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AZ31 magnesium alloy sheet with high ductility and low anisotropy achieved by a novel asymmetrical angular rolling process
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作者 Chenze Wang Zan Liu +3 位作者 Zhihui Cai Lifang Pan Guangming Liu Lifeng Ma 《Journal of Magnesium and Alloys》 2025年第9期4561-4576,共16页
The low ductility and strong mechanical anisotropy of wrought magnesium alloys have hindered their further processing and application.In this study,AZ31 magnesium alloy sheet was prepared by a new asymmetrical angular... The low ductility and strong mechanical anisotropy of wrought magnesium alloys have hindered their further processing and application.In this study,AZ31 magnesium alloy sheet was prepared by a new asymmetrical angular rolling(AAR)process,compared with conventional symmetrical rolling(SR)process and asymmetrical rolling(ASR)process.The effects of three rolling processes on the microstructure,texture and mechanical properties of the alloy sheets were systematically studied.The results show that the AAR sheet exhibits excellent mechanical properties compared to other two rolling processes.It not only achieves the highest ductility of 17.9%,17.9%,and 18.5% in the three directions,but also has the lowest mechanical anisotropy values for yield strength,ultimate tensile strength and elongation.The AAR process significantly reduces the anisotropy of the material by achieving the smallest average grain size of 4.93μm and the most homogeneous grain size distribution.Introduced bi-directional asymmetric shear stresses randomizes grain orientation and activates the non-basal slip system,which also significantly reduces the anisotropy.In addition,the tensile twinning mechanism dominates during the AAR process,which contributes to texture weakening and the activation of the non-basal slip system.Through the synergy of these mechanisms,the AAR sheet is characterized by high ductility and low anisotropy. 展开更多
关键词 Asymmetrical angular rolling TWINNING Texture evolution Mechanical anisotropy
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Precision flatness measurement based on orbital angular momentum
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作者 Feifei Han Zhiwan Wang +1 位作者 Le Wang Shengmei Zhao 《Chinese Physics B》 2025年第5期415-422,共8页
We propose a method to measure the flatness of an object with a petal-like pattern generated by the interference of the measured orbital angular momentum(OAM)beam and the reference OAM beam which carries the opposite ... We propose a method to measure the flatness of an object with a petal-like pattern generated by the interference of the measured orbital angular momentum(OAM)beam and the reference OAM beam which carries the opposite OAM state.By calculating the difference between the petal rotation angle without/with the object,the thickness information of the object,and then the flatness information,can be evaluated.Furthermore,the direction of the object’s flatness can be determined by the petal’s clockwise/counterclockwise rotation.We theoretically analyze the relationship between the object’s thickness and petal rotation angle,and verify the proposed method by experiment.The experimental results show that the proposed method is a high precision flatness measurement and can obtain the convex/concave property of the flatness.For the 1.02 mm glass sample,the mean deviation of the flatness is 1.357×10^(-8) and the variance is 0.242×10^(-16).For the 0.50 mm glass sample,the mean deviation of the flatness is 1.931×10^(-8) and the variance is 2.405×10^(-16).Two different topological charges are adopted for the 2.00 mm glass sample,and their flatness deviations are 0.239×10^(-8)(ℓ=1)and 0.246×10^(-8)(ℓ=2),where their variances are 0.799×10^(-18)(ℓ=1)and 0.775×10^(-18)(ℓ=2),respectively.It is shown that the flatness measured by the proposed method is the same for the same sample when different topological charges are used.All results indicate that the proposed method may provide a high flatness measurement,and will be a promising way to measure the flatness. 展开更多
