The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were ...The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were the optimum for breaking dormancy of seeds due to their simple and easy operation in techniques, economic and rapid effectiveness in performance and high germination rates (77.6% and 88.0% respectively).展开更多
[Objective] Taking silkworm （Bombyx mori） as the test material, the research aimed to study the toxicity effects of Euphorbiafischeriana Steud. against the insects in Lepidoptera. [ Methods ] Water extract was prepa...[Objective] Taking silkworm （Bombyx mori） as the test material, the research aimed to study the toxicity effects of Euphorbiafischeriana Steud. against the insects in Lepidoptera. [ Methods ] Water extract was prepared with fresh perennial E. fischer/ana Steud. , which was applied in silkworm with different methods including daubing the extract on silkworm back, soaking silkworm in the extract and feeding silkworm with the mulberry leaves daubed by the extract. [ Re- suits] Those silkworms whose backs were daubed with the water extract had no obvious poisoning symptom. The silkworms soaked for 1 and 3 min could be survived for about 7 d; the silkworms soaked for 5 min could be survived for about 2 d; and the silkworms soaked for 15 rain could be survived for 10 h at most. Those silk- worms fed with mulberry leaves daubed by water extract completely died after 2 d. [ Conduslons ] The water extract of E. fischer/ana Steud. had significant contact toxicity and stomach poisoning against silkworm.展开更多
It is very important to find out the reasons and the morphological changes of cattle abortion, death embryo and teratism in Sanjiang area, in order to determine the preventive measures, to improve animal quality, and ...It is very important to find out the reasons and the morphological changes of cattle abortion, death embryo and teratism in Sanjiang area, in order to determine the preventive measures, to improve animal quality, and to accelerate the animal industry. In the present studies, 25 cows and 25 local bos calves were investigated. The powder of Veratrum nigrum L. and Euphorbia fischeriana steud. was medicated to the animals during the 15 - 19th day of gestation. It was found that there were different poisoning reactions. When the poisoning was on the 15 - 16th day of gestation, the pregnant animals were easy to miscarriage. When the poisoning was on the 17 - 18th day of gestation, the embryos were easy to become teratism. The joint malformation bicephalus and rachischisis could take place for calves. If the poisoning was after 19th day of gestation , there were much more death embryos. The results of the studies showed that Veratrum and Euphorbia fischeriana steud. were the most poisonous plants to the animal industry of Sanjiang area. Some preventive measures were proposed.展开更多
High concentrations of heavy metals (Pb, Zn, As, Cd) were found in the vegetative and generative organs of Paulownia tomentosa (Thunb.) Steud., 1841) in industrial production zones and along transport routes in Samark...High concentrations of heavy metals (Pb, Zn, As, Cd) were found in the vegetative and generative organs of Paulownia tomentosa (Thunb.) Steud., 1841) in industrial production zones and along transport routes in Samarkand, Samarkand region of the Republic of Uzbekistan. The obtained results confirm the potential of using this plant for phytoremediation purposes.展开更多
[Objectives]To determine the optimal preparation technology of Clerodendrum bungei Steud.extract gel by orthogonal test and gel quality test method in General Rule 0114 of Chinese Pharmacopoeia(Volume IV,2020 Edition)...[Objectives]To determine the optimal preparation technology of Clerodendrum bungei Steud.extract gel by orthogonal test and gel quality test method in General Rule 0114 of Chinese Pharmacopoeia(Volume IV,2020 Edition),and to study its anorectal pharmacodynamics and drug release in vitro.[Methods]Carbomer 940,propylene glycol and absolute ethyl alcohol were selected as the main factors,and the preparation technology of C.bungei Steud.extract gel was optimized by orthogonal test.The mouse model of ulcerative hemorrhoids was established with glacial acetic acid(HAC)and compared with Ma Yinglong musk hemorrhoids ointment.The recovery of trauma was compared between the two groups.At the same time,porcine small intestine was used as semi-permeable membrane to make diffusion cell to simulate anal environment,and the drug release in vitro was studied.[Results]The C.bungei Steud.extract gel was smooth in appearance and good in stability.It could effectively treat anal ulcer in mice and release quickly in vitro.[Conclusions]The formula is reasonable,and the effect of animal experiment is remarkable,which can provide a new treatment plan for ulcerative hemorrhoids.展开更多
