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Intra-annual stem diameter growth of Tamarix ramosissima and association with hydroclimatic factors in the lower reaches of China's Heihe River 被引量:12
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作者 ShengChun XIAO HongLang XIAO +1 位作者 XiaoMei PENG QuanYan TIAN 《Journal of Arid Land》 SCIE CSCD 2014年第4期498-510,共13页
High-resolution observations of cambial phenology and intra-annual growth dynamics are useful approaches for understanding the response of tree growth to climate and environmental change. During the past two decades, ... High-resolution observations of cambial phenology and intra-annual growth dynamics are useful approaches for understanding the response of tree growth to climate and environmental change. During the past two decades, rapid socioeconomic development has increased the demand for water resources in the oases of the middle reaches of the Heihe River in northwestern China, and the lower reaches of the Heihe River have changed from a perennial river to an ephemeral stream with a decreased and degraded riparian zone. Tamarisk(Tamarix ramosissima) is the dominant shrub species of the desert riparian forest. In this study, the daily and seasonal patterns of tamarisk stem diameter growth, including the main period of tree ring formation, were examined. Observations concerning the driving forces of growth changes, along with implications for the ecology of the dendrohydrological area and management of desert riparian forests in similar arid regions, are also presented. The diurnal-seasonal activity of stem diameter and the dynamics of growth ring formation were studied using a point dendrometer and micro-coring methods during the 2012 growing season in shrub tamarisk in a desert riparian forest stand in the lower reaches of the Heihe River in Ejin Banner, Inner Mongolia of northwestern China. Generally, the variation in diurnal diameter of tamarisk was characterized by an unstable multi-peak pattern, with the cumulative stem diameter growth over the growing season following an S-shaped curve. The period from late May to early August was the main period of stem diameter growth and growth-ring formation. Among all of the hydroclimatic factors considered in this study, only groundwater depth was significantly correlated with stem diameter increment during this period. Therefore, for the dendrochronological study, the annual rings of the tamarisk can be used to reconstruct processes that determine the regional water regime, such as river runoff and fluctuations in groundwater depth. For the management of desert riparian forests, suitable groundwater depths must be maintained in the spring and summer to sustain tree health and a suitable stand structure. 展开更多
关键词 Tamarix ramosissima stem diameter growth tree ring formation hydroclimatic factors diurnal-seasonal scale Heihe River
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Suitability of Stem Diameter Variations as an Indicator of Water Stress of Cotton 被引量:3
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作者 ZHANG Ji-yang DUAN Ai-wang MENG Zhao-jiang LIU Zu-gui 《Agricultural Sciences in China》 CAS CSCD 2006年第5期356-362,共7页
Water stress effects on stem diameter variations (SDV) were studied in a pot experiment on cotton (Gossypium hirustum L. Meimian99B). Water restriction was imposed at the flowering stage and were compared with a w... Water stress effects on stem diameter variations (SDV) were studied in a pot experiment on cotton (Gossypium hirustum L. Meimian99B). Water restriction was imposed at the flowering stage and were compared with a well-watered control treatment. The volumetric soil water content (0v) and SDV were monitored continuously. The objective was to determine the feasibility of using the parameters derived from stem diameter measurements, including maximum daily stem shrinkage (MDS), maximum daily