Fruit spine is an important quality trait of cucumber.To better understand the molecular basis of cucumber spine development and function,RNA-Seq was performed to identify differentially expressed genes(DEGs)in fruit ...Fruit spine is an important quality trait of cucumber.To better understand the molecular basis of cucumber spine development and function,RNA-Seq was performed to identify differentially expressed genes(DEGs)in fruit spines of different development stages,namely,8 days before anthesis(SpBA8),anthesis(SpA)and 8 days after anthesis(SpAA8).Stage-wise comparisons obtained 2,259(SpBA8 vs.SpA),4,551(SpA vs.SpAA8),and 5,290(SpBA8 vs.SpAA8)DEGs.All the DEGs were classified into eight expression clusters by trend analysis.Among these DEGs,in addition to the Mict,Tril,CsTTG1,CsMYB6,NS,and Tu genes that have been reported to regulate fruit spine formation,we found that the CsHDG11,CsSCL8,CsSPL8,CsZFP6 and CsZFP8 may also be involved in spine development in cucumber.Our study provides a theoretical basis for further research on molecular mechanisms of spine development in cucumber.展开更多
Introduction-The cervical spine is subjected to injury frequently,especially among pilots who are usually on the condition of high acceleration.Injuries of the cervical spine will be potential risk of damage to the sp...Introduction-The cervical spine is subjected to injury frequently,especially among pilots who are usually on the condition of high acceleration.Injuries of the cervical spine will be potential risk of damage to the spinal cord,which could be result in life threatening展开更多
目的:探讨发育性髋关节发育不良(developmental dysplasia of the hip,DDH)患者进行全髋关节置换术(total hip arthroplasty,THA)后脊柱冠状位序列变化的影响。方法:纳入2021年3月至2023年12月我院进行全髋置换术临床资料完整的DDH患者2...目的:探讨发育性髋关节发育不良(developmental dysplasia of the hip,DDH)患者进行全髋关节置换术(total hip arthroplasty,THA)后脊柱冠状位序列变化的影响。方法:纳入2021年3月至2023年12月我院进行全髋置换术临床资料完整的DDH患者22例,男9例、女13例;平均年龄54.23±10.07岁。左侧19例,右侧3例。记录术前和术后站立位脊柱全长正侧位X线片及站立位下肢全长正侧位X线片,测量脊柱-骨盆冠状位参数:Cobb角、下肢长度、骶骨倾斜角、髂骨倾斜角、腰5(L5)倾斜角、CE角。