To evaluate the quality of Polyporus umbellatus,we established a simple,repeatable and reliable method based on high performance liquid chromatography(HPLC)for specific chromatogram and fingerprint analysis,which was ...To evaluate the quality of Polyporus umbellatus,we established a simple,repeatable and reliable method based on high performance liquid chromatography(HPLC)for specific chromatogram and fingerprint analysis,which was applied to analyze samples of medicinal materials and decoction pieces collected from different regions.Finally,ten characteristic peaks were designated in the specific chromatograms and applied to the authenticate identification of P.umbellatus samples.Nine common peaks were designated in the fingerprints,and then the similarities between 32 batches of samples were calculated.Among them,eight compounds were identified by HPLC-APCI-IT-TOF-MS^(n),four of which were identified in specific chromatograms and four in fingerprints.In the present study,we,for the first time,combined HPLC specific chromatograms and fingerprints for the species identification and quality evaluation of P.umbellatus.Collectively,our findings provided a new method for establishing a comprehensive quality standard of P.umbellatus.展开更多
DNA barcoding and HPLC specific chromatogram were used to identify three kinds of Plumeria flowers respectively. DNAs extracted from the three Plumeria species were amplified by PCR with universal primers, and the psb...DNA barcoding and HPLC specific chromatogram were used to identify three kinds of Plumeria flowers respectively. DNAs extracted from the three Plumeria species were amplified by PCR with universal primers, and the psbA-trnH region was selected. All the amplified products were sequenced and the results were analyzed by MEGA 5.0. Chemometric methods including principal components analysis and hierarchical clustering analysis were conducted on the SAS 9.0 software to demonstrate the variability among samples. In conclusion, the psbA-trnH of all samples were successfully amplified from total DNA and sequenced. These three varieties of Plumeria can be differentiated by the psbA-trnH region and clustered into three groups respectively through building neighbor joining tree, which conformed to their germplasm origins. However, it was hard to distinguish them by HPLC specific chromatograms combined with chemometrics analysis. These indicated that DNA barcoding was a promising and reliable tool for the identification of three kinds of Plumeria flowers compared to HPLC specific chromatogram generally used. It could be treated as a powerful complementary method for traditional authentication, especially for those varieties which are difficult to be identified by conventional chromatography.展开更多
Ganoderma polysaccharides(peptides)are essential bio-macromoleculars that determine the quality of Ganoderma.Their molecular weight,distribution and content are influenced by factors such as origin,strain,cultivation ...Ganoderma polysaccharides(peptides)are essential bio-macromoleculars that determine the quality of Ganoderma.Their molecular weight,distribution and content are influenced by factors such as origin,strain,cultivation conditions,harvesting period and processing methods.In this study,we established a molecular weight specific chromatogram using high performance gel permeation chromatography(HPGPC)for Ganoderma glycopeptide and assessed its potential applications.The results revealed consistent molecular weight distributions across different cultivation conditions,with 6–8 characteristic peaks showing similarity values exceeding 0.93.The average molecular weight of common peaks was highest during the budding stage and lowest in the mature stage.Ganoderma lucidum(GL)exhibited higher molecular weights compared to Ganoderma sinense(GS).Moreover,both molecular weight and Ganoderma glycopeptides decreased with prolonged growth periods.The content of GL-PPSQ2 was significantly higher in the budding(1.63±0.15%)and cap-opening stages(1.51±0.12%)compared to the mature stage(1.31±0.05%).Chemometrics analysis revealed distinct molecular weight profiles for different growth stages and varieties.Additionally,the HPGPC method was successfully applied to detect adulteration in Ganoderma extracts,identifying excipients such as maltodextrin and dextran.This study underscores the importance of molecular weight distribution and glycopeptide content as key quality control indicators,providing a rapid and reliable tool for ensuring the authenticity and quality of Ganoderma extracts in the market.展开更多
基金National Project for Standardization of Chinese Material Medica(Grant No.ZYBZH-Y-GD-13)。
文摘To evaluate the quality of Polyporus umbellatus,we established a simple,repeatable and reliable method based on high performance liquid chromatography(HPLC)for specific chromatogram and fingerprint analysis,which was applied to analyze samples of medicinal materials and decoction pieces collected from different regions.Finally,ten characteristic peaks were designated in the specific chromatograms and applied to the authenticate identification of P.umbellatus samples.Nine common peaks were designated in the fingerprints,and then the similarities between 32 batches of samples were calculated.Among them,eight compounds were identified by HPLC-APCI-IT-TOF-MS^(n),four of which were identified in specific chromatograms and four in fingerprints.In the present study,we,for the first time,combined HPLC specific chromatograms and fingerprints for the species identification and quality evaluation of P.umbellatus.Collectively,our findings provided a new method for establishing a comprehensive quality standard of P.umbellatus.