关键词 orbital angular momentum flatness measurement INTERFERENCE petal rotation angle
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Mask-coding-assisted continuous-variable quantum direct communication with orbital angular momentum multiplexing
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作者 Zhengwen Cao Yujie Wang +2 位作者 Geng Chai Xinlei Chen Yuan Lu 《Chinese Physics B》 2025年第2期64-72,共9页
Quantum secure direct communication(QSDC) is a communication method based on quantum mechanics and it is used to transmit secret messages. Unlike quantum key distribution, secret messages can be transmitted directly o... Quantum secure direct communication(QSDC) is a communication method based on quantum mechanics and it is used to transmit secret messages. Unlike quantum key distribution, secret messages can be transmitted directly on a quantum channel with QSDC. Higher channel capacity and noise suppression capabilities are key to achieving longdistance quantum communication. Here, we report a continuous-variable QSDC scheme based on mask-coding and orbital angular momentum, in which the mask-coding is employed to protect the security of the transmitting messages and to suppress the influence of excess noise. The combination of orbital angular momentum and information block transmission effectively improves the secrecy capacity. In the 800 information blocks ×1310 bits length 10-km experiment, the results show a statistical average bit error rate of 0.38%, a system excess noise value of 0.0184 SNU, and a final secrecy capacity of 6.319×10~6 bps. Therefore, this scheme reduces error bits while increasing secrecy capacity, providing a solution for long-distance large-scale quantum communication, which is capable of transmitting text, images and other information of reasonable size. 展开更多
关键词 continuous-variable quantum direct communication orbital angular momentum mask coding
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Mechanical properties and microstructure of as-cast AA 7050 processed by equal channel angular pressing combined with inter-pass aging treatment
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作者 LI Jian HE Tao +2 位作者 DU Xiang-yang JIA Dong-sheng VERESCHAKA Alexy 《Journal of Central South University》 2025年第5期1678-1696,共19页
In this study,the interaction between deformation and precipitates during multiple equal channel angular pressing(ECAP)deformations and inter-pass aging combination and its effect on the mechanical properties of 7050 ... In this study,the interaction between deformation and precipitates during multiple equal channel angular pressing(ECAP)deformations and inter-pass aging combination and its effect on the mechanical properties of 7050 aluminum alloy are studied.The result show that ECAP induces numerous substructures and dislocations,effectively promoting the precipitation of theηʹphase exhibiting a bimodal structure during inter-pass aging.Following inter-pass aging and subsequent ECAP,the decrease in grain size(4.8μm)is together with the increase in dislocation density(1.24×10^(15) m^(−2))due to the pinning effect of the precipitated phase.Simultaneously,the dislocation motion causes the second phase particles to become even finer and more diffuse.The synergistic effects of precipitation strengthening,fine grain strengthening,and dislocation strengthening collectively enhance the high strength of aluminum alloys,with ultimate tensile strength and yield strength reaching approximately 610 and 565 MPa,respectively.Meanwhile,ductility remains largely unchanged,primarily due to coordinated grain boundary sliding and the uniform and fine dispersion of second phase particles. 展开更多
