study was conducted on the biologically active secondary metabolites produced by the marine fungus Cladosporium sp.MDW-211.Preparative high performance liquid chromatography(HPLC)and other techniques were used to isol...study was conducted on the biologically active secondary metabolites produced by the marine fungus Cladosporium sp.MDW-211.Preparative high performance liquid chromatography(HPLC)and other techniques were used to isolate and purify the compounds,the structures of the compounds 1 were determined by high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),nuclear magnetic resonance(NMR)spectroscopy,X-ray and other methods,and the pro-angiogenic activity of the compounds was evaluated by zebrafish model.One new dihydroisocoumarin 1(4'S-hydroxyasperentin)and three known dihydroisocoumarins 2~4 were obtained and identified.Bioactivity assays revealed that compound 3 exhibited pro-angiogenic activity at concentrations of 20 and 40μmol/L,which is reported here for the first time.展开更多
Methomyl, an extremely toxic pesticide, is widely used in agriculture. A strain named mdw-1 capable of degrading methomyl rapidly was successfully isolated from activated sludge in this study. It could utilize methomy...Methomyl, an extremely toxic pesticide, is widely used in agriculture. A strain named mdw-1 capable of degrading methomyl rapidly was successfully isolated from activated sludge in this study. It could utilize methomyl as the sole carbon or nitrogen source. The optimal temperature and medium pH for its growth and methomyl biodegradation were 30℃ and 7.0, respectively. It was identified as a Paracoccus sp. according to its morphological features, physiological and biochemical characteristics, and phylogenetic analysis based on the sequence of 16S rDNA. Gas chromatography-mass spectrometry （GC-MS） analysis showed that methomyl could be completely transformed to S-methyl-N-hydroxythioacetamidate in 10 h of incubation with the isolate mdw-1.展开更多
基金Project supported by the National Natural Science Foundation of China(No.82204276)the Guangxi Natural Science Foundation(No.2025GXNSF-AA069557)+1 种基金the Guangxi Scholarship Fund of Guangxi Education Departmentthe Innovation Project of Guangxi Minzu University Graduate Education(No.gxmzu-chxs2024225)。
文摘study was conducted on the biologically active secondary metabolites produced by the marine fungus Cladosporium sp.MDW-211.Preparative high performance liquid chromatography(HPLC)and other techniques were used to isolate and purify the compounds,the structures of the compounds 1 were determined by high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),nuclear magnetic resonance(NMR)spectroscopy,X-ray and other methods,and the pro-angiogenic activity of the compounds was evaluated by zebrafish model.One new dihydroisocoumarin 1(4'S-hydroxyasperentin)and three known dihydroisocoumarins 2~4 were obtained and identified.Bioactivity assays revealed that compound 3 exhibited pro-angiogenic activity at concentrations of 20 and 40μmol/L,which is reported here for the first time.
基金Project supported by the National Natural Science Foundation of China(No.30600016)the National High Technology Research and Development Program of China(863 Program)(No.2007AA10Z405)+2 种基金the National Program for Sci-Tech Basic Platform Construction of China(No.2005DKA21201-2)the Program for Science and Technology Development ofJiangsu Province,China(No.BE2008669)the Program for Integration of Production,Education,and Research of Guangdong Province and Ministry of Education,China(No.2006090204007)
文摘Methomyl, an extremely toxic pesticide, is widely used in agriculture. A strain named mdw-1 capable of degrading methomyl rapidly was successfully isolated from activated sludge in this study. It could utilize methomyl as the sole carbon or nitrogen source. The optimal temperature and medium pH for its growth and methomyl biodegradation were 30℃ and 7.0, respectively. It was identified as a Paracoccus sp. according to its morphological features, physiological and biochemical characteristics, and phylogenetic analysis based on the sequence of 16S rDNA. Gas chromatography-mass spectrometry （GC-MS） analysis showed that methomyl could be completely transformed to S-methyl-N-hydroxythioacetamidate in 10 h of incubation with the isolate mdw-1.
