Discriminating sterile inflammation from infection, especially in cases of aseptic loosening versus an actual prosthetic joint infection, is challenging and has significant treatment implications. Our goal was to eval...Discriminating sterile inflammation from infection, especially in cases of aseptic loosening versus an actual prosthetic joint infection, is challenging and has significant treatment implications. Our goal was to evaluate a novel human monoclonal antibody（mAb） probe directed against the Gram-positive bacterial surface molecule lipoteichoic acid（LTA）. Specificity and affinity were assessed in vitro. We then radiolabeled the anti-LTA mAb and evaluated its effectiveness as a diagnostic imaging tool for detecting infection via immuno PET imaging in an in vivo mouse model of prosthetic joint infection（PJI）. In vitro and ex vivo binding of the anti-LTA mAb to pathogenic bacteria was measured with Octet, ELISA, and flow cytometry. The in vivo PJI mouse model was assessed using traditional imaging modalities, including positron emission tomography（PET） with [^（18）F]FDG and [^（18）F]Na F as well as X-ray computed tomography（CT）, before being evaluated with the zirconium-89-labeled antibody specific for LTA（[^（89）Zr]SAC55）.The anti-LTA mAb exhibited specific binding in vitro to LTA-expressing bacteria. Results from imaging showed that our model could reliably simulate infection at the surgical site by bioluminescent imaging, conventional PET tracer imaging, and bone morphological changes by CT. One day following injection of both the radiolabeled anti-LTA and isotype control antibodies, the anti-LTA antibody demonstrated significantly greater（P 〈 0.05） uptake at S. aureus-infected prosthesis sites over either the same antibody at sterile prosthesis sites or of control non-specific antibody at infected prosthesis sites. Taken together, the radiolabeled anti-LTA mAb, [^（89）Zr]SAC55, may serve as a valuable diagnostic molecular imaging probe to help distinguish between sterile inflammation and infection in the setting of PJI. Future studies are needed to determine whether these findings will translate to human PJI.展开更多
Increasingly serious microbial infections call for the development of new simpler methods for the precise diagnosis and specific inhibition of such pathogens. In this work, a peptide mineralized Au cluster probe was a...Increasingly serious microbial infections call for the development of new simpler methods for the precise diagnosis and specific inhibition of such pathogens. In this work, a peptide mineralized Au cluster probe was applied as a new simplified strategy to both recognize and inhibit a single bacteria species of Staphylococcus aureus(S. aureus) simultaneously. The probes are composed of peptides and Au clusters. Moreover, the peptides specifically target S. aureus cells and the Au clusters provide fluorescent imaging and have an antibacterial effect. These new probes enable the simultaneous specific detection and effective destruction S. aureus cells in situ.展开更多
A functionalized silicon nanowire field-effect transistor （SiNW FET） was fabricated to detect single molecules in the pM range to detect disease at the early stage with a sensitive, robust, and inexpensive method wi...A functionalized silicon nanowire field-effect transistor （SiNW FET） was fabricated to detect single molecules in the pM range to detect disease at the early stage with a sensitive, robust, and inexpensive method with the ability to provide specific and reliable data. The device was designed and fabricated by indented ash trimming via shallow anisotropic etching. The approach is a simple and low-cost technique that is compatible with the current commercial semiconductor standard CMOS process without an expensive deep reactive ion etcher. Specific electric changes were observed for DNA sensing when the nanowire surface was modified with a complementary captured DNA probe and target DNA through an organic linker （--OCH2CH3） using organofunctional alkoxysilanes （3-aminopropyl） triethoxysilane （APTES）. With this surface modification, a single specific target molecule can be detected. The simplicity of the sensing domain makes it feasible to miniaturize it for the development of a cancer detection kit, facilitating its use in both clinical and non-clinical environments to allow non-expert interpretation. With its novel electric response and potential for mass commercial fabrication, this biosensor can be developed to become a portable/point of care biosensor for both field and diagnostic applications.展开更多