关键词 equal channel angular pressing 7050 aluminum alloys inter-pass aging mechanical properties PRECIPITATES dislocation
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Spatially resolved spin angular momentum mediated by spin–orbit interaction in tightly focused spinless vector beams in optical tweezers
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作者 Ram Nandan Kumar Sauvik Roy +2 位作者 Subhasish Dutta Gupta Nirmalya Ghosh Ayan Banerjee 《Advanced Photonics Nexus》 2025年第2期54-63,共10页
We demonstrate an effective and optimal strategy for generating spatially resolved longitudinal spin angular momentum(LSAM)in optical tweezers by tightly focusing the first-order spirally polarized vector(SPV)beams wi... We demonstrate an effective and optimal strategy for generating spatially resolved longitudinal spin angular momentum(LSAM)in optical tweezers by tightly focusing the first-order spirally polarized vector(SPV)beams with zero intrinsic angular momentum into a refractive index stratified medium.The stratified medium gives rise to a spherically aberrated intensity profile near the focal region of the optical tweezers,with off-axis intensity lobes in the radial direction possessing opposite LSAM(helicities corresponding toσ=+1 and−1)compared to the beam center.We trap mesoscopic birefringent particles in an off-axis intensity lobe as well as at the beam center by modifying the trapping plane and observe particles spinning in opposite directions depending on their location.The direction of rotation depends on the particle size with larger particles spinning either clockwise or anticlockwise depending on the direction of spirality of the polarization of the SPV beam after tight focusing,while smaller particles spin in both directions depending on their spatial locations.Numerical simulations support our experimental observations.Our results introduce new avenues in spin-orbit optomechanics to facilitate novel yet straightforward avenues for exotic and complex particle manipulation in optical tweezers. 展开更多
关键词 spin angular momentum optical tweezer structured vector beam spin-orbit interaction refractive index-stratified medium birefringent liquid crystal
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Synchronous enhancement of corrosion resistance and mechanical properties of Mg-Zn-Ca alloys by grain refinement using equal channel angular pressing
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作者 Yi-zhuo WANG Xiao-ping LUO +6 位作者 Gang-xiao REN Hong-xia WANG Li-fei WANG Wei-li CHENG Hang LI Xiao-peng LU Kwang-seon SHIN 《Transactions of Nonferrous Metals Society of China》 2025年第6期1772-1786,共15页
To investigate the effect of microstructure evolution on corrosion behavior and strengthening mechanism of Mg-1Zn-1Ca(wt.%)alloys,as-cast Mg-1Zn-1Ca alloys were performed by equal channel angular pressing(ECAP)with 1 ... To investigate the effect of microstructure evolution on corrosion behavior and strengthening mechanism of Mg-1Zn-1Ca(wt.