Acupoint combination is a method used for acupoint treatment of patients. Traditionally, acupoints are matched along the meridian distribution, which is a common rule in clinical practice, but the underlying mechanism...Acupoint combination is a method used for acupoint treatment of patients. Traditionally, acupoints are matched along the meridian distribution, which is a common rule in clinical practice, but the underlying mechanism remains unclear. Cerebral scans with functional magnetic resonance imaging （fMRI） have been used in the study of acupuncture and acupoint specifically. In this study, fMRI was used to detect the activation of the brain areas under different acupoints, matched along different meridians, to elucidate the acupoint combination via a modern medical approach. Forty healthy volunteers were randomly divided into the following groups： Waiguan point （SJ 5）, Waiguan （SJ 5） ＋ Zhigou （SJ 6） （2 acupoints come from the same meridian）, Waiguan （SJ 5） ＋ Neiguan （PC 6） （2 acupoints come from 2 meridians with the relationship of interior-exterior）, Waiguan （SJ 5） ＋ Yanglingquan （GB 34） （2 acupoints come from 2 meridians with the same name-Shaoyang Meridian）, and sham point groups （needling in different points on the right hand）. A real-time cerebral fMRI scan was simultaneously performed. The cerebral activation rate, and the number and strength of different regions of interest were compared among the groups. The fMRI cerebral imaging confirmed that there were some differences in the activation of cerebral areas by the needlings in SJ 5, and in combination with other acupoints. Needling at SJ 5 alone greatly activated the right cerebellum, while needling at both SJ 5 and different co-needling points activated different cerebral functional areas.展开更多
Objective To observe the influence of different acupoint combinations on immediate effect of surface electromyography of patients with cervical spondylosis, and to explore the interactions and laws among different acu...Objective To observe the influence of different acupoint combinations on immediate effect of surface electromyography of patients with cervical spondylosis, and to explore the interactions and laws among different acupoint combinations. Methods Acupuncture in three kinds of different acupoint combinations was conducted on 90 patients with cervical spondylosis（three groups）： group A [distal point selection group： Kūnlún（昆仑 BL 60） and Hòuxī（后溪 SI 3）]; group B [local point selection group： Fēngchí（风池 GB 20） and Tiānzhù（天柱 BL 10）]; group C（distal and local point selection group： BL 60, SI 3, GB 20 and BL 10）. Self control before and after treatment was adopted to observe the mean value of surface electromyography（SEMG） amplitude, to measure the mean values of integrated electromyography（IEMG） and electromyography root mean square（RMS）, and to calculate the variation rate of electromyography amplitude of trapezius on the affected side of patients before and after acupuncture. The differences of influences of different acupoint combinations on immediate effect of surface electromyography were compared intra-group, and the differences among the three groups after acupuncture were also compared. Results According to the intragroup comparison before and after acupuncture, the differences of mean value of electromyography amplitude, variation rate of electromyography amplitude, IEMG and RMS were both statistically significant（all P〈0.05）. According to the inter-group comparison after acupuncture, the mean value of electromyography amplitude, variation rate of electromyography amplitude, IEMG and RMS varied in different degrees, however, the differences were not significant（all P〉0.05）; there was no significant difference in the total effective rate among the three groups（P〉0.05）. Conclusion Acupuncture treatment in different acupoint combinations can increase IEMG and RMS and reduce mean value of electromyography amplitude and variation rate of electromyography amplitude, enhance cervical vertebral stability and active contractility of muscle fiber, and improve the fatigue resistance of neck flexion; while, the therapeutic effect of acupuncture on cervical spondylosis has nothing to do with the distance or the number of selected acupoint.展开更多
Objective:To analyze the rule of acupoint combination of insomnia treated with modern acupuncturemoxibustion with complex network technology and further reveal the internal characteristics of acupoint combination.Meth...Objective:To analyze the rule of acupoint combination of insomnia treated with modern acupuncturemoxibustion with complex network technology and further reveal the internal characteristics of acupoint combination.Methods:Using computer retrieval,the relevant clinical trials of acupuncture and moxibustion in treatment of insomnia were searched from China National knowledge Infrastructure(CNKI),Wanfang Data Knowledge Service Platform(Wanfang),VIP Chinese Journal Service Platform(VIP) and Pubmed Database in recent 5 years.The articles were screened according to the inclusion and exclusion criteria,and then the database was set up and the acupoint prescriptions of acupuncture and moxibustion were extracted.Using Excel 2016 software,the data were processed and the frequency analysis was conducted.With SPSS Modeler 18.0,the association rules were analyzed.Using Gephi 0.9.2,the community analysis and complex network analysis were conducted.Results:Finally,766 articles were eligible and 1276 acupoint prescriptions were extracted with 186 acupoints involved.The acupoints with the highest frequency of use were Shénmén(神门 HT7),Bǎihuì(百会GV20) and Sānyīnjiāo(三阴交SP6).The meridians with the highest frequency were bladder meridian and the governor vessel.The acupoints located on the head and foot were mostly selected.The specific points involved were the crossing points,five-shu points and yuan-source points.The main core paired points referred to HT7 and SP6,HT7 and GV20 as well as HT7 and Nèiguān(内关PC6).The meridians with the highest confidence level appeared among heart meridian of hand-shaoyin,spleen meridian of foot-taiyin and kidney meridian of foot-shaoyin.The modular analysis of complex network obtained 4 core communities and topology analysis obtained 35 core points.The main prescription was composed of HT7,GV20,SP6,Sìshéncōng(四神聪EX-HN1),PC6,Tàixī(太溪KI3),Zúsānlǐ(足三里ST36),Tàichōng(太冲LR3),Anmián(安眠EX-HN18),Yìntáng(印堂EX-HN3),Zhàohǎi(照海KI6),Shēnmài(申脉BL62) and Fēngchí(风池 GB20).Conclusion:Acupuncture-moxibustion therapy focuses on regulating the mind/spirit of the heart and brain,as well as of five zang organs.The general treatment principles are regaining consciousness and opening the orifice,replenishing yin and reducing yang,nourishing the heart and tranquilizing.The supplementary points are mostly the combination of distal and nearby points and the combination of the acupoints located in the upper and the lower parts of the body.The back-shu points and the front-mu points are generally used,while the crossing point as the specific point is especially applied.All of these summaries provide the reference and guidance for clinical application and scientific research.展开更多
