Recently,Mueller matrix(MM)polarimetric imaging-assisted pathology detection methods are showing great potential in clinical diagnosis.However,since our human eyes cannot observe polarized light directly,it raises a n...Recently,Mueller matrix(MM)polarimetric imaging-assisted pathology detection methods are showing great potential in clinical diagnosis.However,since our human eyes cannot observe polarized light directly,it raises a notable challenge for interpreting the measurement results by pathologists who have limited familiarity with polarization images.One feasible approach is to combine MM polarimetric imaging with virtual staining techniques to generate standardized stained images,inheriting the advantages of information-abundant MM polarimetric imaging.In this study,we develop a model using unpaired MM polarimetric images and bright-field images for generating standard hematoxylin and eosin(H&E)stained tissue images.Compared with the existing polarization virtual staining techniques primarily based on the model training with paired images,the proposed Cycle-Consistent Generative Adversarial Networks(CycleGAN)-based model simplifies data acquisition and data preprocessing to a great extent.The outcomes demonstrate the feasibility of training CycleGAN with unpaired polarization images and their corresponding bright-field images as a viable approach,which provides an intuitive manner for pathologists for future polarization-assisted digital pathology.展开更多
目的系统评估瑞氏–吉姆萨染色在阴道炎综合诊断中的应用潜力及诊断效能。方法选取2025年1月至3月于合肥医科大学附属医院就诊的1222例患者为研究对象,采集阴道分泌物样本,分别进行湿片显微镜检查、瑞氏–吉姆萨染色显微镜检查、革兰染...目的系统评估瑞氏–吉姆萨染色在阴道炎综合诊断中的应用潜力及诊断效能。方法选取2025年1月至3月于合肥医科大学附属医院就诊的1222例患者为研究对象,采集阴道分泌物样本,分别进行湿片显微镜检查、瑞氏–吉姆萨染色显微镜检查、革兰染色显微镜检查,并检测pH、白细胞酯酶、唾液酸苷酶等功能学指标。分析三种显微镜检查方法检测白细胞(white blood cell,WBC)评分、基底旁细胞(parabasal cell,PBC)评分、需氧菌性阴道病(aerobic vaginitis,AV)背景菌评分、乳酸杆菌等级(Lactobacillus grading,LBG)、LBG评分、乳酸杆菌丰度评分、动弯杆菌评分、加德纳菌评分、真菌、滴虫和线索细胞的差异和一致性。采用Spearman相关性检验分析WBC评分、LBG、加德纳菌评分与其对应功能学指标的相关性。计算敏感度、特异性、曲线下面积(area under the curve,AUC)等指标评估瑞氏–吉姆萨染色的诊断效能。结果瑞氏–吉姆萨染色对LBG、PBC评分、AV背景菌评分、WBC评分、加德纳菌评分、动弯杆菌评分的等级判断显著高于革兰染色(P<0.0167),对加德纳菌评分的等级判断显著高于湿片法(P<0.0167),且湿片法未检出动弯杆菌。Spearman相关性检验结果显示,瑞氏–吉姆萨染色的加德纳菌评分与唾液酸苷酶、LBG与pH的相关性最好(r=0.6922、0.7861)。McNemar检验结果显示瑞氏–吉姆萨染色对真菌的检出率显著高于湿片法,对滴虫的检出率显著高于革兰染色,对线索细胞的检出率显著高于湿片法和革兰染色(P<0.0167)。观察者间一致性分析结果显示,除线索细胞(部分观察者Kappa=0.390)外,其余指标(如PBC评分、LBG、真菌、滴虫)均表现为中高度一致性。瑞氏–吉姆萨染色诊断外阴阴道假丝酵母菌病、滴虫性阴道炎、细菌性阴道病和细胞溶解性阴道病的敏感度和特异性均在90%以上。结论瑞氏–吉姆萨染色在阴道炎诊断中兼具高检出率、高一致性、低成本、易操作的优势,有望成为阴道炎综合诊断的常规方法,尤其在医疗资源有限地区具有重要推广价值。展开更多
One of the significant features of The Human Stain is that it lays bare the reality of American society and censures the Anglo-Saxon or white discourse.What are equally important are the multiple narrative strategies ...One of the significant features of The Human Stain is that it lays bare the reality of American society and censures the Anglo-Saxon or white discourse.What are equally important are the multiple narrative strategies that Philip R oth employs in the novel.T his paper focuses mainly on the elaboration of such narrative strategies as mimesis and diegesis,complicated narrative time,shifting focalizations,and narration or non-narrative comments,so as to point out that these narrative strategies add colors to its( postmodern) artistic features,becoming an integral component of this novel.展开更多
Selecting oocytes that are most likely to develop is crucial for in vitro fertilization and animal cloning. Brilliant cresyl blue （BCB） staining has been used for oocyte selection in large animals, but its wider uti...Selecting oocytes that are most likely to develop is crucial for in vitro fertilization and animal cloning. Brilliant cresyl blue （BCB） staining has been used for oocyte selection in large animals, but its wider utility needs further evaluation. Mouse oocytes were divided into those stained （BCB＋） and those unstained （BCB-） according to their ooplasm BCB coloration. Chromatin configurations, cumulus cell apoptosis, cytoplasmic maturity and developmental competence were compared between the BCB＋ and BCB- oocytes. The effects of oocyte diameter, sexual maturity and gonadotropin stimulation on the competence of BCB＋ oocytes were also analyzed. In the large- and medium-size groups, BCB＋ oocytes were larger and showed