To better understand the effect of a new split variant of human asialoglycoprotein receptor (ASGPR H1b) on ASGPR ligands’ binding ability, we established a functional cell line which expresses ASGPR.The full lengths ...To better understand the effect of a new split variant of human asialoglycoprotein receptor (ASGPR H1b) on ASGPR ligands’ binding ability, we established a functional cell line which expresses ASGPR.The full lengths of ASGPRH1a and H2c fragments from human liver were amplified by reverse transcript PCR (RT-PCR) and inserted into eukaryotic expression vector pIRES2EFP, pCDNA3.1 (Zeo+) respectively.The recombinants were cotransfected into HeLa cells.After selection by using Neocin and Zeocin, a stably transfected cell line was established, which was designated 4-1-6.The transcription and expression of ASGPRH1a and H2c in 4-1-6 were confirmed by RT-PCR, Western blotting and immunofluorescence.The endocytosis function of the artificial "ASGPR" on the surface of 4-1-6 was tested by FACS.It was found that the cell line 4-1-6 could bind ASGPR natural ligand molecular asialo-orosomucoid (ASOR).After the eukaryotic plasmid H1b/pCDNA3.1 (neo) was transfected into cell line 4-1-6, H1b did not down-regulate the ligand binding ability of ASGPR.The eukaryotic expression plasmid H1b/pcDNA3.1 (neo) and H2c/pcDNA3.1 (neo) were co-transfected transiently into Hela cell.Neither single H1b nor H1b and H2c could bind ASOR.In conclusion, a functional cell line of human asialoglycoprotein receptor (ASGPR) which expresses both H1a and H2c stably was established.The new split variant H1b has no effect on ASGPR binding to ASOR.ASGPRH1b alone can’t bind to ASOR, it yet can’t form functional complex with ASGPRH2c.展开更多
Methanol to aromatics produces multiple products,resulting in a limited selectivity of xylene.Aromatics upgrading is an effective way to produce more valuable xylene product,and different feed ratios generate discrepa...Methanol to aromatics produces multiple products,resulting in a limited selectivity of xylene.Aromatics upgrading is an effective way to produce more valuable xylene product,and different feed ratios generate discrepant product distributions.This work integrates the aromatics separation with toluene disproportionation,transalkylation of toluene and trimethylbenzene,and isomerization of xylene and trimethylbenzene.Exergy and exergoeconomic analyses are conducted to give insights in the splitting ratios of benzene,toluene and heavy aromatics for aromatics upgrading.First,a detailed simulation model is developed in Aspen HYSYS.Then,300 splitting ratio sets of benzene and toluene for conversion are studied to investigate the process performances.The results indicate that there are different preferences for the splitting ratios of benzene and toluene in terms of exergy and exergoeconomic performances.The process generates lower total exergy destruction when the splitting ratio of toluene varies between 0.07 and 0.18,and that of benzene fluctuates between 0.55 and 0.6.Nevertheless,the process presents lower total product unit cost with the splitting ratio of toluene less than 0.18 and that of benzene fluctuating between 0.44 and 0.89.Besides,it is found that distillation is the biggest contributor to the total exergy destruction,accounting for 94.97%.展开更多
基金supported by grants from the National Major Science and Technology Special Project for Infectious Diseases of China (No.2008ZX10002-011)National High Technology Research and Development of China (Program 863) (No.2006AA02Z128)the National Natural Science Foundation of China (Nos.30700701,30571646)
文摘To better understand the effect of a new split variant of human asialoglycoprotein receptor (ASGPR H1b) on ASGPR ligands’ binding ability, we established a functional cell line which expresses ASGPR.The full lengths of ASGPRH1a and H2c fragments from human liver were amplified by reverse transcript PCR (RT-PCR) and inserted into eukaryotic expression vector pIRES2EFP, pCDNA3.1 (Zeo+) respectively.The recombinants were cotransfected into HeLa cells.After selection by using Neocin and Zeocin, a stably transfected cell line was established, which was designated 4-1-6.The transcription and expression of ASGPRH1a and H2c in 4-1-6 were confirmed by RT-PCR, Western blotting and immunofluorescence.The endocytosis function of the artificial "ASGPR" on the surface of 4-1-6 was tested by FACS.It was found that the cell line 4-1-6 could bind ASGPR natural ligand molecular asialo-orosomucoid (ASOR).After the eukaryotic plasmid H1b/pCDNA3.1 (neo) was transfected into cell line 4-1-6, H1b did not down-regulate the ligand binding ability of ASGPR.The eukaryotic expression plasmid H1b/pcDNA3.1 (neo) and H2c/pcDNA3.1 (neo) were co-transfected transiently into Hela cell.Neither single H1b nor H1b and H2c could bind ASOR.In conclusion, a functional cell line of human asialoglycoprotein receptor (ASGPR) which expresses both H1a and H2c stably was established.The new split variant H1b has no effect on ASGPR binding to ASOR.ASGPRH1b alone can’t bind to ASOR, it yet can’t form functional complex with ASGPRH2c.
基金the National Natural Science Foundation of China(Grant No.21736008)the National Key Research and Development Program of China(Grant No.2018YFB0604803).
文摘Methanol to aromatics produces multiple products,resulting in a limited selectivity of xylene.Aromatics upgrading is an effective way to produce more valuable xylene product,and different feed ratios generate discrepant product distributions.This work integrates the aromatics separation with toluene disproportionation,transalkylation of toluene and trimethylbenzene,and isomerization of xylene and trimethylbenzene.Exergy and exergoeconomic analyses are conducted to give insights in the splitting ratios of benzene,toluene and heavy aromatics for aromatics upgrading.First,a detailed simulation model is developed in Aspen HYSYS.Then,300 splitting ratio sets of benzene and toluene for conversion are studied to investigate the process performances.The results indicate that there are different preferences for the splitting ratios of benzene and toluene in terms of exergy and exergoeconomic performances.The process generates lower total exergy destruction when the splitting ratio of toluene varies between 0.07 and 0.18,and that of benzene fluctuates between 0.55 and 0.6.Nevertheless,the process presents lower total product unit cost with the splitting ratio of toluene less than 0.18 and that of benzene fluctuating between 0.44 and 0.89.Besides,it is found that distillation is the biggest contributor to the total exergy destruction,accounting for 94.97%.
