目的:建立可用于甘草HMGR,SQS1,β-AS合酶基因CNVs测定的稳定可靠的检测体系。方法:利用Real timePCR方法对甘草HMGR,SQS1,β-AS合酶基因CNVs进行测定。结果:在HMGR,SQS1,β-AS合酶基因以及内标基因lectin的定量检测实验中,其定量反应...目的:建立可用于甘草HMGR,SQS1,β-AS合酶基因CNVs测定的稳定可靠的检测体系。方法:利用Real timePCR方法对甘草HMGR,SQS1,β-AS合酶基因CNVs进行测定。结果:在HMGR,SQS1,β-AS合酶基因以及内标基因lectin的定量检测实验中,其定量反应的Ct值分别在25.82～25.88,29.01～29.08,15.52～15.56,19.06～19.08变化,SD分别是0.033,0.032,0.024,0.011,其CV分别是0.12%,0.22%,0.16%,0.06%。结论:所建立的Real time PCR体系重复性好,检测系统工作稳定可靠,可用于甘草HMGR,SQS1,β-AS基因的CNVs筛选。展开更多
Objective:To study the influence of over-expression of three functional genes involved in GC biosynthetic pathway,GuHMGR,GuSQS1,and GuBAS on GC production.Methods:Three plant expression vectors were constructed and tr...Objective:To study the influence of over-expression of three functional genes involved in GC biosynthetic pathway,GuHMGR,GuSQS1,and GuBAS on GC production.Methods:Three plant expression vectors were constructed and transformed into Agrobacterium tumefaciens EHA105,which were used to infect Glycyrrhiza uralensis hypocotyls explants.After induction,selection,differentiation,culture,and transplantation,12,15,and 5 regenerated plants over-expressing GuHMGR,GuSQS1,and GuBAS,were obtained,respectively.Results:RT-PCR analysis showed these transgenic regenerated G.uralensis plants had 2-6 copies of GuHMGR,GuSQS1,or GuBAS.HPLC analysis showed the contents of 18α-and 18β-GC in all transgenic regenerated samples were both higher than that in the blank control.With the increase of copy numbers of GuHMGR,GuSQS1,and GuBAS,the contents of 18α-and 18β-GC were both increased in most samples.The highest 18α-and 18β-GC contents in transgenic regenerated plants were about 3.05 times and 2.80 times higher than that in the blank control,respectively.Conclusion:Over-expression of the GuHMGR,GuSQS1,and GuBAS genes enhance the accumulation of 18α-and 18β-GC in the roots and rhizomes of G.uralensis.We hope this work can lay a foundation for the molecular breeding research of G.uralensis and improving the quality of the roots and rhizomes of G.uralensis cultivars.展开更多
Glycyrrhiza uralensis is frequently used in traditional Chinese medicine. This plant contains a large amount of effective constituents, including triterpenoids and flavonoids. Among them, glycyrrhizin is believed to b...Glycyrrhiza uralensis is frequently used in traditional Chinese medicine. This plant contains a large amount of effective constituents, including triterpenoids and flavonoids. Among them, glycyrrhizin is believed to be the marker compound to evaluate the quality of G. uralensis based on Chinese Pharmacopoeia. Many studies showed that glycyrrhizin possesses various pharmacological activities, such as antibacterial, antiviral, antitumor, anti-inflammatory, and immune-stimulating activities. In this paper, we summarized the cloning, characterization, expression, and polymorphism analysis of several functional genes involved in glycyrrhizin biosynthesis in G. uralensis.展开更多
The Sq(solar quiet)geomagnetic field is generated by the electric currents in the E-region of the ionosphere,driven by the atmospheric tides.It is a critical part of high-precision geomagnetic field modeling.Based on ...The Sq(solar quiet)geomagnetic field is generated by the electric currents in the E-region of the ionosphere,driven by the atmospheric tides.It is a critical part of high-precision geomagnetic field modeling.Based on the classic thermal tide theory and atmospheric electrodynamics,this research,for the first time,developed an Sq geomagnetic field model that is directly built on the physical mechanism of the ionospheric dynamo,which is responsible for daily variations of the geomagnetic field.The performance in Sq geomagnetic field modeling was investigated using the Macao Science Satellite-1(MSS-1)data.Our model can enhance the physics-based framework of comprehensive geomagnetic field modeling for the MSS-1 and ensuing missions.展开更多
