目的探究瑞马唑仑(RZ)调节Notch-1/发状分裂相关增强子(Hes)信号通路对烧伤脓毒症大鼠肠道损伤的影响。方法将60只SD大鼠随机分为正常组(Normal组)、模型组(Model组)、低剂量RZ组(RZ-L组,3mg/kg RZ)、高剂量RZ组(RZ-H组,6mg/kg RZ)和高...目的探究瑞马唑仑(RZ)调节Notch-1/发状分裂相关增强子(Hes)信号通路对烧伤脓毒症大鼠肠道损伤的影响。方法将60只SD大鼠随机分为正常组(Normal组)、模型组(Model组)、低剂量RZ组(RZ-L组,3mg/kg RZ)、高剂量RZ组(RZ-H组,6mg/kg RZ)和高剂量RZ+Notch-1激活剂Jagged1组(RZ-H+Jagged1组,6mg/kg RZ+0.67mg/kg Jagged1),每组12只。在大鼠背部建立20%总体表面积(TBSA)Ⅲ度烫伤(烧伤)模型,之后注射铜绿假单胞菌制备烧伤脓毒症模型。HE染色观察回肠组织病理变化,并进行肠黏膜损伤评分(Chiu’s评分)。ELISA法检测血清内毒素、D-乳酸和二胺氧化酶(DAO)及回肠组织白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)、髓过氧化物酶(MPO)的水平。RT-qPCR法检测回肠组织Notch-1,Hes mRNA表达。Western Blot检测回肠组织Notch-1/Hes信号通路相关蛋白表达。结果与Normal组相比,Model组大鼠肠黏膜损伤评分(5.30±0.48分vs 1.00±0.52分)、血清内毒素(135.69±16.24 pg/ml vs 62.48±3.23 pg/ml),D-乳酸(1403.58±68.97 pg/ml vs 752.06±25.29 pg/ml)和DAO水平(137.55±12.37 pg/ml vs 58.52±4.43 pg/ml)、回肠组织IL-6(186.45±16.37 ng/ml vs 21.32±3.66 ng/ml),TNF-α(163.37±11.40 ng/ml vs 15.52±1.03 ng/ml)和MPO水平(8.94±0.92 U/ml vs 2.35±0.33 U/ml)及Notch-1 mRNA(1.86±0.19 vs 1.00±0.10)和蛋白(0.79±0.08 vs 0.21±0.02),Hes-1 mRNA(2.13±0.21 vs 1.00±0.12)和蛋白(0.88±0.09 vs 0.32±0.03)表达水平均升高,差异具有统计学意义(t=14.294~47.368,均P<0.05)。与Model组相比,RZ-L组和RZ-H组相应指标变化与上述相反,差异具有统计学意义(tRZ-L=5.650~21.448,tRZ-H=4.435~42.403,均P<0.05)。Notch-1激活剂Jagged1减弱了RZ对烧伤脓毒症大鼠肠道损伤的治疗作用。结论RZ可能通过抑制Notch-1/Hes信号通路减轻烧伤脓毒症模型大鼠的肠道损伤。展开更多
Temporal lobe epilepsy is associated with astrogliosis. Notchl signaling can induce astrogliosis in glioma. However, it remains unknown whether Notchl signaling is involved in the pathogenesis of epilepsy. This study ...Temporal lobe epilepsy is associated with astrogliosis. Notchl signaling can induce astrogliosis in glioma. However, it remains unknown whether Notchl signaling is involved in the pathogenesis of epilepsy. This study investigated the presence of Notchl, hairy and enhancer of split-l, and glial fibrillary acidic protein in the temporal neocortex and hippocampus of lithium-pilocar- pine-treated rats. The presence of Notchl and hairy and enhancer of split-1 was also explored in brain tissues of patients with intractable temporal lobe epilepsy. Quantitative electroencephalo- gram analysis and behavioral observations were used as auxiliary measures. Results revealed that the presence of Notchl, hairy and enhancer of split-l, and glial fibriUary acidic protein were en- hanced in status