific growth rate was highest at 1 fish per tank, and l...Hybrid tilapia were reared at densities of 1, 5 or 10 fish per tank for four weeks. Mortality was 0 at 1 and 10 fish per tank, but was 25% at 5 fish per tank. Specific growth rate was highest at 1 fish per tank, and lowest at 5 fish per tank. The lower growth rate at the intermediate stocking density was associated with reduced feed efficiency, but there was no reduction in feed intake or digestibility. The results suggested increased metabolic cost caused by aggressive behaviour at intermediate stocking density, which can be suppressed by a further increase in density.展开更多
We investigate a stage-structured delayed predator-prey model with impulsive stocking on prey and continuous harvesting on predator. According to the fact of biological resource management, we improve the assumption o...We investigate a stage-structured delayed predator-prey model with impulsive stocking on prey and continuous harvesting on predator. According to the fact of biological resource management, we improve the assumption of a predator-prey model with stage structure for predator population that each individual predator has the same ability to capture prey. It is assumed that the immature and mature individuals of the predator population are divided by a fixed age, and immature predator population does not have the ability to attach prey. Sufficient conditions are obtained, which guarantee the global attractivity of predator-extinction periodic solution and the permanence of the system. Our results show that the behavior of impulsive stocking on prey plays an important role for the permanence of the system, and provide tactical basis for the biological resource management. Numerical analysis is presented to illuminate the dynamics of the system.展开更多
The Chinese soft-shelled turtle Pelodiscus sinensis is a high-valued freshwater species cultured in China.This study investigated the effects of stocking density on water quality,growth performance and economic return...The Chinese soft-shelled turtle Pelodiscus sinensis is a high-valued freshwater species cultured in China.This study investigated the effects of stocking density on water quality,growth performance and economic return of Pelodiscus sinensis cultured in ponds.P.sinensis were stocked at densities of 1 ind./m^2(LD:low stocking density) and 2 ind./m^2(HD:high stocking density) in 3 000-m^2 ponds,with three replicate ponds for each density.P.sinensis juveniles were fed with a commercial dry pellet feed of 46% crude protein and minced fillet of silver carp ans cultured for 122 days.The results showed that the levels of total nitrogen(TN),total phosphorous(TP),Chlorophyll-a(Chl.a) and turbidity in LD treatment were significantly lower than those in HD treatment(P<0.05).The mean TN and TP concentration in LD treatment was 29.3% and 35.7% lower compared to the HD treatment at the end of the experiment,respectively.Mean survival rates,final weight,average growth rates and PER were significantly higher in LD treatment compared with the HD treatment(P<0.05),respectively.Production was significantly affected by stocking density,which was higher in HD treatment,but the net income was higher in LD treatment.The results suggest that turtles with mean weight 55.6 g rearing at a low stocking density(1 ind./m^2) in ponds had a positive effect on overall economic return and was effective at improving turtle growth performance and water quality.展开更多
Stocking density is an important factor for animal production performance.It appears particularly urgent and important to research systematically on stocking density for the dual restriction of environmental protectio...Stocking density is an important factor for animal production performance.It appears particularly urgent and important to research systematically on stocking density for the dual restriction of environmental protection and animal welfare.This review dealt with the influence of stocking density on ducks'productivity and health through production performance,animal behavior and animal welfare,and analyzed the possible mechanism of high stocking density reducing production performance,resulting in abnormal behavior and stress,causing welfare and health problems.We consider that it might be associated with heat stress,more spatial competition(to gather the food,drinking water,sports area,etc.),as well as the quality deterioration of litter and house air caused by high stocking density.Finally,we put forward some suggestions on the study of stocking density's effects on ducks,and proposed the future work that need to be studied and problems that need to be solved imminently.展开更多
Stocking density is widely recognized as a critical factor in aquaculture and a potential source of long-term stress.The influence of stocking density on growth and stress response of juvenile turbot（Scophthalmus max...Stocking density is widely recognized as a critical factor in aquaculture and a potential source of long-term stress.The influence of stocking density on growth and stress response of juvenile turbot（Scophthalmus maximus, ~3–75g, initial to final weight） was examined in fish held under low（LD, ~0.21–5.31 kg/m^2, initial to final density）,medium（MD, ~0.42–10.81 kg/m^2） and high stocking density（HD, ~0.63–14.27 kg/m^2） for 120 days in a recirculating aquaculture system（RAS）. In this trial, the growth curve for weight of juvenile turbot in RAS, all fitted by the Schnute model. No significant difference was found in growth performance among the three densities until at the final sampling（Day 120）. The final weight and