OBJECTIVE:To investiage the effect of electroacupuncture(EA)at a single acupoint of Shenmen(HT7),Baihui(GV20),Sanyinjiao(SP6)and at combined acupoints of Shenmen(HT7)and Baihui(GV20)and Sanyinjiao(SP6)on the PKA/CREB ...OBJECTIVE:To investiage the effect of electroacupuncture(EA)at a single acupoint of Shenmen(HT7),Baihui(GV20),Sanyinjiao(SP6)and at combined acupoints of Shenmen(HT7)and Baihui(GV20)and Sanyinjiao(SP6)on the PKA/CREB and BDNF/TrkB signaling,as well as neuroapoptosis and neurogenesis in hippocampus and elucidate the underlying mechanism of single and combined acupoints on ameliorating spatial learning and memory deficits in a rat model of primary insomnia.METHODS:Primary insomnia was modeled by intraperitoneal injection of para-chlorophenylalanine(PCPA)once daily for 2 d.EA was applied at Shenmen(HT7),Baihui(GV20),Sanyinjiao(SP6),or Shenmen(HT7)+Baihui(GV20)+Sanyinjiao(SP6)(combined)for 30 min daily for 4 d.Spatial learning and memory function was evaluated by the Morris water maze(MWM)test.Protein expressions of hippocampal cAMP-dependent protein kinase(PKA)-Cβ,phosphorylated cAMP-responsive element-binding protein(p-CREB),brainderived neurotrophic factor(BDNF),and tyrosine kinase receptor B(TrkB)were evaluated by Western blotting.Neuronal apoptosis in the hippocampus was detected with the transferase-mediated dUTP-X nick end labeling assay.Endogenous neurogenesis was examined with bromodeoxyuridine staining.The MWM test and hippocampal p-CREB,BDNF,and TrkB protein levels in the combined acupoints group were evaluated after the administration of a PKA-selective inhibitor(H89).RESULTS:Spatial learning and memory were significantly impaired in rats with insomnia.The spatial learning deficits were ameliorated in the Shenmen(HT7),Baihui(GV20),Sanyinjiao(SP6),and combined groups;this improvement was significantly greater in the combined group than the single acupoint groups.The spatial memory impairment was improved in the combined,Baihui(GV20),and Shenmen(HT7)groups,but not the Sanyinjiao(SP6)group.The expressions of PKA-Cβ,p-CREB,BDNF,and TrkB were decreased in rats with insomnia.All these proteins were significantly upregulated in the combined group.PKA/p-CREB protein levels were elevated in the Baihui(GV20)and Shenmen(HT7)groups,whereas BDNF/TrkB expression was upregulated in the Sanyinjiao(SP6)group.The staining results showed significant attenuation of hippocampal cell apoptosis and increased numbers of proliferating cells in the combined group,whereas the single acupoint groups only showed decreased numbers of apoptotic cells.In the combined group,the PKA inhibitor reversed the improvement of spatial memory and upregulation of pCREB expression caused by EA,but did not affect its activation of BDNF/TrkB signaling.CONCLUSIONS:EA at the single acupoints Baihui(GV20),Shenmen(HT7),or Sanyinjiao(SP6)had an ameliorating effect on the spatial learning and memory deficits induced by insomnia.EA at combined acupoints exerted a synergistic effect on the improvements in spatial learning and memory impairment in rats with insomnia by upregulating the hippocampal PKA/CREB and BDNF/TrkB signaling,facilitating neurogenesis,and inhibiting neuronal apoptosis.These findings indicate that EA at combined acupoints[(Baihui(GV20),Shenmen(HT7),and Sanyinjiao(SP6)]achieves a more pronounced regulation of hippocampal neuroplasticity than EA at single acupoints,which may partly explain the underlying mechanisms by which EA at combined acupoints exerts a better ameliorative effect on the cognitive dysfunction caused by insomnia.展开更多
Objective:To explore the rules of acupoint combination in acupuncture treatment of post-stroke pseudobulbar palsy(PBP),based on acupuncture prescriptions retrieved from literature and using complex network analysis.Me...Objective:To explore the rules of acupoint combination in acupuncture treatment of post-stroke pseudobulbar palsy(PBP),based on acupuncture prescriptions retrieved from literature and using complex network analysis.Methods:The articles were searched from China National Knowledge Infrastructure(CNKI),Wanfang and VIP databases as the source of data.The eligible articles were selected in accordance with inclusion and exclusion criteria.The relevant data were extracted to set up the acupoint prescription database in acupuncture treatment of PBP.By using co-occurrence relationship in acupoint prescriptions,the core acupoints and the combination rules were analyzed in acupuncture treatment of PBP.Results:A total of 194 articles were eligible finally,with 202 acupoint prescriptions extracted.19 core acupoints were collected in total,i.e.Liánquán(廉泉CV23),Fēngchí(风池GB20),Bǎihuì(百会GV20),Jīnjīn(金津EX-HN12),Yùyè(玉液EX-HN13),Fēngfǔ(风府GV16),Yǎmén(哑门GV15),Shuǐgōu(水沟GV26),Nèiguān(内关PC6),Fēnglóng(丰隆ST40),Wángǔ(完骨GB12),Sānyīnjiāo(三阴交SP16),Yìfēng(翳风TE17),Tōnglǐ(通里HT5),Tàichōng(太冲LR3),Hégǔ(合谷LI4),Hǎiquán(海泉EX-HN11),Jùquán(聚泉EX-HN10)and Yìntáng(印堂EX-HN3).The core acupoint groups were CV23-GB20,EX-HN12-EX-HN13 and GV26-PC6-SP6.Acupoints were mostly selected from governor vessel.The methods of acupoint combination included the combination of distal and nearby points,and the combination of the acupoints located in the front and on the back.Conclusions:In treatment of PBP with acupuncture,biaoben(manifestation and root cause)theory is emphasized,the methods for dispelling wind,resolving phlegm,regaining consciousness,and opening orifices are recommended.Moreover,the attentions are paid on spirit regulation,holistic adjustment,and syndrome differentiation in treatment.展开更多
In the present paper, the author reviews the progresses in the study on acumoxi (acupuncture-moxi-bustion) treatment of diabetes mellitus (DM) from Chinese ancient classical works, Chinese and foreign journals. The th...In the present paper, the author reviews the progresses in the study on acumoxi (acupuncture-moxi-bustion) treatment of diabetes mellitus (DM) from Chinese ancient classical works, Chinese and foreign journals. The therapeutic results reveal that acupuncture treatment of DM is effective in improving DM patients' symptoms via vagal-nerve reflex, regulation of neuroendocrine and immune functions. The author holds that ① correct selection of acupoints in accordance with syndrome differentiation and the related meridians and combined application of acupuncture and Chinese herbal medicines (for oral administration) are the key points for raising the therapeutic effect; and ② acupoint combination and curative methods need to be researched further.展开更多