more surrounded nucleoli （SN） chromatin configurations and higher frequencies of early atresia, and they also gained better cytoplasmic maturity （determined as the intracellular GSH level and pattern of mitochondrial distribution） and higher developmental potential after in vitro maturation （IVM） than the BCB-oocytes. Adult mice produced more BCB＋ oocytes with higher competence than the prepubertal mice when not primed with PMSG. PMSG priming increased both proportion and developmental potency of BCB＋ oocytes. The BCB＋ oocytes in the large-size group showed more SN chromatin configurations, better cytoplasmic maturity and higher developmental potential than their counterparts in the medium-size group. It is concluded that BCB staining can be used as an efficient method for oocyte selection, but that the competence of the BCB＋ oocytes may vary with oocyte diameter, animal sexual maturity and gonadotropin stimulation. Taken together, the series of criteria described here would allow for better choices in selecting oocytes for better development.展开更多
AIM: To explore an efficient, practical and objective quantitative method to evaluate the retinal neovascularization in mouse model of oxygen induced retinopathy (OIR). METHODS: Thirty C57BL/6J mice were explored in O...AIM: To explore an efficient, practical and objective quantitative method to evaluate the retinal neovascularization in mouse model of oxygen induced retinopathy (OIR). METHODS: Thirty C57BL/6J mice were explored in OIR model procedure. Eyes were removed for different staining methods including: (1) HE staining; (2) immunohistochemistry with Griffonia Simplicifolia Lectin (GSL); (3) Immunofluorescence with FITC labeled CD31 antibody; (4) Two-step immunofluorescence with purified-CD31 antibody; (5) FITC-Dextran perfusion combined with two-step purified-CD31immunofluorescence. Images of the retinal vasculature were analyzed by imaging software. ' RESULTS: GSL immunohistochemistry could clearly demonstrate the deep and superficial capillary beds. FITC labeled CD31 Immunofluorescence was blurring with high fluorescence background which was hard to distinguish retinal neovascularization in some area. Excellent detail of neovascularization and preexistent retinal vessels was provided in two-step Purified-CD31 immunofluorescence group. CONCLUSION: GSL immunohistochemistry can clearly demonstrate neovascularization tufts in deep and superficial capillary beds. Immunofluorescence of specific antigen CD31 on vascular endothelium can selectively label the neovascularization of mouse retina. When combined with computer analysis software, it is an effective and objective quantitative method to evaluate the retinal neovascularization in OIR mouse model.展开更多
AIM： To detect Salmonella enteritidis （S. enteritidis） in paraffin slices and antigen location in infected duck tissues. METHODS： The rabbits were immunized with purified bacillus to obtain S. enteritidis-specific...AIM： To detect Salmonella enteritidis （S. enteritidis） in paraffin slices and antigen location in infected duck tissues. METHODS： The rabbits were immunized with purified bacillus to obtain S. enteritidis-specific antibody, which were then extracted by the caprylic-ammonium sulphate method, purified through High-Q columns. An indirect immuno-fluorescent staining method （IFA） was established to detect the S. enteritidis antigen in paraffin slices. Detected S. enteritidis in each organ tissue of ducklings experimentally infected with S. enteritidis. RESULTS： The gland of Garder, heart, kidney, spleen, liver, brain, ileum, jejunum, bursa of Fabricius from S. enteritidis experimentally infected ducklings were positive or strongly positive, and the S. enteritidis antigen mainly distributed in the infected cell cytoplasm.CONCLUSION： IFA is an intuitionist, sensitive and specific method in detecting S. enteritidis antigen in paraffin wax slices, and it is a good method in diagnosis and antigen location of S. enteritidis. We also conclude that the gland of Garder, heart, kidney, spleen, liver, ileum, jejunum are target organs in S. enteritidis infections of duck, and S. enteritidis is an intracellular parasitic bacterium.展开更多
基金Shenzhen Key Fundamental Research Project(No.JCYJ20210324120012035).
