This paper briefly introduces the wisdom of management contained in the quintessence of the wisdom of Chinese ancient sages ＂The quintessence of wisdom in books of all sorts＂, and explores the application of the tra...This paper briefly introduces the wisdom of management contained in the quintessence of the wisdom of Chinese ancient sages ＂The quintessence of wisdom in books of all sorts＂, and explores the application of the traditional Chinese wisdom in resolving vari- ous dilemmas or crisis in today＇s human society, to help the society, the nation, and the enterprises solve the management problems.展开更多
In order to obtain the intact nucleic acid of virus particles, on the basis of one-stepreleased method, two-step-released method and phages we have observed nucleic acid released from some sorts of insect virus partic...In order to obtain the intact nucleic acid of virus particles, on the basis of one-stepreleased method, two-step-released method and phages we have observed nucleic acid released from some sorts of insect virus particles under electron microscope. It has been found that under the condition of reacting on the urea of low concentration for a long time, the nucleic acid from four sorts of viruses could not only be released directly, but also be observed clearly.展开更多
The majority(up to 90%)of riverine materials is transported from the continent to the ocean mainly in flood events.It is thus crucial to characterize the geochemistry of elements and their flux in river system in orde...The majority(up to 90%)of riverine materials is transported from the continent to the ocean mainly in flood events.It is thus crucial to characterize the geochemistry of elements and their flux in river system in order to better constrain their global biogeochemical cycling and impact on the oceanic ecosystem.However,the geochemical behavior including the distribution,migration and partitioning of typical metal elements amongst diff erent phases,during hydrodynamic flood event remains still to be well explored.Here,we investigated the geochemical behaviors of typical metal elements in dissolved phase and suspended particulate matter collected from a single flood event in the natural Chishui River,Southwest China.The results showed clearly that the geochemistry of metal elements was largely controlled by the hydrodynamic eff ect,of which the diff erent flowrates introduce a natural sorting of diff erent mineral particles transported at diff erent flood stages,depending on their shape,size and density.The maximum concentrations of alkaline and alkaline earth metals(Li,Mg,K,Rb and Sr)in SPM appeared before the flood peak,which was largely controlled by aluminosilicate minerals.However,transition metals(Cr,Mn,Fe,Ni and Cu)showed their abundance peaks lagging behind the flowrate summit,as a result of the late arrival of coarse particles or heavy minerals,evidenced by the mineralogical phase analysis.In addition,the distribution coe fficient(K_(d))between particulate and dissolved loads were lower and stable for soluble alkali/alkaline earth metals which could be aff ected by pH,while higher and fluctuant for transition metals that were largely influenced by SPM content.Overall,the present study reveals clear eff ects of hydrodynamic sorting on the geochemistry of metal elements during the flood event of the natural Chishui River,which should be taken into account when characterizing the riverine flux and their impact on marine ecosystem.展开更多
The particle size distribution plays a crucial role in the transportation and deposition of eroded sediments.Gaining insights into the related sorting mechanism can significantly enhance our understanding of such proc...The particle size distribution plays a crucial role in the transportation and deposition of eroded sediments.Gaining insights into the related sorting mechanism can significantly enhance our understanding of such processes.In this study,sand-covered slopes were examined.A controlled indoor rainfall simulation was conducted on loess slopes with a 12°incline and a rainfall intensity of 1.5 mm/min.These slopes were then covered with sand layers of varying thicknesses—0.5,1.0,and 1.5 cm—to observe their effects.The findings have revealed that as the thickness of the sand cover increases,the content of sediment particles smaller than 0.054 mm decreases.In contrast,the content of particles larger than 0.054 mm increases after the sixth minute of runoff.The eroded sediment was predominantly composed of silt.During the inter-rill erosion stage,runoff primarily transported