To point out that ＂analogical acupoint＂ is the acupoints with same indication effect by describing the concept of analogical acupoint, as well as its relationship with combination and prescription. ＂Analogical acup...To point out that ＂analogical acupoint＂ is the acupoints with same indication effect by describing the concept of analogical acupoint, as well as its relationship with combination and prescription. ＂Analogical acupoint＂ is the basic element for acupoint combination, while the acupoint combination is the foundation of acupuncture prescription. Finally, to make future prospect for the research thought of ＂analogical acupoint＂.展开更多
Acupuncture is widely used to treat functional dyspepsia with satisfactory outcomes. Combination of the He and Mu acupoints is commonly used and has a synergistic effect on functional dyspepsia; however, its underlyin...Acupuncture is widely used to treat functional dyspepsia with satisfactory outcomes. Combination of the He and Mu acupoints is commonly used and has a synergistic effect on functional dyspepsia; however, its underlying mechanisms remain unclear. Therefore, a randomized controlled parallel clinical trial is currently underway at Chengdu University of Traditional Chinese Medicine, China. This trial is designed to explore the efficacy of and central responses to the He-Mu point combination in patients with functional dyspepsia using functional magnetic resonance imaging. A total of 105 patients with functional dyspepsia will be allocated into 3 groups： the low-He point group（puncturing at Zusanli（ST36））, Mu point group（puncturing at Zhongwan（CV12））, and He-Mu point combination group（puncturing at ST36 and CV12）. Every participant will receive 20 sessions of manual acupuncture for 4 weeks. The needles will be inserted perpendicularly to a depth of 1 to 2 cun. The angle of rotation and twisting will range from 90 to 180 degrees, while lifting and thrusting will range from 0.3 to 0.5 cm. The various manipulations will be performed 60 to 90 times per minute. The needles will remain in place for 30 minutes, during which manipulation will be applied every 10 minutes. Magnetic resonance imaging will be performed before and after 20 sessions of acupuncture. The primary outcome is symptom improvement according to the Chinese version of the Nepean Dyspepsia Index. Secondary outcomes include the Leeds dyspepsia questionnaire, Self-Rating Anxiety Scale, Self-Rating Depression Scale, Beck Anxiety Inventory, Beck Depression Inventory, and visual analogue scale scores before and after 10 and 20 sessions of acupuncture. Needle sensation and adverse events will be used to assess the therapeutic effects. This study will promote more widespread awareness of the benefits of acupoint combination in the clinical setting and provide a further explanation of the neuromechanism by which acupuncture at the He-Mu point combination for functional dyspepsia. Registration： Chinese Clinical Trial Registry, Chi CTR-IOR-15006402.展开更多
OBJECTIVE: To assess the clinical effect and safety of direct current(DC) pulse produced by Han's Acupoint Nerve Stimulator in reduction(HANS) of labor pain.METHODS: Totally 120 participants were enrolled in this ...OBJECTIVE: To assess the clinical effect and safety of direct current(DC) pulse produced by Han's Acupoint Nerve Stimulator in reduction(HANS) of labor pain.METHODS: Totally 120 participants were enrolled in this clinical trial, and were randomly divided into4 groups including: HANS group, patient controlled intravenous analgesia(PCIA) group, patient-controlled epidural analgesia(PCEA) group and control group. The HANS group was treated by stimulating the acupoints of Jia Ji(T10-L3) and Ciliao(BL 32)with DC pulse of 100 Hz and 15-30 m A produced by a portable battery-powered Han's Acupoint Nerve Stimulator for 30 min. The PCIA group was intravenously infused Ondansetron(8 mg) for 5 min,then tramadol injection(1.5 mg/kg) was slowly dripped by using Baxter APⅡ electronic pump with50 m L tramadol(0.70%) + ondansetron(8 mg),background infusion 2 m L/h, PCA dose of 2 m L,lockout interval of 10 min. In PCEA group, women received intrathecal injection ropivacaine(3 mg) in L2-3, and epidural catheter was connected to Baxter APⅡ electronic pump, with 100 m L Ropivacaine(0.1%) and Sufentanil(50 ug), background infusion5 m L, Patient controlled analgesia(PCA) dose of 5m L, lockout interval of 10 min. The control group was not received analgesia. The visual analogue scale(VAS), stage and manner of labor, Apgar score of newborn, neonatal weights, oxytocin dosage,postpartum hemorrhage and side effects were monitored in all groups.RESULTS: The vital signs were all stable in the four analgesic groups. After analgesia, there was statistical difference in VAS score between HANS group and control group, between PCEA group and the control group, between PCIA group and control group. The analgesic effect in the PCEA group was significantly better than that of other two groups.The second stage of labor in the PCEA group was longer than the other three groups, showing significant difference between them. The Apgar score of newborn 1min after birth in the PCIA group was slightly lower than that of the other two groups,showing significant difference between them. The neonatal weights between four groups were not significantly different. The rate of cesarean sectionin the control group was significantly higher than that of the labor analgesia group, there was statistically difference in four groups. The number of PCIA group that used oxytocin was lower than that of other three groups. There was no significant difference in postpartum hemorrhage between four groups. The side effects of the PCEA group were itching, uroschesis and neonatal asphyxia and PCIA group were nausea and vomiting and neonatal asphyxia. However, fewer side effects were observed in the HANS group.CONCLUSION: The DC pulse produced by HANS may be a non-pharmacological alternative to labor pain with fewer side effects.展开更多
