Forced dissociation of selectin-ligand bonds is crucial to such biological processes as leukocyte recruitment, thrombosis formation, as well as tumor metastasis. While
This study represents the influence on exhaust performance by active muffler valve spring.The main experimental parameters were engine speeds and torsion coil spring constants in the active muffler.When the muffler va...This study represents the influence on exhaust performance by active muffler valve spring.The main experimental parameters were engine speeds and torsion coil spring constants in the active muffler.When the muffler valve spring has a low spring constant of k=0.75,the sound pressure level was low at the region of engine speed 2000 to 2500 r/min,and the velocity of exhaust gas was high at spring constant k=0.75.When the engine speed was under 1500 r/min,the mean concentration of CO was high in case without muffler valve.When the engine speed in the range of 1600 to 2600 r/min with spring constant k=0.75,the mean HC concentration showed the lowest value.Without muffler valve,the temperature in the muffler was higher than the case with muffler valve.This research represented that the performance of the active muffler valve spring constant k=0.75 was higher than that without muffler valve and valve spring constant k=0.70.Among the three cases of experimental condition,with a spring constant k=0.75,0.70 and without muffler valve,the exhaust performance was high in case active muffler valve spring constant k=0.70.展开更多
This article discussed how to improve constant force performance of the large capacity constant spring support hangers. Large deviations have been found between product testing results and theoretical calculations aft...This article discussed how to improve constant force performance of the large capacity constant spring support hangers. Large deviations have been found between product testing results and theoretical calculations after years of research and testing. The deviations mainly are caused by internal frictions inside the constant spring support hangers. By reducing or properly using internal frictions, consistent results between testing and theoretical calculation have been achieved. Based on product performance testing results recently, constant force performance of hangers has been improved greatly by adopting new methodology.展开更多
鲤春病毒血症病毒(spring viremia of carp virus,SVCV)是一种高致病性病原,对鲤科鱼类造成巨大危害,被世界动物卫生组织列为必须通报疫病。2002年,鲤春病毒血症(spring viremia of carp,SVC)在中国首次检出后,迅速在全国蔓延,给我国鲤...鲤春病毒血症病毒(spring viremia of carp virus,SVCV)是一种高致病性病原,对鲤科鱼类造成巨大危害,被世界动物卫生组织列为必须通报疫病。2002年,鲤春病毒血症(spring viremia of carp,SVC)在中国首次检出后,迅速在全国蔓延,给我国鲤鱼养殖业造成巨大威胁。因此,我国农业农村部将其列为二类动物疫病。迄今为止,早期、快速、准确的诊断仍然是控制其传播和流行的重要手段。本研究基于CRISPR/Cas13a系统结合重组酶介导等温核酸扩增技术(recombinase-aided amplification,RAA),通过对GenBank上登录的SVCV全基因序列比对,针对SVCV聚合酶L基因高度保守的区域设计了一组RAA扩增引物和相应crRNA;建立了一种可用于SVCV现场快速检测的RAA-CRISPR/Cas13a的方法,可以覆盖SVCV的4种不同基因型(Ia、Ib、Ic和Id)。按照世界动物卫生组织推荐的检测方法验证程序,经实验验证该检测方法有较好的重复性,最低检出限为115 copies/μL,与其他病原之间不发生交叉反应。通过对实验室分离保存的60份来自进出境水生动物疫病监测、国内重大水生动物疫病监测以及攻毒实验的样本进行检测,RAA-CRISPR/Cas13a检测结果与实时荧光定量PCR、套式RT-PCR和病毒分离培养结果完全一致,诊断灵敏度和诊断特异性均达到100%。采用本研究建立的方法在4个不同实验室开展比对,在相同条件下对20份样本进行检测,4个实验室的检测结果一致。结果表明,本研究建立的方法重现性良好,为首次采用CRISPR-Cas13a系统进行SVCV检测的研究,对SVCV快速诊断和防控具有重要现实意义。展开更多
基金Supported by National Natural Science Foundation of China grants 30730032 and 10702075National Key Basic Research Foundation of China grant 2006CB910303+1 种基金National High Technology Research and Development Program of China grant 2007AA02Z306Chinese Academy of Sciences grant KJCX2-YW-L08
文摘Forced dissociation of selectin-ligand bonds is crucial to such biological processes as leukocyte recruitment, thrombosis formation, as well as tumor metastasis. While
基金This work was supported by the Brain Korea 21 Projects,and the authors gratefully appreciate the support.
文摘This study represents the influence on exhaust performance by active muffler valve spring.The main experimental parameters were engine speeds and torsion coil spring constants in the active muffler.When the muffler valve spring has a low spring constant of k=0.75,the sound pressure level was low at the region of engine speed 2000 to 2500 r/min,and the velocity of exhaust gas was high at spring constant k=0.75.When the engine speed was under 1500 r/min,the mean concentration of CO was high in case without muffler valve.When the engine speed in the range of 1600 to 2600 r/min with spring constant k=0.75,the mean HC concentration showed the lowest value.Without muffler valve,the temperature in the muffler was higher than the case with muffler valve.This research represented that the performance of the active muffler valve spring constant k=0.75 was higher than that without muffler valve and valve spring constant k=0.70.Among the three cases of experimental condition,with a spring constant k=0.75,0.70 and without muffler valve,the exhaust performance was high in case active muffler valve spring constant k=0.70.
文摘This article discussed how to improve constant force performance of the large capacity constant spring support hangers. Large deviations have been found between product testing results and theoretical calculations after years of research and testing. The deviations mainly are caused by internal frictions inside the constant spring support hangers. By reducing or properly using internal frictions, consistent results between testing and theoretical calculation have been achieved. Based on product performance testing results recently, constant force performance of hangers has been improved greatly by adopting new methodology.
文摘鲤春病毒血症病毒(spring viremia of carp virus,SVCV)是一种高致病性病原,对鲤科鱼类造成巨大危害,被世界动物卫生组织列为必须通报疫病。2002年,鲤春病毒血症(spring viremia of carp,SVC)在中国首次检出后,迅速在全国蔓延,给我国鲤鱼养殖业造成巨大威胁。因此,我国农业农村部将其列为二类动物疫病。迄今为止,早期、快速、准确的诊断仍然是控制其传播和流行的重要手段。本研究基于CRISPR/Cas13a系统结合重组酶介导等温核酸扩增技术(recombinase-aided amplification,RAA),通过对GenBank上登录的SVCV全基因序列比对,针对SVCV聚合酶L基因高度保守的区域设计了一组RAA扩增引物和相应crRNA;建立了一种可用于SVCV现场快速检测的RAA-CRISPR/Cas13a的方法,可以覆盖SVCV的4种不同基因型(Ia、Ib、Ic和Id)。按照世界动物卫生组织推荐的检测方法验证程序,经实验验证该检测方法有较好的重复性,最低检出限为115 copies/μL,与其他病原之间不发生交叉反应。通过对实验室分离保存的60份来自进出境水生动物疫病监测、国内重大水生动物疫病监测以及攻毒实验的样本进行检测,RAA-CRISPR/Cas13a检测结果与实时荧光定量PCR、套式RT-PCR和病毒分离培养结果完全一致,诊断灵敏度和诊断特异性均达到100%。采用本研究建立的方法在4个不同实验室开展比对,在相同条件下对20份样本进行检测,4个实验室的检测结果一致。结果表明,本研究建立的方法重现性良好,为首次采用CRISPR-Cas13a系统进行SVCV检测的研究,对SVCV快速诊断和防控具有重要现实意义。