particles larger than 0.054 mm.However,in the subsequent rill erosion and combined rill and inter-rill erosion stages,the runoff predominantly carried finer particles,smaller than 0.054 mm.Moreover,the presence of the sand layer significantly influenced the size and form of the eroded sediment particles.Initially,during the first 10 min of runoff,the eroded particles were predominantly larger than 0.054 mm.After this period,however,the particle size shifted,with the majority of particles being smaller than 0.054 mm.This study highlights the intricate relationship between sediment sorting,the thickness of sand covers,and the dynamics of sediment transport under rainfall-induced erosion.展开更多
Elucidating the mechanisms underlying community assembly remains a central question in community ecology,especially in aquatic ecosystems disrupted by human activities.Understanding the causes and consequences of comm...Elucidating the mechanisms underlying community assembly remains a central question in community ecology,especially in aquatic ecosystems disrupted by human activities.Understanding the causes and consequences of community responses to changing environment is essential for revealing the ecological effects of anthropogenic disturbances and proposing practical strategies for ecological restoration.While stochastic dispersal and species sorting are known to influence local biological communities,most studies have focused on horizontal dispersal,often neglecting the vertical exchange of organisms between planktonic and sedimentary communities when studying stochastic dispersal.We used a highly disturbed urban river in Beijing as a model system to investigate the relative roles of stochastic dispersal versus species sorting driven by local pollution,as well as two components of stochastic dispersal,vertical exchange and horizontal dispersal,in structuring local bacterial communities.Our integrated analyses of planktonic and sedimentary bacterial communities revealed that,despite different spatial patterns along the river,both types of bacterial communities were primarily shaped by stochastic dispersal processes rather than species sorting influenced by the environmental gradient.Notably,in addition to the effect of horizontal dispersal along the river,the vertical exchange between planktonic and sedimentary bacterial communities significantly contributed to the formation of local communities.These findings suggest that both vertical exchange and horizontal dispersal should be considered when assessing the role of stochastic dispersal in shaping local community structure in microbial communities.展开更多
The need to transport goods across countries and islands has resulted in a high demand for commercial vessels.Owing to such trends,shipyards must efficiently produce ships to reduce production costs.Layout and materia...The need to transport goods across countries and islands has resulted in a high demand for commercial vessels.Owing to such trends,shipyards must efficiently produce ships to reduce production costs.Layout and material flow are among the crucial aspects determining the efficiency of the production at a shipyard.This paper presents the initial design optimization of a shipyard layout using Nondominated Sorting Algorithm-Ⅱ(NSGA-Ⅱ)to find the optimal configuration of workstations in a shipyard layout.The proposed method focuses on simultaneously minimizing two material handling costs,namely work-based material handling and duration-based material handling.NSGA-Ⅱ determines the order of workstations in the shipyard layout.The semiflexible bay structure is then used in the workstation placement process from the sequence formed in NSGA-Ⅱ into a complete design.Considering that this study is a case of multiobjective optimization,the performance for both objectives at each iteration is presented in a 3D graph.Results indicate that after 500 iterations,the optimal configuration yields a work-based MHC of 163670.0 WBM-units and a duration-based MHC of 34750 DBM-units.Starting from a random solution,the efficiency of NSGA-Ⅱ demonstrates significant improvements,achieving a 50.19%reduction in work-based MHC and a 48.58%reduction in duration-based MHC.展开更多
