Stem cell transplantation and low-energy shock-wave therapy （LESWT） have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in di...Stem cell transplantation and low-energy shock-wave therapy （LESWT） have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in diabetic erectile dysfunction models, the number of visible stem cells was rather low and decreased quickly. LESWT could recruit endogenous stem cells to the cavernous body and improve the microenvironment in diabetic cavernous tissue. Thus, we deduced that LESWT might benefit transplanted stem cell survival and improve the effects of stem cell transplantation. In this research, 42 streptozotocin-induced diabetic rats were randomized into four groups： the diabetic group （n = 6）, the LESWT group （n = 6）, the bone marrow-derived mesenchymal stem cell （BMSC） transplantation group （n = 15）, and the combination of LESWT and BMSC transplantation group （n = 15）. One and three days after BMSC transplantation, three rats were randomly chosen to observe the survival numbers of BMSCs in the cavernous body. Four weeks after BMSC transplantation, the following parameters were assessed： the surviving number of transplanted BMSCs in the cavernous tissue, erectile function, real-time polymerase chain reaction, and penile immunohistochemical assessment. Our research found that LESWT favored the survival of transplanted BMSCs in the cavernous body, which might be related to increased stromal cell-derived factor-1 expression and the enhancement of angiogenesis in the diabetic cavernous tissue. The combination of LESWT and BMSC transplantation could improve the erectile function of diabetic erectile function rats more effectively than LESWT or BMSC transplantation performed alone.展开更多
目的探究超短波对颌骨骨髓间充质干细胞(JBMSCs)增殖、凋亡和成骨分化特性的影响。方法原代提取培养C57BL/6N小鼠JBMSCs并鉴定。取第三代JBMSCs进行实验,将细胞接种于培养板并随机分为非超短波处理组(Con)和超短波处理组(USW),超短波处...目的探究超短波对颌骨骨髓间充质干细胞(JBMSCs)增殖、凋亡和成骨分化特性的影响。方法原代提取培养C57BL/6N小鼠JBMSCs并鉴定。取第三代JBMSCs进行实验,将细胞接种于培养板并随机分为非超短波处理组(Con)和超短波处理组(USW),超短波处理组根据处理时长不同分为5组:30 s组、1 min组、3 min组、5 min组和7 min组,每天按照分组处理1次,通过CCK-8和qRT-PCR检测细胞增殖;Western blot、qRT-PCR检测凋亡相关标志物Bax、Bcl-2的表达,成骨相关标志物Runx2、ALP的表达。结果CCK-8结果显示,3 min USW处理JBMSCs可显著增强其第3天和第5天的OD值,3 min USW可上调Bcl-2的蛋白和mRNA表达水平,下调Bax的蛋白和mRNA表达水平;此外,3 min USW可有效上调ALP、Runx2的蛋白和mRNA表达水平。结论3 min USW处理能有效提高JBMSCs增殖和成骨分化能力并抑制其凋亡。展开更多
文摘Stem cell transplantation and low-energy shock-wave therapy （LESWT） have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in diabetic erectile dysfunction models, the number of visible stem cells was rather low and decreased quickly. LESWT could recruit endogenous stem cells to the cavernous body and improve the microenvironment in diabetic cavernous tissue. Thus, we deduced that LESWT might benefit transplanted stem cell survival and improve the effects of stem cell transplantation. In this research, 42 streptozotocin-induced diabetic rats were randomized into four groups： the diabetic group （n = 6）, the LESWT group （n = 6）, the bone marrow-derived mesenchymal stem cell （BMSC） transplantation group （n = 15）, and the combination of LESWT and BMSC transplantation group （n = 15）. One and three days after BMSC transplantation, three rats were randomly chosen to observe the survival numbers of BMSCs in the cavernous body. Four weeks after BMSC transplantation, the following parameters were assessed： the surviving number of transplanted BMSCs in the cavernous tissue, erectile function, real-time polymerase chain reaction, and penile immunohistochemical assessment. Our research found that LESWT favored the survival of transplanted BMSCs in the cavernous body, which might be related to increased stromal cell-derived factor-1 expression and the enhancement of angiogenesis in the diabetic cavernous tissue. The combination of LESWT and BMSC transplantation could improve the erectile function of diabetic erectile function rats more effectively than LESWT or BMSC transplantation performed alone.
文摘目的探究超短波对颌骨骨髓间充质干细胞(JBMSCs)增殖、凋亡和成骨分化特性的影响。方法原代提取培养C57BL/6N小鼠JBMSCs并鉴定。取第三代JBMSCs进行实验,将细胞接种于培养板并随机分为非超短波处理组(Con)和超短波处理组(USW),超短波处理组根据处理时长不同分为5组:30 s组、1 min组、3 min组、5 min组和7 min组,每天按照分组处理1次,通过CCK-8和qRT-PCR检测细胞增殖;Western blot、qRT-PCR检测凋亡相关标志物Bax、Bcl-2的表达,成骨相关标志物Runx2、ALP的表达。结果CCK-8结果显示,3 min USW处理JBMSCs可显著增强其第3天和第5天的OD值,3 min USW可上调Bcl-2的蛋白和mRNA表达水平,下调Bax的蛋白和mRNA表达水平;此外,3 min USW可有效上调ALP、Runx2的蛋白和mRNA表达水平。结论3 min USW处理能有效提高JBMSCs增殖和成骨分化能力并抑制其凋亡。