stem diameter (MXSD), and minimum daily stem diameter (MNSD) as indicators of plant water stress. The different behavior of SDV was founded at different growth stages. At stem-maturing stage, MDS increased and MNSD decreased in deficit-irrigated plants compared with the control plants, therefore, it appeared that MDS and MNSD ccould be used as available indicators of plant water status. At stem growth stage, there were no significant differences in MDS values between treatments but MXSD and MNSD responded sharply to soil water deficits. Thus, for rapidly growing cotton, the course of MXSD or MNSD with time offered a consistent stress indicator. SDV was also closely related to atmospheric factors, solar radiation (Rs) and vapor pressure deficit (VPD) were found to be the predominant factors affecting MDS, followed by the relative humidity (RH), while air temperature (Ta) and wind velocity had the least effect. A good linear relationship was founded (r^2 = 0.921) between MDS and environmental variables (Rs, VPD, RH, and θv), which can be used to establish a reference value for detecting plant water stress based on the MDS patterns. 展开更多
关键词 stem diameter variations water stress atmospheric factors COTTON
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Estimating Pinus palustris tree diameter and stem volume from tree height,crown area and stand-level parameters 被引量:15
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作者 C.A.Gonzalez-Benecke Salvador A.Gezan +3 位作者 Lisa J.Samuelson Wendell P.Cropper Daniel J.Leduc Timothy A.Martin 《Journal of Forestry Research》 SCIE CAS CSCD 2014年第1期43-52,共10页
Accurate and efficient estimation of forest growth and live biomass is a critical element in assessing potential responses to forest management and environmental change. The objective of this study was to develop mode... Accurate and efficient estimation of forest growth and live biomass is a critical element in assessing potential responses to forest management and environmental change. The objective of this study was to develop models to predict longleaf pine tree diameter at breast height (dbh) and merchantable stem volume (V) using data obtained from field measurements. We used longleaf pine tree data from 3,376 planted trees on 127 permanent plots located in the U.S. Gulf Coastal Plain region to fit equations to predict dbh and V as functions of tree height (H) and crown area (CA). Prediction of dbh as a function of H improved when CA was added as an additional independent variable. Similarly, predic- tions of V based on H improved when CA was included. Incorporation of additional stand variables such as age, site index, dominant height, and stand density were also evaluated but resulted in only small improvements in model performance. For model testing we used data from planted and naturally-regenerated trees located inside and outside the geographic area used for model fitting. Our results suggest that the models are a robust alternative for dbh and V estimations when H and CA are known on planted stands with potential for naturally-regenerated stands, across a wide range of ages. We discuss the importance of these models for use with metrics derived from remote sensing data. 展开更多
关键词 Longleaf pine diameter-height relationships crown area individual-tree stem volume growth and yield modeling
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Effects of Different Ridge Culture Ways on Stem Diameter,Plant Height,the Number of Leaves and Yield of Phaseolus vulgaris L. under Dry Farming Conditions 被引量:1
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作者 NIE Hong-mei1,XING Guo-ming1,ZHENG Shao-wen1,WEN Bian-ying21. College of Horticulture,Shanxi Agricultural University,Taigu 030801 2. Vegetables Office in Yangqu County,Shanxi Province,Yangqu 030100 《Agricultural Science & Technology》 CAS 2010年第5期59-61,共3页