The active components,targets,and pathways of Euphorbia fischeriana Steud.-Jujubae Fructus in treating hepatocirrhosis and the mechanism of action were explored by means of network pharmacology.Firstly,the active comp...The active components,targets,and pathways of Euphorbia fischeriana Steud.-Jujubae Fructus in treating hepatocirrhosis and the mechanism of action were explored by means of network pharmacology.Firstly,the active components and related targets of Jujubae Fructus were screened by TCMSP database and standardized by Uniprot database.The compounds of Euphorbia fischeriana Steud.were obtained by searching the literature and finally screened by PubChem database,Swiss ADME,and SwissTargetPrediction.Hepatocirrhosis targets were obtained through Genecards database,PPI network analysis was conducted on common targets of Euphorbia fischeriana Steud.-Jujubae Fructus and hepatocirrhosis by using String database,GO enrichment analysis and KEGG pathway enrichment analysis was conducted through Metascape database by using intersection targets of Euphorbia fischeriana Steud.-Jujubae Fructus and hepatocirrhosis,and the results were drawn by using Weishengxin online drawing platform.Then,the network of drug-compound-target-pathway was constructed by the software of Cytoscape3.8.0.Finally,the above results were verified by molecular docking.47 active compounds from Euphorbia fischeriana Steud.-Jujubae Fructus were screened out,which had 38 common targets,162 intersection targets,and 340 signal pathways with hepatocirrhosis,mainly involving hepatitis C,JAK-STAT signal pathway and AGE-RAGE signal pathway.Targets,such as MAPK1,AKT1,TNF,JUN,IL6 and PTGS2,play important roles in the treatment.The findings suggested that the main active ingredients of Euphorbia fischeriana Steud.-Jujubae Fructus in treating hepatocirrhosis are quercetin,scopolamine,physcion,7-deoxyrangduin,17-Hydroxyjolkinolide A,etc.Molecular docking results showed that the main active components and core targets might have a good binding capacity.This study preliminarily explored the potential mechanism of Euphorbia fischeriana Steud.-Jujubae Fructus in treating hepatocirrhosis and provided a theoretical basis for the clinical application of Euphorbia fischeriana Steud.-Jujubae Fructus.展开更多
目的 系统梳理2000—2025年大戟科大戟属3种药用植物(京大戟、狼毒大戟、月腺大戟)的研究热点与趋势,为后续研究提供方向参考。方法 以中国学术期刊全文数据库(CNKI)、Web of Science(WOS)为数据源,借助Excel整理文献,运用VOSviewer、Ci...目的 系统梳理2000—2025年大戟科大戟属3种药用植物(京大戟、狼毒大戟、月腺大戟)的研究热点与趋势,为后续研究提供方向参考。方法 以中国学术期刊全文数据库(CNKI)、Web of Science(WOS)为数据源,借助Excel整理文献,运用VOSviewer、Citespace软件对发文量、研究机构、核心作者及关键词等维度进行计量分析与可视化呈现。结果 文献收录情况:京大戟纳入中文文献83篇、英文文献65篇,中文文献2007年后呈波动上升趋势,英文文献则表现为间歇性活跃;狼毒大戟纳入中文文献213篇、英文文献63篇,中文文献2012年达发文峰值(19篇),英文文献2012年后趋于稳定;月腺大戟纳入中文文献78篇、英文文献28篇,中文文献2012年达峰值(11篇)后逐步回落,英文文献2018年达高峰(8篇)。研究主体特征:京大戟研究中,南京中医药大学中、英文发文量均居首位,中文核心作者为曹雨诞(12篇),英文领域以Kim Jin Sook发文最多(7篇);狼毒大戟研究中,齐齐哈尔医学院中文发文26篇居首,中、英文文献的核心作者均为刘吉成(中文12篇、英文7篇);月腺大戟研究中,南京中医药大学中文发文11篇领先,英文领域以大连医科大学为核心形成合作网络,中文核心作者为严小红(6篇),英文领域Wang Chao等3人发文均≥5篇。研究热点差异:京大戟中文核心关键词聚焦炮制工艺、化学成分解析及毒性机制,英文文献侧重化学成分与药理作用机制;狼毒大戟中文研究聚焦炮制对成分及药理活性的影响,英文文献侧重成分挖掘与药理分子机制;月腺大戟中文研究关注其与同属“狼毒”基原植物的成分鉴定及炮制影响,英文文献同样以成分与分子机制为核心。3种植物虽具分类与药用共性,核心研究方向均集中于成分解析、活性评价、毒性探究及炮制优化;成分上有共有类别但结构特异,药理均具抗肿瘤等潜力但活性成分不同,毒性均表现为肠道、肝肾及细胞毒性,炮制减毒方法多样但技术标准化与量化关系仍待完善。结论 揭示3种大戟属药用植物的研究热点、发展现状及核心差异,明确炮制与成分、活性及毒性的关联为该领域核心研究方向,为后续深化物质基础解析、完善构效关系及推动炮制技术标准化提供思路。展开更多