文摘目的:建立可用于甘草HMGR,SQS1,β-AS合酶基因CNVs测定的稳定可靠的检测体系。方法:利用Real timePCR方法对甘草HMGR,SQS1,β-AS合酶基因CNVs进行测定。结果:在HMGR,SQS1,β-AS合酶基因以及内标基因lectin的定量检测实验中,其定量反应的Ct值分别在25.82～25.88,29.01～29.08,15.52～15.56,19.06～19.08变化,SD分别是0.033,0.032,0.024,0.011,其CV分别是0.12%,0.22%,0.16%,0.06%。结论:所建立的Real time PCR体系重复性好,检测系统工作稳定可靠,可用于甘草HMGR,SQS1,β-AS基因的CNVs筛选。
文摘Objective:To study the influence of over-expression of three functional genes involved in GC biosynthetic pathway,GuHMGR,GuSQS1,and GuBAS on GC production.Methods:Three plant expression vectors were constructed and transformed into Agrobacterium tumefaciens EHA105,which were used to infect Glycyrrhiza uralensis hypocotyls explants.After induction,selection,differentiation,culture,and transplantation,12,15,and 5 regenerated plants over-expressing GuHMGR,GuSQS1,and GuBAS,were obtained,respectively.Results:RT-PCR analysis showed these transgenic regenerated G.uralensis plants had 2-6 copies of GuHMGR,GuSQS1,or GuBAS.HPLC analysis showed the contents of 18α-and 18β-GC in all transgenic regenerated samples were both higher than that in the blank control.With the increase of copy numbers of GuHMGR,GuSQS1,and GuBAS,the contents of 18α-and 18β-GC were both increased in most samples.The highest 18α-and 18β-GC contents in transgenic regenerated plants were about 3.05 times and 2.80 times higher than that in the blank control,respectively.Conclusion:Over-expression of the GuHMGR,GuSQS1,and GuBAS genes enhance the accumulation of 18α-and 18β-GC in the roots and rhizomes of G.uralensis.We hope this work can lay a foundation for the molecular breeding research of G.uralensis and improving the quality of the roots and rhizomes of G.uralensis cultivars.
基金Beijing Project for Young Talents(YETP0819)National Science Foundation of China(81503181)
文摘Glycyrrhiza uralensis is frequently used in traditional Chinese medicine. This plant contains a large amount of effective constituents, including triterpenoids and flavonoids. Among them, glycyrrhizin is believed to be the marker compound to evaluate the quality of G. uralensis based on Chinese Pharmacopoeia. Many studies showed that glycyrrhizin possesses various pharmacological activities, such as antibacterial, antiviral, antitumor, anti-inflammatory, and immune-stimulating activities. In this paper, we summarized the cloning, characterization, expression, and polymorphism analysis of several functional genes involved in glycyrrhizin biosynthesis in G. uralensis.
基金supported by the National Natural Science Foundation of China(Grant Nos.12250013,12403070,12425306,42250101,12273092)the Macao Foundation,and Shanghai Post-doctoral Excellence Program(Grant No.2023000137)。
文摘The Sq(solar quiet)geomagnetic field is generated by the electric currents in the E-region of the ionosphere,driven by the atmospheric tides.It is a critical part of high-precision geomagnetic field modeling.Based on the classic thermal tide theory and atmospheric electrodynamics,this research,for the first time,developed an Sq geomagnetic field model that is directly built on the physical mechanism of the ionospheric dynamo,which is responsible for daily variations of the geomagnetic field.The performance in Sq geomagnetic field modeling was investigated using the Macao Science Satellite-1(MSS-1)data.Our model can enhance the physics-based framework of comprehensive geomagnetic field modeling for the MSS-1 and ensuing missions.
