The objective of the present investigation was to evaluate the<i><span style="font-family:Verdana;"> Urochloa</span></i><span style="font-family:Verdana;"> Insurgent, ...The objective of the present investigation was to evaluate the<i><span style="font-family:Verdana;"> Urochloa</span></i><span style="font-family:Verdana;"> Insurgent, Piata and Signal grasses by varying the phenology in the attributes: stem population dynamics, tissue replacement, leaf:stem relation and weight per stem. The data were analyzed using a completely randomized block design with arrangement in divided plots and four replications, the procedure used was PROC GLM from SAS. The Signal grass presented higher stem density with an average of 450 m</span><sup><span style="font-family:Verdana;">?2</span></sup><span style="font-family:Verdana;"> stems, while the meadow with Insurgent grass registered the lowest stem density throughout the investigation, with an average of 320 m</span><sup><span style="font-family:Verdana;">-2</span></sup><span style="font-family:Verdana;"> stems;furthermore, in this treatment, the stem density tended to increase slowly over time (P = 0.05). The Insurgente and signal grasses showed rapid leaf elongation from day 21 of regrowth with 123 and 104 cm stem</span><sup><span style="font-family:Verdana;">?1</span></sup><span style="font-family:Verdana;">, while in Piata the accelerated leaf elongation was on day 14 with 113 cm stem</span><sup><span style="font-family:Verdana;">?1</span></sup><span style="font-family:Verdana;"> where it remained active its growth until day 56 (P > 0.05). As the regrowth age was increasing, the population dynamics of stems and weight per stem were increasing;in Insurgent and signal the leaf elongation and net growth increased to a maximum point to start declining;however, in Piata grass was increasing without decreasing. The trend of the leaf:stem relation was decreasing as the test progressed and senescence increased from day 21 of regrowth.</span>展开更多
In recent years,a large number of differentially expressed genes have been identified in human umbilical cord mesenchymal stem cell(hUMSC)transplants for the treatment of ischemic cerebral infarction.These genes are i...In recent years,a large number of differentially expressed genes have been identified in human umbilical cord mesenchymal stem cell(hUMSC)transplants for the treatment of ischemic cerebral infarction.These genes are involved in various biochemical processes,but the role of microRNAs(miRNAs)in this process is still unclear.From the Gene Expression Omnibus(GEO)database,we downloaded two microarray datasets for GSE78731(messenger RNA(mRNA)profile)and GSE97532(miRNA profile).The differentially expressed genes screened were compared between the hUMSC group and the middle cerebral artery occlusion group.Gene ontology enrichment and pathway enrichment analyses were subsequently conducted using the online Database for Annotation,Visualization,and Integrated Discovery.Identified genes were applied to perform weighted gene co-suppression analyses,to establish a weighted co-expression network model.Furthermore,the protein-protein interaction network for differentially expressed genes from turquoise modules was built using Cytoscape(version 3.40)and the most highly correlated subnetwork was extracted from the protein-protein interaction network using the MCODE plugin.The predicted target genes for differentially expressed miRNAs were also identified using the online database starBase v3.0.A total of 3698 differentially expressed genes were identified.Gene ontology analysis demonstrated that differentially expressed genes that are related to hUMSC treatment of ischemic cerebral infarction are involved in endocytosis and inflammatory responses.We identified 12 differentially expressed miRNAs in middle cerebral artery occlusion rats after hUMSC treatment,and these differentially expressed miRNAs were mainly involved in signaling in inflammatory pathways,such as in the regulation of neutrophil migration.In conclusion,we have identified a number of differentially expressed genes and differentially expressed mRNAs,miRNA-mRNAs,and signaling pathways involved in the hUMSC treatment of ischemic cerebral infarction.Bioinformatics and interaction analyses can provide novel clues for further research into hUMSC treatment of ischemic cerebral infarction.展开更多
文摘The objective of the present investigation was to evaluate the<i><span style="font-family:Verdana;"> Urochloa</span></i><span style="font-family:Verdana;"> Insurgent, Piata and Signal grasses by varying the phenology in the attributes: stem population dynamics, tissue replacement, leaf:stem relation and weight per stem. The data were analyzed using a completely randomized block design with arrangement in divided plots and four replications, the procedure used was PROC GLM from SAS. The Signal grass presented higher stem density with an average of 450 m</span><sup><span style="font-family:Verdana;">?2</span></sup><span style="font-family:Verdana;"> stems, while the meadow with Insurgent grass registered the lowest stem density throughout the investigation, with an average of 320 m</span><sup><span style="font-family:Verdana;">-2</span></sup><span style="font-family:Verdana;"> stems;furthermore, in this treatment, the stem density tended to increase slowly over time (P = 0.05). The Insurgente and signal grasses showed rapid leaf elongation from day 21 of regrowth with 123 and 104 cm stem</span><sup><span style="font-family:Verdana;">?1</span></sup><span style="font-family:Verdana;">, while in Piata the accelerated leaf elongation was on day 14 with 113 cm stem</span><sup><span style="font-family:Verdana;">?1</span></sup><span style="font-family:Verdana;"> where it remained active its growth until day 56 (P > 0.05). As the regrowth age was increasing, the population dynamics of stems and weight per stem were increasing;in Insurgent and signal the leaf elongation and net growth increased to a maximum point to start declining;however, in Piata grass was increasing without decreasing. The trend of the leaf:stem relation was decreasing as the test progressed and senescence increased from day 21 of regrowth.</span>
基金supported by the National Key Research&Development Program of China,No.2016YFC1301600Program for Jilin University Science and Technology Innovation Team,No.2017TD-12(both to YY)
文摘In recent years,a large number of differentially expressed genes have been identified in human umbilical cord mesenchymal stem cell(hUMSC)transplants for the treatment of ischemic cerebral infarction.These genes are involved in various biochemical processes,but the role of microRNAs(miRNAs)in this process is still unclear.From the Gene Expression Omnibus(GEO)database,we downloaded two microarray datasets for GSE78731(messenger RNA(mRNA)profile)and GSE97532(miRNA profile).The differentially expressed genes screened were compared between the hUMSC group and the middle cerebral artery occlusion group.Gene ontology enrichment and pathway enrichment analyses were subsequently conducted using the online Database for Annotation,Visualization,and Integrated Discovery.Identified genes were applied to perform weighted gene co-suppression analyses,to establish a weighted co-expression network model.Furthermore,the protein-protein interaction network for differentially expressed genes from turquoise modules was built using Cytoscape(version 3.40)and the most highly correlated subnetwork was extracted from the protein-protein interaction network using the MCODE plugin.The predicted target genes for differentially expressed miRNAs were also identified using the online database starBase v3.0.A total of 3698 differentially expressed genes were identified.Gene ontology analysis demonstrated that differentially expressed genes that are related to hUMSC treatment of ischemic cerebral infarction are involved in endocytosis and inflammatory responses.We identified 12 differentially expressed miRNAs in middle cerebral artery occlusion rats after hUMSC treatment,and these differentially expressed miRNAs were mainly involved in signaling in inflammatory pathways,such as in the regulation of neutrophil migration.In conclusion,we have identified a number of differentially expressed genes and differentially expressed mRNAs,miRNA-mRNAs,and signaling pathways involved in the hUMSC treatment of ischemic cerebral infarction.Bioinformatics and interaction analyses can provide novel clues for further research into hUMSC treatment of ischemic cerebral infarction.