In order to determine genetic diversity of Oryza meyeriana (Zoll. et Mor. ex Steud. ) Baill., 12 enzyme systems encoded by 17 loci were electrophoretically analyzed in 164 individuals of seven populations from Simao P...In order to determine genetic diversity of Oryza meyeriana (Zoll. et Mor. ex Steud. ) Baill., 12 enzyme systems encoded by 17 loci were electrophoretically analyzed in 164 individuals of seven populations from Simao Prefecture, Yunnan Province, China. In comparison with those seed plants with the same life history and breeding systems, as well as the other species in the genus Oryza, the species shows rather low levels of genetic diversity (A = 1.1, P = 8.0%, Ho = 0.004 and He = 0. 015) within populations and high genetic differentiation among populations. FST was up to 0. 649, suggesting that 64. 9% of total genetic variability exists among populations. Considering high genetic differentiation among populations from a limited geographic region, most of the populations of the species are worth being protected, and therefore, great natural protection regions should theoretically be established in which a great deal of populations should be involved for developing in situ conservation management. Meanwhile, some priory localities for in situ conservation of O. meyeriana in Yunnan Province, were proposed.展开更多
Objective:To investigate the anti-liver cancer effects and aspartic acid(Asp)-related action mechanism of Euphorbia fischeriana Steud.(LD).Methods:The mice model of liver cancer was established by injection of H22 cel...Objective:To investigate the anti-liver cancer effects and aspartic acid(Asp)-related action mechanism of Euphorbia fischeriana Steud.(LD).Methods:The mice model of liver cancer was established by injection of H22 cells.After 5 days,mice were randomly divided into model group,sorafenib group(20 mg/kg),LD high-dose(LDH,1.36 g/kg) group,LD medium-dose(LDM,0.68 g/kg) group,and LD low-dose(LDL,0.34 g/kg)group,10 mice each group.Drugs were intragastrically administered to the mice once daily for 10 days,respectively.Body weight,tumor size and tumor weight were recorded.Hepatic index was calculated.Pathological changes of liver cancer tissues were evaluated by hematoxylin and eosin staining and TUNEL staining.Liquid chromatography-mass spectrometer was used to analyze different metabolites between the model and LDH groups.Results:After LD treatment,tumor weight,tumor size and hepatic index were reduced compared with the model group.Necrocytosis and karyorrhexis of tumor cells were found.Moreover,61 differential metabolites(18 up-regulated,43 down-regulated) were affirmed and 20 pathways of KEGG(P<0.05) were gotten.In addition,Bel-7402,HepG2 and H22 cell viabilities were significantly increased after adding Asp into the medium.And then,the cell proliferation effect induced by Asp was ameliorated by LD.Conclusion:The anti-liver cancer efficacy of LD extract was validated in H22 mice model,and inhibition of Asp level might be the underlying mechanism.展开更多
Objective To identify the compounds withα-glucosidase inhibitory activity from Clerodendrum bungei Steud(Chou Mu Dan,臭牡丹)using HPLC-ESI-QTOF-MS/MS.Methods The ethanol extracts of Clerodendrum bungei Steud(Chou Mu ...Objective To identify the compounds withα-glucosidase inhibitory activity from Clerodendrum bungei Steud(Chou Mu Dan,臭牡丹)using HPLC-ESI-QTOF-MS/MS.Methods The ethanol extracts of Clerodendrum bungei Steud(Chou Mu Dan,臭牡丹)were partitioned with petroleum ether,ethyl acetate,n-butanol,and water.The assay forα-glucosidase inhibitory activity revealed strongα-glucosidase inhibitory activity in the ethyl acetate fraction,and the bioactive compounds present in this fraction were identified by the HPLCESI-QTOF-MS/MS method.Results A total of 29 compounds were determined,among the identified bioactive components;these included 12 phenylethanoid glycosides(compounds 5,6,17,20-22,24),7 flavonoids(compounds 10,19,23,25-28),5 phenolic acids(compounds 2-4,7,9),and 5 other compounds.Compounds 2-4,7,9-10,12-13,15,19,and 26,with a potentialα-glucosidase inhibitory activity,have been reported previously.Conclusions Our results show that the methodology used in this study is feasible,credible,and rapid in identifying known compounds and also for characterizing new natural glucosidase inhibitory candidates from Clerodendrum bungei Steud(Chou Mu Dan,臭牡丹).展开更多
文摘The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were the optimum for breaking dormancy of seeds due to their simple and easy operation in techniques, economic and rapid effectiveness in performance and high germination rates (77.6% and 88.0% respectively).
