Continuous-cropping(CC)obstacles are among the primary factors limiting the development of global agri-culture.Although most plants are negatively affected by barriers that develop with CC,they may also over-come such...Continuous-cropping(CC)obstacles are among the primary factors limiting the development of global agri-culture.Although most plants are negatively affected by barriers that develop with CC,they may also over-come such obstacles by altering the soil biological and chemical environment to favor plant growth.In this study,we investigated the mechanism by which plants recruit beneficial microorganisms using root exu-dates to alleviate obstacles in a 10-year CC potato system.On day 20 after potato emergence,soil micro-organisms in the CC system promoted an increase in adventitious root(AR)numbers by increasing the indole-3-acetic acid(IAA)content of the rhizosphere soil.Analysis of rhizosphere bacterial communities using 16S rRNA sequencing revealed that CC alters community structure,increasing the abundance of Pantoea sp.MCC16.Irrigation with root exudates from CC potato significantly increased AR numbers and Pantoea sp.MCC16 abundance.Through untargeted metabolomic analysis,we identified nobiletin as key metabolite that promotes Pantoea sp.MCC16 colonization in the rhizosphere.Furthermore,appli-cation of either nobiletin or Pantoea sp.MCC16 significantly improved the yield of CC potatoes.These find-ings demonstrate that CC plants can actively secrete the unique metabolite nobiletin to recruit Pantoea sp.MCC16,a high IAA producer,to help plants recover functional traits and mitigate CC obstacles.展开更多
基金supported by the Natural Science Foundation of Si-chuan Province(grant number 2022NSFSC0014)the Tackling Key Prob-lems and Supporting Projects of Breeding in Sichuan Province(grant numbers 2021YFYZ0019 and 2021YFYZ0005)+1 种基金the Sichuan Potato Inno-vation Team(grant number sccxtd-2023-09)the National Natural Science Foundation of China(grant number 32060443).
文摘Continuous-cropping(CC)obstacles are among the primary factors limiting the development of global agri-culture.Although most plants are negatively affected by barriers that develop with CC,they may also over-come such obstacles by altering the soil biological and chemical environment to favor plant growth.In this study,we investigated the mechanism by which plants recruit beneficial microorganisms using root exu-dates to alleviate obstacles in a 10-year CC potato system.On day 20 after potato emergence,soil micro-organisms in the CC system promoted an increase in adventitious root(AR)numbers by increasing the indole-3-acetic acid(IAA)content of the rhizosphere soil.Analysis of rhizosphere bacterial communities using 16S rRNA sequencing revealed that CC alters community structure,increasing the abundance of Pantoea sp.MCC16.Irrigation with root exudates from CC potato significantly increased AR numbers and Pantoea sp.MCC16 abundance.Through untargeted metabolomic analysis,we identified nobiletin as key metabolite that promotes Pantoea sp.MCC16 colonization in the rhizosphere.Furthermore,appli-cation of either nobiletin or Pantoea sp.MCC16 significantly improved the yield of CC potatoes.These find-ings demonstrate that CC plants can actively secrete the unique metabolite nobiletin to recruit Pantoea sp.MCC16,a high IAA producer,to help plants recover functional traits and mitigate CC obstacles.