The variety "Aifeng" was used as experimental material,and the stem diameter,plant height,the number of leaves and yield of P. vulgaris under three ways of ridge culture (bedding,high ridge and M ridge) were... The variety "Aifeng" was used as experimental material,and the stem diameter,plant height,the number of leaves and yield of P. vulgaris under three ways of ridge culture (bedding,high ridge and M ridge) were observed and detected to study the effects of different ridge cultures on the growth and yield. The results showed that the stem diameter,plant height,the number of leaves and yield under M ridge culture were higher than that of bedding and high ridge. 展开更多
关键词 Phaseolus vulgaris L. Dry farming Ridge culture
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Measuring tree stem diameters and straightness with depth-image computer vision
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作者 Hoang Tran Keith Woeste +2 位作者 Bowen Li Akshat Verma Guofan Shao 《Journal of Forestry Research》 SCIE CAS CSCD 2023年第5期1395-1405,共11页
Current techniques of forest inventory rely on manual measurements and are slow and labor intensive.Recent developments in computer vision and depth sensing can produce accurate measurement data at significantly reduc... Current techniques of forest inventory rely on manual measurements and are slow and labor intensive.Recent developments in computer vision and depth sensing can produce accurate measurement data at significantly reduced time and labor costs.We developed the ForSense system to measure the diameters of trees at various points along the stem as well as stem straightness.Time use,mean absolute error(MAE),and root mean squared error(RMSE)metrics were used to compare the system against manual methods,and to compare the system against itself(reproducibility).Depth-derived diameter measurements of the stems at the heights of 0.3,1.4,and 2.7 m achieved RMSE of 1.7,1.5,and 2.7 cm,respectively.The ForSense system produced straightness measurement data that was highly correlated with straightness ratings by trained foresters.The ForSense system was also consistent,achieving sub-centimeter diameter difference with subsequent measures and less than 4%difference in straightness value between runs.This method of forest inventory,which is based on depth-image computer vision,is time efficient compared to manual methods and less computationally and technologically intensive compared to Structure-from-Motion(SFM)photogrammetry and ground-based LiDAR or terrestrial laser scanning(TLS). 展开更多
关键词 Forest inventory Depth sensing Computer vision Tree diameter stem straightness Trunk volume
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Habitat use by mountain nyala Tragelaphus buxtoni determined using stem bite diameters at point of browse, bite rates, and time budgets in the Bale Mountains National Park, Ethiopia
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作者 Solomon A. TADESSE Butt P. KOTLER 《Current Zoology》 SCIE CAS CSCD 2013年第6期707-717,共11页
We studied the habitat use of mountain nyala Tragelaphus buxtoni in the northern edge of the Bale Mountains Na- tional Park, Ethiopia. The aims of this study were to: (1) measure and quantify habitat-specific stem ... We studied the habitat use of mountain nyala Tragelaphus buxtoni in the northern edge of the Bale Mountains Na- tional Park, Ethiopia. The aims of this study were to: (1) measure and quantify habitat-specific stem bite diameters of mountain nyala foraging on common natural plant species in two major habitat types (i.e. grasslands versus woodlands), and (2) quantify the bite rates (number of bites per minute) and the activity time budgets of mountain nyala as functions of habitat type and sex-age category. We randomly laid out three transects in each habitat type. Following each transect, through focal animal obser- vations, we assessed and quantified stem diameters at point of browse (dpb), bite