Objective To determine the diagnostic significance of detecting the specific epithelial keratin CK-20 mRNA in peripheral venous blood from patients with bladder carcinomas. Methods Reverse transcription coupled with t...Objective To determine the diagnostic significance of detecting the specific epithelial keratin CK-20 mRNA in peripheral venous blood from patients with bladder carcinomas. Methods Reverse transcription coupled with two-step polymerase chain reaction (nested RT-PCR) was used to detect CK-20 mRNA expression in the peripheral blood from patients with blodder carcinomas. Results Detection of CK-20 mRNA expression was positive in 37 of 91 patients with bladder carcinoma (41 % ). Among 20 patients with distant metastasis, 17 were positive (85 % ). CK-20 mRNA was not detectable in the blood samples from 25 normal individuals. The frequency of positive CK-20 mRNA expression was signficantly higher in those with distant metastasis. Conclusion The presence of CK-20 mRNA expression in peripheral blood may be used as an early indicator of hematogenous metastasis of bladder carcinoma cells. 6 refs,1 tab.展开更多
Various novel conjugated polymers(CPs)have been developed for organic photodetectors(OPDs),but their application to practical image sensors such as X-ray,R/G/B,and fingerprint sensors is rare.In this article,we report...Various novel conjugated polymers(CPs)have been developed for organic photodetectors(OPDs),but their application to practical image sensors such as X-ray,R/G/B,and fingerprint sensors is rare.In this article,we report the entire process from the synthesis and molecular engineering of novel CPs to the development of OPDs and fingerprint image sensors.We synthesized six benzo[1,2-d:4,5-d’]bis(oxazole)(BBO)-based CPs by modifying the alkyl side chains of the CPs.Several relationships between the molecular structure and the OPD performance were revealed,and increasing the number of linear octyl side chains on the conjugated backbone was the best way to improve Jph and reduce Jd in the OPDs.The optimized CP demonstrated promising OPD performance with a responsivity(R)of 0.22 A/W,specific detectivity(D^(*))of 1.05×10^(13)Jones at a bias of-1 V,rising/falling response time of 2.9/6.9μs,and cut-off frequency(f_(-3dB))of 134 kHz under collimated 530 nm LED irradiation.Finally,a fingerprint image sensor was fabricated by stacking the POTB1-based OPD layer on the organic thin-film transistors(318 ppi).The image contrast caused by the valleys and ridges in the fingerprints was obtained as a digital signal.展开更多
The rapid development of modern infrared optoelectronic technology has driven a growing demand for high-sensitivity mid-wave infrared(MWIR)photodetectors capable of reliable room-temperature operation.Achieving optima...The rapid development of modern infrared optoelectronic technology has driven a growing demand for high-sensitivity mid-wave infrared(MWIR)photodetectors capable of reliable room-temperature operation.Achieving optimal specific detectivity,a critical performance metric for MWIR photodetection,remains challenging due to inherent limitations imposed such as high dark current,low optical absorption,or both.To address these challenges,we present an approach based on a bipolar-barrier architecture featuring a black phosphorus(BP)/MoTe_(2)/BP tunnel heterostructure integrated with an Au reflector.This configuration delivers simultaneous electrical and optical enhancement,effectively suppressing dark currents and significantly increasing optical absorption.The bipolar-barrier structure minimizes dark current by blocking thermally excited and bias-induced carrier leakage,while facilitating efficient tunneling of photogenerated carriers via trap-assisted photogating mechanisms.In addition,the Au reflector enhances optical absorption through interference effects.As a result,the heterostructure achieves remarkable performance metrics,including a room-temperature specific detectivity of~3.0×10^(10)cm Hz0.5 W^(-1),a high responsivity of~4 A W^(-1),and an external quantum efficiency of~140%within the MWIR range.These results establish the bipolar-barrier tunnel heterostructure as a highly efficient platform,paving the way for the next generation of advanced infrared optoelectronic devices.展开更多