%)alloys,as-cast Mg-1Zn-1Ca alloys were performed by equal channel angular pressing(ECAP)with 1 and 4 passes.The corrosion behavior and mechanical properties of alloys were investigated by optical microscopy(OM),scanning electron microscopy(SEM),electron backscatter diffraction(EBSD),electrochemical tests,immersion tests and tensile tests.The results showed that mechanical properties improved after ECAP 1 pass;however,the corrosion resistance deteriorated due to high-density dislocations and fragmented secondary phases by ECAP.In contrast,synchronous improvement in the mechanical properties and corrosion resistance was achieved though grain refinement after ECAP 4 passes;fine grains led to a significant improvement in the yield strength,ultimate tensile strength,elongation,and corrosion rate of 103 MPa,223 MPa,30.5%,and 1.5843 mm/a,respectively.The enhanced corrosion resistance was attributed to the formation of dense corrosion product films by finer grains and the barrier effect by high-density grain boundaries.These results indicated that Mg-1Zn-1Ca alloy has a promising potential for application in biomedical materials. 展开更多
关键词 Mg−1Zn−1Ca alloy equal channel angular pressing grain refinement corrosion behavior mechanical properties
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PPP-ss-O@Rg3胶束冷冻干燥保护剂的选择
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作者 贾莉 张卫敏 马兰 《广州化工》 2025年第10期108-110,114,共4页
通过考察冷冻干燥保护剂种类及比例,筛选出了PPP-ss-O@Rg3胶束的冷冻干燥保护剂。实验测定胶束溶液冷冻干燥后的外观、再分散速度、粒径和包封率,考察了多种糖类单独及联合使用作对胶束冻干过程保护作用。结果表明,在外观和再分散速度方... 通过考察冷冻干燥保护剂种类及比例,筛选出了PPP-ss-O@Rg3胶束的冷冻干燥保护剂。实验测定胶束溶液冷冻干燥后的外观、再分散速度、粒径和包封率,考察了多种糖类单独及联合使用作对胶束冻干过程保护作用。结果表明,在外观和再分散速度方面,甘露醇在略显优势;在粒径和包封率方面,海藻糖效果较为理想。进一步研究确定甘露醇-海藻糖(3%+7%)二元体系可显著优化PPP-ss-O@Rg3冻干制剂的理化特性。本研究为开发稳定完整的PPP-ss-O@Rg3胶束递送系统提供了理论依据。 展开更多
关键词 冷冻干燥保护剂 甘露醇 海藻糖 PPP-ss-O@Rg3胶束
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Ultrahigh strength and improved electrical conductivity in an aging strengthened copper alloy processed by combination of equal channel angular pressing and thermomechanical treatment 被引量:2
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作者 WANG Xu LI Zhou +1 位作者 MENG Xiang-peng XIAO Zhu 《Journal of Central South University》 SCIE EI CAS CSCD 2024年第6期1823-1837,共15页
In this paper,equal channel angular pressing and thermomechanical treatment was employed to improve the strength and electrical conductivity of an aging strengthened Cu-Ti-Cr-Mg alloy,and the microstructure and proper... In this paper,equal channel angular pressing and thermomechanical treatment was employed to improve the strength and electrical conductivity of an aging strengthened Cu-Ti-Cr-Mg alloy,and the microstructure and properties of the alloy were investigated in detail.The results showed that the samples deformed by the combination of cryogenic equal channel angular pressing(ECAP)and rolling had good comprehensive properties after aging at 400℃.The tensile strength of the peak-aged and over-aged samples was 1120 MPa and 940 MPa,with their corresponding electrical conductivity of 14.7%IACS and 22.1%IACS,respectively.ECAP and cryogenic rolling introduced high density dislocations,leading to the inhibition of the softening effects and refinement of the grains.After a long time aging at 400℃,the alloy exhibited ultra-high strength with obvious increasing electrical conductivity.The high strength was attributed to the synergistic effect of work hardening,grain refinement strengthening and precipitation strengthening.The precipitation of a large amount of Ti atoms from the matrix led to the high electrical conductivity of the over-aged sample. 展开更多
关键词 Cu-Ti alloy equal channel angular pressing ROLLING aging treatment high strength
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Ultra-low-loss all-fiber orbital angular momentum mode-division multiplexer based on cascaded fused-biconical mode selective couplers 被引量:2
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作者 Huiyi Guo Yan-ge Liu +4 位作者 *Liang Chen Wenzhe Chang Zekun Shi Letian Gu and Zhi Wang 《Advanced Photonics Nexus》 2024年第1期43-52,共10页