Objective:To explore the clinical application rules of Láogōng(劳宫PC8)by analyzing the comparison and contrast of the practice with this acupoint in ancient times and in present.Methods:Relevant literature befo...Objective:To explore the clinical application rules of Láogōng(劳宫PC8)by analyzing the comparison and contrast of the practice with this acupoint in ancient times and in present.Methods:Relevant literature before October 1,1949 was selected from Chinese Medical Code(Fifth Edition)and Compilation of Moden Chinese Medicine Journals and literature published after October 1,1949 was screened from China National knowledge Infrastructure(CNIK),Wanfang Data Knowledge Service Platform(Wanfang),VIP Chinese Journal Service Platform(VIP),Chinese BioMedine Database(CBM),Pubmed Database,and Web of Science.After screening,SQL Server database was established.The complex network analysis was conducted by using Gephi and the cluster analysis was performed by SPSS Statistics.Results:Before October 1,1949,Lráogōng(劳宫PC8),on a single use or with a match of Dàlíng(大陵PC7),Guānyuán(关元CV4),and Zúsānlǐ(足三里ST36),was mainly used for treating ozostomia and edema due to pregnancy.However,Láogōng(劳宫PC8),on a single use or with a match of Yǒngquán(涌泉KI1),Hégǔ(合谷L14)and Nèiguān(内关PC6)was commonly for the treatment of toothache and the sequelae after wind stroke after October 1 st,1949.Conclusion:Láogōng(劳宫PC8)is mainly used to treat local disorders or diseases pertaining to the pericardium meridian.The single-point therapy was commonly applied before October 1 st,1949,while a combination of other points is more common afterwards and the scope of indication expands,with its application for psychiatry disorders,pain diseases and emergent diseases.Acupoints on the same meridian of Láogōng(劳宫PC8),ying-spring points,the back-shu points and combination between and the upper and the lower are dominated in the selection of supplementary points.The supplementary acupoints with the highest frequency of use were specific acupoints,including five-shu points,yuan-source points,front-mu points,etc。展开更多
Insomnia, a common sleep disorder, affects general well-being, hastens the onset of other diseases, and impairs work performance. Hypnotic medications are efficacious in the short term but have obvious side effects. A...Insomnia, a common sleep disorder, affects general well-being, hastens the onset of other diseases, and impairs work performance. Hypnotic medications are efficacious in the short term but have obvious side effects. Acupuncture, often used to treat insomnia in traditional Chinese medicine (TCM), is considered to be beneficial in restoring the normal sleep-wake cycle by regulating and restoring the natural flow of qi (energy power). The three main TCM theories for treating insomnia by acupuncture are the tranquilization disturbance, zangfu disturbance (disequilibrium of internal organs), and imbalance of yin and yang theories. Moxibustion, another treatment for insomnia, is usually combined with acupuncture. Acupuncture and moxibustion with tuina (exercise massage), acupuncture with Chinese herbal injection, electroacupuncture, and acupuncture with medication or psychotherapy are other interventions. Some acupuncture-based methods such as needle-rolling acupuncture, auricular acupoint plaster therapy, phlebotomy, and acupoint catgut-embedding therapy are used as well. Although most clinical trials have shown that acupuncture and its combination therapies are significantly effective in insomnia, the beneficial effects may have been overvalued, because of small sample size, nonstrict inclusion and exclusion criteria, flawed methodology, short follow-up, or nonstandardized evaluation. Therefore, clinical studies of high methodological quality are needed to verify the efficacy of acupuncture, moxibustion, and other combination therapies in insomnia.展开更多
Objective:To investigate the influencing mechanism of Jin-Shui-Liu-Jun-Jian and Acupoint Application combined therapy on hemorheology, inflammatory factors and pulmonary functions in patients with chronic bronchitis, ...Objective:To investigate the influencing mechanism of Jin-Shui-Liu-Jun-Jian and Acupoint Application combined therapy on hemorheology, inflammatory factors and pulmonary functions in patients with chronic bronchitis, thus to offer relevant help for clinical therapies on patients with chronic bronchitis. Methods:120 cases of patients with chronic bronchitis treated in our hospital were selected and randomly divided to be the therapeutic group and the control group, 60 cases each. Patients in control group were provided some basic treatments, such as spasmolysis, anti-inflammation, asthma relieving, oxygen inhalation and dissolve phlegm. For therapeutic group on this basis, Jin-Shui-Liu-Jun-Jian and Acupoint Application combined therapy was provided. Hemorheology, inflammatory factors and pulmonary functions in the two groups of patients were detected before and after treatment. Results:Differences of hemorheology indexes, inflammatory factors and pulmonary function relevant indexes between the two groups of patients with chronic bronchitis before treatment showed no statistical significance (P>0.05). Compared with prior treatment, hemorheology relevant indexes [whole blood high shear viscosity (WHV), whole blood low shear viscosity (WLV), packed cell volume (PCV), plasma viscosity (PV) and red cell assembling index (RCAI)], inflammatory factors [IL-8 (interleukin-8) and TNF-α(tumor necrosis factor-α)] were significantly decreased and pulmonary function relevant indexes [forced vital capacity (FVC), forced expiratory volume in one second (FEV1.0), peak expiratory flow (PEF), maximal voluntary ventilation (MVV) and expiratory flow rate when exhale vital capacity was 0.50], inflammatory factors (IL-4, IL-2) were significantly increased in the two groups of patients with chronic bronchitis after relevant treatments;The differences between the groups showed statistical significance (P<0.05). After combined therapy provided, pulmonary function relevant indexes (FVC, FEV1.0, PEF, MVV and V0.50) and inflammatory factors (IL-4, IL-2) in therapeutic group were significantly higher than control group after basic therapy. Hemorheology relevant indexes (WHV, WLV, PV, PCV and RCAI) and inflammatory factors (IL-8 and TNF-α) in therapeutic group were significantly lower than control group;Differences between the two groups showed statistical significance (P<0.05). Conclusions: Combination of Jin-Shui-Liu-Jun-Jian and Acupoint Application significantly improved the levels of hemorheology, inflammatory factors and pulmonary functions in patients with chronic bronchitis. It has a great important clinical significance on therapies for patients with chronic bronchitis.展开更多
基金the National 973 Program of China, No. 2006CB504505the National Nature Science Foundation of China, No. 90709027