基金Supported by Research Project Plan in Chengde Medical College(200925)~~
文摘[Objective] Taking silkworm （Bombyx mori） as the test material, the research aimed to study the toxicity effects of Euphorbiafischeriana Steud. against the insects in Lepidoptera. [ Methods ] Water extract was prepared with fresh perennial E. fischer/ana Steud. , which was applied in silkworm with different methods including daubing the extract on silkworm back, soaking silkworm in the extract and feeding silkworm with the mulberry leaves daubed by the extract. [ Re- suits] Those silkworms whose backs were daubed with the water extract had no obvious poisoning symptom. The silkworms soaked for 1 and 3 min could be survived for about 7 d; the silkworms soaked for 5 min could be survived for about 2 d; and the silkworms soaked for 15 rain could be survived for 10 h at most. Those silk- worms fed with mulberry leaves daubed by water extract completely died after 2 d. [ Conduslons ] The water extract of E. fischer/ana Steud. had significant contact toxicity and stomach poisoning against silkworm.
文摘It is very important to find out the reasons and the morphological changes of cattle abortion, death embryo and teratism in Sanjiang area, in order to determine the preventive measures, to improve animal quality, and to accelerate the animal industry. In the present studies, 25 cows and 25 local bos calves were investigated. The powder of Veratrum nigrum L. and Euphorbia fischeriana steud. was medicated to the animals during the 15 - 19th day of gestation. It was found that there were different poisoning reactions. When the poisoning was on the 15 - 16th day of gestation, the pregnant animals were easy to miscarriage. When the poisoning was on the 17 - 18th day of gestation, the embryos were easy to become teratism. The joint malformation bicephalus and rachischisis could take place for calves. If the poisoning was after 19th day of gestation , there were much more death embryos. The results of the studies showed that Veratrum and Euphorbia fischeriana steud. were the most poisonous plants to the animal industry of Sanjiang area. Some preventive measures were proposed.
文摘High concentrations of heavy metals (Pb, Zn, As, Cd) were found in the vegetative and generative organs of Paulownia tomentosa (Thunb.) Steud., 1841) in industrial production zones and along transport routes in Samarkand, Samarkand region of the Republic of Uzbekistan. The obtained results confirm the potential of using this plant for phytoremediation purposes.
基金Supported by National Natural Science Foundation of China(31671954)。
文摘[Objectives]To determine the optimal preparation technology of Clerodendrum bungei Steud.extract gel by orthogonal test and gel quality test method in General Rule 0114 of Chinese Pharmacopoeia(Volume IV,2020 Edition),and to study its anorectal pharmacodynamics and drug release in vitro.[Methods]Carbomer 940,propylene glycol and absolute ethyl alcohol were selected as the main factors,and the preparation technology of C.bungei Steud.extract gel was optimized by orthogonal test.The mouse model of ulcerative hemorrhoids was established with glacial acetic acid(HAC)and compared with Ma Yinglong musk hemorrhoids ointment.The recovery of trauma was compared between the two groups.At the same time,porcine small intestine was used as semi-permeable membrane to make diffusion cell to simulate anal environment,and the drug release in vitro was studied.[Results]The C.bungei Steud.extract gel was smooth in appearance and good in stability.It could effectively treat anal ulcer in mice and release quickly in vitro.[Conclusions]The formula is reasonable,and the effect of animal experiment is remarkable,which can provide a new treatment plan for ulcerative hemorrhoids.