Soft rot is a destructive disease that inflicts significant losses on agricultural production and the economy post-harvest.Biocontrol strategies based on antagonistic microorganisms have a broad application prospect t...Soft rot is a destructive disease that inflicts significant losses on agricultural production and the economy post-harvest.Biocontrol strategies based on antagonistic microorganisms have a broad application prospect to fight against plant pathogens.This study utilized fluorescence-activated droplet sorting(FADS)technology as an alternative to traditional plate culture methods to isolate microorganisms with antagonistic activity against the soft rot pathogen Erwinia carotovora Ecc15.Initially,the culture performance of the FADS platform was evaluated by analyzing bacterial diversity in droplet culture samples and agar plate culture samples,our data showed that droplet culture exhibited higher species richness and diversity than plate culture,and more than 95%of the operational taxonomic units(OTUs)in the droplet samples belonged to the rare biosphere.Additionally,we developed a green fluorescent protein(GFP)-Ecc15-based FADS screening system,which achieved an enrichment ratio of up to 148.Using this system,we successfully screened 32 antagonistic bacteria from rhizosphere soil sample of healthy konjac plants,and some may be novel microbial resources,including the genera Lelliottia,Buttiauxella and Leclercia.Notably,strain D-62 exhibited the strongest antibacterial ability against Ecc15,with an inhibition zone diameter of(20.86±1.56)mm.In vivo experiments conducted on the corms of Amorphophallus konjac demonstrated that strain D-62 could effectively reduce the infection ability of Ecc15 to the corms,indicating that strain D-62 has the potential to be developed as a biocontrol agent.Our findings suggested that the FADS screening system showed a screening efficiency approximately 3×10^(3)times higher than plate screening system,while significantly reducing costs of infrastructure,labor and consumables,it provides theoretical guidance for the screening of other plant pathogen biocontrol bacteria.展开更多
本文聚焦于基于多目标跟踪的课堂人数自动统计这一主题,提出将YOLOv8和SORT (Simple Online and Realtime Tracking)算法相结合的方法。其中,YOLOv8作为先进的单阶段目标检测算法,能迅速识别图像或视频帧中的目标并输出关键信息;SORT算...本文聚焦于基于多目标跟踪的课堂人数自动统计这一主题,提出将YOLOv8和SORT (Simple Online and Realtime Tracking)算法相结合的方法。其中,YOLOv8作为先进的单阶段目标检测算法,能迅速识别图像或视频帧中的目标并输出关键信息;SORT算法借助卡尔曼滤波预测目标位置变化。研究通过运用特定的软硬件配置开展实验,对模型进行训练与验证。该方法旨在实现课堂人数的精准自动统计,满足课堂管理和动态监控的需求,为教师及管理人员提供有力工具。其不仅在算法应用上具有创新性,而且在教育管理实践中具有重要的实用价值,有望对智慧教育领域的课堂管理产生积极深远的影响。This paper focuses on the theme of automatic counting of the number of people in the classroom based on multi-object tracking and proposes a method that combines the YOLOv8 and SORT algorithms. Among them, YOLOv8, as an advanced single-stage target detection algorithm, can quickly identify targets in an image or video frame and output key information. The SORT algorithm predicts the position change of the target with the help of Kalman filtering. The research conducts experiment by using specific software and hardware configurations to train and validate the model. This method aims to achieve accurate automatic counting of the number of people in the classroom, meet the needs of classroom management and dynamic monitoring, and provide a powerful tool for teachers and administrators. It is not only innovative in algorithm application, but also has important practical value in educational management practice, and is expected to have a positive and far-reaching impact on classroom management in the field of smart education.展开更多
煤矿井下安全生产是保障矿工生命安全和能源稳定供应的核心环节,但传统监控方法在检测实时性和准确性方面存在明显不足。针对复杂井下环境中人员入侵识别精度低的问题,研究提出一种基于改进You Only Look Once version 5(YOLOv5)和深度...煤矿井下安全生产是保障矿工生命安全和能源稳定供应的核心环节,但传统监控方法在检测实时性和准确性方面存在明显不足。针对复杂井下环境中人员入侵识别精度低的问题,研究提出一种基于改进You Only Look Once version 5(YOLOv5)和深度简单在线实时跟踪算法的电子围栏入侵检测技术。研究通过嵌入注意力机制增强模型对关键特征的感知能力,并利用扩展卡尔曼滤波与匈牙利算法提升跟踪稳定性。实验结果表明,改进后的模型的识别率最高,且随迭代次数的增加其识别率始终在90%以上。该技术在识别性能上,平均精度均值指标为92.5%,每秒帧数提升至116.7,训练时长缩短至8.5 h,漏检率显著降低。研究表明,该方法在光照不均、煤尘干扰等复杂场景下具备更高的检测精度与实时性。该技术的提出可为煤矿井下智能化安全管理提供有效技术支撑,从而提高煤矿安全生产水平,杜绝生产事故出现。展开更多
Emerging evidence suggests that metabolic signals regulate mitochondrial homeostasis,with mitochondria-derived vesicles(MDVs)serving as a critical link between metabolites and mitochondrial quality control.In a recent...Emerging evidence suggests that metabolic signals regulate mitochondrial homeostasis,with mitochondria-derived vesicles(MDVs)serving as a critical link between metabolites and mitochondrial quality control.In a recent study,Tang et al.uncovered a novel mechanism in which metabolites modulate mitochondrial homeostasis throughβ-hydroxybutyrylation of sorting nexin 9(SNX9),thereby promoting MDV biogenesis[1].展开更多
The somatotopic representation of specific body parts is a well-established spatial organizational principle in the primary somatosensory and motor cortices.