Mode-division multiplexers(MDMUXs)play a pivotal role in enabling the manipulation of an arbitrary optical state within few-mode fibers,offering extensive utility in the fields of mode-division multiplexing and struct... Mode-division multiplexers(MDMUXs)play a pivotal role in enabling the manipulation of an arbitrary optical state within few-mode fibers,offering extensive utility in the fields of mode-division multiplexing and structured optical field engineering.The exploration of MDMUXs employing cascaded resonant couplers has garnered significant attention owing to their scalability,exceptional integration capabilities,and the anticipated low insertion loss.In this work,we present the successful realization of high-quality orbital angular momentum MDMUX corresponding to topological charges 0,±1,and±2,achieved through the utilization of cascaded fused-biconical tapered couplers.Notably,the measured insertion losses at 1550 nm exhibit remarkable minimal values:0.31,0.10,and 0.64 dB,respectively.Furthermore,the 80%efficiency bandwidths exceed 106,174,and 174 nm for these respective modes.The MDMUX is composed of precisionmanufactured high-quality mode selective couplers(MSCs).Utilizing a proposed supermode propagation method based on mode composition analysis,we precisely describe the operational characteristics of MSCs.Building upon this comprehensive understanding,we embark on a pioneering analysis elucidating the influence of MSC cascading order on the performance of MDMUXs.Our theoretical investigation substantiates that when constructing MDMUXs,MSCs should adhere to a specific cascading sequence. 展开更多
关键词 space-division multiplexing all-fiber devices orbital angular momentum
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假结核耶尔森氏菌T6SS效应蛋白TseN功能鉴定
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作者 任兆锐 刘永德 +7 位作者 顾亚洲 杨彦涛 汪宇迪 宋莉 李长富 朱玲芳 王瑶 沈锡辉 《微生物学通报》 北大核心 2025年第4期1491-1506,共16页
【背景】细菌六型分泌系统(type VI secretion system,T6SS)广泛分布于革兰氏阴性细菌,在细菌定殖、环境适应、细菌-宿主互作等过程中发挥重要作用;T6SS对致病菌假结核耶尔森氏菌(Yersinia pseudotuberculosis)的致病性有重要作用。【... 【背景】细菌六型分泌系统(type VI secretion system,T6SS)广泛分布于革兰氏阴性细菌,在细菌定殖、环境适应、细菌-宿主互作等过程中发挥重要作用;T6SS对致病菌假结核耶尔森氏菌(Yersinia pseudotuberculosis)的致病性有重要作用。【目的】探究假结核耶尔森氏菌T6SS基因簇编码的潜在效应蛋白T6SS分泌型效应核酸酶(T6SS secreted effector nuclease,TseN)的生理生化特性及其生物学功能。【方法】通过生物信息学分析锁定潜在的T6SS效应蛋白TseN及其免疫蛋白T6SS分泌型免疫核酸酶(T6SS secreted immune nuclease,TsiN);构建相关重组质粒转化大肠杆菌(Escherichia coli),利用生长曲线测定等方法探究TseN的细菌毒性及TsiN免疫活性;通过细菌双杂交试验验证TseN与TsiN的相互作用;纯化蛋白,通过体外DNA酶活实验明确TseN的DNA酶活特性;利用同源重组等技术构建假结核耶尔森氏菌tseN基因缺失菌株(ΔtseN),并构建回补菌株[ΔtseN(tseN)],确证TseN在细菌竞争、抵抗胁迫等方面的功能。【结果】表达TseN的大肠杆菌生长受到显著抑制,共表达TsiN的菌株生长恢复,并且TsiN通过与TseN直接相互作用来中和其毒性。TseN可以结合DNA,具有Mn2+依赖的DNA水解酶活性。相较于野生型菌株,ΔtseN介导细菌种内或种间竞争的能力显著减弱,并且ΔtseN的生物膜形成受到显著影响,面对高盐、低pH等胁迫条件时存活率显著降低,以上情况在回补菌株中得到一定恢复。【结论】TseN是假结核耶尔森氏菌T6SS分泌的Mn2+依赖的DNA水解酶,具有较强的细菌毒性作用;TsiN是其免疫蛋白,保护假结核耶尔森氏菌免受TseN的毒性作用。TseN介导假结核耶尔森氏菌参与细菌竞争,有助于其占据生态位。同时,能促进假结核耶尔森氏菌的生物膜形成、高盐和低pH等胁迫耐受能力。 展开更多
关键词 假结核耶尔森氏菌 T6ss 效应蛋白 免疫蛋白 功能鉴定
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线粒体靶向肽SS-31对炎症诱导髓核细胞衰老和焦亡的作用及机制研究
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作者 彭鑫 王志强 +3 位作者 张桐 杨光 吴小涛 高延征 《中国脊柱脊髓杂志》 北大核心 2025年第4期399-407,共9页