body weight increase（BWI） in the HD group were significantly lower than in other groups（P〈0.05, weight：（75.83±2.49） g,（75.39±2.08） g,（65.72±2.86） g and BWI：（2 436.12±28.10）%,（2 421.29±4.64）%,（2 097.88±20.99）% in LD, MD and HD groups, respectively）. Similarly, the specific growth rate（SGR）, feed conversion ratio（FCR） and coefficient of variation for weight（CV_w） were adversely affected by high stocking density（P〈0.05）. However, there was no difference in survival and Fulton＇s condition factor（K） of turbot among the different groups. Physiological analyses demonstrated a clear increase in the plasma cortisol level and an obvious decrease in growth hormone（GH） concentration in the HD group on Day120（P〈0.05）. There was no significant effect of stocking density on plasma glucose, Cl– and protein levels. All these findings would provide a reference for selecting the optimal stocking density of juvenile turbot in RAS.展开更多
The inadequate supply of tilapia fingerling is a major limitation to cage culture development in Ghana. Lake-based hapa systems are very efficient in nursing fingerlings although the process can be tricky due to inher...The inadequate supply of tilapia fingerling is a major limitation to cage culture development in Ghana. Lake-based hapa systems are very efficient in nursing fingerlings although the process can be tricky due to inherent effects of stocking densities and environmental factors. This study aimed at assessing the growth, survival and profitability of Nile tilapia (Oreochromis niloticus) fingerlings of the Akosombo strain reared in nine 1 m<sup>3</sup> Lake-based hapas at different densities. Each hapa was stocked with 2.12 ± 0.14 g sex-reversed tilapia fingerlings at varying stocking densities of 400 fish/m<sup>3</sup>, 800 fish/m<sup>3</sup> and 1200 fish/m<sup>3</sup> serving as treatments T1, T2 and T3 respectively. Each treatment was replicated thrice. Bi-weekly samplings were done and water quality parameters were measured. After the experiment, analysis of variance showed significant differences (p展开更多
The Pacific oyster Crassostrea gigas is one of the most widely cultivated aquaculture species and contributes signifi-cantly to total seafood production for human beings.However,mass mortality occurred frequently,and ...The Pacific oyster Crassostrea gigas is one of the most widely cultivated aquaculture species and contributes signifi-cantly to total seafood production for human beings.However,mass mortality occurred frequently,and in some regions almost all oysters died during seed production and grow-out stage.In order to explore whether hybridization breeding can improve its growth and survival,a complete diallel cross between a selected strain‘Haida No.1’(S)and an orange shell variant(O)of C.gigas was carried out.The larval growth and survival were compared among hybrids and purebred strains at temperatures of 16,20,24,28 and 32℃;salinities of 15,20,25,30 and 35;and stocking densities of 0.5,1,2,4 and 8 larvae mL−1.Under different environments,the hybridization between two strains of C.gigas showed the heterosis of growth and survival.The mean shell height and survival rate of the two reciprocal crosses(OS,SO)were significantly higher than those of the two purebred strains(SS,OO)under all environ-mental conditions.In particular,OS showed greater heterosis than the purebred strains and SO progeny.The results showed that the productive traits of the‘Haida No.1’could be improved by crossing with the orange shell line.Meanwhile,the results from this study also indicated that hybridization between the two strains of C.gigas may be a promising way for breeding new variety with high survival rate.展开更多
The combined effects of feeding rate(0.8%,1.0%,and 1.2%initial body weight/day),feeding frequency(two,three,and four times/day) and stocking density(10,15,and 20 kg/m3) in recirculating aquaculture systems(RAS) on gro...The combined effects of feeding rate(0.8%,1.0%,and 1.2%initial body weight/day),feeding frequency(two,three,and four times/day) and stocking density(10,15,and 20 kg/m3) in recirculating aquaculture systems(RAS) on growth performance,digestion and waste generation of Atlantic salmon(Salmo salar L.) were investigated in an 8-week orthogonal experiment(L9(3)3) with a constant daily water renewal at 7.50%of total volume.No mortality occurred during the experimental period.Feed conversion ratio(FCR) varied from 0.90 to 1.13 and specific growth rate(SGR) ranged from 0.48%to 0.69%/day.SGR,thermal growth coefficient(TGC) and FCR were not significantly(P>0.05) affected by the three factors,while net protein utilization(NPU) was significantly(P<0.05) affected.Apparent digestibility coefficients(ADC) of dry matter in the present study were in the range 66.12%-73.55%.ADC in protein,lipid and energy were statistically different among all treatments and in the range of 90.07%-93.67%,81.54%-89.15%,and67.55%-71.87%,respectively.The proportion of mean total ammonia nitrogen excreted ranged from 1.37%to 1.64%of feed nitrogen at steady state,and the concentration of nitrogenous and phosphorus compounds were differently correlated to the three factors.The results will provide valuable reference data for culture management decisions in the Atlantic salmon farming industry.展开更多