文摘Acupoint combination is a method used for acupoint treatment of patients. Traditionally, acupoints are matched along the meridian distribution, which is a common rule in clinical practice, but the underlying mechanism remains unclear. Cerebral scans with functional magnetic resonance imaging （fMRI） have been used in the study of acupuncture and acupoint specifically. In this study, fMRI was used to detect the activation of the brain areas under different acupoints, matched along different meridians, to elucidate the acupoint combination via a modern medical approach. Forty healthy volunteers were randomly divided into the following groups： Waiguan point （SJ 5）, Waiguan （SJ 5） ＋ Zhigou （SJ 6） （2 acupoints come from the same meridian）, Waiguan （SJ 5） ＋ Neiguan （PC 6） （2 acupoints come from 2 meridians with the relationship of interior-exterior）, Waiguan （SJ 5） ＋ Yanglingquan （GB 34） （2 acupoints come from 2 meridians with the same name-Shaoyang Meridian）, and sham point groups （needling in different points on the right hand）. A real-time cerebral fMRI scan was simultaneously performed. The cerebral activation rate, and the number and strength of different regions of interest were compared among the groups. The fMRI cerebral imaging confirmed that there were some differences in the activation of cerebral areas by the needlings in SJ 5, and in combination with other acupoints. Needling at SJ 5 alone greatly activated the right cerebellum, while needling at both SJ 5 and different co-needling points activated different cerebral functional areas.
基金Supported by “The 12th five-year plan” programmed project of educational science in Hubei Province:2014B095
文摘Objective To observe the influence of different acupoint combinations on immediate effect of surface electromyography of patients with cervical spondylosis, and to explore the interactions and laws among different acupoint combinations. Methods Acupuncture in three kinds of different acupoint combinations was conducted on 90 patients with cervical spondylosis（three groups）： group A [distal point selection group： Kūnlún（昆仑 BL 60） and Hòuxī（后溪 SI 3）]; group B [local point selection group： Fēngchí（风池 GB 20） and Tiānzhù（天柱 BL 10）]; group C（distal and local point selection group： BL 60, SI 3, GB 20 and BL 10）. Self control before and after treatment was adopted to observe the mean value of surface electromyography（SEMG） amplitude, to measure the mean values of integrated electromyography（IEMG） and electromyography root mean square（RMS）, and to calculate the variation rate of electromyography amplitude of trapezius on the affected side of patients before and after acupuncture. The differences of influences of different acupoint combinations on immediate effect of surface electromyography were compared intra-group, and the differences among the three groups after acupuncture were also compared. Results According to the intragroup comparison before and after acupuncture, the differences of mean value of electromyography amplitude, variation rate of electromyography amplitude, IEMG and RMS were both statistically significant（all P〈0.05）. According to the inter-group comparison after acupuncture, the mean value of electromyography amplitude, variation rate of electromyography amplitude, IEMG and RMS varied in different degrees, however, the differences were not significant（all P〉0.05）; there was no significant difference in the total effective rate among the three groups（P〉0.05）. Conclusion Acupuncture treatment in different acupoint combinations can increase IEMG and RMS and reduce mean value of electromyography amplitude and variation rate of electromyography amplitude, enhance cervical vertebral stability and active contractility of muscle fiber, and improve the fatigue resistance of neck flexion; while, the therapeutic effect of acupuncture on cervical spondylosis has nothing to do with the distance or the number of selected acupoint.
基金Supported by National Natural Science Foundation of China:81473773.
文摘Objective:To analyze the rule of acupoint combination of insomnia treated with modern acupuncturemoxibustion with complex network technology and further reveal the internal characteristics of acupoint combination.Methods:Using computer retrieval,the relevant clinical trials of acupuncture and moxibustion in treatment of insomnia were searched from China National knowledge Infrastructure(CNKI),Wanfang Data Knowledge Service Platform(Wanfang),VIP Chinese Journal Service Platform(VIP) and Pubmed Database in recent 5 years.The articles were screened according to the inclusion and exclusion criteria,and then the database was set up and the acupoint prescriptions of acupuncture and moxibustion were extracted.Using Excel 2016 software,the data were processed and the frequency analysis was conducted.With SPSS Modeler 18.0,the association rules were analyzed.Using Gephi 0.9.2,the community analysis and complex network analysis were conducted.Results:Finally,766 articles were eligible and 1276 acupoint prescriptions were extracted with 186 acupoints involved.The acupoints with the highest frequency of use were Shénmén(神门 HT7),Bǎihuì(百会GV20) and Sānyīnjiāo(三阴交SP6).The meridians with the highest frequency were bladder meridian and the governor vessel.The acupoints located on the head and foot were mostly selected.The specific points involved were the crossing points,five-shu points and yuan-source points.The main core paired points referred to HT7 and SP6,HT7 and GV20 as well as HT7 and Nèiguān(内关PC6).The meridians with the highest confidence level appeared among heart meridian of hand-shaoyin,spleen meridian of foot-taiyin and kidney meridian of foot-shaoyin.The modular analysis of complex network obtained 4 core communities and topology analysis obtained 35 core points.The main prescription was composed of HT7,GV20,SP6,Sìshéncōng(四神聪EX-HN1),PC6,Tàixī(太溪KI3),Zúsānlǐ(足三里ST36),Tàichōng(太冲LR3),Anmián(安眠EX-HN18),Yìntáng(印堂EX-HN3),Zhàohǎi(照海KI6),Shēnmài(申脉BL62) and Fēngchí(风池 GB20).Conclusion:Acupuncture-moxibustion therapy focuses on regulating the mind/spirit of the heart and brain,as well as of five zang organs.The general treatment principles are regaining consciousness and opening the orifice,replenishing yin and reducing yang,nourishing the heart and tranquilizing.The supplementary points are mostly the combination of distal and nearby points and the combination of the acupoints located in the upper and the lower parts of the body.The back-shu points and the front-mu points are generally used,while the crossing point as the specific point is especially applied.All of these summaries provide the reference and guidance for clinical application and scientific research.