目的:探讨线粒体靶向肽SS-31对炎症诱导的髓核(nucleus pulposus,NP)细胞衰老、焦亡的作用及机制。方法:体外提取培养6周龄SD雄性大鼠的NP细胞,分为对照组、脂多糖(lipopolysaccharide,LPS)组和LPS+SS-31组,其中对照组不做处理,LPS组以... 目的:探讨线粒体靶向肽SS-31对炎症诱导的髓核(nucleus pulposus,NP)细胞衰老、焦亡的作用及机制。方法:体外提取培养6周龄SD雄性大鼠的NP细胞,分为对照组、脂多糖(lipopolysaccharide,LPS)组和LPS+SS-31组,其中对照组不做处理,LPS组以浓度为100μg/mL的LPS干预NP细胞24h,LPS+SS-31组在LPS干预前30min利用SS-31进行预处理。采用衰老相关β半乳糖苷酶(senescence associatedβ-galactosidase,SA-β-Gal)染色和Western blot(WB)实验评估各组细胞衰老及焦亡水平;采用透射电镜和氧耗率检测分析各组NP细胞线粒体功能;应用WB和免疫荧光检测各组磷酸化核因子-κB-p65(phosphorylated nuclear factor-κB-p65,p-NF-κB-p65)、环磷酸鸟苷-腺苷酸合成酶(cyclic GMP-AMP synthase,cGAS)和干扰素刺激基因(interferon stimulating gene,STING)的蛋白表达;苏木精-伊红(hematoxylin-eosin,HE)染色、免疫组化(immunohistochemistry,IHC)染色和WB实验分析体内注射SS-31对椎间盘退变、细胞衰老和焦亡的影响。结果:与LPS组相比,LPS+SS-31组的NP细胞SA-β-Gal染色阳性率、衰老标志蛋白(p53、p21)及焦亡相关分子(NLRP3、Caspase-1 p20)的表达均明显降低(P<0.05)。通过透射电镜观察发现LPS组NP细胞线粒体肿胀呈圆形,基质透亮,线粒体嵴间腔扩张;而LPS+SS-31组的线粒体肿胀明显消退,线粒体形态趋于正常。同时,与LPS组相比,LPS+SS-31组的NP细胞最大呼吸水平明显增强(P<0.05)。WB和免疫荧光检测结果显示,LPS干预明显上调了p-NF-κB-p65、cGAS和STING的表达(P<0.05),而SS-31的预处理显著抑制了LPS诱导的p-NF-κB-p65、cGAS和STING的表达(P<0.05)。体内实验证实局部注射SS-31可降低NP细胞衰老、焦亡,减轻纤维环穿刺诱导的椎间盘退变。结论:SS-31减轻了炎症诱导的NP细胞衰老和焦亡,这与其对NF-κB信号通路、cGAS/STING信号通路的抑制有关。 展开更多
关键词 ss-31 髓核细胞 炎症 衰老 焦亡
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Bessel–Gaussian beam-based orbital angular momentum holography
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作者 季佳滢 郑志刚 +3 位作者 朱家龙 王乐 王新光 赵生妹 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第1期407-413,共7页
Orbital angular momentum(OAM), as a new degree of freedom, has recently been applied in holography technology.Due to the infinite helical mode index of OAM mode, a large number of holographic images can be reconstruct... Orbital angular momentum(OAM), as a new degree of freedom, has recently been applied in holography technology.Due to the infinite helical mode index of OAM mode, a large number of holographic images can be reconstructed from an OAM-multiplexing hologram. However, the traditional design of an OAM hologram is constrained by the helical mode index of the selected OAM mode, for a larger helical mode index OAM mode has a bigger sampling distance, and the crosstalk is produced for different sampling distances for different OAM modes. In this paper, we present the design of the OAM hologram based on a Bessel–Gaussian beam, which is non-diffractive and has a self-healing property during its propagation. The Fourier transform of the Bessel–Gaussian beam is the perfect vortex mode that has the fixed ring radius for different OAM modes. The results of simulation and experiment have demonstrated the feasibility of the generation of the OAM hologram with the Bessel–Gaussian beam. The quality of the reconstructed holographic image is increased, and the security is enhanced. Additionally, the anti-interference property is improved owing to its self-healing property of the Bessel-OAM holography. 展开更多
关键词 orbital angular momentum HOLOGRAPHY Bessel–Gaussian beam OAM-multiplexing hologram
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Solid state recycling of Mg-Gd-Y-Zn-Zr alloy chips by isothermal sintering and equal channel angular pressing
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作者 Yanbo Pei Hongjun Ma +1 位作者 Meng Yuan Bugang Teng 《Journal of Magnesium and Alloys》 SCIE EI CAS CSCD 2024年第7期2725-2740,共16页
The Mg-7Gd-4Y-2Zn-0.5Zr alloy chips were successfully recycled through isothermal sintering and equal channel angular pressing(ECAP).The mechanical properties and microstructure evolution of samples during the recycli... The Mg-7Gd-4Y-2Zn-0.5Zr alloy chips were successfully recycled through isothermal sintering and equal channel angular pressing(ECAP).The mechanical properties and microstructure evolution of samples during the recycling process were studied in detail.The eutectic phases in the as-cast alloy transform into long period-stacking ordered(LPSO)phases after homogenization,which can improve the plasticity of the material.After isothermal sintering,the density of the sample is lower than that of the homogenized sample,and oxide films are formed adjacent to the bonding interface of the metal chips.Hence,the plasticity of the sintered sample is poor.Dense samples