rates, and time budgets of mountain nyala in grasslands versus woodlands. Stem dpb provide a measure of natural giving-up densities (GUDs) and can be used to assess fora- ging costs and efficiencies, with greater stem dpb corresponding to lower costs and greater efficiencies. The results showed that stem dpb, bite rates, induced vigilance, and proportion of time spent in feeding differed between habitats. In particular, mountain nyala had greater stem dpb, higher bite rates, and spent a greater proportion of their time in feeding and less in induced vigilance in the grasslands. In addition, adult females had the highest bite rates, and the browse species Solanum marginatum had the greatest stem dpb. Generally, grasslands provide the mountain nyala with several advantages over the woodlands, including of- fering lower foraging costs, greater safety, and more time for foraging. The study advocates how behavioural indicators and natural GUDs are used to examine the habitat use of the endangered mountain nyala through applying non-invasive techniques. We conclude that the resulting measures are helpful for guiding conservation and management efforts and could be applicable to a number of endangered wildlife species including the mountain nyala . 展开更多
关键词 Behavioural indicators Bite rates Mountain nyala Natural giving-up densities Non-invasive techniques stem bite diameters Vigilance
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Survivorship, attained diameter, height and volume of three Paulownia species after 9 years in the southern Appalachians, USA
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作者 Erik C.Berg Stanley J.Zarnoch W.Henry McNab 《Journal of Forestry Research》 SCIE CAS CSCD 2020年第6期2181-2191,共11页
Little is known of the tree and stand dynamics of varied species of planted Paulownia left unmanaged until harvest in the southeastern United States.We sought to remedy this lack of information needed by land managers... Little is known of the tree and stand dynamics of varied species of planted Paulownia left unmanaged until harvest in the southeastern United States.We sought to remedy this lack of information needed by land managers to make informed decisions by investigating diff erences in survivorship,attained diameter breast height(DBH),diameter at ground level,total height,tree volume and standlevel volume yields of planted P.elongata,P.fortunei,and P.tomentosa in the cool-moist environment of the southern Appalachian Mountains.After 9 years,combined-species survivorship was only 27.3%.Low survivorship was likely related to several inclement weather events.P.fortunei was signifi cantly smaller in DBH and total height.Three combined-species stem(bole)volume models were developed as functions of(1)DBH squared,(2)the product DBH squared and total height,and(3)the product diameter ground line squared and total height.Mean total volume production of unmanaged stands was greatest for P.elongata and P.fortunei 4 years after planting;by the 9th year,total volume of P.elongata was greater than the other two species.Results of our study provide managers information on productivity of three species of Paulownia that can be used for estimating plantation yields. 展开更多
关键词 diameter Tree height Stand structure stem volume PAULOWNIA
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雌雄大鼠脂肪间充质干细胞代谢、增殖、分化及向血管平滑肌细胞分化的差异性
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作者 王秋花 杜孜玮 +2 位作者 王文双 赵冬梅 张晓晴 《中国组织工程研究》 北大核心 2026年第7期1687-1698,共12页