基金“973”Program of the Ministry of Science and Technology of China:Research on Acupoint Optimization,Combination and Evaluation Methods(No.2014CB543103)the SelfSelected Research Program from of China Academy of Chinese Medical Sciences:Study on the Mechanism of Central Amygdala Nucleus Mediated Electroacupuncture on Relieving Chronic Pain and Related Aversive Mood(No.ZZ13-YQ-063)。
文摘OBJECTIVE:To investiage the effect of electroacupuncture(EA)at a single acupoint of Shenmen(HT7),Baihui(GV20),Sanyinjiao(SP6)and at combined acupoints of Shenmen(HT7)and Baihui(GV20)and Sanyinjiao(SP6)on the PKA/CREB and BDNF/TrkB signaling,as well as neuroapoptosis and neurogenesis in hippocampus and elucidate the underlying mechanism of single and combined acupoints on ameliorating spatial learning and memory deficits in a rat model of primary insomnia.METHODS:Primary insomnia was modeled by intraperitoneal injection of para-chlorophenylalanine(PCPA)once daily for 2 d.EA was applied at Shenmen(HT7),Baihui(GV20),Sanyinjiao(SP6),or Shenmen(HT7)+Baihui(GV20)+Sanyinjiao(SP6)(combined)for 30 min daily for 4 d.Spatial learning and memory function was evaluated by the Morris water maze(MWM)test.Protein expressions of hippocampal cAMP-dependent protein kinase(PKA)-Cβ,phosphorylated cAMP-responsive element-binding protein(p-CREB),brainderived neurotrophic factor(BDNF),and tyrosine kinase receptor B(TrkB)were evaluated by Western blotting.Neuronal apoptosis in the hippocampus was detected with the transferase-mediated dUTP-X nick end labeling assay.Endogenous neurogenesis was examined with bromodeoxyuridine staining.The MWM test and hippocampal p-CREB,BDNF,and TrkB protein levels in the combined acupoints group were evaluated after the administration of a PKA-selective inhibitor(H89).RESULTS:Spatial learning and memory were significantly impaired in rats with insomnia.The spatial learning deficits were ameliorated in the Shenmen(HT7),Baihui(GV20),Sanyinjiao(SP6),and combined groups;this improvement was significantly greater in the combined group than the single acupoint groups.The spatial memory impairment was improved in the combined,Baihui(GV20),and Shenmen(HT7)groups,but not the Sanyinjiao(SP6)group.The expressions of PKA-Cβ,p-CREB,BDNF,and TrkB were decreased in rats with insomnia.All these proteins were significantly upregulated in the combined group.PKA/p-CREB protein levels were elevated in the Baihui(GV20)and Shenmen(HT7)groups,whereas BDNF/TrkB expression was upregulated in the Sanyinjiao(SP6)group.The staining results showed significant attenuation of hippocampal cell apoptosis and increased numbers of proliferating cells in the combined group,whereas the single acupoint groups only showed decreased numbers of apoptotic cells.In the combined group,the PKA inhibitor reversed the improvement of spatial memory and upregulation of pCREB expression caused by EA,but did not affect its activation of BDNF/TrkB signaling.CONCLUSIONS:EA at the single acupoints Baihui(GV20),Shenmen(HT7),or Sanyinjiao(SP6)had an ameliorating effect on the spatial learning and memory deficits induced by insomnia.EA at combined acupoints exerted a synergistic effect on the improvements in spatial learning and memory impairment in rats with insomnia by upregulating the hippocampal PKA/CREB and BDNF/TrkB signaling,facilitating neurogenesis,and inhibiting neuronal apoptosis.These findings indicate that EA at combined acupoints[(Baihui(GV20),Shenmen(HT7),and Sanyinjiao(SP6)]achieves a more pronounced regulation of hippocampal neuroplasticity than EA at single acupoints,which may partly explain the underlying mechanisms by which EA at combined acupoints exerts a better ameliorative effect on the cognitive dysfunction caused by insomnia.
基金Supported by Qilu Chinese Medicine Academic Heritage Project:[2020]No.132。
文摘Objective:To explore the rules of acupoint combination in acupuncture treatment of post-stroke pseudobulbar palsy(PBP),based on acupuncture prescriptions retrieved from literature and using complex network analysis.Methods:The articles were searched from China National Knowledge Infrastructure(CNKI),Wanfang and VIP databases as the source of data.The eligible articles were selected in accordance with inclusion and exclusion criteria.The relevant data were extracted to set up the acupoint prescription database in acupuncture treatment of PBP.By using co-occurrence relationship in acupoint prescriptions,the core acupoints and the combination rules were analyzed in acupuncture treatment of PBP.Results:A total of 194 articles were eligible finally,with 202 acupoint prescriptions extracted.19 core acupoints were collected in total,i.e.Liánquán(廉泉CV23),Fēngchí(风池GB20),Bǎihuì(百会GV20),Jīnjīn(金津EX-HN12),Yùyè(玉液EX-HN13),Fēngfǔ(风府GV16),Yǎmén(哑门GV15),Shuǐgōu(水沟GV26),Nèiguān(内关PC6),Fēnglóng(丰隆ST40),Wángǔ(完骨GB12),Sānyīnjiāo(三阴交SP16),Yìfēng(翳风TE17),Tōnglǐ(通里HT5),Tàichōng(太冲LR3),Hégǔ(合谷LI4),Hǎiquán(海泉EX-HN11),Jùquán(聚泉EX-HN10)and Yìntáng(印堂EX-HN3).The core acupoint groups were CV23-GB20,EX-HN12-EX-HN13 and GV26-PC6-SP6.Acupoints were mostly selected from governor vessel.The methods of acupoint combination included the combination of distal and nearby points,and the combination of the acupoints located in the front and on the back.Conclusions:In treatment of PBP with acupuncture,biaoben(manifestation and root cause)theory is emphasized,the methods for dispelling wind,resolving phlegm,regaining consciousness,and opening orifices are recommended.Moreover,the attentions are paid on spirit regulation,holistic adjustment,and syndrome differentiation in treatment.