基金supported by Qiqihar Science and Technology Plan Joint Guidance Project (LSFGG-2022042).
文摘The active components,targets,and pathways of Euphorbia fischeriana Steud.-Jujubae Fructus in treating hepatocirrhosis and the mechanism of action were explored by means of network pharmacology.Firstly,the active components and related targets of Jujubae Fructus were screened by TCMSP database and standardized by Uniprot database.The compounds of Euphorbia fischeriana Steud.were obtained by searching the literature and finally screened by PubChem database,Swiss ADME,and SwissTargetPrediction.Hepatocirrhosis targets were obtained through Genecards database,PPI network analysis was conducted on common targets of Euphorbia fischeriana Steud.-Jujubae Fructus and hepatocirrhosis by using String database,GO enrichment analysis and KEGG pathway enrichment analysis was conducted through Metascape database by using intersection targets of Euphorbia fischeriana Steud.-Jujubae Fructus and hepatocirrhosis,and the results were drawn by using Weishengxin online drawing platform.Then,the network of drug-compound-target-pathway was constructed by the software of Cytoscape3.8.0.Finally,the above results were verified by molecular docking.47 active compounds from Euphorbia fischeriana Steud.-Jujubae Fructus were screened out,which had 38 common targets,162 intersection targets,and 340 signal pathways with hepatocirrhosis,mainly involving hepatitis C,JAK-STAT signal pathway and AGE-RAGE signal pathway.Targets,such as MAPK1,AKT1,TNF,JUN,IL6 and PTGS2,play important roles in the treatment.The findings suggested that the main active ingredients of Euphorbia fischeriana Steud.-Jujubae Fructus in treating hepatocirrhosis are quercetin,scopolamine,physcion,7-deoxyrangduin,17-Hydroxyjolkinolide A,etc.Molecular docking results showed that the main active components and core targets might have a good binding capacity.This study preliminarily explored the potential mechanism of Euphorbia fischeriana Steud.-Jujubae Fructus in treating hepatocirrhosis and provided a theoretical basis for the clinical application of Euphorbia fischeriana Steud.-Jujubae Fructus.
文摘目的 系统梳理2000—2025年大戟科大戟属3种药用植物(京大戟、狼毒大戟、月腺大戟)的研究热点与趋势,为后续研究提供方向参考。方法 以中国学术期刊全文数据库(CNKI)、Web of Science(WOS)为数据源,借助Excel整理文献,运用VOSviewer、Citespace软件对发文量、研究机构、核心作者及关键词等维度进行计量分析与可视化呈现。结果 文献收录情况:京大戟纳入中文文献83篇、英文文献65篇,中文文献2007年后呈波动上升趋势,英文文献则表现为间歇性活跃;狼毒大戟纳入中文文献213篇、英文文献63篇,中文文献2012年达发文峰值(19篇),英文文献2012年后趋于稳定;月腺大戟纳入中文文献78篇、英文文献28篇,中文文献2012年达峰值(11篇)后逐步回落,英文文献2018年达高峰(8篇)。研究主体特征:京大戟研究中,南京中医药大学中、英文发文量均居首位,中文核心作者为曹雨诞(12篇),英文领域以Kim Jin Sook发文最多(7篇);狼毒大戟研究中,齐齐哈尔医学院中文发文26篇居首,中、英文文献的核心作者均为刘吉成(中文12篇、英文7篇);月腺大戟研究中,南京中医药大学中文发文11篇领先,英文领域以大连医科大学为核心形成合作网络,中文核心作者为严小红(6篇),英文领域Wang Chao等3人发文均≥5篇。研究热点差异:京大戟中文核心关键词聚焦炮制工艺、化学成分解析及毒性机制,英文文献侧重化学成分与药理作用机制;狼毒大戟中文研究聚焦炮制对成分及药理活性的影响,英文文献侧重成分挖掘与药理分子机制;月腺大戟中文研究关注其与同属“狼毒”基原植物的成分鉴定及炮制影响,英文文献同样以成分与分子机制为核心。3种植物虽具分类与药用共性,核心研究方向均集中于成分解析、活性评价、毒性探究及炮制优化;成分上有共有类别但结构特异,药理均具抗肿瘤等潜力但活性成分不同,毒性均表现为肠道、肝肾及细胞毒性,炮制减毒方法多样但技术标准化与量化关系仍待完善。结论 揭示3种大戟属药用植物的研究热点、发展现状及核心差异,明确炮制与成分、活性及毒性的关联为该领域核心研究方向,为后续深化物质基础解析、完善构效关系及推动炮制技术标准化提供思路。
基金Project supported by the Grant of the President of the Chinese Academy of Sciences.