背景:在血管壁结构中,血管平滑肌细胞通过调节血管张力维持压力稳定,确保组织血液供应。血管平滑肌细胞具有高度可塑性,是血管再生的关键细胞类型。然而,成熟的血管平滑肌细胞难以获取且体外扩增能力有限。相比之下,脂肪间充质干细胞易... 背景:在血管壁结构中,血管平滑肌细胞通过调节血管张力维持压力稳定,确保组织血液供应。血管平滑肌细胞具有高度可塑性,是血管再生的关键细胞类型。然而,成熟的血管平滑肌细胞难以获取且体外扩增能力有限。相比之下,脂肪间充质干细胞易于获取、扩增能力强,并具备向血管平滑肌细胞分化的潜能。目前,雌雄来源脂肪间充质干细胞的代谢活性、增殖、集落形成等生物学特性及向血管平滑肌细胞分化的能力是否存在差异仍需深入研究。目的:比较雌性和雄性大鼠脂肪间充质干细胞的代谢活性、增殖、分化能力等生物学特征及向血管平滑肌细胞分化潜能的差异。方法:①分别从雌、雄大鼠腹股沟区获取脂肪组织,采用胶原酶消化法提取脂肪间充质干细胞,通过流式细胞术检测表面抗原(CD90、CD29、CD45),并利用WST-1实验、细胞倍增实验、集落形成实验及成脂、成骨、成软骨分化实验比较雌雄大鼠脂肪间充质干细胞的生物学特征。②用5 ng/mL转化生长因子β诱导雌雄大鼠脂肪间充质干细胞向血管平滑肌细胞分化,通过qRT-PCR检测α-SMA、SM22α、Calponin、Caldesmon、SMMHC、Smoothelin基因表达,免疫荧光染色检测α-SMA、Caldesmon、Smoothelin蛋白表达,并通过胶原晶格收缩实验评估血管平滑肌细胞的收缩功能。结果与结论:①原代培养3 d后,显微镜下观察雌性和雄性大鼠脂肪干细胞形态均一,呈纺锤状;②流式细胞术检测大鼠脂肪间充质干细胞高表达CD90、CD29,低表达CD45;③与雄性大鼠脂肪间充质干细胞相比,雌性大鼠脂肪间充质干细胞代谢活性更好(P<0.05);④雌性和雄性大鼠脂肪间充质干细胞在倍增时间、集落形成、成脂、成骨、成软骨分化能力方面相似;⑤向血管平滑肌细胞诱导分化后,在基因层面,相较于雄性大鼠脂肪间充质干细胞,雌性大鼠脂肪间充质干细胞α-SMA、Caldesmon和SMMHC高表达(P<0.05);在蛋白层面,相较于雄性大鼠脂肪间充质干细胞,雌性大鼠脂肪间充质干细胞α-SMA高表达(P<0.05);在收缩功能层面,雌性和雄性大鼠脂肪间充质干细胞在收缩功能上相似。由此可见,在代谢活性和向血管平滑肌细胞分化方面,雌性大鼠脂肪间充质干细胞更有优势。 展开更多
关键词 脂肪间充质干细胞 血管平滑肌细胞 诱导分化 雌性 雄性 小口径人工血管
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Comparison of stem taper models for the four tropical tree species in Mount Makiling, Philippines 被引量:4
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作者 Roscinto Ian C.LUMBRES Azyleah C.ABINO +2 位作者 Nelson M.PAMPOLINA Feliciano G.CALORA Jr Young Jin LEE 《Journal of Mountain Science》 SCIE CSCD 2016年第3期536-545,共10页
This study was conducted to evaluate the performance of six stem taper models on four tropical tree species,namely Celtis luzonica(Magabuyo),Diplodiscus paniculatus(Balobo),Parashorea malaanonan(Bagtikan),and Swieteni... This study was conducted to evaluate the performance of six stem taper models on four tropical tree species,namely Celtis luzonica(Magabuyo),Diplodiscus paniculatus(Balobo),Parashorea malaanonan(Bagtikan),and Swietenia macrophylla(Mahogany)in Mount Makiling Forest Reserve(MMFR),Philippines using fit statistics and lack-of-fit statistics.Four statistical criteria were used in this study,including the standard error of estimate(SEE),coefficient of determination(R^2),mean bias(E),and absolute mean difference(AMD).For the lack-offit statistics,SEE,E and AMD were determined in different relative height classes.The results indicated that the Kozak02 stem taper model offered the best fit for the four tropical species in most statistics.The Kozak02 model also consistently provided the best performance in the lack-of-fit statistics with the best SEE,E and AMD in most of the relative height classes.These stem taper equations could help forest managers and researchers better estimate the diameter of the outside bark with any given height,merchantable stem volumes and total stem volumes of standing trees belonging to the four species of thetropical forest in MMFR. 展开更多
关键词 Mount Makiling Forest Reserve stem volume estimation diameter outside bark Kozak model Tropical tree species
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Comparative Analysis of Four Stem Taper Models for Quercus glauca in Mount Halla,Jeju Island,South Korea 被引量:1
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作者 LUMBRES Roscinto Ian Canicosa LEE Young Jin +6 位作者 CHOI Hyung Soon KIM Sung Yong JANG Mi Na ABINO Azyleah Caizares SEO Yeon Ok KIM Chan Soo PARK Jung Hwan 《Journal of Mountain Science》 SCIE CSCD 2014年第2期442-448,共7页
This study was conducted to fit the diameter-height data of Quercusglaucain Jeju Island, South Korea to the four commonly used stem taper equations andto evaluate the performance of the four stem taper models using fo... This study was conducted to fit the diameter-height data of Quercusglaucain Jeju Island, South Korea to the four commonly used stem taper equations andto evaluate the performance of the four stem taper models using four statistical criteria: Fit index (FI), root mean square error (RMSE), bias (),and absolute mean difference (AMD). Results showed that the Kozak02stem taper equation provided the best FI(0.9847), RMSE(1.5745),(-0.0030 cm) and AMD (1.0990 cm) whileMax and Burkhart model had the poorest performance among the four stem taper models based on the four evaluation statistics (FI : 0.9793,RMSE : 1.8272, : 0.3040 cm and AMD : 1.3060 cm). These stem taper equations can serve as a useful tool for forest managers in estimating the diameter outside bark at any given height, merchantable stem volumes and total stem volumesof the standing trees of Quercusglaucain theGotjawal forests located in Mount Halla, Jeju Island, South Korea. 展开更多