分析冠状位脊柱-骨盆参数之间的相关性。结果:全髋关节置换术前下肢长度为83.77±4.33cm,术后下肢长度为84.02±1.01cm,差异无统计学意义(P>0.05);术前术后的下肢对比健侧肢体的长度差值的差异有统计学意义(P<0.05);骶骨倾斜角由术前4.38±5.62度至4.62±5.43度,差异无统计学意义(P>0.05);髂骨倾斜角术前为58.19±3.20度,术后为51.00±3.89度,差异有统计学意义(P<0.05);术前CE角度为19.34±7.17度,术后角度为56.21±9.10度,差异有统计学意义(P<0.05);Cobb角位5.70±4.34度,术后为6.03±3.65度,差异无统计学意义(P>0.05);L5倾斜角术前为2.75±1.93度,术后为2.47±1.91度,差异无统计学意义(P>0.05)。骶骨倾斜角与髂骨倾斜角,L5倾斜角存在相关性(r=0.462,P=0.030;r=0.437,P=0.042)。结论:对于DDH患者在经过人工全髋置换术后肢体长度的差异得到改善,骨盆的冠状位倾斜得到一定矫正,下腰部的力学形态得到改善,可能对于下腰痛有一定的缓解。展开更多
目的:比较正常人和髋关节发育不良(developmental dysplasia of the hip,DDH)患者日常活动下脊柱-髋关节的应力分布、位移与矢状面参数。方法:依据1例24岁男性DDH患者(身高175 cm,体质量65 kg)与1名健康男性志愿者的CT图像,采用骨科通...目的:比较正常人和髋关节发育不良(developmental dysplasia of the hip,DDH)患者日常活动下脊柱-髋关节的应力分布、位移与矢状面参数。方法:依据1例24岁男性DDH患者(身高175 cm,体质量65 kg)与1名健康男性志愿者的CT图像,采用骨科通用方法重建两者的髋关节和脊柱计算机辅助设计(computer aided design,CAD)模型,建立其脊柱-髋关节耦合有限元模型,预测在站立和坐下位姿、步行和站立-坐下运动时的脊柱和髋关节应力位移和骨盆相对腰椎的位姿变化。结果:预测所得的正常人在脊柱屈伸、侧弯和旋转时,除L_(2)-L_(3)椎间侧弯角度(5.5°)较既往研究的最小侧弯角度(7.5°)小之外,其余椎间各活动度均在既往研究的范围内,间接证实了有限元模型的有效性。预测所得腰椎间盘最大应力(1.6 MPa)出现在L_(5)-S(S为骶骨)的椎间盘,而最小应力(0.93 MPa)出现在L_(1)-L_(2)的椎间盘,且应力分布从上到下逐渐增大。静态站立和坐位时,DDH患者脊柱均出现明显应力集中且最大应力大于正常人;步态运动过程中,DDH患者脊柱最大应力(约279.68 MPa)明显高于正常人(255.57 MPa),但双侧骨盆位移低于正常人;站立-坐下运动过程中,DDH患者较正常人的髋关节应力和位移呈现与步行相同的趋势。两种运动均表明DDH患者的脊柱-髋关节活动度低于正常人且微观力学性能退变。结论:本研究初步建立了人体脊柱-髋关节耦合有限元模型,并预测了DDH患者相比正常人其脊柱和髋关节的微观力学性能和腰髋联动特性,为临床病因诊断提供新的生物力学方法,为深度探究脊柱-髋关节的生物力学提供了新方法和初步理论参考。展开更多
目的探讨发育性髋关节发育不良(developmental dysplasia of the hip,DDH)患者脊柱-骨盆冠状位序列的变化规律以及全髋关节置换术对冠状位脊柱-骨盆序列的影响。方法纳入临床资料完整的DDH患者48例,男6例、女42例;年龄(42.81±10.42...目的探讨发育性髋关节发育不良(developmental dysplasia of the hip,DDH)患者脊柱-骨盆冠状位序列的变化规律以及全髋关节置换术对冠状位脊柱-骨盆序列的影响。方法纳入临床资料完整的DDH患者48例,男6例、女42例;年龄(42.81±10.42)岁(范围22~61岁)。双侧髋发育不良11例,单侧髋发育不良37例,共59髋。Crowe分型Ⅰ型13髋、Ⅱ型7髋、Ⅲ型4髋、Ⅳ型35髋。于术前和末次随访时摄站立位脊柱全长正位X线片及站立位下肢全长正位X线片,测量脊柱-骨盆冠状位参数:Cobb角、冠状位平衡、骶骨倾斜角、髂骨倾斜角、髋关节倾斜角、L5倾斜角、下肢长度。分析冠状位脊柱-骨盆参数之间的相关性,评估数据测量的可重复性。结果随访时间(10.31±2.62)个月(范围3~18个月)。DDH患者术前髂骨倾斜角、骶骨倾斜角、髋关节倾斜角和L5倾斜角的观察者内复测信度分别为0.965、0.875、0.912和0.934,观察者间信度分别为0.887、0.889、0.892和0.907。