13′—cis Spheroidene在己烷中(浓度3.00×10^(-5)mol/l),在170K的温度下,测定吸收光谱、荧光发射谱和荧光激发谱。确定了分子的能级S_3、S_4和cis峰能级,分别为20600、26900和28700cm^(-1)(示出数据为基态和各能级间的(0←0)跃迁)...13′—cis Spheroidene在己烷中(浓度3.00×10^(-5)mol/l),在170K的温度下,测定吸收光谱、荧光发射谱和荧光激发谱。确定了分子的能级S_3、S_4和cis峰能级,分别为20600、26900和28700cm^(-1)(示出数据为基态和各能级间的(0←0)跃迁)。13′—cis分子的光谱特征和它的异构体all—trans S pheroidene的相应谱线比较,给出了一些有益的提示。展开更多
All-trans spheroidene was extracted from the cells of Rhodobacer sphaeroides 2.4.1 and a set of cis-trans isomers were isolated from an isomeric mixture obtained by iodine-sensitized photo-isomerization of the all-tra...All-trans spheroidene was extracted from the cells of Rhodobacer sphaeroides 2.4.1 and a set of cis-trans isomers were isolated from an isomeric mixture obtained by iodine-sensitized photo-isomerization of the all-trans isomer by high-pressure liquid chromatography (HPLC). On the basis of assignment of the 1H-NMR spectra (COSY, long-range COSY, rekyed COSY, ROESY and NOESY) of the all-trans isomer, the configurations of cis isomers were determined by the isomerization shifts of the olefinic 1H signals. The peaks of HPLC could be assigned as follows: A: 9,13’-cis, B1: 5,13-cis, B2: 13,9’-cis, C: 13-cis, E: 9-cis, F: 13’-cis, G: 5,9’-cis, H: 9’-cis, I: all-trans.展开更多
文摘13′—cis Spheroidene在己烷中(浓度3.00×10^(-5)mol/l),在170K的温度下,测定吸收光谱、荧光发射谱和荧光激发谱。确定了分子的能级S_3、S_4和cis峰能级,分别为20600、26900和28700cm^(-1)(示出数据为基态和各能级间的(0←0)跃迁)。13′—cis分子的光谱特征和它的异构体all—trans S pheroidene的相应谱线比较,给出了一些有益的提示。
文摘All-trans spheroidene was extracted from the cells of Rhodobacer sphaeroides 2.4.1 and a set of cis-trans isomers were isolated from an isomeric mixture obtained by iodine-sensitized photo-isomerization of the all-trans isomer by high-pressure liquid chromatography (HPLC). On the basis of assignment of the 1H-NMR spectra (COSY, long-range COSY, rekyed COSY, ROESY and NOESY) of the all-trans isomer, the configurations of cis isomers were determined by the isomerization shifts of the olefinic 1H signals. The peaks of HPLC could be assigned as follows: A: 9,13’-cis, B1: 5,13-cis, B2: 13,9’-cis, C: 13-cis, E: 9-cis, F: 13’-cis, G: 5,9’-cis, H: 9’-cis, I: all-trans.