are fabricated after ECAP.Although the grains are not refined compared to the sintered sample,the microstructure becomes more uniform due to recrystallization.Fiber interdendritic LPSO phase and kinked 14H-LPSO phase are formed in the alloy due to the shear deformation during the ECAP process,which improves the strength and plasticity of the sample significantly.Furthermore,the basal texture is weakened due to the Bc route of the ECAP process,which can increase the Schmid factor of the basal slip system and improve the elongation of the sample.After 2 ECAP passes,the fully densified recycled billet shows superior mechanical properties with an ultimate tensile strength of 307.1 MPa and elongation of 11.1%. 展开更多
关键词 Mg-Gd-Y-Zn-Zr alloy Solid state recycling Microstructure evolution LPSO phase Equal channel angular pressing
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基于CRISPR/Cas9技术的SS18-SSX1阳性细胞模型的构建与验证
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作者 田娇 张乾儒 +4 位作者 王宁 史奇 李亚 张琨岚 李锋 《石河子大学学报(自然科学版)》 北大核心 2025年第1期8-15,共8页
目的 利用CRISPR/Cas9技术构建并验证SS18-SSX1^(+)-HEK293T细胞模型。方法 靶向SS18和SSX1分别设计sgRNA,构建Lentivirus-CRISPR v2-SS18-gRNA-SSX1-gRNA-EGFP一体化慢病毒载体,通过慢病毒转染和荧光流式分选技术筛选转入载体的HEK293... 目的 利用CRISPR/Cas9技术构建并验证SS18-SSX1^(+)-HEK293T细胞模型。方法 靶向SS18和SSX1分别设计sgRNA,构建Lentivirus-CRISPR v2-SS18-gRNA-SSX1-gRNA-EGFP一体化慢病毒载体,通过慢病毒转染和荧光流式分选技术筛选转入载体的HEK293T多克隆细胞,在多克隆细胞的基础上挑选SS18-SSX1阳性的单克隆细胞株,通过RT-PCR、Sanger测序和Western Blot验证SS18-SSX1^(+)-HEK293T单克隆细胞系构建成功。结果 成功构建了Lentivirus-CRISPR v2-SS18-gRNA-SSX1-gRNA-EGFP慢病毒载体;通过RT-PCR、Sanger测序和WB实验在178个HEK293T单克隆细胞中成功筛选并验证出1株SS18-SSX1阳性的单克隆细胞系。结论 利用CRISPR/Cas9技术成功构建SS18-SSX1融合基因阳性的HEK293T细胞系。 展开更多
关键词 CRISPR/Cas9 滑膜肉瘤 ss18-ssX1 HEK293T细胞
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尿素变性PAGE中ssDNA回收方法的研究与优化
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作者 郑楚君 黄海轩 +3 位作者 王雨萱 叶煜玮 徐斐 袁敏 《工业微生物》 2025年第1期110-115,124,共7页
从变性PAGE中获得高纯度、高回收率的ss DNA,是功能核酸研究及适配体筛选的关键环节之一。文章采用直取法、改良煮沸法、试剂盒回收和正丁醇回收4种方法,在尿素变性PAGE中对ss DNA进行了提取回收研究,并发现改良煮沸法的ss DNA回收效率... 从变性PAGE中获得高纯度、高回收率的ss DNA,是功能核酸研究及适配体筛选的关键环节之一。文章采用直取法、改良煮沸法、试剂盒回收和正丁醇回收4种方法,在尿素变性PAGE中对ss DNA进行了提取回收研究,并发现改良煮沸法的ss DNA回收效率显著优于其他3种回收方法。对实验条件作进一步优化,使ss DNA回收效率达到了70.33±0.35%,并获得了较高的回收纯度(A260/A280=1.85)。可见,该种方法操作简便、回收速度快,且不易受有机试剂的干扰,同时成本低廉,单次回收成本不足1元,在功能核酸的筛选及回收利用方面具有良好的实际应用前景。 展开更多
关键词 尿素变性PAGE ss DNA回收 改良煮沸法
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含硫气井P110SS油管酸化-返排-生产全服役周期腐蚀特性的研究
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作者 罗伟 郭丽雪 +3 位作者 艾志鹏 侯铎 吴旭 张智 《材料保护》 2025年第10期157-167,共11页
为了明确四川盆地某含硫气井中P110SS油管在全服役周期连续多环境中的腐蚀损伤行为,利用高温高压釜模拟测试P110SS油管连续经历酸化压裂-残酸返排-生产工况的腐蚀特性。采用SEM、EDS、XRD和3D光学显微镜观察等方法分析了P110SS油管的腐... 为了明确四川盆地某含硫气井中P110SS油管在全服役周期连续多环境中的腐蚀损伤行为,利用高温高压釜模拟测试P110SS油管连续经历酸化压裂-残酸返排-生产工况的腐蚀特性。采用SEM、EDS、XRD和3D光学显微镜观察等方法分析了P110SS油管的腐蚀损伤特性,并对局部腐蚀坑尺寸进行了测量。结果表明:酸压工况下气相环境中的腐蚀速率为12.57 g/(m^(2)·h),远大于液相环境中的腐蚀速率2.06 g/(m^(2)·h),因此需要重点关注酸化压裂施工闷井阶段,在酸液面以上位置管柱的腐蚀损伤风险;连续经历多个工况下,P110SS油管在气相和液相腐蚀介质环境中的腐蚀速率为v_(鲜酸)>v_(鲜酸+残酸)>v_(鲜酸+残酸+地层水);鲜酸在140℃高温环境下缓蚀剂的效果良好,但在残酸返排工况中由于失去缓蚀剂的保护作用,管材出现严重点蚀;随着连续腐蚀工况的叠加,P110SS在气相和液相环境中的局部腐蚀都进一步加重。 展开更多
关键词 含硫气井 P110ss油管 全服役周期 腐蚀损伤
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SS-LMS自适应均衡算法的CTLE设计
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作者 唐明华 尤浩龙 +2 位作者 李刚 赵珍阳 陈建军 《国防科技大学学报》 北大核心 2025年第1期190-197,共8页
随着先进工艺和技术的不断进步,要想保证数据在高速传输中的正确性,均衡器需要有更高的补偿和更低的功耗,才能实现高效通信。基于12 nm互补金属氧化物半导体工艺,设计了一种高增益、低功耗的自适应连续时间线性均衡器(continuous time l... 随着先进工艺和技术的不断进步,要想保证数据在高速传输中的正确性,均衡器需要有更高的补偿和更低的功耗,才能实现高效通信。基于12 nm互补金属氧化物半导体工艺,设计了一种高增益、低功耗的自适应连续时间线性均衡器(continuous time linear equalizer,CTLE),该均衡器采用2级级联结构来补偿信道衰减,并提高接收信号的质量。此外,自适应模块通过采用符号-符号最小均方误差(sign-sign least mean square,SS-LMS)算法,使抽头系数加快了收敛速度。仿真结果表明,当传输速率为16 Gbit/s时,均衡器可以补偿-15.53 dB的半波特率通道衰减,均衡器系数在16×10^(4)个单元间隔数据内收敛,并且收敛之后接收误码率低于10^(-12)。 展开更多