33例DDH患者存在双下肢不等长,发生率为69%(33/48);28例患者伴有脊柱侧凸,发生率为58%(28/48);发生髋关节脱位43例,其中37例股骨头相对位置较高一侧(即脱位侧)的骨盆位置更低,发生率86%(37/43);发生骨盆倾斜46例,发生率为96%(46/48)。全髋关节置换术后髂骨倾斜角由术前6.71°±4.62°降低至3.52°±3.14°,差异有统计学意义(t=4.81,P<0.001);骶骨倾斜角由术前6.38°±5.48°降低至3.72°±3.38°,差异有统计学意义(t=3.91,P<0.001);术前髋关节倾斜角为5.32°±5.83°、术后为3.71°±3.62°,差异无统计学意义(t=1.85,P=0.071);L5倾斜角由术前6.12°±5.46°降低至4.33°±4.71°,差异有统计学意义(t=2.15,P=0.037);Cobb角由术前11.05°±10.76°降低至6.82°±7.76°,差异有统计学意义(t=4.07,P<0.001);术前冠状位平衡为(14.22±10.64)mm、术后为(13.73±12.58)mm,差异无统计学意义(t=0.24,P=0.821)。Cobb角与骶骨倾斜角、髂骨倾斜角均呈正相关(r=0.61,P<0.001;r=0.57,P<0.001)。结论DDH患者出现骨盆冠状位倾斜的原因包括双下肢不等长与髋关节脱位。冠状位骨盆倾斜导致代偿性脊柱侧凸,使整体冠状位脊柱-骨盆序列发生改变,但仍能保持冠状位平衡。全髋关节置换术能够显著改善DDH患者冠状位骨盆倾斜以及代偿性侧凸的程度。展开更多
基金sponsored by the Natural Science Foundation of Shanghai(20ZR1439600,19ZR1436500)the Agricultural Field Project of Shanghai“Action Plan for Scientific and Technological Innovation”(20392001300)+1 种基金the Young and Middle-Aged Leading Talent Project of Shanghai Vocational College of Agriculture and Forestry(A2-0273-20-01-16)the Project of Shanghai Vocational College of Agriculture and Forestry(KY2-0000-20-01).
文摘Fruit spine is an important quality trait of cucumber.To better understand the molecular basis of cucumber spine development and function,RNA-Seq was performed to identify differentially expressed genes(DEGs)in fruit spines of different development stages,namely,8 days before anthesis(SpBA8),anthesis(SpA)and 8 days after anthesis(SpAA8).Stage-wise comparisons obtained 2,259(SpBA8 vs.SpA),4,551(SpA vs.SpAA8),and 5,290(SpBA8 vs.SpAA8)DEGs.All the DEGs were classified into eight expression clusters by trend analysis.Among these DEGs,in addition to the Mict,Tril,CsTTG1,CsMYB6,NS,and Tu genes that have been reported to regulate fruit spine formation,we found that the CsHDG11,CsSCL8,CsSPL8,CsZFP6 and CsZFP8 may also be involved in spine development in cucumber.Our study provides a theoretical basis for further research on molecular mechanisms of spine development in cucumber.