文摘Recently,Mueller matrix(MM)polarimetric imaging-assisted pathology detection methods are showing great potential in clinical diagnosis.However,since our human eyes cannot observe polarized light directly,it raises a notable challenge for interpreting the measurement results by pathologists who have limited familiarity with polarization images.One feasible approach is to combine MM polarimetric imaging with virtual staining techniques to generate standardized stained images,inheriting the advantages of information-abundant MM polarimetric imaging.In this study,we develop a model using unpaired MM polarimetric images and bright-field images for generating standard hematoxylin and eosin(H&E)stained tissue images.Compared with the existing polarization virtual staining techniques primarily based on the model training with paired images,the proposed Cycle-Consistent Generative Adversarial Networks(CycleGAN)-based model simplifies data acquisition and data preprocessing to a great extent.The outcomes demonstrate the feasibility of training CycleGAN with unpaired polarization images and their corresponding bright-field images as a viable approach,which provides an intuitive manner for pathologists for future polarization-assisted digital pathology.
文摘目的系统评估瑞氏–吉姆萨染色在阴道炎综合诊断中的应用潜力及诊断效能。方法选取2025年1月至3月于合肥医科大学附属医院就诊的1222例患者为研究对象,采集阴道分泌物样本,分别进行湿片显微镜检查、瑞氏–吉姆萨染色显微镜检查、革兰染色显微镜检查,并检测pH、白细胞酯酶、唾液酸苷酶等功能学指标。分析三种显微镜检查方法检测白细胞(white blood cell,WBC)评分、基底旁细胞(parabasal cell,PBC)评分、需氧菌性阴道病(aerobic vaginitis,AV)背景菌评分、乳酸杆菌等级(Lactobacillus grading,LBG)、LBG评分、乳酸杆菌丰度评分、动弯杆菌评分、加德纳菌评分、真菌、滴虫和线索细胞的差异和一致性。采用Spearman相关性检验分析WBC评分、LBG、加德纳菌评分与其对应功能学指标的相关性。计算敏感度、特异性、曲线下面积(area under the curve,AUC)等指标评估瑞氏–吉姆萨染色的诊断效能。结果瑞氏–吉姆萨染色对LBG、PBC评分、AV背景菌评分、WBC评分、加德纳菌评分、动弯杆菌评分的等级判断显著高于革兰染色(P<0.0167),对加德纳菌评分的等级判断显著高于湿片法(P<0.0167),且湿片法未检出动弯杆菌。Spearman相关性检验结果显示,瑞氏–吉姆萨染色的加德纳菌评分与唾液酸苷酶、LBG与pH的相关性最好(r=0.6922、0.7861)。McNemar检验结果显示瑞氏–吉姆萨染色对真菌的检出率显著高于湿片法,对滴虫的检出率显著高于革兰染色,对线索细胞的检出率显著高于湿片法和革兰染色(P<0.0167)。观察者间一致性分析结果显示,除线索细胞(部分观察者Kappa=0.390)外,其余指标(如PBC评分、LBG、真菌、滴虫)均表现为中高度一致性。瑞氏–吉姆萨染色诊断外阴阴道假丝酵母菌病、滴虫性阴道炎、细菌性阴道病和细胞溶解性阴道病的敏感度和特异性均在90%以上。结论瑞氏–吉姆萨染色在阴道炎诊断中兼具高检出率、高一致性、低成本、易操作的优势,有望成为阴道炎综合诊断的常规方法,尤其在医疗资源有限地区具有重要推广价值。
文摘One of the significant features of The Human Stain is that it lays bare the reality of American society and censures the Anglo-Saxon or white discourse.What are equally important are the multiple narrative strategies that Philip R oth employs in the novel.T his paper focuses mainly on the elaboration of such narrative strategies as mimesis and diegesis,complicated narrative time,shifting focalizations,and narration or non-narrative comments,so as to point out that these narrative strategies add colors to its( postmodern) artistic features,becoming an integral component of this novel.
基金Acknowledgments This study was supported by grants from the China National Natural Science Foundation （Nos. 30430530 and 30571337） and from the Momentous Research Project of the China Ministry of Science and Technology （No. 2006CB944003）.
