Glucosinolates are important phytochemicals in Brassicaceae.We investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli sprouts.The results showed that CHEW treatment ...Glucosinolates are important phytochemicals in Brassicaceae.We investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli sprouts.The results showed that CHEW treatment significantly decreased reactive oxygen species(ROS)and malondialdeh yde(MDA)contents in broccoli sprouts.On the the 8^(th)day,compared to tap water treatment,the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6%and calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g DW.Comparative transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK cascades.Besides,CHEW treatment not only promoted the biosynthesis of amino acids,but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors(MYBs,bHLHs,WRKYs,etc.).The results of this study provided new insights into the regulatory network of glucosinolates biosynthesis in broccoli sprouts under CHEW treatment.展开更多
A new electrolyzed water-CaCl_(2)-HCl electrolyzed water(CHEW)was obtained by combining slightly acidic electrolyzed water and calcium.the objective of this study was to explore the effect of preharvest CHEW treatment...A new electrolyzed water-CaCl_(2)-HCl electrolyzed water(CHEW)was obtained by combining slightly acidic electrolyzed water and calcium.the objective of this study was to explore the effect of preharvest CHEW treatment on the quality during storage of broccoli sprouts.Preharvest CHEW treatment not only reduced the electrolyte leakage,reactive oxygen species,weight loss and the number of microorganisms during storage of broccoli sprouts,but also slowed down the breakdown of cell structure and maintained color and textural properties of broccoli sprouts.besides,preharvest CHEW treatment reduced the degradation of active substances in broccoli sprouts during storage.the contents of important bioactive compounds in broccoli sprouts-glucoraphanin and sulforaphane,along with the enzymatic activity and genes involved in glucosinolates metabolism have also been further investigated.on day 12 of storage,compared to preharvest tap water treatment,the contents of glucoraphanin and sulforaphane in broccoli sprouts with preharvest CHEW treatment increased by 63%and 128%,respectively.Meanwhile,the generation of offensive odors in broccoli sprouts during storage was also inhibited under preharvest CHEW treatment.in general,preharvest CHEW treatment maintained the good quality during storage and prolong the shelf life of broccoli sprouts.展开更多
The growing recognition of a healthy lifestyle framework has promoted the development of novel functional foods or superfoods with abundant nutrients and health-promoting properties.Therefore,the influence of slightly...The growing recognition of a healthy lifestyle framework has promoted the development of novel functional foods or superfoods with abundant nutrients and health-promoting properties.Therefore,the influence of slightly acidic electrolyzed water(SAEW)as an elicitor on broccoli sprouts was investigated.The increase of electrolyte leakage percentage,thiobarbituric acid reactive substances levels and proline concentration indicated that the defense system of broccoli sprouts had responded to stress caused by SAEW.Furthermore,the antioxidant enzymes and secondary metabolites,phenolic compounds,were evaluated and revealed the enhancement of antioxidant enzymes(superoxide dismutase,peroxidases,catalase,and ascorbate peroxidase)activities and phenolic compounds content in broccoli sprouts with SAEW treatment.The major groups of phenolic composition in broccoli sprouts were phenolic acids and flavonoids.The Folin-Ciocalteu index and antioxidant activity assay also proved the enhancement of antioxidant capacity of broccoli sprouts with SAEW treatment.Results from this study indicated that SAEW treatment could increase the antioxidant ability of broccoli sprouts.展开更多
基金supported by the National Natural Science Foundation of China(31972091)。
文摘Glucosinolates are important phytochemicals in Brassicaceae.We investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli sprouts.The results showed that CHEW treatment significantly decreased reactive oxygen species(ROS)and malondialdeh yde(MDA)contents in broccoli sprouts.On the the 8^(th)day,compared to tap water treatment,the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6%and calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g DW.Comparative transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK cascades.Besides,CHEW treatment not only promoted the biosynthesis of amino acids,but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors(MYBs,bHLHs,WRKYs,etc.).The results of this study provided new insights into the regulatory network of glucosinolates biosynthesis in broccoli sprouts under CHEW treatment.
基金supported by the National Natural Science Foundation of China(31972091)Hebei Province Science and technology Support Program(226Z7102G).
文摘A new electrolyzed water-CaCl_(2)-HCl electrolyzed water(CHEW)was obtained by combining slightly acidic electrolyzed water and calcium.the objective of this study was to explore the effect of preharvest CHEW treatment on the quality during storage of broccoli sprouts.Preharvest CHEW treatment not only reduced the electrolyte leakage,reactive oxygen species,weight loss and the number of microorganisms during storage of broccoli sprouts,but also slowed down the breakdown of cell structure and maintained color and textural properties of broccoli sprouts.besides,preharvest CHEW treatment reduced the degradation of active substances in broccoli sprouts during storage.the contents of important bioactive compounds in broccoli sprouts-glucoraphanin and sulforaphane,along with the enzymatic activity and genes involved in glucosinolates metabolism have also been further investigated.on day 12 of storage,compared to preharvest tap water treatment,the contents of glucoraphanin and sulforaphane in broccoli sprouts with preharvest CHEW treatment increased by 63%and 128%,respectively.Meanwhile,the generation of offensive odors in broccoli sprouts during storage was also inhibited under preharvest CHEW treatment.in general,preharvest CHEW treatment maintained the good quality during storage and prolong the shelf life of broccoli sprouts.
基金supported by the National Key Research and Development Program(2017YFE0114500-4)the National Science Foundation of China(NSFC),“Regulations of production conditions and transformation mechanism of sulforaphane in broccoli sprouts with CaCl2-HCl electrolyzed water”(No.31972091).
文摘The growing recognition of a healthy lifestyle framework has promoted the development of novel functional foods or superfoods with abundant nutrients and health-promoting properties.Therefore,the influence of slightly acidic electrolyzed water(SAEW)as an elicitor on broccoli sprouts was investigated.The increase of electrolyte leakage percentage,thiobarbituric acid reactive substances levels and proline concentration indicated that the defense system of broccoli sprouts had responded to stress caused by SAEW.Furthermore,the antioxidant enzymes and secondary metabolites,phenolic compounds,were evaluated and revealed the enhancement of antioxidant enzymes(superoxide dismutase,peroxidases,catalase,and ascorbate peroxidase)activities and phenolic compounds content in broccoli sprouts with SAEW treatment.The major groups of phenolic composition in broccoli sprouts were phenolic acids and flavonoids.The Folin-Ciocalteu index and antioxidant activity assay also proved the enhancement of antioxidant capacity of broccoli sprouts with SAEW treatment.Results from this study indicated that SAEW treatment could increase the antioxidant ability of broccoli sprouts.