基金supported by the Industry-University-Research Cooperation Program from Science and Technology Department of Guangdong Province (No.: 2013B090600058)the National Key Research and Development Program of China (2017YFC170116)
文摘DNA barcoding and HPLC specific chromatogram were used to identify three kinds of Plumeria flowers respectively. DNAs extracted from the three Plumeria species were amplified by PCR with universal primers, and the psbA-trnH region was selected. All the amplified products were sequenced and the results were analyzed by MEGA 5.0. Chemometric methods including principal components analysis and hierarchical clustering analysis were conducted on the SAS 9.0 software to demonstrate the variability among samples. In conclusion, the psbA-trnH of all samples were successfully amplified from total DNA and sequenced. These three varieties of Plumeria can be differentiated by the psbA-trnH region and clustered into three groups respectively through building neighbor joining tree, which conformed to their germplasm origins. However, it was hard to distinguish them by HPLC specific chromatograms combined with chemometrics analysis. These indicated that DNA barcoding was a promising and reliable tool for the identification of three kinds of Plumeria flowers compared to HPLC specific chromatogram generally used. It could be treated as a powerful complementary method for traditional authentication, especially for those varieties which are difficult to be identified by conventional chromatography.
基金supported by the Special Project for the Protection and Utilization of Agricultural Resources of the Department of Agri-culture and Rural Affairs of Fujian Province“Research and Application of Key Technologies for Innovation and Industrialized Utilization on Juncao and Juncao Mushrooms”(22001XA)the Major Special Project of Fujian Province“Research and application of key technologies for innovation and industrialized utilization of Juncao”(2021NZ029009)Fujian Agriculture and Forestry University“Interdisciplinary integration to promote the high-quality development of Juncao science and Industry”(XKJC-712021030).
文摘Ganoderma polysaccharides(peptides)are essential bio-macromoleculars that determine the quality of Ganoderma.Their molecular weight,distribution and content are influenced by factors such as origin,strain,cultivation conditions,harvesting period and processing methods.In this study,we established a molecular weight specific chromatogram using high performance gel permeation chromatography(HPGPC)for Ganoderma glycopeptide and assessed its potential applications.The results revealed consistent molecular weight distributions across different cultivation conditions,with 6–8 characteristic peaks showing similarity values exceeding 0.93.The average molecular weight of common peaks was highest during the budding stage and lowest in the mature stage.Ganoderma lucidum(GL)exhibited higher molecular weights compared to Ganoderma sinense(GS).Moreover,both molecular weight and Ganoderma glycopeptides decreased with prolonged growth periods.The content of GL-PPSQ2 was significantly higher in the budding(1.63±0.15%)and cap-opening stages(1.51±0.12%)compared to the mature stage(1.31±0.05%).Chemometrics analysis revealed distinct molecular weight profiles for different growth stages and varieties.Additionally,the HPGPC method was successfully applied to detect adulteration in Ganoderma extracts,identifying excipients such as maltodextrin and dextran.This study underscores the importance of molecular weight distribution and glycopeptide content as key quality control indicators,providing a rapid and reliable tool for ensuring the authenticity and quality of Ganoderma extracts in the market.