epilepticus and vehicle-treated spontaneous recurrent seizures rats, but remain unchanged in the following groups： control, absence of either status epilepticus or spontaneous recurrent seizures, and zileuton-treated spontaneous recurrent seizures. Compared with patient control cases, the presences of Notch1 and hairy and enhancer of split- 1 were upregulated in the temporal neocortex of patients with intractable temporal lobe epilepsy. Therefore, these results suggest that Notchl signaling may play an important role in the onset of temporal lobe epilepsy via astrogliosis. Furthermore, zileuton may be a potential therapeutic strategy for temporal lobe epilepsy by blocking Notchl signaling.展开更多
目的观察骶1节段多裂肌劈开入路微创经锥间孔腰椎锥间融合术(MIS-TILF)治疗腰椎滑脱症的临床疗效。方法选择2017年2月-2018年2月期间在我院接受治疗的腰椎滑脱症患者98例,按照随机数字表法分为对照组和观察组,每组各49例。给予对照组患...目的观察骶1节段多裂肌劈开入路微创经锥间孔腰椎锥间融合术(MIS-TILF)治疗腰椎滑脱症的临床疗效。方法选择2017年2月-2018年2月期间在我院接受治疗的腰椎滑脱症患者98例,按照随机数字表法分为对照组和观察组,每组各49例。给予对照组患者常规正中骶棘肌剥离入路MIS-TILF治疗,给予观察组患者骶1节段多裂肌劈开入路MIS-TILF治疗。比较两组患者治疗前后腰椎功能、术后疼痛情况以及术中出血量、手术时间、术后引流量等手术相关指标。结果两组患者术前的ODI、JOA评分差异无统计学意义(P>0.05);术后,观察组患者ODI评分低于对照组,JOA评分高于对照组,差异具有统计学意义(P<0.05);术前,两组患者的疼痛VAS评分差异无统计学意义(P> 0.05);观察组患者术后3 d、14 d VAS评分均低于对照组,差异有统计学意义(P<0.05);观察组患者术中出血量、手术时间及术后引流量低于对照组,住院时间短于对照组,差异有统计学意义(P<0.05)。结论与传统正中骶棘肌剥离入路MIS-TILF治疗比较,骶1节段多裂肌劈开入路MIS-TILF在腰椎滑脱症的治疗中可有效促进患者腰椎功能恢复,同时具有术后疼痛小、术中出血少、术后恢复快等优势。展开更多
文摘目的探究瑞马唑仑(RZ)调节Notch-1/发状分裂相关增强子(Hes)信号通路对烧伤脓毒症大鼠肠道损伤的影响。方法将60只SD大鼠随机分为正常组(Normal组)、模型组(Model组)、低剂量RZ组(RZ-L组,3mg/kg RZ)、高剂量RZ组(RZ-H组,6mg/kg RZ)和高剂量RZ+Notch-1激活剂Jagged1组(RZ-H+Jagged1组,6mg/kg RZ+0.67mg/kg Jagged1),每组12只。在大鼠背部建立20%总体表面积(TBSA)Ⅲ度烫伤(烧伤)模型,之后注射铜绿假单胞菌制备烧伤脓毒症模型。HE染色观察回肠组织病理变化,并进行肠黏膜损伤评分(Chiu’s评分)。ELISA法检测血清内毒素、D-乳酸和二胺氧化酶(DAO)及回肠组织白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)、髓过氧化物酶(MPO)的水平。RT-qPCR法检测回肠组织Notch-1,Hes mRNA表达。Western Blot检测回肠组织Notch-1/Hes信号通路相关蛋白表达。结果与Normal组相比,Model组大鼠肠黏膜损伤评分(5.30±0.48分vs 1.00±0.52分)、血清内毒素(135.69±16.24 pg/ml vs 62.48±3.23 pg/ml),D-乳酸(1403.58±68.97 pg/ml vs 752.06±25.29 pg/ml)和DAO水平(137.55±12.37 pg/ml vs 58.52±4.43 pg/ml)、回肠组织IL-6(186.45±16.37 ng/ml vs 21.32±3.66 ng/ml),TNF-α(163.37±11.40 ng/ml vs 15.52±1.03 ng/ml)和MPO水平(8.94±0.92 U/ml vs 2.35±0.33 U/ml)及Notch-1 mRNA(1.86±0.19 vs 1.00±0.10)和蛋白(0.79±0.08 vs 0.21±0.02),Hes-1 mRNA(2.13±0.21 vs 1.00±0.12)和蛋白(0.88±0.09 vs 0.32±0.03)表达水平均升高,差异具有统计学意义(t=14.294~47.368,均P<0.05)。与Model组相比,RZ-L组和RZ-H组相应指标变化与上述相反,差异具有统计学意义(tRZ-L=5.650~21.448,tRZ-H=4.435~42.403,均P<0.05)。Notch-1激活剂Jagged1减弱了RZ对烧伤脓毒症大鼠肠道损伤的治疗作用。结论RZ可能通过抑制Notch-1/Hes信号通路减轻烧伤脓毒症模型大鼠的肠道损伤。