Juvenile turbot （Scophthalmus maximus） the initial stock densities at 0.28, 0.87, 1.12, 1.16, 2.75 were reared in five different experiment groups in kg/m^2 respectively for the study of the density effect on growth...Juvenile turbot （Scophthalmus maximus） the initial stock densities at 0.28, 0.87, 1.12, 1.16, 2.75 were reared in five different experiment groups in kg/m^2 respectively for the study of the density effect on growth, pigmentation and feed coefficient rate. The experiment lasted for 60 days with final stock densities at 1.91, 6.31, 8.86, 11.97, 17.67 kg/m^2 respectively. Result showed that, in the same experiment condition, the stocking density has a positive effect on growth in low density and negative in high density. The SGR （special growth rate） was 3.189, 3.304, 3.447, 3.341, and 3.087 respectively. The uniformity of weight distributing among experiment groups decreased with increasing density. The stocking density had positive effect on feed coefficient rate. Group 1 had the least feed coefficient rate 0.96, and highest at 1.25 in Group 5, the highest density group. High stocking density inhibited the growth and increased the feed coefficient rate. The stocking density had negative relationship to pigmentation improvement for whitened fish.展开更多
Silvicultural practices applied in managed forest plantations may help counteract the effects of climate change by influencing soil surface CO_(2)efflux(Fs).Understanding the effects of silvicultural practices on Fs w...Silvicultural practices applied in managed forest plantations may help counteract the effects of climate change by influencing soil surface CO_(2)efflux(Fs).Understanding the effects of silvicultural practices on Fs will provide unbiased estimates of carbon fluxes and allow better silvicultural decisions for carbon sequestration.Therefore,we assessed how Fs differed seasonally across silvicultural practices(i.e.,stocking levels,clone,fertilization and weed control treatments)and evaluated the effects of soil temperature(Ts)and soil volumetric water content(θv)on Fs across these practices for a mid-rotation(14 year-old)Pinus radiata plantation in the Canterbury region of New Zealand.There were significant differences in Fs(p<0.05)over the four seasons,three levels of stocking,and five clones.The effects of fertilization and weed control applied 12 years previously on Fs were insignificant.Annual estimate of Fs(mean±1 standard deviation)from the study site was 22.7±7.1 t ha^(-1)a^(-1)in the form of CO_(2)(6.2±2.1 t ha^(-1)a^(-1)in the form of C).Fs values were consistently higher in plots with 1250 stems ha^(-1)compared to 2500 stems ha^(-1),which may be related to a strong soil resource limitation because of the close spacing in the latter plantation.Significant differences in Fs across clones suggest that variations in carbon partitioning might explain their growth performance.Silvicultural treatments influenced Fs response to soil temperature(p<0.05),resulting in models explaining 28-49%of the total variance in Fs.These findings provide insights into how silvicultural management decisions may impact Fs in mid-rotation radiata pine plantations,contributing towards developing more precise and unbiased plantation carbon budgets.展开更多
基金supported by the National Natural Science Foundation of China(32161143028)the Key Technology of Grassland Ecological Civilization Demonstration Area in Ningxia Hui Autonomous Region,China(20210239)the Northwest Shelterbelt Construction Bureau of the National Forestry and Grassland Administration,China。
文摘Understanding livestock performance in typical steppe ecosystems is essential for optimizing grassland-livestock interactions and minimizing environmental impact.To assess the effects of different stocking rates on the growth performance,energy and nitrogen utilization,methane(CH_(4))emissions,and grazing behavior of Tan sheep,a 2-year grazing experiment in the typical steppe was conducted.The grazing area was divided into 9 paddocks,each 0.5 ha,with 3 spatial replicates for each stocking rate treatment(4,8,and 13 sheep per paddock),corresponding to 2.7,5.3,and 8.7 sheep ha^(–1).The results showed that the neutral detergent fiber(NDF)and acid detergent fiber(ADF)contents of herbage varied between grazing years(P<0.05),with a positive correlation between stocking rate and crude fiber content in the herbage(P<0.05).Dry matter intake(DMI)decreased with increasing stocking rate(P<0.05),and the average daily gain(ADG)was highest at 2.7 sheep ha^(–1)(P<0.05).Compared to 2.7 and 8.7 sheep ha^(–1),the5.3 sheep ha^(–1)treatment exhibited the lowest nutrient digestibility for dry matter,nitrogen,and ether extract(P<0.05).Fecal nitrogen was lowest at 8.7 sheep ha^(–1)(P<0.05),while retained nitrogen as a proportion of nitrogen intake was highest.Digestive energy(DE),metabolic energy(ME),and the ratios of DE to gross energy(GE)and ME to GE were highest at 8.7 sheep ha^(–1)(P<0.05).In contrast,CH_4 emissions,CH_4 per DMI,and CH_(4)E as a proportion of GE were highest at 2.7 sheep ha^(–1)(P<0.05).Stocking rate and grazing year did not significantly affect rumen fermentation parameters,including volatile fatty acids,acetate,propionate,and the acetate/propionate ratio.At 8.7sheep ha^(–1),daily grazing time and inter-individual distance increased,while time allocated to grazing,walking,and ruminating/resting decreased as stocking rates increased(P<0.05).This study highlights the importance of adjusting stocking rates based on the nutritional value of forage and grazing year to optimize grazing management.
文摘Driven by the accelerating global aging population and increasing health consciousness, compression stocking market is experiencing rapid growth. According to the latest data from Grand View Research, the global compression therapy market reached $4.25 billion in 2024, with medical compression stockings, as a core segment, boasting a compound annual growth rate(CAGR) of nearly 6%.
