[Objective] The aim was to study the optimization of bio-emulsifier production by Geobacillus sp.XS2 based on response surface methodology.[Method] Firstly,single factor experiment was conducted to find out the main m...[Objective] The aim was to study the optimization of bio-emulsifier production by Geobacillus sp.XS2 based on response surface methodology.[Method] Firstly,single factor experiment was conducted to find out the main medium components influencing bio-emulsifier production by Geobacillus sp.XS2,and then response surface model was established by using response surface methodology and Design-Expert 7.0,so as to optimize the fermentation medium for bio-emulsifier production by Geobacillus sp.XS2.[Result] Glucose,KH2PO4 and K2HPO4 were the main impact factors of bio-emulsifier production by Geobacillus sp.XS2,and its optimal fermentation medium was composed of 68 g/L glucose,2 g/L NaNO3,5.03 g/L KH2PO4,1.36 g/L K2HPO4,0.2 g/L MgSO4·7H2O,0.02 g/L FeSO4·7H2O,0.01 g/L CaCl2·2H2O and 2 ml microelement solution.Under the optimal conditions,the measured emulsifying activity of bio-emulsifier (67.0%) was close to predictive value (66.7%) and increased by 27% compared with previous value before optimization.[Conclusion] Response surface methodology was applicable to the optimization of fermentation medium of bio-emulsifier production by Geobacillus sp.XS2,and its optimal result was consistent with actual result.展开更多
基金Supported by Knowledge Innovation Project Group of Chinese Academy of Sciences (KZCX2-YW-Q05-05)
文摘[Objective] The aim was to study the optimization of bio-emulsifier production by Geobacillus sp.XS2 based on response surface methodology.[Method] Firstly,single factor experiment was conducted to find out the main medium components influencing bio-emulsifier production by Geobacillus sp.XS2,and then response surface model was established by using response surface methodology and Design-Expert 7.0,so as to optimize the fermentation medium for bio-emulsifier production by Geobacillus sp.XS2.[Result] Glucose,KH2PO4 and K2HPO4 were the main impact factors of bio-emulsifier production by Geobacillus sp.XS2,and its optimal fermentation medium was composed of 68 g/L glucose,2 g/L NaNO3,5.03 g/L KH2PO4,1.36 g/L K2HPO4,0.2 g/L MgSO4·7H2O,0.02 g/L FeSO4·7H2O,0.01 g/L CaCl2·2H2O and 2 ml microelement solution.Under the optimal conditions,the measured emulsifying activity of bio-emulsifier (67.0%) was close to predictive value (66.7%) and increased by 27% compared with previous value before optimization.[Conclusion] Response surface methodology was applicable to the optimization of fermentation medium of bio-emulsifier production by Geobacillus sp.XS2,and its optimal result was consistent with actual result.