The ongoing coronavirus epidemic,including the novel coronavirus(SARS-CoV-2),continues to pose a significant threat to global public health.Host targets address multiple stages of the viral life cycle and provide dive...The ongoing coronavirus epidemic,including the novel coronavirus(SARS-CoV-2),continues to pose a significant threat to global public health.Host targets address multiple stages of the viral life cycle and provide diverse opportunities for therapeutic interventions.This study identified sorting nexin 10(SNX10)as a facilitator of replication of human coronavirus OC43(HCoV-OC43),underscoring its potential as a novel antiviral target.The knockout of SNX10 significantly suppressed HCoV-OC43 replication both in vivo and in vitro.Immunoprecipitation-mass spectrometry(IP-MS)analysis identified the adaptor protein complex 2 subunitμ1(AP2M1)as a direct interactor of SNX10.Specifically,SNX10 facilitates phosphorylation of the AP2M1,thereby enhancing clathrin-mediated viral endocytosis.Furthermore,subsequent binding and internalization assays revealed that SNX10 knockout significantly inhibits viral entry into host cells.Conversely,the reconstitution of SNX10 fully restored viral entry,thereby confirming the critical and indispensable role of SNX10 in pathogen internalization.Simultaneously,SNX10 was identified as a key factor that promotes endosomal acidification by modulating pH levels,which in turn facilitated the release of the viral genome.Notably,the ablation of SNX10 was found to trigger autophagy activation during infection,thereby maintaining intracellular homeostasis.Additionally,it exerted autonomous antiviral effects through lysosomal degradation pathways.Collectively,these findings demonstrate SNX10 serves as a pivotal regulator of the viral life cycle and underscore its therapeutic potential as a multi-faceted antiviral candidate target capable of simultaneously inhibiting viral internalization,viral genomic release,and hostpathogen equilibrium.展开更多
Modern automated generation control(AGC)is increasingly complex,requiring precise frequency control for stability and operational accuracy.Traditional PID controller optimisation methods often struggle to handle nonli...Modern automated generation control(AGC)is increasingly complex,requiring precise frequency control for stability and operational accuracy.Traditional PID controller optimisation methods often struggle to handle nonlinearities and meet robustness requirements across diverse operational scenarios.This paper introduces an enhanced strategy using a multi-objective optimisation framework and a modified non-dominated sorting genetic algorithm Ⅱ(SNSGA).The proposed model optimises the PID controller by minimising key performance metrics:integration time squared error(ITSE),integration time absolute error(ITAE),and rate of change of deviation(J).This approach balances convergence rate,overshoot,and oscillation dynamics effectively.A fuzzy-based method is employed to select the most suitable solution from the Pareto set.The comparative analysis demonstrates that the SNSGA-based approach offers superior tuning capabilities over traditional NSGA-Ⅱ and other advanced control methods.In a two-area thermal power system without reheat,the SNSGA significantly reduces settling times for frequency deviations:2.94s for Δf_(1) and 4.98s for Δf_(2),marking improvements of 31.6%and 13.4%over NSGA-Ⅱ,respectively.展开更多
This paper introduces an intelligent garbage-handling trolley model based on an STM32 single chip microcomputer as the control core.The device is driven by four independent motors to achieve automatic tracking,automat...This paper introduces an intelligent garbage-handling trolley model based on an STM32 single chip microcomputer as the control core.The device is driven by four independent motors to achieve automatic tracking,automatic obstacle avoidance,and fixed-point docking.Using external execution structure to realize the car without the use of a mechanical arm,complete garbage collection,storage,and uninstall function.On this basis,the type of garbage is marked by color,and the color recognition sensor is applied to realize the type recognition after garbage collection and put into the corresponding unloading point,to realize its intelligent classification function.It can automatically complete the established task autonomously.展开更多
Currently,the international economic situation is becoming increasingly complex,and there is significant downward pressure on the global economy.In recent years,China’s infrastructure sector has experienced rapid gro...Currently,the international economic situation is becoming increasingly complex,and there is significant downward pressure on the global economy.In recent years,China’s infrastructure sector has experienced rapid growth,with the structure of its power engineering business gradually shifting from traditional infrastructure construction to more diversified areas such as production and operation,as well as emergency repairs.As a result,the transformation of mechanized construction in power transmission and transformation projects has become increasingly urgent.This article proposes a post-evaluation model based on game theory to improve comprehensive weighting and fuzzy grey relational projection sorting,which can be used to evaluate the optimal mechanized construction scheme for power transmission and transformation projects.The model begins by considering the entire lifecycle of power transmission and transformation projects.It constructs a post-evaluation index system that covers the planning and design stage,on-site construction stage,operation and maintenance stage,and the decommissioning and disposal stage,with corresponding calculation methods for each index.The fuzzy grey correlation projection sorting method is then employed to evaluate and rank the construction schemes.To validate the model’s effectiveness,a case study of a power transmission and transformation project in a specific region of China is used.The comprehensive benefits of three proposed mechanized construction schemes are evaluated and compared.According to the evaluation results,Scheme 1 is ranked the highest,with a membership degree of 0.870945,excelling in sustainability.These results suggest that the proposed model can effectively evaluate and make decisions regarding the optimal mechanized construction plan for power transmission and transformation projects.展开更多
文摘This paper briefly introduces the wisdom of management contained in the quintessence of the wisdom of Chinese ancient sages ＂The quintessence of wisdom in books of all sorts＂, and explores the application of the traditional Chinese wisdom in resolving vari- ous dilemmas or crisis in today＇s human society, to help the society, the nation, and the enterprises solve the management problems.