Terahertz metamaterial biosensors combine terahertz time-domain spectroscopy with metamaterial sensing to provide a sensitive detection platform for a variety of targets,including biological molecules,proteins,cells,a...Terahertz metamaterial biosensors combine terahertz time-domain spectroscopy with metamaterial sensing to provide a sensitive detection platform for a variety of targets,including biological molecules,proteins,cells,and viruses.These biosensors are characterized by their rapid response,sensitivity,non-destructive,label-free operation,minimal sample requirement,and user-friendly design,which also allows for integration with various technical approaches.Advancing beyond traditional biosensors,terahertz metamaterial biosensors facilitate rapid and non-destructive trace detection in biomedical applications,contributing to timely diagnosis and early screening of diseases.In this paper,the theoretical basis and advanced progress of these biosensors are discussed in depth,focusing on three key areas:improving the sensitivity and specificity,and reducing the influence of water absorption in biological samples.This paper also analyzes the potential and future development of these biosensors for expanded applications.It highlights their potential for multi-band tuning,intelligent operations,and flexible,wearable biosensor applications.This review provides a valuable reference for the follow-up research and application of terahertz metamaterial biosensors in the field of biomedical detection.展开更多
Broad-spectrum photodetectors(PDs)are essential for various health monitoring,night vision,and telecommunications applications,but their detectivity in a wide absorbance region is limited by undesirable electronic res...Broad-spectrum photodetectors(PDs)are essential for various health monitoring,night vision,and telecommunications applications,but their detectivity in a wide absorbance region is limited by undesirable electronic response properties.Colloidal quantum dots(CQDs)are a promising system for broad-spectrum detection,whereas their practical potential is hindered by suboptimal dark current characteristics.To overcome these challenges,we propose a layered architecture comprising CQDs and a bulk heterojunction(BHJ)organic film as a hole transport layer.The integration of PbS CQDs offers multiple benefits,including bandgap tuning for minimizing thermal carriers,surface passivation to reduce recombination rates,and the formation of high-quality interfaces with organic layers,which collectively contribute to suppressing dark current leakage and thermal excitations by suppressing stray electrons.By integrating ITIC into the BHJ film,the device detectability is significantly enhanced,reaching 1013 Jones in the 400–1000 nm spectral range.This improvement is attributed to the higher lowest unoccupied molecular orbital(LUMO)of ITIC molecules,which effectively hinders electron injection.Additionally,J-aggregation-induced molecular stacking and optimized phase separation of BHJ films contribute to the enhanced performance.The integration of diverse materials offers greater flexibility in device design and functionality,enabling the development of more advanced and sophisticated optoelectronic devices.Furthermore,this approach could significantly enhance the theoretical and practical understanding of optoelectronic device engineering,leading to the development of more advanced optoelectronic devices.展开更多
Subject Code:H30With the support by the National Natural Science Foundation of China and National Basic Research Program of China,the group led by Prof.Ge Guangbo(葛广波)and Prof.Yang Ling(杨凌)from the Laboratory of ...Subject Code:H30With the support by the National Natural Science Foundation of China and National Basic Research Program of China,the group led by Prof.Ge Guangbo(葛广波)and Prof.Yang Ling(杨凌)from the Laboratory of Pharmaceutical Resource Discovery,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,reported a highly specific ratiometric two-photon fluorescent probe to detect展开更多
Organic-inorganic hybrid perovskites are ideal materials for photodetection owing to their high charge carrier mobility, long charge carrier diffusion length, low dark current density and sharp absorption edge. Howeve...Organic-inorganic hybrid perovskites are ideal materials for photodetection owing to their high charge carrier mobility, long charge carrier diffusion length, low dark current density and sharp absorption edge. However, a relatively small band gap(1.6 e V) limits their photonharvesting efficiency in the near-infrared region. In the present work, we demonstrate a hybrid methylamine iodide and Pb-Sn binary perovskite as the light absorption layer in photodetectors. Experimentally, the wavelength of photoresponse onset for the photodetectors can be extended to as great as 1,000 nm when the Sn content of the hybrid perovskite is increased to 30 mol%. In addition, the photodetectors exhibit a photoresponsivity of 0.39 A W^-1, a specific detectivity of 7×10^12 Jones, a fast photoresponse with rise and decay time constants and an external quantum efficiency greater than 50% in the wavelength range of350–900 nm, with a maximum value of about 80% at 550 nm.展开更多