文摘Introduction-The cervical spine is subjected to injury frequently,especially among pilots who are usually on the condition of high acceleration.Injuries of the cervical spine will be potential risk of damage to the spinal cord,which could be result in life threatening
文摘目的:探讨发育性髋关节发育不良(developmental dysplasia of the hip,DDH)患者进行全髋关节置换术(total hip arthroplasty,THA)后脊柱冠状位序列变化的影响。方法:纳入2021年3月至2023年12月我院进行全髋置换术临床资料完整的DDH患者22例,男9例、女13例;平均年龄54.23±10.07岁。左侧19例,右侧3例。记录术前和术后站立位脊柱全长正侧位X线片及站立位下肢全长正侧位X线片,测量脊柱-骨盆冠状位参数:Cobb角、下肢长度、骶骨倾斜角、髂骨倾斜角、腰5(L5)倾斜角、CE角。分析冠状位脊柱-骨盆参数之间的相关性。结果:全髋关节置换术前下肢长度为83.77±4.33cm,术后下肢长度为84.02±1.01cm,差异无统计学意义(P>0.05);术前术后的下肢对比健侧肢体的长度差值的差异有统计学意义(P<0.05);骶骨倾斜角由术前4.38±5.62度至4.62±5.43度,差异无统计学意义(P>0.05);髂骨倾斜角术前为58.19±3.20度,术后为51.00±3.89度,差异有统计学意义(P<0.05);术前CE角度为19.34±7.17度,术后角度为56.21±9.10度,差异有统计学意义(P<0.05);Cobb角位5.70±4.34度,术后为6.03±3.65度,差异无统计学意义(P>0.05);L5倾斜角术前为2.75±1.93度,术后为2.47±1.91度,差异无统计学意义(P>0.05)。骶骨倾斜角与髂骨倾斜角,L5倾斜角存在相关性(r=0.462,P=0.030;r=0.437,P=0.042)。结论:对于DDH患者在经过人工全髋置换术后肢体长度的差异得到改善,骨盆的冠状位倾斜得到一定矫正,下腰部的力学形态得到改善,可能对于下腰痛有一定的缓解。
文摘目的探讨发育性髋关节发育不良(developmental dysplasia of the hip,DDH)患者脊柱-骨盆冠状位序列的变化规律以及全髋关节置换术对冠状位脊柱-骨盆序列的影响。方法纳入临床资料完整的DDH患者48例,男6例、女42例;年龄(42.81±10.42)岁(范围22~61岁)。双侧髋发育不良11例,单侧髋发育不良37例,共59髋。Crowe分型Ⅰ型13髋、Ⅱ型7髋、Ⅲ型4髋、Ⅳ型35髋。于术前和末次随访时摄站立位脊柱全长正位X线片及站立位下肢全长正位X线片,测量脊柱-骨盆冠状位参数:Cobb角、冠状位平衡、骶骨倾斜角、髂骨倾斜角、髋关节倾斜角、L5倾斜角、下肢长度。分析冠状位脊柱-骨盆参数之间的相关性,评估数据测量的可重复性。结果随访时间(10.31±2.62)个月(范围3~18个月)。DDH患者术前髂骨倾斜角、骶骨倾斜角、髋关节倾斜角和L5倾斜角的观察者内复测信度分别为0.965、0.875、0.912和0.934,观察者间信度分别为0.887、0.889、0.892和0.907。33例DDH患者存在双下肢不等长,发生率为69%(33/48);28例患者伴有脊柱侧凸,发生率为58%(28/48);发生髋关节脱位43例,其中37例股骨头相对位置较高一侧(即脱位侧)的骨盆位置更低,发生率86%(37/43);发生骨盆倾斜46例,发生率为96%(46/48)。全髋关节置换术后髂骨倾斜角由术前6.71°±4.62°降低至3.52°±3.14°,差异有统计学意义(t=4.81,P<0.001);骶骨倾斜角由术前6.38°±5.48°降低至3.72°±3.38°,差异有统计学意义(t=3.91,P<0.001);术前髋关节倾斜角为5.32°±5.83°、术后为3.71°±3.62°,差异无统计学意义(t=1.85,P=0.071);L5倾斜角由术前6.12°±5.46°降低至4.33°±4.71°,差异有统计学意义(t=2.15,P=0.037);Cobb角由术前11.05°±10.76°降低至6.82°±7.76°,差异有统计学意义(t=4.07,P<0.001);术前冠状位平衡为(14.22±10.64)mm、术后为(13.73±12.58)mm,差异无统计学意义(t=0.24,P=0.821)。Cobb角与骶骨倾斜角、髂骨倾斜角均呈正相关(r=0.61,P<0.001;r=0.57,P<0.001)。结论DDH患者出现骨盆冠状位倾斜的原因包括双下肢不等长与髋关节脱位。冠状位骨盆倾斜导致代偿性脊柱侧凸,使整体冠状位脊柱-骨盆序列发生改变,但仍能保持冠状位平衡。全髋关节置换术能够显著改善DDH患者冠状位骨盆倾斜以及代偿性侧凸的程度。