基金Supported by the National Key Research and Development Program of China(No.2022 YFF 0608203)the Graduate Student Scientific Research Innovation Projects in Jiangsu Province(No.KYCX 22-0706)。
文摘Hatchery release is a common and effective practice for protecting and restoring wild resources,and the success of the practice is commonly assessed using mark-recapture technology.We investigated the use of different dimensional X-ray imaging techniques for the strontium(Sr)marking of fish fin rays for stocking.Megalobrama amblycephala juveniles were marked by culturing specimens in 800-mg/L SrCl_(2)·6 H_(2)O solution,the cross-sections of dorsal fin rays were subsequently obtained,and the concentrations Sr was analyzed by 2 D imaging using an electron probe X-ray microanalyzer.Our preliminary findings indicate that the immersion marking method is effective for the Sr marking of fin rays in experimental fish.Moreover,we generated a bird’s-eye-view 3 D mesh image of the Sr concentrations,which can provide a more comprehensive information for fish stocking than that using normal 2 D imaging.
基金supported by the National Project of Scientific and Technical Supporting Programs (Grant Nos. 2006BAD09A01, 2006BAD09A06)the Hi-Tech Research and Development Program of China funded by Ministry of Science & Technology of China (Grant No. 2006AA10Z409)the National Natural Science Foundation of China (Grant No. 30871931)
文摘Optimal stocking densities were investigated for the sea cucumber Apostichopusjaponicus Selenka under feed-supplement and non-feed-supplement regimes in net enclosures for 333 d. Substantial weight loss occurred during the aestivation phase （AE）. Decreased growth rates were also observed during the winter phase （WT）. In contrast, sea cucumbers showed rapid growth during the spring （SP） and autumn （AU） phases. Feeding regimes considerably influenced the growtb performance, i.e., sea cucumbers grew faster under feed-supplement regime than under non-feed-supplement regime （P 〈 0.05）. The average survival rates of sea cucumbers under feed-supplement regime were higher than those under non-feed-supplement regime for both the autumn phase and spring phase, but the differences were only significant for the latter phase （P〈 0.05）. The fitted B-N curves showed that the optimal stocking densities, in terms of net production, were 22.3 ind. m^-2 for feed-supplement regime and 14.1 ind. m^-2 for non-feed-supplement regime.
基金Supported by the National Natural Science Foundation of China(No.31240012)the Earmarked Fund for Modern Agro-industry Technology Research System+2 种基金the Special Foundation for Postdoctoral Innovative Projects of Shandong Province(No.201101009)the National Key Technology R&D Program of China(No.2011BAD13B04)The Key Laboratory of Mariculture(KLM),Ministry of Education,Ocean University of China(OUC)
文摘Atlantic salmon Salmo salar were reared at four stocking densities--high density D1 （final density -39 kg/m^3）, medium densities DE （-29 kg/m^3） and D3 （~19 kg/m^3）, and low density D4 （-12 kg/m^3）- for 40 days to investigate the effect of stocking density on their growth performance, body composition and energy budgets. Stocking density did not significantly affect specific growth rate in terms of weight （SGRw） but did affect specific growth rate in terms of energy （SGRe）. Stocking density significantly influenced the ration level （RLw and RLe）, feed conversion ratio （FCRw and FCRe） and apparent digestibility rate （ADR）. Ration level and FCRw tended to increase with increasing density. Fish at the highest density D~ and lowest density D4 showed lower FCRe and higher ADR than at medium densities. Stocking density significantly affected protein and energy contents of the body but did not affect its moisture, lipid, or ash contents. The expenditure of energy for metabolism in the low-density and high-density groups was lower than that in the medium-density groups. Stocking density affected energy utilization from the feces but had no effect on excretion rate. The greater energy allocation to growth at high density and low density may be attributed to reduced metabolic rate and increased apparent digestibility rate. These findings provide information that will assist selection of suitable stocking densities in the Atlantic-salmon-farming industry.
基金Supported by the National Natural Science Foundation of China(No.30972267)the Special Fund for Agro-scientific Research in the Public Interest Project(No.201003024)the Knowledge Innovation Program of Chinese Academy of Sciences(No.KZCX2-EW-Q212)
文摘Limited information has been available about the influence of loading density on the performances of Scophthalmus maximus, especially in recirculating aquaculture systems （RAS）. In this study, turbot （13.84±2.74 g; average weigh±SD） were reared at four different initial densities （low 0.66, medium 1.26, sub-high 2.56, high 4.00 kg/m^2） for 10 weeks in RAS at 23±1℃ Final densities were 4.67, 7.25, 14.16, and 17.47 kg/m^2, respectively, which translate to 82, 108, 214, and 282 percent coverage of the tank bottom. Density had both negative and independent impacts on growth. The final mean weight, specific growth rate （SGR）, and voluntary feed intake significantly decreased and the coefficient of variation （CV） of final body weight increased with increase in stocking density. The medium and sub-high density groups did not differ significantly in SGR, mean weight, CV, food conversion rate （FCR）, feed intake, blood parameters, and digestive enzymes. The protease activities of the digestive tract at pH 7, 8.5, 9, and 10 were significantly higher for the highest density group, but tended to be lower （not significantly） at pH 4 and 8.5 for the lowest density group. The intensity of protease activity was inversely related to feed intake at the different densities. Catalase activity was higher （but not significantly） at the highest density, perhaps because high density started to induce an oxidative effect in turbot. In conclusion, turbot can be cultured in RAS at a density of less than 17.47 kg/m^2. With good water quality and no feed limitation, initial density between 1.26 and 2.56 kg/m^2 （final： 7.25 and 14.16 kg/m^2） would not negatively affect the turbot cultured in RAS. For culture at higher density, multi-level feeding devices are suggested to ease feeding competition.