文摘In the present paper, the author reviews the progresses in the study on acumoxi (acupuncture-moxi-bustion) treatment of diabetes mellitus (DM) from Chinese ancient classical works, Chinese and foreign journals. The therapeutic results reveal that acupuncture treatment of DM is effective in improving DM patients' symptoms via vagal-nerve reflex, regulation of neuroendocrine and immune functions. The author holds that ① correct selection of acupoints in accordance with syndrome differentiation and the related meridians and combined application of acupuncture and Chinese herbal medicines (for oral administration) are the key points for raising the therapeutic effect; and ② acupoint combination and curative methods need to be researched further.
基金Supported by Funding project of National Key Fundamental Research Development Planning("973"Plan):2014 CB 543100
文摘To point out that ＂analogical acupoint＂ is the acupoints with same indication effect by describing the concept of analogical acupoint, as well as its relationship with combination and prescription. ＂Analogical acupoint＂ is the basic element for acupoint combination, while the acupoint combination is the foundation of acupuncture prescription. Finally, to make future prospect for the research thought of ＂analogical acupoint＂.
基金supported by the National Scientific Foundation Committee in China,No.81473602the Education Ministry’s New Century Excellent Talents Supporting Plan in China+3 种基金the Foundation for the Author of National Excellent Doctoral Dissertation of China,No.201486the Youth Foundation of Science and Technology Department of Sichuan Province in China,No.15QNJJ0008the National Natural Science Foundation-Excellent Youth Foundation in China,No.81622052the 2011 Co-Innovation Center of Sichuan Province named Acupoint Effects of Acupuncture Co-Innovation Center
文摘Acupuncture is widely used to treat functional dyspepsia with satisfactory outcomes. Combination of the He and Mu acupoints is commonly used and has a synergistic effect on functional dyspepsia; however, its underlying mechanisms remain unclear. Therefore, a randomized controlled parallel clinical trial is currently underway at Chengdu University of Traditional Chinese Medicine, China. This trial is designed to explore the efficacy of and central responses to the He-Mu point combination in patients with functional dyspepsia using functional magnetic resonance imaging. A total of 105 patients with functional dyspepsia will be allocated into 3 groups： the low-He point group（puncturing at Zusanli（ST36））, Mu point group（puncturing at Zhongwan（CV12））, and He-Mu point combination group（puncturing at ST36 and CV12）. Every participant will receive 20 sessions of manual acupuncture for 4 weeks. The needles will be inserted perpendicularly to a depth of 1 to 2 cun. The angle of rotation and twisting will range from 90 to 180 degrees, while lifting and thrusting will range from 0.3 to 0.5 cm. The various manipulations will be performed 60 to 90 times per minute. The needles will remain in place for 30 minutes, during which manipulation will be applied every 10 minutes. Magnetic resonance imaging will be performed before and after 20 sessions of acupuncture. The primary outcome is symptom improvement according to the Chinese version of the Nepean Dyspepsia Index. Secondary outcomes include the Leeds dyspepsia questionnaire, Self-Rating Anxiety Scale, Self-Rating Depression Scale, Beck Anxiety Inventory, Beck Depression Inventory, and visual analogue scale scores before and after 10 and 20 sessions of acupuncture. Needle sensation and adverse events will be used to assess the therapeutic effects. This study will promote more widespread awareness of the benefits of acupoint combination in the clinical setting and provide a further explanation of the neuromechanism by which acupuncture at the He-Mu point combination for functional dyspepsia. Registration： Chinese Clinical Trial Registry, Chi CTR-IOR-15006402.
基金Supported by The Scientific Achievement and Appropriate Technology Extension Project of Beijing Municipal Commission of Health and Family Planning(TG-2014-12)
文摘OBJECTIVE: To assess the clinical effect and safety of direct current(DC) pulse produced by Han's Acupoint Nerve Stimulator in reduction(HANS) of labor pain.METHODS: Totally 120 participants were enrolled in this clinical trial, and were randomly divided into4 groups including: HANS group, patient controlled intravenous analgesia(PCIA) group, patient-controlled epidural analgesia(PCEA) group and control group. The HANS group was treated by stimulating the acupoints of Jia Ji(T10-L3) and Ciliao(BL 32)with DC pulse of 100 Hz and 15-30 m A produced by a portable battery-powered Han's Acupoint Nerve Stimulator for 30 min. The PCIA group was intravenously infused Ondansetron(8 mg) for 5 min,then tramadol injection(1.5 mg/kg) was slowly dripped by using Baxter APⅡ electronic pump with50 m L tramadol(0.70%) + ondansetron(8 mg),background infusion 2 m L/h, PCA dose of 2 m L,lockout interval of 10 min. In PCEA group, women received intrathecal injection ropivacaine(3 mg) in L2-3, and epidural catheter was connected to Baxter APⅡ electronic pump, with 100 m L Ropivacaine(0.1%) and Sufentanil(50 ug), background infusion5 m L, Patient controlled analgesia(PCA) dose of 5m L, lockout interval of 10 min. The control group was not received analgesia. The visual analogue scale(VAS), stage and manner of labor, Apgar score of newborn, neonatal weights, oxytocin dosage,postpartum hemorrhage and side effects were monitored in all groups.RESULTS: The vital signs were all stable in the four analgesic groups. After analgesia, there was statistical difference in VAS score between HANS group and control group, between PCEA group and the control group, between PCIA group and control group. The analgesic effect in the PCEA group was significantly better than that of other two groups.The second stage of labor in the PCEA group was longer than the other three groups, showing significant difference between them. The Apgar score of newborn 1min after birth in the PCIA group was slightly lower than that of the other two groups,showing significant difference between them. The neonatal weights between four groups were not significantly different. The rate of cesarean sectionin the control group was significantly higher than that of the labor analgesia group, there was statistically difference in four groups. The number of PCIA group that used oxytocin was lower than that of other three groups. There was no significant difference in postpartum hemorrhage between four groups. The side effects of the PCEA group were itching, uroschesis and neonatal asphyxia and PCIA group were nausea and vomiting and neonatal asphyxia. However, fewer side effects were observed in the HANS group.CONCLUSION: The DC pulse produced by HANS may be a non-pharmacological alternative to labor pain with fewer side effects.
