In this paper, separation of Sr from associated elements （Na, K, Ca, Mg, and Ba） was carried out using an extraction chromatographic column packed with Sr selective specific resin consisting of an octanol solution o...In this paper, separation of Sr from associated elements （Na, K, Ca, Mg, and Ba） was carried out using an extraction chromatographic column packed with Sr selective specific resin consisting of an octanol solution of 4,4＇（5＇）-bis（t-butylcyclohexo）-lS-crown-6 sorbed on an inert polymeric support. Using 8.0 mol/L HNO3 as the eluent, Ba and associated elements were eluted immediately from the column, whereas Sr was strongly retained. The adsorbed Sr could then be stripped from the column as the eluent was changed to 0.05 mol/L HNO~. Complete Sr/Ba separation was demonstrated to be feasible in that Ba could be washed from the column leaving pure Sr by adequate rinsing with 8.0 mol/L HN03. Furthermore, matrix effect could be effectively eliminated and the selectivity of the method was improved. The method was applied for the determination of Sr in the high purity barium carbonate （BaCO3） product and seawater. The recoveries of Sr in 2 samples were determined to be 93.2% and 109%, respectively.展开更多
To address critical challenges of protein template denaturation caused by intense exothermicity and prolonged reaction time when using the traditional protein molecular imprinting technology,a novel imprinting strateg...To address critical challenges of protein template denaturation caused by intense exothermicity and prolonged reaction time when using the traditional protein molecular imprinting technology,a novel imprinting strategy was proposed.This study successfully achieved the rapid and controllable in-situ synthesis of polyacrylamide/calcium alginate(PAM/CaAlg)hydrogel films under near-ambient temperature conditions,employing a silver ions(Ag^(+))-catalyzed ammonium persulfate-sodium bisulfite redox system with acrylamide(AM)as the monomer,N,N′-methylenebisacrylamide(MBA)as the crosslinker,and bovine serum albumin(BSA)as the template.The optimized molecularly imprinted polymer(MIP)films demonstrated substantial enhancement of the BSA adsorption capacity following the removal of templates,reaching a maximum equilibrium adsorption capacity(Qe)of 50.4 mg·g-1 while maintaining a stable imprinting efficiency(IE)of 2.7.Competitive adsorption experiments verified the exceptional selectivity of MIP films towards the BSA recognition.Additionally,the incorporation of Ag^(+)ions endowed both MIP and nonimprinted polymer(NIP)films with remarkable antibacterial properties.This work establishes a straightforward and effective methodology for developing advanced protein-imprinted hydrogels that simultaneously exhibit high adsorption capacity,superior selectivity,and significant antibacterial activity.展开更多
基金supported by Public Welfare Scientific Research Project of the Ministry of Land and Resource of China(Nos.200911044 and 201111028)the Fund of Key Laboratory of Global Change and Marine-Atmospheric Chemistry, State Oceanic Administration(No.GCMAC1206)
文摘In this paper, separation of Sr from associated elements （Na, K, Ca, Mg, and Ba） was carried out using an extraction chromatographic column packed with Sr selective specific resin consisting of an octanol solution of 4,4＇（5＇）-bis（t-butylcyclohexo）-lS-crown-6 sorbed on an inert polymeric support. Using 8.0 mol/L HNO3 as the eluent, Ba and associated elements were eluted immediately from the column, whereas Sr was strongly retained. The adsorbed Sr could then be stripped from the column as the eluent was changed to 0.05 mol/L HNO~. Complete Sr/Ba separation was demonstrated to be feasible in that Ba could be washed from the column leaving pure Sr by adequate rinsing with 8.0 mol/L HN03. Furthermore, matrix effect could be effectively eliminated and the selectivity of the method was improved. The method was applied for the determination of Sr in the high purity barium carbonate （BaCO3） product and seawater. The recoveries of Sr in 2 samples were determined to be 93.2% and 109%, respectively.
基金supported by the project of open bidding for selecting the best candidates in Tianjin Institute of Acupuncture and Moxibustion,and the National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion(ZJJBGS2024002-2).
文摘To address critical challenges of protein template denaturation caused by intense exothermicity and prolonged reaction time when using the traditional protein molecular imprinting technology,a novel imprinting strategy was proposed.This study successfully achieved the rapid and controllable in-situ synthesis of polyacrylamide/calcium alginate(PAM/CaAlg)hydrogel films under near-ambient temperature conditions,employing a silver ions(Ag^(+))-catalyzed ammonium persulfate-sodium bisulfite redox system with acrylamide(AM)as the monomer,N,N′-methylenebisacrylamide(MBA)as the crosslinker,and bovine serum albumin(BSA)as the template.The optimized molecularly imprinted polymer(MIP)films demonstrated substantial enhancement of the BSA adsorption capacity following the removal of templates,reaching a maximum equilibrium adsorption capacity(Qe)of 50.4 mg·g-1 while maintaining a stable imprinting efficiency(IE)of 2.7.Competitive adsorption experiments verified the exceptional selectivity of MIP films towards the BSA recognition.Additionally,the incorporation of Ag^(+)ions endowed both MIP and nonimprinted polymer(NIP)films with remarkable antibacterial properties.This work establishes a straightforward and effective methodology for developing advanced protein-imprinted hydrogels that simultaneously exhibit high adsorption capacity,superior selectivity,and significant antibacterial activity.
