As a major component of lignin and abundantly existing in softwood and hardwood,ferulic acid has been used as a lignin-related compound for lignin biodegradation study.Biodegradation of ferulic acid by Cupriavidus sp....As a major component of lignin and abundantly existing in softwood and hardwood,ferulic acid has been used as a lignin-related compound for lignin biodegradation study.Biodegradation of ferulic acid by Cupriavidus sp.B-8,a newly isolated strain,was studied.This strain is able to utilize a wide range of lignin-related aromatic compounds as the sole carbon and energy source,including guaiacol,veratric acid,vanillic acid,cinnamic acid,p-coumaric acid,ferulic acid,and sinapic acid.In addition,the effects of different concentrations of ferulic acid on growth of Cupriavidus sp.B-8 were studied.The growth of Cupriavidus sp.B-8 is better under the condition of lower concentration.High-performance liquid chromatography(HPLC)analysis reveals that above 95%of ferulic acid is degraded within 12 h by Cupriavidus sp.B-8.Based on identification of biodegradation intermediates and further metabolites,the biodegradation pathway of ferulic acid by Cupriavidus sp.B-8 was proposed.Ferulic acid is initially converted to 4-vinylguaiacol,and further oxidized to vanillic acid and protocatechuic acid.展开更多
基金Project(50925417)supported by the National Science Fund for Distinguished Young Scholars of ChinaProject(50830301)supported by the National Natural Science Foundation of ChinaProject(2011467062)supported by National Research Fund for Public Benefit(Environmental Protection)Industries,China
文摘As a major component of lignin and abundantly existing in softwood and hardwood,ferulic acid has been used as a lignin-related compound for lignin biodegradation study.Biodegradation of ferulic acid by Cupriavidus sp.B-8,a newly isolated strain,was studied.This strain is able to utilize a wide range of lignin-related aromatic compounds as the sole carbon and energy source,including guaiacol,veratric acid,vanillic acid,cinnamic acid,p-coumaric acid,ferulic acid,and sinapic acid.In addition,the effects of different concentrations of ferulic acid on growth of Cupriavidus sp.B-8 were studied.The growth of Cupriavidus sp.B-8 is better under the condition of lower concentration.High-performance liquid chromatography(HPLC)analysis reveals that above 95%of ferulic acid is degraded within 12 h by Cupriavidus sp.B-8.Based on identification of biodegradation intermediates and further metabolites,the biodegradation pathway of ferulic acid by Cupriavidus sp.B-8 was proposed.Ferulic acid is initially converted to 4-vinylguaiacol,and further oxidized to vanillic acid and protocatechuic acid.