基金supported in part by National Institutes of Health T32 AR067708,RO1CA201035the MRB Molecular Imaging Service Center(P50 CA103175)
文摘Discriminating sterile inflammation from infection, especially in cases of aseptic loosening versus an actual prosthetic joint infection, is challenging and has significant treatment implications. Our goal was to evaluate a novel human monoclonal antibody（mAb） probe directed against the Gram-positive bacterial surface molecule lipoteichoic acid（LTA）. Specificity and affinity were assessed in vitro. We then radiolabeled the anti-LTA mAb and evaluated its effectiveness as a diagnostic imaging tool for detecting infection via immuno PET imaging in an in vivo mouse model of prosthetic joint infection（PJI）. In vitro and ex vivo binding of the anti-LTA mAb to pathogenic bacteria was measured with Octet, ELISA, and flow cytometry. The in vivo PJI mouse model was assessed using traditional imaging modalities, including positron emission tomography（PET） with [^（18）F]FDG and [^（18）F]Na F as well as X-ray computed tomography（CT）, before being evaluated with the zirconium-89-labeled antibody specific for LTA（[^（89）Zr]SAC55）.The anti-LTA mAb exhibited specific binding in vitro to LTA-expressing bacteria. Results from imaging showed that our model could reliably simulate infection at the surgical site by bioluminescent imaging, conventional PET tracer imaging, and bone morphological changes by CT. One day following injection of both the radiolabeled anti-LTA and isotype control antibodies, the anti-LTA antibody demonstrated significantly greater（P 〈 0.05） uptake at S. aureus-infected prosthesis sites over either the same antibody at sterile prosthesis sites or of control non-specific antibody at infected prosthesis sites. Taken together, the radiolabeled anti-LTA mAb, [^（89）Zr]SAC55, may serve as a valuable diagnostic molecular imaging probe to help distinguish between sterile inflammation and infection in the setting of PJI. Future studies are needed to determine whether these findings will translate to human PJI.
基金supported by the National Natural Science Foundation of China(21727817,21390414,21425522,51571185)Beijing Science and Technology Commission Special Project for Frontier Technology in Life Sciences(Z171100000417008)
文摘Increasingly serious microbial infections call for the development of new simpler methods for the precise diagnosis and specific inhibition of such pathogens. In this work, a peptide mineralized Au cluster probe was applied as a new simplified strategy to both recognize and inhibit a single bacteria species of Staphylococcus aureus(S. aureus) simultaneously. The probes are composed of peptides and Au clusters. Moreover, the peptides specifically target S. aureus cells and the Au clusters provide fluorescent imaging and have an antibacterial effect. These new probes enable the simultaneous specific detection and effective destruction S. aureus cells in situ.
文摘A functionalized silicon nanowire field-effect transistor （SiNW FET） was fabricated to detect single molecules in the pM range to detect disease at the early stage with a sensitive, robust, and inexpensive method with the ability to provide specific and reliable data. The device was designed and fabricated by indented ash trimming via shallow anisotropic etching. The approach is a simple and low-cost technique that is compatible with the current commercial semiconductor standard CMOS process without an expensive deep reactive ion etcher. Specific electric changes were observed for DNA sensing when the nanowire surface was modified with a complementary captured DNA probe and target DNA through an organic linker （--OCH2CH3） using organofunctional alkoxysilanes （3-aminopropyl） triethoxysilane （APTES）. With this surface modification, a single specific target molecule can be detected. The simplicity of the sensing domain makes it feasible to miniaturize it for the development of a cancer detection kit, facilitating its use in both clinical and non-clinical environments to allow non-expert interpretation. With its novel electric response and potential for mass commercial fabrication, this biosensor can be developed to become a portable/point of care biosensor for both field and diagnostic applications.