文摘In order to determine genetic diversity of Oryza meyeriana (Zoll. et Mor. ex Steud. ) Baill., 12 enzyme systems encoded by 17 loci were electrophoretically analyzed in 164 individuals of seven populations from Simao Prefecture, Yunnan Province, China. In comparison with those seed plants with the same life history and breeding systems, as well as the other species in the genus Oryza, the species shows rather low levels of genetic diversity (A = 1.1, P = 8.0%, Ho = 0.004 and He = 0. 015) within populations and high genetic differentiation among populations. FST was up to 0. 649, suggesting that 64. 9% of total genetic variability exists among populations. Considering high genetic differentiation among populations from a limited geographic region, most of the populations of the species are worth being protected, and therefore, great natural protection regions should theoretically be established in which a great deal of populations should be involved for developing in situ conservation management. Meanwhile, some priory localities for in situ conservation of O. meyeriana in Yunnan Province, were proposed.
基金Supported by National Natural Science Foundation of China(No.81873249)National Natural Science Foundation of Shandong Province(No.ZR2022MH319)Young Taishan Scholars Program of Shandong Province(No.tsqn201909200)。
文摘Objective:To investigate the anti-liver cancer effects and aspartic acid(Asp)-related action mechanism of Euphorbia fischeriana Steud.(LD).Methods:The mice model of liver cancer was established by injection of H22 cells.After 5 days,mice were randomly divided into model group,sorafenib group(20 mg/kg),LD high-dose(LDH,1.36 g/kg) group,LD medium-dose(LDM,0.68 g/kg) group,and LD low-dose(LDL,0.34 g/kg)group,10 mice each group.Drugs were intragastrically administered to the mice once daily for 10 days,respectively.Body weight,tumor size and tumor weight were recorded.Hepatic index was calculated.Pathological changes of liver cancer tissues were evaluated by hematoxylin and eosin staining and TUNEL staining.Liquid chromatography-mass spectrometer was used to analyze different metabolites between the model and LDH groups.Results:After LD treatment,tumor weight,tumor size and hepatic index were reduced compared with the model group.Necrocytosis and karyorrhexis of tumor cells were found.Moreover,61 differential metabolites(18 up-regulated,43 down-regulated) were affirmed and 20 pathways of KEGG(P<0.05) were gotten.In addition,Bel-7402,HepG2 and H22 cell viabilities were significantly increased after adding Asp into the medium.And then,the cell proliferation effect induced by Asp was ameliorated by LD.Conclusion:The anti-liver cancer efficacy of LD extract was validated in H22 mice model,and inhibition of Asp level might be the underlying mechanism.
基金the funding support from the China National Natural Science Foundation Funding Project(NO.81503452)Natural Science Foundation of Hunan Province,China(No.14JJ4066)
文摘Objective To identify the compounds withα-glucosidase inhibitory activity from Clerodendrum bungei Steud(Chou Mu Dan,臭牡丹)using HPLC-ESI-QTOF-MS/MS.Methods The ethanol extracts of Clerodendrum bungei Steud(Chou Mu Dan,臭牡丹)were partitioned with petroleum ether,ethyl acetate,n-butanol,and water.The assay forα-glucosidase inhibitory activity revealed strongα-glucosidase inhibitory activity in the ethyl acetate fraction,and the bioactive compounds present in this fraction were identified by the HPLCESI-QTOF-MS/MS method.Results A total of 29 compounds were determined,among the identified bioactive components;these included 12 phenylethanoid glycosides(compounds 5,6,17,20-22,24),7 flavonoids(compounds 10,19,23,25-28),5 phenolic acids(compounds 2-4,7,9),and 5 other compounds.Compounds 2-4,7,9-10,12-13,15,19,and 26,with a potentialα-glucosidase inhibitory activity,have been reported previously.Conclusions Our results show that the methodology used in this study is feasible,credible,and rapid in identifying known compounds and also for characterizing new natural glucosidase inhibitory candidates from Clerodendrum bungei Steud(Chou Mu Dan,臭牡丹).