文摘Objective:To explore the clinical application rules of Láogōng(劳宫PC8)by analyzing the comparison and contrast of the practice with this acupoint in ancient times and in present.Methods:Relevant literature before October 1,1949 was selected from Chinese Medical Code(Fifth Edition)and Compilation of Moden Chinese Medicine Journals and literature published after October 1,1949 was screened from China National knowledge Infrastructure(CNIK),Wanfang Data Knowledge Service Platform(Wanfang),VIP Chinese Journal Service Platform(VIP),Chinese BioMedine Database(CBM),Pubmed Database,and Web of Science.After screening,SQL Server database was established.The complex network analysis was conducted by using Gephi and the cluster analysis was performed by SPSS Statistics.Results:Before October 1,1949,Lráogōng(劳宫PC8),on a single use or with a match of Dàlíng(大陵PC7),Guānyuán(关元CV4),and Zúsānlǐ(足三里ST36),was mainly used for treating ozostomia and edema due to pregnancy.However,Láogōng(劳宫PC8),on a single use or with a match of Yǒngquán(涌泉KI1),Hégǔ(合谷L14)and Nèiguān(内关PC6)was commonly for the treatment of toothache and the sequelae after wind stroke after October 1 st,1949.Conclusion:Láogōng(劳宫PC8)is mainly used to treat local disorders or diseases pertaining to the pericardium meridian.The single-point therapy was commonly applied before October 1 st,1949,while a combination of other points is more common afterwards and the scope of indication expands,with its application for psychiatry disorders,pain diseases and emergent diseases.Acupoints on the same meridian of Láogōng(劳宫PC8),ying-spring points,the back-shu points and combination between and the upper and the lower are dominated in the selection of supplementary points.The supplementary acupoints with the highest frequency of use were specific acupoints,including five-shu points,yuan-source points,front-mu points,etc。
文摘Insomnia, a common sleep disorder, affects general well-being, hastens the onset of other diseases, and impairs work performance. Hypnotic medications are efficacious in the short term but have obvious side effects. Acupuncture, often used to treat insomnia in traditional Chinese medicine (TCM), is considered to be beneficial in restoring the normal sleep-wake cycle by regulating and restoring the natural flow of qi (energy power). The three main TCM theories for treating insomnia by acupuncture are the tranquilization disturbance, zangfu disturbance (disequilibrium of internal organs), and imbalance of yin and yang theories. Moxibustion, another treatment for insomnia, is usually combined with acupuncture. Acupuncture and moxibustion with tuina (exercise massage), acupuncture with Chinese herbal injection, electroacupuncture, and acupuncture with medication or psychotherapy are other interventions. Some acupuncture-based methods such as needle-rolling acupuncture, auricular acupoint plaster therapy, phlebotomy, and acupoint catgut-embedding therapy are used as well. Although most clinical trials have shown that acupuncture and its combination therapies are significantly effective in insomnia, the beneficial effects may have been overvalued, because of small sample size, nonstrict inclusion and exclusion criteria, flawed methodology, short follow-up, or nonstandardized evaluation. Therefore, clinical studies of high methodological quality are needed to verify the efficacy of acupuncture, moxibustion, and other combination therapies in insomnia.
文摘Objective:To investigate the influencing mechanism of Jin-Shui-Liu-Jun-Jian and Acupoint Application combined therapy on hemorheology, inflammatory factors and pulmonary functions in patients with chronic bronchitis, thus to offer relevant help for clinical therapies on patients with chronic bronchitis. Methods:120 cases of patients with chronic bronchitis treated in our hospital were selected and randomly divided to be the therapeutic group and the control group, 60 cases each. Patients in control group were provided some basic treatments, such as spasmolysis, anti-inflammation, asthma relieving, oxygen inhalation and dissolve phlegm. For therapeutic group on this basis, Jin-Shui-Liu-Jun-Jian and Acupoint Application combined therapy was provided. Hemorheology, inflammatory factors and pulmonary functions in the two groups of patients were detected before and after treatment. Results:Differences of hemorheology indexes, inflammatory factors and pulmonary function relevant indexes between the two groups of patients with chronic bronchitis before treatment showed no statistical significance (P>0.05). Compared with prior treatment, hemorheology relevant indexes [whole blood high shear viscosity (WHV), whole blood low shear viscosity (WLV), packed cell volume (PCV), plasma viscosity (PV) and red cell assembling index (RCAI)], inflammatory factors [IL-8 (interleukin-8) and TNF-α(tumor necrosis factor-α)] were significantly decreased and pulmonary function relevant indexes [forced vital capacity (FVC), forced expiratory volume in one second (FEV1.0), peak expiratory flow (PEF), maximal voluntary ventilation (MVV) and expiratory flow rate when exhale vital capacity was 0.50], inflammatory factors (IL-4, IL-2) were significantly increased in the two groups of patients with chronic bronchitis after relevant treatments;The differences between the groups showed statistical significance (P<0.05). After combined therapy provided, pulmonary function relevant indexes (FVC, FEV1.0, PEF, MVV and V0.50) and inflammatory factors (IL-4, IL-2) in therapeutic group were significantly higher than control group after basic therapy. Hemorheology relevant indexes (WHV, WLV, PV, PCV and RCAI) and inflammatory factors (IL-8 and TNF-α) in therapeutic group were significantly lower than control group;Differences between the two groups showed statistical significance (P<0.05). Conclusions: Combination of Jin-Shui-Liu-Jun-Jian and Acupoint Application significantly improved the levels of hemorheology, inflammatory factors and pulmonary functions in patients with chronic bronchitis. It has a great important clinical significance on therapies for patients with chronic bronchitis.
