Objective: to explore the healing-promoting effect of quercetin wound-healing sponge dressing on the back wound of SD rats. Methods: SD rats were randomly divided into quercetin dressing group, blank control group and...Objective: to explore the healing-promoting effect of quercetin wound-healing sponge dressing on the back wound of SD rats. Methods: SD rats were randomly divided into quercetin dressing group, blank control group and vaseline gauze group, with 10 rats in each group. Full-thickness skin excision was performed to establish a back wound model, and local dressing change was started on the day of model establishment;The wound healing rate was calculated on days 0, 3, 7 and 10 of dressing change, and the new tissue of wound healing site on day 7 of dressing change was collected for HE staining. Results: on the 10th day of dressing change, the healing rate of the back wound of SD rats in the quercetin dressing group was significantly higher than that of the blank control group and the vaseline gauze group (P < 0.05). The collagen staining intensity, inflammatory cell distribution, angiogenesis rate and fibroblast distribution score in the wound tissue of SD rats in quercetin dressing group were significantly higher than those in the blank control group and vaseline gauze group (p < 0.05). Conclusion: quercetin wound-healing sponge dressing can promote the healing of the wounds on the back of SD rats with good efficacy, which is worthy of further scientific verification for clinical marketing.展开更多
A porous,biocompatible,and modifiable chitosan (CS)/poly (vinyl alcohol) (PVA) hybrid membrane, which can be evaluated as wound dressing,has been prepared by lyophilization method and using the low toxicity solvents. ...A porous,biocompatible,and modifiable chitosan (CS)/poly (vinyl alcohol) (PVA) hybrid membrane, which can be evaluated as wound dressing,has been prepared by lyophilization method and using the low toxicity solvents. Experiments illustrated that the moisture regain and porosity rate of polymer blend membranes had the similar variation tendency and got the maximum value at the case of CS/PVA weight ratio of 6∶4. And the value of water vapor transmission rate( WVTR) is conductive to the healing of wound and desirable for the application as a wound dressing.Mechanical performance test of blend membrane showed that the presence of PVA could effectively improve the interaction between CS molecules. The scanning electron microscopy( SEM)photograph confirmed the porous surface on top-layer supported by a macroporous sponge-like sub-layer of this novel type of CS blend membrane. Besides, Fourier transform infrared spectroscopy( FTIR) revealed the facts that the two polymers were physically bended and certain chemical actions took place,which illustrated the good compatibility between them. Differential scanning calorimetry(DSC) results showed that decomposition reaction occurred in CS at about 210℃. Introducing of PVA to CS made the decomposition temperature of blend membrane decrease. The good properties,such as moisture regain,porosity rate,and WVTR,of this kind of CS/PVA blend membranes suggested a potential application as a wound dressing in the future.展开更多
文摘Objective: to explore the healing-promoting effect of quercetin wound-healing sponge dressing on the back wound of SD rats. Methods: SD rats were randomly divided into quercetin dressing group, blank control group and vaseline gauze group, with 10 rats in each group. Full-thickness skin excision was performed to establish a back wound model, and local dressing change was started on the day of model establishment;The wound healing rate was calculated on days 0, 3, 7 and 10 of dressing change, and the new tissue of wound healing site on day 7 of dressing change was collected for HE staining. Results: on the 10th day of dressing change, the healing rate of the back wound of SD rats in the quercetin dressing group was significantly higher than that of the blank control group and the vaseline gauze group (P < 0.05). The collagen staining intensity, inflammatory cell distribution, angiogenesis rate and fibroblast distribution score in the wound tissue of SD rats in quercetin dressing group were significantly higher than those in the blank control group and vaseline gauze group (p < 0.05). Conclusion: quercetin wound-healing sponge dressing can promote the healing of the wounds on the back of SD rats with good efficacy, which is worthy of further scientific verification for clinical marketing.
基金Natural Science Foundation of Shanghai,China(No.14ZR1401000)Shanghai University Students Innovation Project of China(No.sh201410255026)National Natural Science Foundation of China(No.51303021)
文摘A porous,biocompatible,and modifiable chitosan (CS)/poly (vinyl alcohol) (PVA) hybrid membrane, which can be evaluated as wound dressing,has been prepared by lyophilization method and using the low toxicity solvents. Experiments illustrated that the moisture regain and porosity rate of polymer blend membranes had the similar variation tendency and got the maximum value at the case of CS/PVA weight ratio of 6∶4. And the value of water vapor transmission rate( WVTR) is conductive to the healing of wound and desirable for the application as a wound dressing.Mechanical performance test of blend membrane showed that the presence of PVA could effectively improve the interaction between CS molecules. The scanning electron microscopy( SEM)photograph confirmed the porous surface on top-layer supported by a macroporous sponge-like sub-layer of this novel type of CS blend membrane. Besides, Fourier transform infrared spectroscopy( FTIR) revealed the facts that the two polymers were physically bended and certain chemical actions took place,which illustrated the good compatibility between them. Differential scanning calorimetry(DSC) results showed that decomposition reaction occurred in CS at about 210℃. Introducing of PVA to CS made the decomposition temperature of blend membrane decrease. The good properties,such as moisture regain,porosity rate,and WVTR,of this kind of CS/PVA blend membranes suggested a potential application as a wound dressing in the future.
