Objective To explore the biological basis of the damp-heat stasis-toxin syndrome in colorectal cancer(CRC)patients.Methods Totally 100 patients with CRC and 50 healthy individuals were screened.A total of 41 patients ...Objective To explore the biological basis of the damp-heat stasis-toxin syndrome in colorectal cancer(CRC)patients.Methods Totally 100 patients with CRC and 50 healthy individuals were screened.A total of 41 patients with CRC with damp heat stasis syndrome(SRYD),41 patients with CRC without damp heat stasis syndrome(FSRYD)and 40 healthy individuals(Normal)were enrolled.16S rRNA sequencing technology was used to analyze the differences in the composition and diversity of microbial groups.Results There was no significant difference in richness and diversity among SRYD,FSRYD and Normal groups.Compared with other groups,different expressions ofFirmicutes,Actinobacteriota,Elusimicrobiota,RCP2-54 at phylum level,Ralstonia,Sphingomonas,Dialister,Corynebacterium,Rothia,Odoribacter,Streptococcus and so on at genus level were detected in SRYDD groups.A possible noninvasive biomarker of damp-heat stasis andtoxin syndrome in colorectal cancer may be Streptococcus.LEfSe analysis indicated a ggreaterabundanceof Firmicutes,Actinobacteriota,Streptococcaceae,Streptococcus,Actinobacteria,Granulicatella,Rothia,Corynebacteriales,Corynebacteriaceae,Corynebacterium in SRYD groups.PICRUSt functional predictive analysis indicated the primary enrichment pathway of the SRYD group's differential expression are phosphotransferase system,valine,leucine and isoleucine degradation,central carbon metabolism in cancer,carbohydrate transport and metabolism.Conclusion It is reasonable to analyze CRC Chinese medicine syndrome differentiation from the perspective of tongue coating flora.Tongue coating flora can be used to diagnose damp-heat stasis-toxin syndrome in CRC patients.Streptococcus could be a noninvasive biomarker for damp-heat stasis-toxin syndrome in CRC.展开更多
文摘Objective To explore the biological basis of the damp-heat stasis-toxin syndrome in colorectal cancer(CRC)patients.Methods Totally 100 patients with CRC and 50 healthy individuals were screened.A total of 41 patients with CRC with damp heat stasis syndrome(SRYD),41 patients with CRC without damp heat stasis syndrome(FSRYD)and 40 healthy individuals(Normal)were enrolled.16S rRNA sequencing technology was used to analyze the differences in the composition and diversity of microbial groups.Results There was no significant difference in richness and diversity among SRYD,FSRYD and Normal groups.Compared with other groups,different expressions ofFirmicutes,Actinobacteriota,Elusimicrobiota,RCP2-54 at phylum level,Ralstonia,Sphingomonas,Dialister,Corynebacterium,Rothia,Odoribacter,Streptococcus and so on at genus level were detected in SRYDD groups.A possible noninvasive biomarker of damp-heat stasis andtoxin syndrome in colorectal cancer may be Streptococcus.LEfSe analysis indicated a ggreaterabundanceof Firmicutes,Actinobacteriota,Streptococcaceae,Streptococcus,Actinobacteria,Granulicatella,Rothia,Corynebacteriales,Corynebacteriaceae,Corynebacterium in SRYD groups.PICRUSt functional predictive analysis indicated the primary enrichment pathway of the SRYD group's differential expression are phosphotransferase system,valine,leucine and isoleucine degradation,central carbon metabolism in cancer,carbohydrate transport and metabolism.Conclusion It is reasonable to analyze CRC Chinese medicine syndrome differentiation from the perspective of tongue coating flora.Tongue coating flora can be used to diagnose damp-heat stasis-toxin syndrome in CRC patients.Streptococcus could be a noninvasive biomarker for damp-heat stasis-toxin syndrome in CRC.