关键词 MountHalla Merchantable stem volume diameter outside bark Kozak model Model evaluation
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Evaluation of Some Stem Taper Models for Camellia japonica in Mount Halla, Korea
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作者 Sung Cheol JUNG Yeon Ok SEO +1 位作者 Hyun Kyu WON Roscinto Ian C.LUMBRES 《Journal of Mountain Science》 SCIE CSCD 2015年第6期1395-1402,共8页
This study was conducted to evaluate the performance of the four stem taper models on Camellia japonica in Jeju Island, Korea using fit statistics and lack-of-fit statistics. The five statistical criteria that were us... This study was conducted to evaluate the performance of the four stem taper models on Camellia japonica in Jeju Island, Korea using fit statistics and lack-of-fit statistics. The five statistical criteria that were used in this study were standard error of estimate(SEE), mean bias( E), absolute mean difference(AMD), coefficient of determination(R2), and root mean square error(RMSE). Results showed that the Kozak model 02 stem taper had the best performance in all fit statistics(SEE: 3.4708, E : 0.0040 cm, AMD : 0.9060 cm, R2 : 0.9870, and RMSE : 1.2545). On the other hand, Max and Burkhart stem taper model had the poorest performance in each statistical criterion(SEE: 4.2121, E : 0.2520 cm, AMD : 1.1300 cm, R2 : 0.9805, and RMSE: 1.5317). For the lack-of-fit statistics, the Kozak model 02 also provided the best performance having the best AMD in most of the relative height classes for diameter outside bark prediction and in most of the DBH classes for total volume prediction while Max and Burkhart had the poorest performance. These stem taper equations could help forest managers to better estimate the diameter outside bark at any given height, merchantable stem volumes and total stem volumes of the standing trees of Camellia japonica in the forests of Jeju Island, Korea. 展开更多
关键词 Mount Halla stem volume diameter outside bark Kozak model Model evaluation
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测交鉴定结合数量性状遗传分析创制糯高粱保持系资源
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作者 韩永亮 方路斌 +3 位作者 罗河月 崔江慧 郭群 常金华 《湖北农业科学》 2025年第8期54-61,共8页
生产上三系杂交糯高粱的应用一直因优良糯性保持系种质资源缺乏而受到制约,为了快速创制高粱糯性保持系种质资源,以矮脚糯高粱为父本、保持系材料TX623B为母本,构建F_(2)分离群体及F_(2:3)家系群体,采用主基因+多基因混合模型分析高粱... 生产上三系杂交糯高粱的应用一直因优良糯性保持系种质资源缺乏而受到制约,为了快速创制高粱糯性保持系种质资源,以矮脚糯高粱为父本、保持系材料TX623B为母本,构建F_(2)分离群体及F_(2:3)家系群体,采用主基因+多基因混合模型分析高粱数量性状株高和茎粗的遗传规律。结果表明,株高受2对主基因控制,遗传力较高;茎粗受1对主基因控制,遗传力偏低。基于遗传分析结果,采用DPS数据处理系统建立了基于模糊相似优先比的加权综合评价筛选体系,并对F_(2:3)家系进行了加权综合评价,通过综合评价对优良家系材料在品种选育中的应用方向进行了评估,筛选出综合性状良好且表型性状接近TX623B的糯保持系材料,为高粱品种选育与杂种优势利用工作提供了方法依据和材料基础。 展开更多
关键词 糯高粱 保持系 回交转育 测交 数量性状 株高 茎粗 主基因+多基因混合遗传模型
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滇中亚高山不同径级米槠树干液流昼夜特征及其影响因素 被引量:2
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作者 丘雪明 王克勤 +3 位作者 和娴越 李珠宇 张广贺 赵洋毅 《应用生态学报》 北大核心 2025年第1期21-30,共10页
以滇中亚高山磨盘山森林公园内天然常绿阔叶树种米槠为研究对象,采用TDP-30热扩散探测器和小型自动气象站对米槠树干液流及林地环境因子进行连续原位监测,研究米槠树干液流昼夜变化特征及其影响因素。结果表明:米槠大(35~45 cm)、中(25~... 以滇中亚高山磨盘山森林公园内天然常绿阔叶树种米槠为研究对象,采用TDP-30热扩散探测器和小型自动气象站对米槠树干液流及林地环境因子进行连续原位监测,研究米槠树干液流昼夜变化特征及其影响因素。结果表明:米槠大(35~45 cm)、中(25~35 cm)、小(15~25 cm)3个径级的树干液流速率日变化均呈明显的“昼高夜低”单峰曲线,且前半夜液流速率略高于后半夜。日尺度上液流速率变化均呈先上升后下降的趋势,整体变化幅度与径级大小呈正比,各径级液流速率大小依次为中径级>大径级>小径级。各径级整日液流量为19.21~72.27 mL·cm^(-2)·d^(-1),夜间液流量为0.97~6.04 mL·cm^(-2)·d^(-1),夜间液流对整日液流的贡献率为2.6%~21.5%,各径级夜间液流贡献率大小依次为小径级>中径级>大径级。米槠树干液流速率与太阳辐射、饱和水汽压亏缺、空气温度、相对湿度和风速显著相关,其中,昼间液流速率的主要影响因子是太阳辐射、饱和水汽压亏缺、空气温度、相对湿度和风速,夜间液流速率的主要影响因子是空气温度。在我国西南亚高山低温高湿生境下,米槠树干液流存在明显的昼夜变化规律,且昼间和夜间的树干液流影响因素存在明显差异;在估算米槠日总蒸腾耗水量时,不可忽视夜间液流的影响。 展开更多
关键词 亚高山森林 米槠 径级 昼夜树干液流 环境影响
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不同静电纺丝膜上骨髓间充质干细胞的黏附、增殖与成血管平滑肌分化 被引量:4