文摘Objective To determine the diagnostic significance of detecting the specific epithelial keratin CK-20 mRNA in peripheral venous blood from patients with bladder carcinomas. Methods Reverse transcription coupled with two-step polymerase chain reaction (nested RT-PCR) was used to detect CK-20 mRNA expression in the peripheral blood from patients with blodder carcinomas. Results Detection of CK-20 mRNA expression was positive in 37 of 91 patients with bladder carcinoma (41 % ). Among 20 patients with distant metastasis, 17 were positive (85 % ). CK-20 mRNA was not detectable in the blood samples from 25 normal individuals. The frequency of positive CK-20 mRNA expression was signficantly higher in those with distant metastasis. Conclusion The presence of CK-20 mRNA expression in peripheral blood may be used as an early indicator of hematogenous metastasis of bladder carcinoma cells. 6 refs,1 tab.
基金funded by the National Research Foundation(NRF)of Korea(2020M3H4A3081816,RS-2023-00304936,and RS-2024-00398065).
文摘Various novel conjugated polymers(CPs)have been developed for organic photodetectors(OPDs),but their application to practical image sensors such as X-ray,R/G/B,and fingerprint sensors is rare.In this article,we report the entire process from the synthesis and molecular engineering of novel CPs to the development of OPDs and fingerprint image sensors.We synthesized six benzo[1,2-d:4,5-d’]bis(oxazole)(BBO)-based CPs by modifying the alkyl side chains of the CPs.Several relationships between the molecular structure and the OPD performance were revealed,and increasing the number of linear octyl side chains on the conjugated backbone was the best way to improve Jph and reduce Jd in the OPDs.The optimized CP demonstrated promising OPD performance with a responsivity(R)of 0.22 A/W,specific detectivity(D^(*))of 1.05×10^(13)Jones at a bias of-1 V,rising/falling response time of 2.9/6.9μs,and cut-off frequency(f_(-3dB))of 134 kHz under collimated 530 nm LED irradiation.Finally,a fingerprint image sensor was fabricated by stacking the POTB1-based OPD layer on the organic thin-film transistors(318 ppi).The image contrast caused by the valleys and ridges in the fingerprints was obtained as a digital signal.
基金supported by the Singapore Agency for Science,Technology and Research(A*STAR)(M22K2c0080,R23I0IR041 and M23M2b0056)National Research Foundation Singapore(Award No.NRFCRP22-2019-0007,NRF-CRP29-2022-0003,and NRF-MSG-2023-0002).
文摘The rapid development of modern infrared optoelectronic technology has driven a growing demand for high-sensitivity mid-wave infrared(MWIR)photodetectors capable of reliable room-temperature operation.Achieving optimal specific detectivity,a critical performance metric for MWIR photodetection,remains challenging due to inherent limitations imposed such as high dark current,low optical absorption,or both.To address these challenges,we present an approach based on a bipolar-barrier architecture featuring a black phosphorus(BP)/MoTe_(2)/BP tunnel heterostructure integrated with an Au reflector.This configuration delivers simultaneous electrical and optical enhancement,effectively suppressing dark currents and significantly increasing optical absorption.The bipolar-barrier structure minimizes dark current by blocking thermally excited and bias-induced carrier leakage,while facilitating efficient tunneling of photogenerated carriers via trap-assisted photogating mechanisms.In addition,the Au reflector enhances optical absorption through interference effects.As a result,the heterostructure achieves remarkable performance metrics,including a room-temperature specific detectivity of~3.0×10^(10)cm Hz0.5 W^(-1),a high responsivity of~4 A W^(-1),and an external quantum efficiency of~140%within the MWIR range.These results establish the bipolar-barrier tunnel heterostructure as a highly efficient platform,paving the way for the next generation of advanced infrared optoelectronic devices.
基金supported in part by the National Key Research and Development Program of China(2022YFA1404004)the National Natural Science Foundation of China(62435010,62335012,61988102).