基金funded by the Natural Science Foundation of Hubei Province in China,No.02.02.040458
文摘Temporal lobe epilepsy is associated with astrogliosis. Notchl signaling can induce astrogliosis in glioma. However, it remains unknown whether Notchl signaling is involved in the pathogenesis of epilepsy. This study investigated the presence of Notchl, hairy and enhancer of split-l, and glial fibrillary acidic protein in the temporal neocortex and hippocampus of lithium-pilocar- pine-treated rats. The presence of Notchl and hairy and enhancer of split-1 was also explored in brain tissues of patients with intractable temporal lobe epilepsy. Quantitative electroencephalo- gram analysis and behavioral observations were used as auxiliary measures. Results revealed that the presence of Notchl, hairy and enhancer of split-l, and glial fibriUary acidic protein were en- hanced in status epilepticus and vehicle-treated spontaneous recurrent seizures rats, but remain unchanged in the following groups： control, absence of either status epilepticus or spontaneous recurrent seizures, and zileuton-treated spontaneous recurrent seizures. Compared with patient control cases, the presences of Notch1 and hairy and enhancer of split- 1 were upregulated in the temporal neocortex of patients with intractable temporal lobe epilepsy. Therefore, these results suggest that Notchl signaling may play an important role in the onset of temporal lobe epilepsy via astrogliosis. Furthermore, zileuton may be a potential therapeutic strategy for temporal lobe epilepsy by blocking Notchl signaling.
文摘目的观察骶1节段多裂肌劈开入路微创经锥间孔腰椎锥间融合术(MIS-TILF)治疗腰椎滑脱症的临床疗效。方法选择2017年2月-2018年2月期间在我院接受治疗的腰椎滑脱症患者98例,按照随机数字表法分为对照组和观察组,每组各49例。给予对照组患者常规正中骶棘肌剥离入路MIS-TILF治疗,给予观察组患者骶1节段多裂肌劈开入路MIS-TILF治疗。比较两组患者治疗前后腰椎功能、术后疼痛情况以及术中出血量、手术时间、术后引流量等手术相关指标。结果两组患者术前的ODI、JOA评分差异无统计学意义(P>0.05);术后,观察组患者ODI评分低于对照组,JOA评分高于对照组,差异具有统计学意义(P<0.05);术前,两组患者的疼痛VAS评分差异无统计学意义(P> 0.05);观察组患者术后3 d、14 d VAS评分均低于对照组,差异有统计学意义(P<0.05);观察组患者术中出血量、手术时间及术后引流量低于对照组,住院时间短于对照组,差异有统计学意义(P<0.05)。结论与传统正中骶棘肌剥离入路MIS-TILF治疗比较,骶1节段多裂肌劈开入路MIS-TILF在腰椎滑脱症的治疗中可有效促进患者腰椎功能恢复,同时具有术后疼痛小、术中出血少、术后恢复快等优势。