文摘In order to obtain the intact nucleic acid of virus particles, on the basis of one-stepreleased method, two-step-released method and phages we have observed nucleic acid released from some sorts of insect virus particles under electron microscope. It has been found that under the condition of reacting on the urea of low concentration for a long time, the nucleic acid from four sorts of viruses could not only be released directly, but also be observed clearly.
基金National Natural Science Foundation of China,42103008,Hongming Cai。
文摘The majority(up to 90%)of riverine materials is transported from the continent to the ocean mainly in flood events.It is thus crucial to characterize the geochemistry of elements and their flux in river system in order to better constrain their global biogeochemical cycling and impact on the oceanic ecosystem.However,the geochemical behavior including the distribution,migration and partitioning of typical metal elements amongst diff erent phases,during hydrodynamic flood event remains still to be well explored.Here,we investigated the geochemical behaviors of typical metal elements in dissolved phase and suspended particulate matter collected from a single flood event in the natural Chishui River,Southwest China.The results showed clearly that the geochemistry of metal elements was largely controlled by the hydrodynamic eff ect,of which the diff erent flowrates introduce a natural sorting of diff erent mineral particles transported at diff erent flood stages,depending on their shape,size and density.The maximum concentrations of alkaline and alkaline earth metals(Li,Mg,K,Rb and Sr)in SPM appeared before the flood peak,which was largely controlled by aluminosilicate minerals.However,transition metals(Cr,Mn,Fe,Ni and Cu)showed their abundance peaks lagging behind the flowrate summit,as a result of the late arrival of coarse particles or heavy minerals,evidenced by the mineralogical phase analysis.In addition,the distribution coe fficient(K_(d))between particulate and dissolved loads were lower and stable for soluble alkali/alkaline earth metals which could be aff ected by pH,while higher and fluctuant for transition metals that were largely influenced by SPM content.Overall,the present study reveals clear eff ects of hydrodynamic sorting on the geochemistry of metal elements during the flood event of the natural Chishui River,which should be taken into account when characterizing the riverine flux and their impact on marine ecosystem.
基金research was funded bymultiple sources,including the Guangxi Natural Science Foundation of Youth Fund(2020GXNSFBA159004)the National Natural Science Foundation Project of China(51779204)the Guilin University of Technology High-Level Talent Research Startup Project(GUTQDJJ2018069).
文摘The particle size distribution plays a crucial role in the transportation and deposition of eroded sediments.Gaining insights into the related sorting mechanism can significantly enhance our understanding of such processes.In this study,sand-covered slopes were examined.A controlled indoor rainfall simulation was conducted on loess slopes with a 12°incline and a rainfall intensity of 1.5 mm/min.These slopes were then covered with sand layers of varying thicknesses—0.5,1.0,and 1.5 cm—to observe their effects.The findings have revealed that as the thickness of the sand cover increases,the content of sediment particles smaller than 0.054 mm decreases.In contrast,the content of particles larger than 0.054 mm increases after the sixth minute of runoff.The eroded sediment was predominantly composed of silt.During the inter-rill erosion stage,runoff primarily transported particles larger than 0.054 mm.However,in the subsequent rill erosion and combined rill and inter-rill erosion stages,the runoff predominantly carried finer particles,smaller than 0.054 mm.Moreover,the presence of the sand layer significantly influenced the size and form of the eroded sediment particles.Initially,during the first 10 min of runoff,the eroded particles were predominantly larger than 0.054 mm.After this period,however,the particle size shifted,with the majority of particles being smaller than 0.054 mm.This study highlights the intricate relationship between sediment sorting,the thickness of sand covers,and the dynamics of sediment transport under rainfall-induced erosion.