基金supported by the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciences,and Trouw Nutrition Research&Development Centers.
文摘Background:High stocking density(HSD)stress has detrimental effects on growth performance,intestinal barrier function,and intestinal microbiota in intensive animal production.Organic acids(OA)are widely used as feed addi-tives for their ability to improve growth performance and intestinal health in poultry.However,whether dietary OA can ameliorate HSD stress-induced impaired intestinal barrier in broilers remains elusive.In this study,a total of 528 one-day-old male Arbor Acres broilers were randomly allocated into 3 treatments with 12 replicates per treatment including 10 birds for normal stocking density and 17 birds for HSD.The dietary treatments were as follows:1)Normal stocking density+basal diet;2)HSD+basal diets;3)HSD+OA.Results:HSD stress can induce increased levels of serum corticosterone,lipopolysaccharides,interleukin-1β,tumor necrosis factor-α,and down-regulated mRNA expression of ZO-1,resulting in compromised growth performance of broilers(P<0.05).Dietary OA could significantly reduce levels of serum corticosterone,lipopolysaccharides,interleukin-1β,and tumor necrosis factor-α,which were accompanied by up-regulated interleukin-10,mRNA expres-sion of ZO-1,and growth performance(P<0.05).Moreover,OA could down-regulate the mRNA expression of TLR4 and MyD88 to inhibit the NF-κB signaling pathway(P<0.05).Additionally,HSD stress significantly decreased the abundance of Bacteroidetes and disturbed the balance of microbial ecosystems,whereas OA significantly increased the abundance of Bacteroidetes and restored the disordered gut microbiota by reducing competitive and exploita-tive interactions in microbial communities(P<0.05).Meanwhile,OA significantly increased the content of acetic and butyric acids,which showed significant correlations with intestinal inflammation indicators(P<0.05).Conclusions:Dietary OA ameliorated intestinal inflammation and growth performance of broilers through restor-ing the disordered gut microbial compositions and interactions induced by HSD and elevating short-chain fatty acid production to inhibit the TLR4/NF-κB signaling pathway.These findings demonstrated the critical role of intestinal microbiota in mediating the HSD-induced inflammatory responses,contributing to exploring nutritional strategies to alleviate HSD-induced stress in animals.
基金supported by the System for Poultry Production Technology,Beijing Innovation Research Team of Modern Agriculture(CARSPSTP)the National Key Technology R&D Program of China during the 12~(th) five-year plan(2012BAD39B04)
文摘Background： Highly automated cage-rearing systems are becoming increasingly popular in China. However, a high stocking density can cause oxidative stress and decrease broiler performance. The tryptophan （TRP） derivative 5-hydroxytryptophan （5-HT） has been shown to preserve membrane fluidity in birds suffering from oxidative stress Therefore, this experiment was conducted to determine the effects of dietan/TRP supplementation on performance, breast meat quality and oxidative stress in broilers reared in cages with a high or low stocking density. Methods： Female Arbor Acres broilers （25-d-old, n = 144） were randomly allocated to 1 of 4 treatments. The birds were fed a diet based on corn, soybean meal, cottonseed meal and corn gluten meal containing either 0.18 or 0.27% TRP and were housed with stocking densities of 11 or 15.4 birds/m2 in a 2 x 2 factorial experiment. Broiler performance was evaluated from d 25 to 42. Eight birds from each treatment were slaughtered on d 42 and plasma and breast muscle samples were collected to measure biochemical indices. Results： A higher stocking density tended to be associated with reduced weight gain （P 〈 0.10）, and significantly increased plasma glutamic-pyruvic transaminase （GPT） activity （P 〈 0.001）. Increased dietary TRP significantly reduced the activities of lactic dehydrogenase and GPT while increasing total cholesterol in the plasma （P 〈 0.01）, reducing drip loss of breast muscle （P 〈 0.10） and improving feed efficiency （P 〈 0.10）. Conclusions： An increase in dietary TRP, ].S-fold higher than the standard supplementation level, can alleviate oxidative stress as well as improve welfare and feed efficiency in broilers reared in cages with a high stocking density.