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作者 孙现娟 王秋花 +3 位作者 张锦艺 杨杨杨 王文双 张晓晴 《中国组织工程研究》 CAS 北大核心 2025年第4期661-669,共9页
背景:临床上迫切需要小口径人工血管来治疗冠状动脉和外周动脉疾病,目前,血管组织工程已成为制备小口径人工血管的主要方法,选择合适的生物材料和细胞来源是小口径组织工程血管构建成功的关键因素。目的:观察4种静电纺丝膜材料对骨髓间... 背景:临床上迫切需要小口径人工血管来治疗冠状动脉和外周动脉疾病,目前,血管组织工程已成为制备小口径人工血管的主要方法,选择合适的生物材料和细胞来源是小口径组织工程血管构建成功的关键因素。目的:观察4种静电纺丝膜材料对骨髓间充质干细胞增殖、黏附及分化为血管平滑肌细胞的影响。方法:分离提取SD大鼠骨髓间充质干细胞。将骨髓间充质干细胞分别接种于聚己内酯(PCL)、聚己内酯-透明质酸(PCL-HA)、聚己内酯-丝素蛋白(PCL-SF)、聚己内酯-明胶(PCL-GEL)静电纺丝膜材料上,培养1,3,7 d后,扫描电镜下观察材料上的细胞排布,鬼笔环肽染色观察材料上的细胞增殖与黏附,qRT-PCR检测材料上细胞分泌的CD90、Meflin、转化生长因子βmRNA表达;向血管平滑肌细胞诱导分化7 d后,qRT-PCR检测材料上细胞ɑ-平滑肌肌动蛋白mRNA表达。结果与结论:①扫描电镜下可见骨髓间充质干细胞在4种静电纺丝膜上均沿着静电纺丝膜的纤维走向排列;②鬼笔环肽染色显示,骨髓间充质干细胞在4种静电纺丝膜上分布规律,均沿着纤维走向呈现平行分布,并且PCL-HA、PCL-SF、PCL-GEL静电纺丝膜较PCL静电纺丝膜更有利于骨髓间充质干细胞的增殖、黏附,PCL-SF静电纺丝膜相较于PCL-HA、PCL-GEL静电纺丝膜更有利于骨髓间充质干细胞的增殖、黏附;③qRT-PCR检测显示,4种静电纺丝膜材料均可维持骨髓间充质干细胞CD90和Meflin的mRNA表达,组间比较差异无显著性意义(P>0.05);PCL-HA、PCL-SF、PCL-GEL组培养1,7 d的转化生长因子βmRNA表达高于PCL组(P<0.05),PCL-SF组培养3,7 d的转化生长因子βmRNA表达高于其他3组(P<0.05),PCL-HA组培养7 d的转化生长因子βmRNA表达高于PCL-GEL组(P<0.05);④qRT-PCR检测显示,PCL-SF组ɑ-平滑肌肌动蛋白mRNA表达高于其他3组(P<0.05),PCL-HA组ɑ-平滑肌肌动蛋白mRNA表达高于PCL组(P<0.05);⑤结果表明:相较于PCL、PCL-HA、PCL-GEL静电纺丝膜,PCL-SF静电纺丝膜与骨髓间充质干细胞结合更适合制备小口径组织工程血管。 展开更多
关键词 骨髓间充质干细胞 小口径组织工程血管 静电纺丝 聚己内酯 聚己内酯-透明质酸 聚己内酯-丝素蛋白 聚己内酯-明胶
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设施促成栽培条件下不同序位坐果对梨果实品质的影响 被引量:1
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作者 蔡丹英 戴美松 +2 位作者 王月志 魏春艳 施泽彬 《浙江农业科学》 2025年第5期1091-1095,共5页
为了探明设施促成栽培条件下不同序位坐果对梨果实品质的影响,以塑料大棚栽培翠冠、翠玉、新玉梨为试材,分析保留不同花序序位果实时其成熟果实的表型指标及其相关性。结果表明:翠冠、翠玉和新玉的最大单果重分别在第4、第3和第1序位;... 为了探明设施促成栽培条件下不同序位坐果对梨果实品质的影响,以塑料大棚栽培翠冠、翠玉、新玉梨为试材,分析保留不同花序序位果实时其成熟果实的表型指标及其相关性。结果表明:翠冠、翠玉和新玉的最大单果重分别在第4、第3和第1序位;翠冠和翠玉果实横径和纵径分别是第4和第3序位最大,新玉果实横径则为第1序位最大,纵径为第3序位最大;不同序位留果对翠冠和翠玉果形指数的影响均不显著,新玉的果形指数则随着序位的升高而升高;翠冠、翠玉和新玉的果柄均为第3序位最粗,第5序位最细;翠冠、翠玉和新玉的果实可溶性固形物含量分别是第4、第3和第1序位最高。相关性分析表明,3个品种的单果重均与果实横径存在显著的相关性,其中翠玉的单果重与果实横径的关系更为密切。综合分析结果:设施促成栽培翠冠、翠玉的留果序位分别以第4、第3序位为好,新玉则初步建议为第4序位较好。本研究结果可以为南方早熟梨的设施促成栽培提供参考。 展开更多
关键词 设施栽培 花序序位 单果重 果实纵径和横径 果柄 可溶性固形物
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负压灌溉对玉米磷吸收和根际土壤磷含量的影响
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作者 张吉立 刘振平 +2 位作者 王鹏 龙怀玉 高明菊 《植物营养与肥料学报》 北大核心 2025年第12期2483-2493,共11页
[目的] 探究负压灌溉对玉米各器官磷吸收、磷含量和根际土磷素变化的影响规律,为提高负压供水利用效率提供理论依据。[方法] 2019和2020年,在黑龙江八一农垦大学试验基地防雨棚内开展负压灌溉盆栽试验,供试玉米品种为‘先玉335’。试验... [目的] 探究负压灌溉对玉米各器官磷吸收、磷含量和根际土磷素变化的影响规律,为提高负压供水利用效率提供理论依据。[方法] 2019和2020年,在黑龙江八一农垦大学试验基地防雨棚内开展负压灌溉盆栽试验,供试玉米品种为‘先玉335’。试验设置负压灌溉-5 kPa、-10 kPa、-15 kPa 3个供水压力,以常规浇灌为对照(CK)。于成熟期测定玉米株高、茎粗、产量,分析不同生育时期各器官磷吸收量及磷含量变化,并监测玉米根际土全磷、有效磷含量变化。[结果] 与CK相比,-5 kPa处理在2019年和2020年分别显著提高玉米产量38.7%、16.2%,而-15 kPa处理产量显著低于CK和-5 kPa处理。成熟期时,-5 kPa处理下,根、茎、叶、穗轴、籽粒及整株的磷吸收量在2019年分别较CK显著提高120.8%、86.6%、26.4%、66.9%、15.2%、43.3%,2020年分别显著提高15.4%、22.4%、47.0%、29.7%、30.0%、21.3%。-10 kPa处理下,根、茎、叶磷吸收量在2019年分别较CK提高47.1%、53.0%、10.7%,2020年叶片磷吸收量提高10.1%,其余器官磷吸收量均低于CK。-15 kPa处理下,各器官及磷吸收量均显著低于CK和-10 kPa处理。2019年,-5 kPa处理下,根系、茎、叶、苞叶、穗轴的磷含量分别较CK提高22.4%、71.8%、10.1%、10.0%、43.7%;-10 kPa处理下,茎、叶、苞叶、穗轴、籽粒的磷含量分别较CK提高102.8%、17.9%、13.8%、19.8%、11.5%;-15 kPa处理下,茎、叶、苞叶、穗轴的磷含量分别较CK提高136.9%、38.9%、98.2%、66.1%。2020年,-5 kPa处理下,根系、茎、叶、穗轴的磷含量分别较CK提高34.1%、17.2%、13.6%、28.4%;-10 kPa处理下,根系、叶、穗轴的磷含量分别较CK提高30.5%、27.6%、22.6%。与CK相比,-5 kPa处理下,磷偏生产力、磷效率在2019年分别提高38.7%、41.7%,2020年分别提高16.2%、18.8%。抽雄期和成熟期,-5 kPa处理下的土壤全磷含量较CK在2019年分别降低8.1%和10.9%,2020年分别降低5.4%和6.0%;-10 kPa处理与-5 kPa处理间无显著差异,-15 kPa处理与CK间也无显著差异。同样在抽雄期和成熟期,-5 kPa处理下的土壤有效磷含量较CK在2019年分别降低17.6%和36.0%,2020年分别降低19.2%和21.9%;-10 kPa处理与-5 kPa处理间无显著差异。2019年,-15 kPa处理下根际土有效磷含量显著低于CK和-5 kPa处理;2020年成熟期,-15 kPa处理下根际土壤有效磷含量显著低于CK。[结论] 负压灌溉在-5 kPa供水压力下促进玉米生长、提高产量和磷吸收效果优于CK、-10 kPa和-15 kPa处理,同时降低根际土全磷和有效磷含量。 展开更多
关键词 玉米 株高 茎粗 磷吸收 根际土磷含量
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干细胞在小口径人工血管内皮化中的应用
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作者 郑安垲 刘瑞明 向秋玲 《中国组织工程研究》 CAS 北大核心 2025年第1期120-127,共8页
