Adult rice field eels with 125.52±27.99 g body weight corresponding to 44.79±5.93 cm in length were maturity cultured in a 5 m^2 cement tank without mud for three months with density 30 eels/m^2.Eel broodsto...Adult rice field eels with 125.52±27.99 g body weight corresponding to 44.79±5.93 cm in length were maturity cultured in a 5 m^2 cement tank without mud for three months with density 30 eels/m^2.Eel broodstocks were fed with three types of food including:sea fish(T1),commercial pellet food(T2)and mixed food—50%sea fish and 50%commercial pellet food(T3).Result showed that the maximum gonadosomatic index(GSI)of female eels fed by sea fish(2.89%±0.67%)was higher than both commercial pellet food(1.62%±0.62%)and mixed food(2.03%±0.82%)(P<0.05),while,GSI of male eels was 1.27%±0.31%,0.68%±0.23%and 1.14%±0.41%(P>0.05).Maturity rate of female fed by sea fish(88.91%±9.64%)was higher than commercial pellet food(61.12%±9.64%)(P<0.05).The maturity rate of male eels was rather low and there was not significantly different among treatments(P>0.05).Then.the eels were induced for spawning with two kinds of hormones,including T1:human chorionic ganadotropin(HCG)at 1,500 IU/kg,T2:luteinzing hormone-releasing hormone(LHRH-a)at 150μg/kg and domperidon 10 mg/kg,and T3:control without hormone.The results of spawning induction methods showed that the reproduction rate of female eels induced by LHRH-a and HCG hormones were higher than that by natural reproduction method(P<0.05).Others,the survival rate of fry eels after 5 d using natural reproduction method(92.65%±2.54%)was higher than both using LHRH-a(67.77%±1.91%)and HCG(68.65%±1.23%)hormones(P<0.05).The average diameter of eggs was 3.40-3.41 mm and the length of newly hatched eels was 1.72±0.19 cm.展开更多
文摘Adult rice field eels with 125.52±27.99 g body weight corresponding to 44.79±5.93 cm in length were maturity cultured in a 5 m^2 cement tank without mud for three months with density 30 eels/m^2.Eel broodstocks were fed with three types of food including:sea fish(T1),commercial pellet food(T2)and mixed food—50%sea fish and 50%commercial pellet food(T3).Result showed that the maximum gonadosomatic index(GSI)of female eels fed by sea fish(2.89%±0.67%)was higher than both commercial pellet food(1.62%±0.62%)and mixed food(2.03%±0.82%)(P<0.05),while,GSI of male eels was 1.27%±0.31%,0.68%±0.23%and 1.14%±0.41%(P>0.05).Maturity rate of female fed by sea fish(88.91%±9.64%)was higher than commercial pellet food(61.12%±9.64%)(P<0.05).The maturity rate of male eels was rather low and there was not significantly different among treatments(P>0.05).Then.the eels were induced for spawning with two kinds of hormones,including T1:human chorionic ganadotropin(HCG)at 1,500 IU/kg,T2:luteinzing hormone-releasing hormone(LHRH-a)at 150μg/kg and domperidon 10 mg/kg,and T3:control without hormone.The results of spawning induction methods showed that the reproduction rate of female eels induced by LHRH-a and HCG hormones were higher than that by natural reproduction method(P<0.05).Others,the survival rate of fry eels after 5 d using natural reproduction method(92.65%±2.54%)was higher than both using LHRH-a(67.77%±1.91%)and HCG(68.65%±1.23%)hormones(P<0.05).The average diameter of eggs was 3.40-3.41 mm and the length of newly hatched eels was 1.72±0.19 cm.