基金Supported by the National Science Foundation for Young Scientists of China(No.31402314,31402283)the Agency of Science and Technology of Shandong Province(No.2013GHY11514)+4 种基金the Modern Agro-Industry Technology Research System(No.nycytx-50)the Scientific and Technology Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology(Nos.2015ASKJ02,2015ASKJ02-03-03)the Youth Innovation Promotion Association CAS,the Chinese Academy of Science and Technology Service Network Planning(No.KFJ-EW-STS-060)the China Agriculture Research System(No.CARS-47)the Shandong Provincial Key S&T Innovation Project(No.2017CXGC0101)
文摘Atlantic salmon reared in recirculating aquaculture system(RAS) may lead to inappropriately high stocking density, because ?sh live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the in?uence of stocking density on growth and some stress related physiological factors were investigated to evaluate the ef fects of stocking density. The ?sh were reared for 220 days at ?ve densities(A: 24 kg/m^3; B: 21 kg/m^3; C: 15 kg/m^3; D: 9 kg/m^3 and E: 6 kg/m^3). The results show that 30 kg/m^3 might be the maximum density which RAS can af ford in China. The stocking densities under 30 kg/m^3 have no ef fect on mortality of Atlantic salmon reared in RAS. However, the speci?c growth rate(SGR), ?nal weight and weight gain in the high density group were signi?cantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate(FCR) had a negative correlation with density. Plasma hormone T_3 and GH showed signi?cant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone(T 3), GH(growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT(glutamic oxalacetic transaminase) and GPT(glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no ef fects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise effcient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.
基金Supported by the National Special Research Fund for Non-Profit Sector(Agriculture)(No.201003055)
文摘The effects of stocking density on the growth and metabolism of Amur sturgeon were assessed. Amur sturgeon were grown for 70 days at three dif ferent stocking densities(low stocking density, LSD: 5.5 kg/m^3; medium stocking density, MSD: 8.0 kg/m^3; and high stocking density, HSD: 11.0 kg/m^3), and the biometric index, muscle composition, and serum biochemical parameters were evaluated. In addition, pituitary, liver, and muscle samples were collected for gene cloning and expression analyses. After 70 days of growth, the fish maintained at HSD had significantly lower fi nal body weight and specifi c growth rate, and a higher feed conversion ratio than those of the fish in the MSD and LSD groups. The HSD group had the lowest lipid and protein concentrations in serum and muscle. The serum cortisol concentration increased significantly in the HSD group, indicating that the stress-response system was activated in these fish. There was no change in the concentration of serum insulin-like growth factor 2(IGF-2), while the concentrations of serum growth hormone(GH) and insulin-like growth factor 1(IGF-1) decreased in the HSD group. The full-length cDNAs of G H and IGF-2 genes(995-bp and 1 207-bp long, respectively), were cloned and analyzed. In the HSD group, the expressions of GH in the pituitary and growth hormone receptor( GHR) and IGF-1 in the liver were down-regulated at the end of the 70-day experiment. In the HSD group, the transcript level of IGF-2 significantly decreased in the liver, but did not change in muscle. Overall, our results indicated that a HSD negatively af fects the growth performance and leads to changes in lipid and protein metabolism in Amur sturgeon. The down-regulated expression of genes related to the GH/IGF axis may be responsible for the poor growth performance of Amur sturgeon under crowding stress.
文摘Hybrid tilapia were reared at densities of 1, 5 or 10 fish per tank for four weeks. Mortality was 0 at 1 and 10 fish per tank, but was 25% at 5 fish per tank. Specific growth rate was highest at 1 fish per tank, and lowest at 5 fish per tank. The lower growth rate at the intermediate stocking density was associated with reduced feed efficiency, but there was no reduction in feed intake or digestibility. The results suggested increased metabolic cost caused by aggressive behaviour at intermediate stocking density, which can be suppressed by a further increase in density.
基金the National Natural Science Foundation of China(No.10771179)the Emphasis Subject of Guizhou Province of China
文摘We investigate a stage-structured delayed predator-prey model with impulsive stocking on prey and continuous harvesting on predator. According to the fact of biological resource management, we improve the assumption of a predator-prey model with stage structure for predator population that each individual predator has the same ability to capture prey. It is assumed that the immature and mature individuals of the predator population are divided by a fixed age, and immature predator population does not have the ability to attach prey. Sufficient conditions are obtained, which guarantee the global attractivity of predator-extinction periodic solution and the permanence of the system. Our results show that the behavior of impulsive stocking on prey plays an important role for the permanence of the system, and provide tactical basis for the biological resource management. Numerical analysis is presented to illuminate the dynamics of the system.
基金Supported by a grant from the National Scientific and Technological Supporting Program of China(No.2012BAD27B02-6)the Opening Foundation of the Jiangsu Engineering Laboratory for Characteristic Aquatic Species Breeding(No.CASB1306)the Knowledge Innovation Project of the Chinese Academy of Sciences(KSCX2-YW-N-47-06)
文摘The Chinese soft-shelled turtle Pelodiscus sinensis is a high-valued freshwater species cultured in China.This study investigated the effects of stocking density on water quality,growth performance and economic return of Pelodiscus sinensis cultured in ponds.P.sinensis were stocked at densities of 1 ind./m^2(LD:low stocking density) and 2 ind./m^2(HD:high stocking density) in 3 000-m^2 ponds,with three replicate ponds for each density.P.sinensis juveniles were fed with a commercial dry pellet feed of 46% crude protein and minced fillet of silver carp ans cultured for 122 days.The results showed that the levels of total nitrogen(TN),total phosphorous(TP),Chlorophyll-a(Chl.a) and turbidity in LD treatment were significantly lower than those in HD treatment(P<0.05).The mean TN and TP concentration in LD treatment was 29.3% and 35.7% lower compared to the HD treatment at the end of the experiment,respectively.Mean survival rates,final weight,average growth rates and PER were significantly higher in LD treatment compared with the HD treatment(P<0.05),respectively.Production was significantly affected by stocking density,which was higher in HD treatment,but the net income was higher in LD treatment.The results suggest that turtles with mean weight 55.6 g rearing at a low stocking density(1 ind./m^2) in ponds had a positive effect on overall economic return and was effective at improving turtle growth performance and water quality.