背景:小口径人工血管移植后由于血栓形成和内膜增生等原因造成管腔狭窄甚至闭塞,运用干细胞作为种子细胞实现人工血管的内皮化有助于改善血管移植后的远期通畅率。目的:总结干细胞在小口径人工血管内皮化中应用的研究进展。方法:由第一... 背景:小口径人工血管移植后由于血栓形成和内膜增生等原因造成管腔狭窄甚至闭塞,运用干细胞作为种子细胞实现人工血管的内皮化有助于改善血管移植后的远期通畅率。目的:总结干细胞在小口径人工血管内皮化中应用的研究进展。方法:由第一作者检索Pub Med和万方数据库2013-2023年发表的相关文献,英文检索词为“vascular graft,tissue-engineered blood vessel/vascular tissue engineering,endothelialization,stem cells,endothelial progenitor cells,mesenchymal stem cells,induced pluripotent stem cells,embryonicstemcells”,中文检索词为“人工血管,组织工程血管/血管组织工程,内皮化,干细胞,内皮祖细胞,间充质干细胞,诱导多能干细胞,胚胎干细胞”,检索近10年国内外关于干细胞应用于小口径人工血管内皮化的相关文献,初检文献552篇,根据纳排标准最终选取81篇文献进行综述。结果与结论:(1)远期通畅率不理想限制了小口径人工血管的临床应用,造成远期通畅率低下的主要原因是血栓形成和内膜增生。天然血管内皮层具有良好的抗血栓及内膜增生性能,内皮化可以模拟天然血管的特性,是提升远期通畅率的有效手段。(2)小口径人工血管植入体内后会经历体内内皮化过程,但难以形成完整的内皮层。干细胞具有分化为内皮细胞的潜能,体内招募干细胞或将其作为种子细胞种植在人工血管内表面是实现内皮化的研究策略。(3)将内皮祖细胞、间充质干细胞、诱导多能干细胞及胚胎干细胞等作为种子细胞种植均能够一定程度改善小口径人工血管的远期通畅率,且它们各具优势。内皮祖细胞便于获取且可直接用于种植;间充质干细胞来源广泛且具有旁分泌和调节免疫的功能;诱导多能干细胞来源丰富且可消除免疫原性;胚胎干细胞增殖能力强且能多向分化。(4)将干细胞用于人工血管的研究目前仍未转化至临床,未来需要进一步研究并促进临床转化。 展开更多
关键词 小口径人工血管 内皮化 干细胞 内皮祖细胞 间充质干细胞 诱导多能干细胞 胚胎干细胞 综述
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薄皮甜瓜茎粗和节间长性状主基因+多基因遗传分析
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作者 陈英达 孙浩 +6 位作者 于文慧 刘瑞梅 荣维瑞 韩靖漪 潘玉朋 刘振宁 张宁 《江西农业大学学报》 北大核心 2025年第4期922-931,共10页
【目的】旨在探究薄皮甜瓜茎粗和节间长性状的遗传基础,为甜瓜茎粗和节间长性状的遗传改良提供理论指导,为培育甜瓜理想株型品种提供理论依据。【方法】以薄皮甜瓜高代自交系M125和M30为试验材料,构建六世代群体,利用遗传模型分析软件... 【目的】旨在探究薄皮甜瓜茎粗和节间长性状的遗传基础,为甜瓜茎粗和节间长性状的遗传改良提供理论指导,为培育甜瓜理想株型品种提供理论依据。【方法】以薄皮甜瓜高代自交系M125和M30为试验材料,构建六世代群体,利用遗传模型分析软件包对茎粗及节间长进行遗传模型的分析,并计算24种模型的极大似然函数值(MLV)和赤池信息量(AIC),采用AIC最小原则进行模型初选,各性状分别选择3个模型作为备选模型。对备选模型进行适合性检验,选取差异显著量最少的备选模型作为最优模型,并计算出最优模型的一阶和二阶遗传参数。【结果】统计分析表明,F_(1)植株茎粗及节间长均值均介于两亲本之间,且明显偏向于母本。由F2群体植株茎粗及节间长频次分布可知,2个性状均呈现连续变异,且为偏态分布。薄皮甜瓜植株茎粗符合2对加性-显性-上位性主基因+加性-显性多基因遗传模型,其一阶遗传参数表明,2对主基因的加性效应值均为1.51,第1对主基因的显性效应值为-0.39,第2对主基因的显性效应值为0.06,2对主基因的显性度(|h_(a)/d_(a)|、|h_(b)/d_(b)|)分别为0.26和0.04,表明2对主基因呈部分显性作用;2对主基因加性效应绝对值之和大于显性效应绝对值之和,表明甜瓜茎粗性状的遗传以加性正向效应为主,并具有增效作用。二阶遗传参数表明,F2群体的主基因遗传率为82.27%,多基因遗传率为3.21%,B_(1)和B_(2)的主基因遗传率分别为83.75%和52.13%,多基因遗传率分别为7.27%和0。节间长符合2对加性-显性-上位性主基因+加性-显性-上位性多基因遗传模型,其一阶遗传参数表明,2对主基因的加性效应值均为13.26,第1对主基因的显性效应值为-17.17,第2对主基因的显性效应值为4.38,2对主基因的显性度分别为1.29和0.33,表明第1对主基因呈超显性作用;第2对主基因呈部分显性作用,2对主基因加性效应绝对值之和大于显性效应绝对值之和,表明甜瓜茎节间长性状的遗传以加性正向效应为主,且加性效应强于显性效应。二阶遗传参数表明,F2群体的主基因遗传率为84.26%,多基因遗传率为8.93%,B_(1)和B_(2)的主基因遗传率分别为77.76%和89.99%,多基因遗传率分别为14.58%和0。【结论】薄皮甜瓜植株茎粗和节间长性状的主基因遗传率较高,且主基因的加性效应均强于显性效应。因此,甜瓜育种过程中对茎粗和节间长性状的选择适宜在早期世代进行。 展开更多
关键词 薄皮甜瓜 茎粗 节间长 数量性状 主基因+多基因 遗传分析
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基于主成分分析的铁皮石斛主要农艺性状评价研究 被引量:1
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作者 周慧君 谢宇 +4 位作者 曾凡 刘艺 陈芳兰 魏翠华 陈沁 《东南园艺》 2025年第1期64-69,共6页
【目的】为了科学评价铁皮石斛不同种质的农艺性状,筛选出适宜福建福州地区塑料大棚种植的铁皮石斛优良种质。【方法】采用主成分分析、相关性分析对收集的10份铁皮石斛种质进行了主要农艺性状的综合评价。【结果】不同铁皮石斛种质的... 【目的】为了科学评价铁皮石斛不同种质的农艺性状,筛选出适宜福建福州地区塑料大棚种植的铁皮石斛优良种质。【方法】采用主成分分析、相关性分析对收集的10份铁皮石斛种质进行了主要农艺性状的综合评价。【结果】不同铁皮石斛种质的农艺性状间存在差异,其中浙江乐清的种质(TZ1)的茎长最长(33.32cm)、茎直径为5.33mm、叶面积最大,为11.1cm^(2);各性状间存在一定的相关性,茎长与节数、叶数、叶宽、叶面积呈极显著正相关,而与茎直径、叶形指数呈负相关;茎直径和节直径之间为极显著正相关,均与节数呈极显著负相关,与叶长呈显著负相关;叶面积与叶长、叶宽呈极显著正相关;根据主成分贡献率进行综合评价,得分排名前3的种质分别为浙江乐清的种质(TZ1)、浙江临安的种质(TZ2)、安徽霍山的种质(TA1),具有茎长较长且节数较多,叶片数量多且叶片较大的特征。【结论】综合茎直径比较,浙江乐清的种质(TZ1)的农艺性状更优秀,适合在福州大棚内种植。 展开更多
关键词 铁皮石斛 相关性分析 主成分分析 农艺性状 综合评价 种质 茎直径
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不同基质配比对何首乌组培苗生长情况的影响
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作者 卢娟 李莉莉 +5 位作者 叶鸿飞 孙熹 尹玉军 王海雁 陆栩雅 张烨 《广西农学报》 2025年第3期17-22,31,共7页
为了解决何首乌规模化种植的育苗技术问题,文章采用在10种不同配方基质培育何首乌组培苗,通过测定其移栽存活率、苗高、地径、叶片含氮量及块根大小等指标,以期寻找出适合何首乌组培苗生长的最佳基质配方。试验结果显示:何首乌组培苗在... 为了解决何首乌规模化种植的育苗技术问题,文章采用在10种不同配方基质培育何首乌组培苗,通过测定其移栽存活率、苗高、地径、叶片含氮量及块根大小等指标,以期寻找出适合何首乌组培苗生长的最佳基质配方。试验结果显示:何首乌组培苗在移栽到不同基质50 d后,各生长指标均有所不同。其中,以纯黄心土为基质时成活率仅为50%,而以泥炭土为基质时存活率最高,为90%,且苗高最高可达92 cm,地径最大为2.706 mm,含氮量平均为15.9 mg/g。在移栽到不同基质培育180 d后,以泥炭土为基质时块根平均值最大,宽度为6.05 mm,长度为42.85 mm。综上所述,在椰糠、泥炭土、黄心土3种育苗基质及其不同配比中,育苗基质仅是泥炭土时,何首乌组培苗各项生长指标均最佳,最有利于组培苗的生长。 展开更多
关键词 何首乌 组培苗 轻基质 移栽存活率 苗高 地径
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