关键词 连续时间线性均衡器 自适应 符号-符号最小均方误差算法
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“数字资本”何以影响学生的创造性——基于SSES2023的数据分析
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作者 张红 唐汉卫 +1 位作者 张珈瑜泽 李媛媛 《中国电化教育》 北大核心 2025年第5期53-60,94,共9页
创造性是大国竞争的关键。数字时代,数字资本对创造性的影响不容忽视,但其影响机理有待进一步揭示。该研究以参与SSES 2023调查的7648名中国学生为调查对象,采用OLS回归、Shapley值分解和分位数回归模型探究数字资源与数字能力所组成的... 创造性是大国竞争的关键。数字时代,数字资本对创造性的影响不容忽视,但其影响机理有待进一步揭示。该研究以参与SSES 2023调查的7648名中国学生为调查对象,采用OLS回归、Shapley值分解和分位数回归模型探究数字资源与数字能力所组成的数字资本对学生创造性的影响。研究发现:台式电脑、手提电脑/笔记本电脑、平板电脑和电子阅读器等数字资源均会对学生的创造性发展产生显著的正向影响,其中电子阅读器的影响效应最大;数字能力比数字资源更能促进学生创造性的提升,对学生创造性的贡献率可达28%;平板电脑和电子阅读器的持有量对学生创造性的提升呈现出边际效应递增作用,而手机持有量则对学生创造性的提升呈现出边际效应递减作用;数字能力的提升对高水平创造性的学生影响效益更大。研究建议:弥合数字资本鸿沟,推动学生创造性公平发展;优化数字资源配置,为学生创造性发展提供针对性支持;强化数字能力建设,促进数字资源向创造性高效转化;规避手机过度使用,多举措科学引导学生创造性发展。 展开更多
关键词 数字资本 创造性 ssES2023 分位数回归
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国际社会与情感能力测评的新进展与新趋向——基于OECD两轮SSES的经验与展望
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作者 唐一鹏 《中国考试》 北大核心 2025年第5期73-83,共11页
OECD社会与情感能力调查(SSES)已成功开展两轮测评,在国际社会引起较大反响。从两轮测评的实施比较来看,SSES在主题上从单向度的学习转向了多向度的幸福生活,参与国家数量不断增多,测评方法回归到学生自我汇报,测评题项更加丰富。对SSE... OECD社会与情感能力调查(SSES)已成功开展两轮测评,在国际社会引起较大反响。从两轮测评的实施比较来看,SSES在主题上从单向度的学习转向了多向度的幸福生活,参与国家数量不断增多,测评方法回归到学生自我汇报,测评题项更加丰富。对SSES的趋势数据进行国际比较分析发现,我国参与测评的小学生普遍表现良好,尤其在任务能力和协作能力两个维度上均名列国际前茅;我国中学生的表现大多处于中等水平,虽然在协作能力维度上表现较为突出,但在活力与好奇心上的表现存在明显不足。未来SSES将从概念框架、智能技术运用和教育实践三个方面寻求新的突破。我国的社会与情感能力测评研究尚处于起步阶段,SSES的经验具有重要借鉴价值。 展开更多
关键词 OECD ssES 社会与情感能力 国际测评 教育评价
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Achieving high-security and massive-capacity optical communications based on orbital angular momentum configured chaotic laser
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作者 Yanwei Cui Jianguo Zhang +2 位作者 Zhongquan Nie Anbang Wang Yuncai Wang 《Advanced Photonics Nexus》 2024年第4期92-100,共9页
Secure and high-speed optical communications are of primary focus in information transmission.Although it is widely accepted that chaotic secure communication can provide superior physical layer security,it is challen... Secure and high-speed optical communications are of primary focus in information transmission.Although it is widely accepted that chaotic secure communication can provide superior physical layer security,it is challenging to meet the demand for high-speed increasing communication rate.We theoretically propose and experimentally demonstrate a conceptual paradigm for orbital angular momentum(OAM)configured chaotic laser(OAM-CCL)that allows access to high-security and massivecapacity optical communications.Combining 11 OAM modes and an all-optical feedback chaotic laser,we are able to theoretically empower a well-defined optical communication system with a total transmission capacity of 100 Gb∕s and a bit error rate below the forward error correction threshold 3.8×10^(-3).Furthermore,the OAM-CCL-based communication system is robust to 3D misalignment by resorting to appropriate mode spacing and beam waist.Finally,the conceptual paradigm of the OAM-CCL-based communication system is verified.In contrast to existing systems(traditional free-space optical communication or chaotic optical communication),the OAM-CCL-based communication system has threein-one characteristics of high security,massive capacity,and robustness.The findings demonstrate that this will promote the applicable settings of chaotic laser and provide an alternative promising route to guide high-security and massive-capacity optical communications. 展开更多
关键词 chaotic secure communication orbital angular momentum configured chaotic laser high security massive capacity MISALIGNMENT
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