基金Supported by Chinese National Key Research and Development Program(2016YFD0500510).
文摘Stocking density is an important factor for animal production performance.It appears particularly urgent and important to research systematically on stocking density for the dual restriction of environmental protection and animal welfare.This review dealt with the influence of stocking density on ducks'productivity and health through production performance,animal behavior and animal welfare,and analyzed the possible mechanism of high stocking density reducing production performance,resulting in abnormal behavior and stress,causing welfare and health problems.We consider that it might be associated with heat stress,more spatial competition(to gather the food,drinking water,sports area,etc.),as well as the quality deterioration of litter and house air caused by high stocking density.Finally,we put forward some suggestions on the study of stocking density's effects on ducks,and proposed the future work that need to be studied and problems that need to be solved imminently.
基金The National Natural Science Foundation of China under contract Nos 31402315 and 31240012the Modern Agriculture Industry System Construction of Special Funds under contract No.CARS-50-G10+1 种基金the Key R&D Program of Jiangsu Province under contract No.BE2015328a foundation from the Key Laboratory of Mariculture&Stock Enhancement in North China’s Sea,Ministry of Agriculture,China
文摘Stocking density is widely recognized as a critical factor in aquaculture and a potential source of long-term stress.The influence of stocking density on growth and stress response of juvenile turbot（Scophthalmus maximus, ~3–75g, initial to final weight） was examined in fish held under low（LD, ~0.21–5.31 kg/m^2, initial to final density）,medium（MD, ~0.42–10.81 kg/m^2） and high stocking density（HD, ~0.63–14.27 kg/m^2） for 120 days in a recirculating aquaculture system（RAS）. In this trial, the growth curve for weight of juvenile turbot in RAS, all fitted by the Schnute model. No significant difference was found in growth performance among the three densities until at the final sampling（Day 120）. The final weight and body weight increase（BWI） in the HD group were significantly lower than in other groups（P〈0.05, weight：（75.83±2.49） g,（75.39±2.08） g,（65.72±2.86） g and BWI：（2 436.12±28.10）%,（2 421.29±4.64）%,（2 097.88±20.99）% in LD, MD and HD groups, respectively）. Similarly, the specific growth rate（SGR）, feed conversion ratio（FCR） and coefficient of variation for weight（CV_w） were adversely affected by high stocking density（P〈0.05）. However, there was no difference in survival and Fulton＇s condition factor（K） of turbot among the different groups. Physiological analyses demonstrated a clear increase in the plasma cortisol level and an obvious decrease in growth hormone（GH） concentration in the HD group on Day120（P〈0.05）. There was no significant effect of stocking density on plasma glucose, Cl– and protein levels. All these findings would provide a reference for selecting the optimal stocking density of juvenile turbot in RAS.
文摘The inadequate supply of tilapia fingerling is a major limitation to cage culture development in Ghana. Lake-based hapa systems are very efficient in nursing fingerlings although the process can be tricky due to inherent effects of stocking densities and environmental factors. This study aimed at assessing the growth, survival and profitability of Nile tilapia (Oreochromis niloticus) fingerlings of the Akosombo strain reared in nine 1 m<sup>3</sup> Lake-based hapas at different densities. Each hapa was stocked with 2.12 ± 0.14 g sex-reversed tilapia fingerlings at varying stocking densities of 400 fish/m<sup>3</sup>, 800 fish/m<sup>3</sup> and 1200 fish/m<sup>3</sup> serving as treatments T1, T2 and T3 respectively. Each treatment was replicated thrice. Bi-weekly samplings were done and water quality parameters were measured. After the experiment, analysis of variance showed significant differences (p
基金supported by the National Natural Science Foundation of China (Nos. 31972789 and 31772843)the Industrial Development Project of Qingdao City (No. 20-3-4-16-nsh)the Science and Technology Development Project of Weihai City (No. 2018NS01)
文摘The Pacific oyster Crassostrea gigas is one of the most widely cultivated aquaculture species and contributes signifi-cantly to total seafood production for human beings.However,mass mortality occurred frequently,and in some regions almost all oysters died during seed production and grow-out stage.In order to explore whether hybridization breeding can improve its growth and survival,a complete diallel cross between a selected strain‘Haida No.1’(S)and an orange shell variant(O)of C.gigas was carried out.The larval growth and survival were compared among hybrids and purebred strains at temperatures of 16,20,24,28 and 32℃;salinities of 15,20,25,30 and 35;and stocking densities of 0.5,1,2,4 and 8 larvae mL−1.Under different environments,the hybridization between two strains of C.gigas showed the heterosis of growth and survival.The mean shell height and survival rate of the two reciprocal crosses(OS,SO)were significantly higher than those of the two purebred strains(SS,OO)under all environ-mental conditions.In particular,OS showed greater heterosis than the purebred strains and SO progeny.The results showed that the productive traits of the‘Haida No.1’could be improved by crossing with the orange shell line.Meanwhile,the results from this study also indicated that hybridization between the two strains of C.gigas may be a promising way for breeding new variety with high survival rate.