文摘Selecting oocytes that are most likely to develop is crucial for in vitro fertilization and animal cloning. Brilliant cresyl blue （BCB） staining has been used for oocyte selection in large animals, but its wider utility needs further evaluation. Mouse oocytes were divided into those stained （BCB＋） and those unstained （BCB-） according to their ooplasm BCB coloration. Chromatin configurations, cumulus cell apoptosis, cytoplasmic maturity and developmental competence were compared between the BCB＋ and BCB- oocytes. The effects of oocyte diameter, sexual maturity and gonadotropin stimulation on the competence of BCB＋ oocytes were also analyzed. In the large- and medium-size groups, BCB＋ oocytes were larger and showed more surrounded nucleoli （SN） chromatin configurations and higher frequencies of early atresia, and they also gained better cytoplasmic maturity （determined as the intracellular GSH level and pattern of mitochondrial distribution） and higher developmental potential after in vitro maturation （IVM） than the BCB-oocytes. Adult mice produced more BCB＋ oocytes with higher competence than the prepubertal mice when not primed with PMSG. PMSG priming increased both proportion and developmental potency of BCB＋ oocytes. The BCB＋ oocytes in the large-size group showed more SN chromatin configurations, better cytoplasmic maturity and higher developmental potential than their counterparts in the medium-size group. It is concluded that BCB staining can be used as an efficient method for oocyte selection, but that the competence of the BCB＋ oocytes may vary with oocyte diameter, animal sexual maturity and gonadotropin stimulation. Taken together, the series of criteria described here would allow for better choices in selecting oocytes for better development.
基金Supported by National Natural Science Foundation of China(No.30973899)
文摘AIM: To explore an efficient, practical and objective quantitative method to evaluate the retinal neovascularization in mouse model of oxygen induced retinopathy (OIR). METHODS: Thirty C57BL/6J mice were explored in OIR model procedure. Eyes were removed for different staining methods including: (1) HE staining; (2) immunohistochemistry with Griffonia Simplicifolia Lectin (GSL); (3) Immunofluorescence with FITC labeled CD31 antibody; (4) Two-step immunofluorescence with purified-CD31 antibody; (5) FITC-Dextran perfusion combined with two-step purified-CD31immunofluorescence. Images of the retinal vasculature were analyzed by imaging software. ' RESULTS: GSL immunohistochemistry could clearly demonstrate the deep and superficial capillary beds. FITC labeled CD31 Immunofluorescence was blurring with high fluorescence background which was hard to distinguish retinal neovascularization in some area. Excellent detail of neovascularization and preexistent retinal vessels was provided in two-step Purified-CD31 immunofluorescence group. CONCLUSION: GSL immunohistochemistry can clearly demonstrate neovascularization tufts in deep and superficial capillary beds. Immunofluorescence of specific antigen CD31 on vascular endothelium can selectively label the neovascularization of mouse retina. When combined with computer analysis software, it is an effective and objective quantitative method to evaluate the retinal neovascularization in OIR mouse model.
基金the National Key Technology R&D Program of China, No. 2004BA 901A 03National Scientific and Sechnical Support Program, No. 2007Z06-017+3 种基金The Cultvation Fund of the Key Scientific and Technical Innovation Project & Ministry of Education of China, No. 706050Program for New Century Excellent Talents in University, No. NCET-04-0906/NCET-06-0818Sichuan Province Basic Research Program, No. 04JY0290061/07JY029-017Program for Key Disciplines Construction of Sichuan Province No. SZD0418
文摘AIM： To detect Salmonella enteritidis （S. enteritidis） in paraffin slices and antigen location in infected duck tissues. METHODS： The rabbits were immunized with purified bacillus to obtain S. enteritidis-specific antibody, which were then extracted by the caprylic-ammonium sulphate method, purified through High-Q columns. An indirect immuno-fluorescent staining method （IFA） was established to detect the S. enteritidis antigen in paraffin slices. Detected S. enteritidis in each organ tissue of ducklings experimentally infected with S. enteritidis. RESULTS： The gland of Garder, heart, kidney, spleen, liver, brain, ileum, jejunum, bursa of Fabricius from S. enteritidis experimentally infected ducklings were positive or strongly positive, and the S. enteritidis antigen mainly distributed in the infected cell cytoplasm.CONCLUSION： IFA is an intuitionist, sensitive and specific method in detecting S. enteritidis antigen in paraffin wax slices, and it is a good method in diagnosis and antigen location of S. enteritidis. We also conclude that the gland of Garder, heart, kidney, spleen, liver, ileum, jejunum are target organs in S. enteritidis infections of duck, and S. enteritidis is an intracellular parasitic bacterium.