文摘Terahertz metamaterial biosensors combine terahertz time-domain spectroscopy with metamaterial sensing to provide a sensitive detection platform for a variety of targets,including biological molecules,proteins,cells,and viruses.These biosensors are characterized by their rapid response,sensitivity,non-destructive,label-free operation,minimal sample requirement,and user-friendly design,which also allows for integration with various technical approaches.Advancing beyond traditional biosensors,terahertz metamaterial biosensors facilitate rapid and non-destructive trace detection in biomedical applications,contributing to timely diagnosis and early screening of diseases.In this paper,the theoretical basis and advanced progress of these biosensors are discussed in depth,focusing on three key areas:improving the sensitivity and specificity,and reducing the influence of water absorption in biological samples.This paper also analyzes the potential and future development of these biosensors for expanded applications.It highlights their potential for multi-band tuning,intelligent operations,and flexible,wearable biosensor applications.This review provides a valuable reference for the follow-up research and application of terahertz metamaterial biosensors in the field of biomedical detection.
基金the National Natural Science Foundation of China(Grants 12204272,52073162,52272157)Taishan Scholars Program(Grant tstp20230610)+2 种基金Shandong Provincial Natural Science Foundation(Grants ZR2022YQ04,ZR2021QF016)State Key Laboratory of Crystal Materials(Grant KF2209)the Hubei Longzhong Laboratory(Grant 2022KF-01)for financial support.
文摘Broad-spectrum photodetectors(PDs)are essential for various health monitoring,night vision,and telecommunications applications,but their detectivity in a wide absorbance region is limited by undesirable electronic response properties.Colloidal quantum dots(CQDs)are a promising system for broad-spectrum detection,whereas their practical potential is hindered by suboptimal dark current characteristics.To overcome these challenges,we propose a layered architecture comprising CQDs and a bulk heterojunction(BHJ)organic film as a hole transport layer.The integration of PbS CQDs offers multiple benefits,including bandgap tuning for minimizing thermal carriers,surface passivation to reduce recombination rates,and the formation of high-quality interfaces with organic layers,which collectively contribute to suppressing dark current leakage and thermal excitations by suppressing stray electrons.By integrating ITIC into the BHJ film,the device detectability is significantly enhanced,reaching 1013 Jones in the 400–1000 nm spectral range.This improvement is attributed to the higher lowest unoccupied molecular orbital(LUMO)of ITIC molecules,which effectively hinders electron injection.Additionally,J-aggregation-induced molecular stacking and optimized phase separation of BHJ films contribute to the enhanced performance.The integration of diverse materials offers greater flexibility in device design and functionality,enabling the development of more advanced and sophisticated optoelectronic devices.Furthermore,this approach could significantly enhance the theoretical and practical understanding of optoelectronic device engineering,leading to the development of more advanced optoelectronic devices.
文摘Subject Code:H30With the support by the National Natural Science Foundation of China and National Basic Research Program of China,the group led by Prof.Ge Guangbo(葛广波)and Prof.Yang Ling(杨凌)from the Laboratory of Pharmaceutical Resource Discovery,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,reported a highly specific ratiometric two-photon fluorescent probe to detect
基金the International Cooperation Foundation of China (2015DFR10700)the National Natural Science Foundation of China (51403203) for the support of this researchthe support of the Russian Ministry of Education and Science state assignment (3.3197.2017/ПЧ)
文摘Organic-inorganic hybrid perovskites are ideal materials for photodetection owing to their high charge carrier mobility, long charge carrier diffusion length, low dark current density and sharp absorption edge. However, a relatively small band gap(1.6 e V) limits their photonharvesting efficiency in the near-infrared region. In the present work, we demonstrate a hybrid methylamine iodide and Pb-Sn binary perovskite as the light absorption layer in photodetectors. Experimentally, the wavelength of photoresponse onset for the photodetectors can be extended to as great as 1,000 nm when the Sn content of the hybrid perovskite is increased to 30 mol%. In addition, the photodetectors exhibit a photoresponsivity of 0.39 A W^-1, a specific detectivity of 7×10^12 Jones, a fast photoresponse with rise and decay time constants and an external quantum efficiency greater than 50% in the wavelength range of350–900 nm, with a maximum value of about 80% at 550 nm.