基金Supported by the Modern Agro-Industry Technology Research System(No.CARS-48)the National Natural Science Foundation of China(No.31240012)+1 种基金the Special Foundation for Postdoctoral Innovative Projects of Shandong Province(No.201101009)the National Key Technology R&D Program of China(No.2011BAD13B04)
文摘The combined effects of feeding rate(0.8%,1.0%,and 1.2%initial body weight/day),feeding frequency(two,three,and four times/day) and stocking density(10,15,and 20 kg/m3) in recirculating aquaculture systems(RAS) on growth performance,digestion and waste generation of Atlantic salmon(Salmo salar L.) were investigated in an 8-week orthogonal experiment(L9(3)3) with a constant daily water renewal at 7.50%of total volume.No mortality occurred during the experimental period.Feed conversion ratio(FCR) varied from 0.90 to 1.13 and specific growth rate(SGR) ranged from 0.48%to 0.69%/day.SGR,thermal growth coefficient(TGC) and FCR were not significantly(P>0.05) affected by the three factors,while net protein utilization(NPU) was significantly(P<0.05) affected.Apparent digestibility coefficients(ADC) of dry matter in the present study were in the range 66.12%-73.55%.ADC in protein,lipid and energy were statistically different among all treatments and in the range of 90.07%-93.67%,81.54%-89.15%,and67.55%-71.87%,respectively.The proportion of mean total ammonia nitrogen excreted ranged from 1.37%to 1.64%of feed nitrogen at steady state,and the concentration of nitrogenous and phosphorus compounds were differently correlated to the three factors.The results will provide valuable reference data for culture management decisions in the Atlantic salmon farming industry.
基金Supported by the Key Technology Research Program of Ministry of Agriculture (No. 04-11-01B) and the National High-tech Research and Development Program of China (863 Program) (No. 2004AA603320 and 2004AA603330)
文摘Juvenile turbot （Scophthalmus maximus） the initial stock densities at 0.28, 0.87, 1.12, 1.16, 2.75 were reared in five different experiment groups in kg/m^2 respectively for the study of the density effect on growth, pigmentation and feed coefficient rate. The experiment lasted for 60 days with final stock densities at 1.91, 6.31, 8.86, 11.97, 17.67 kg/m^2 respectively. Result showed that, in the same experiment condition, the stocking density has a positive effect on growth in low density and negative in high density. The SGR （special growth rate） was 3.189, 3.304, 3.447, 3.341, and 3.087 respectively. The uniformity of weight distributing among experiment groups decreased with increasing density. The stocking density had positive effect on feed coefficient rate. Group 1 had the least feed coefficient rate 0.96, and highest at 1.25 in Group 5, the highest density group. High stocking density inhibited the growth and increased the feed coefficient rate. The stocking density had negative relationship to pigmentation improvement for whitened fish.
基金The work was supported by the NZ Ministry of Foreign Aff air and Trade(MFAT)’s NZAID Programme.
文摘Silvicultural practices applied in managed forest plantations may help counteract the effects of climate change by influencing soil surface CO_(2)efflux(Fs).Understanding the effects of silvicultural practices on Fs will provide unbiased estimates of carbon fluxes and allow better silvicultural decisions for carbon sequestration.Therefore,we assessed how Fs differed seasonally across silvicultural practices(i.e.,stocking levels,clone,fertilization and weed control treatments)and evaluated the effects of soil temperature(Ts)and soil volumetric water content(θv)on Fs across these practices for a mid-rotation(14 year-old)Pinus radiata plantation in the Canterbury region of New Zealand.There were significant differences in Fs(p<0.05)over the four seasons,three levels of stocking,and five clones.The effects of fertilization and weed control applied 12 years previously on Fs were insignificant.Annual estimate of Fs(mean±1 standard deviation)from the study site was 22.7±7.1 t ha^(-1)a^(-1)in the form of CO_(2)(6.2±2.1 t ha^(-1)a^(-1)in the form of C).Fs values were consistently higher in plots with 1250 stems ha^(-1)compared to 2500 stems ha^(-1),which may be related to a strong soil resource limitation because of the close spacing in the latter plantation.Significant differences in Fs across clones suggest that variations in carbon partitioning might explain their growth performance.Silvicultural treatments influenced Fs response to soil temperature(p<0.05),resulting in models explaining 28-49%of the total variance in Fs.These findings provide insights into how silvicultural management decisions may impact Fs in mid-rotation radiata pine plantations,contributing towards developing more precise and unbiased plantation carbon budgets.
