根据荷斯坦牛SRY基因设计一对引物,采用聚合酶链式反应(PCR)技术,以中国沼泽型水牛(Swamp Buffalo)基因组DNA为模板,扩增得到SRY(Sex deterimation region of Y chromosome)全序列约2005bp,其中1-504bp为5’启动子区,1196-2005bp为3’...根据荷斯坦牛SRY基因设计一对引物,采用聚合酶链式反应(PCR)技术,以中国沼泽型水牛(Swamp Buffalo)基因组DNA为模板,扩增得到SRY(Sex deterimation region of Y chromosome)全序列约2005bp,其中1-504bp为5’启动子区,1196-2005bp为3’侧翼序列,在505-1195bp为SRY的外显子,编码229个氨基酸。在SRY HMG-box区域设计探针,用地高辛标记后分别与雄性、雌性水牛基因组DNA进行Southern杂交,结果显示该段序列只在雄性DNA样本中有杂交信号,证明SRY基因为雄性特异。BLAST比对结果显示与牛属动物SRY基因的同源性为96％,其中SRY基因HMG box区域同源性高达99％,说明SRY基因具有高度的进化保守性。展开更多
The pathogen of southern blight, distribution and host species, damage symptoms and infection cycle, and the pathogenic mechanisms are introduced. Based on the introduction, the major factors affected the incidence of...The pathogen of southern blight, distribution and host species, damage symptoms and infection cycle, and the pathogenic mechanisms are introduced. Based on the introduction, the major factors affected the incidence of medicinal plants such as climatic conditions, soil conditions and cultivation conditions, etc. are further summarized, and the studies on the control of southern blight in Chinese medicinal plants are summarized from the aspects of agricultural control, biological control and chemical control, etc.展开更多
文摘根据荷斯坦牛SRY基因设计一对引物,采用聚合酶链式反应(PCR)技术,以中国沼泽型水牛(Swamp Buffalo)基因组DNA为模板,扩增得到SRY(Sex deterimation region of Y chromosome)全序列约2005bp,其中1-504bp为5’启动子区,1196-2005bp为3’侧翼序列,在505-1195bp为SRY的外显子,编码229个氨基酸。在SRY HMG-box区域设计探针,用地高辛标记后分别与雄性、雌性水牛基因组DNA进行Southern杂交,结果显示该段序列只在雄性DNA样本中有杂交信号,证明SRY基因为雄性特异。BLAST比对结果显示与牛属动物SRY基因的同源性为96％,其中SRY基因HMG box区域同源性高达99％,说明SRY基因具有高度的进化保守性。
文摘The pathogen of southern blight, distribution and host species, damage symptoms and infection cycle, and the pathogenic mechanisms are introduced. Based on the introduction, the major factors affected the incidence of medicinal plants such as climatic conditions, soil conditions and cultivation conditions, etc. are further summarized, and the studies on the control of southern blight in Chinese medicinal plants are summarized from the aspects of agricultural control, biological control and chemical control, etc.