基金supported by the National Natural Science Foundation of China(No.32471608)the Open Project of Key Laboratory of Environmental Biotechnology,CAS(No.kf2020002)Yunnan Collaborative Innovation Center for Plateau Lake Ecology and Environmental Health.
文摘Elucidating the mechanisms underlying community assembly remains a central question in community ecology,especially in aquatic ecosystems disrupted by human activities.Understanding the causes and consequences of community responses to changing environment is essential for revealing the ecological effects of anthropogenic disturbances and proposing practical strategies for ecological restoration.While stochastic dispersal and species sorting are known to influence local biological communities,most studies have focused on horizontal dispersal,often neglecting the vertical exchange of organisms between planktonic and sedimentary communities when studying stochastic dispersal.We used a highly disturbed urban river in Beijing as a model system to investigate the relative roles of stochastic dispersal versus species sorting driven by local pollution,as well as two components of stochastic dispersal,vertical exchange and horizontal dispersal,in structuring local bacterial communities.Our integrated analyses of planktonic and sedimentary bacterial communities revealed that,despite different spatial patterns along the river,both types of bacterial communities were primarily shaped by stochastic dispersal processes rather than species sorting influenced by the environmental gradient.Notably,in addition to the effect of horizontal dispersal along the river,the vertical exchange between planktonic and sedimentary bacterial communities significantly contributed to the formation of local communities.These findings suggest that both vertical exchange and horizontal dispersal should be considered when assessing the role of stochastic dispersal in shaping local community structure in microbial communities.
基金Supported by Direktorat Riset dan Pengembangan(Directorate of Research and Development)Universitas Indonesia(NKB-690/UN2.RST/HKP.05.00/2022).
文摘The need to transport goods across countries and islands has resulted in a high demand for commercial vessels.Owing to such trends,shipyards must efficiently produce ships to reduce production costs.Layout and material flow are among the crucial aspects determining the efficiency of the production at a shipyard.This paper presents the initial design optimization of a shipyard layout using Nondominated Sorting Algorithm-Ⅱ(NSGA-Ⅱ)to find the optimal configuration of workstations in a shipyard layout.The proposed method focuses on simultaneously minimizing two material handling costs,namely work-based material handling and duration-based material handling.NSGA-Ⅱ determines the order of workstations in the shipyard layout.The semiflexible bay structure is then used in the workstation placement process from the sequence formed in NSGA-Ⅱ into a complete design.Considering that this study is a case of multiobjective optimization,the performance for both objectives at each iteration is presented in a 3D graph.Results indicate that after 500 iterations,the optimal configuration yields a work-based MHC of 163670.0 WBM-units and a duration-based MHC of 34750 DBM-units.Starting from a random solution,the efficiency of NSGA-Ⅱ demonstrates significant improvements,achieving a 50.19%reduction in work-based MHC and a 48.58%reduction in duration-based MHC.
基金supported by the Guizhou Province High-level Innovative Talent Project(Qiankehe Platform Talent-GCC[2022]027-1)the National Key Research and Development Program of China(2019YFA0904800).
文摘Soft rot is a destructive disease that inflicts significant losses on agricultural production and the economy post-harvest.Biocontrol strategies based on antagonistic microorganisms have a broad application prospect to fight against plant pathogens.This study utilized fluorescence-activated droplet sorting(FADS)technology as an alternative to traditional plate culture methods to isolate microorganisms with antagonistic activity against the soft rot pathogen Erwinia carotovora Ecc15.Initially,the culture performance of the FADS platform was evaluated by analyzing bacterial diversity in droplet culture samples and agar plate culture samples,our data showed that droplet culture exhibited higher species richness and diversity than plate culture,and more than 95%of the operational taxonomic units(OTUs)in the droplet samples belonged to the rare biosphere.Additionally,we developed a green fluorescent protein(GFP)-Ecc15-based FADS screening system,which achieved an enrichment ratio of up to 148.Using this system,we successfully screened 32 antagonistic bacteria from rhizosphere soil sample of healthy konjac plants,and some may be novel microbial resources,including the genera Lelliottia,Buttiauxella and Leclercia.Notably,strain D-62 exhibited the strongest antibacterial ability against Ecc15,with an inhibition zone diameter of(20.86±1.56)mm.In vivo experiments conducted on the corms of Amorphophallus konjac demonstrated that strain D-62 could effectively reduce the infection ability of Ecc15 to the corms,indicating that strain D-62 has the potential to be developed as a biocontrol agent.Our findings suggested that the FADS screening system showed a screening efficiency approximately 3×10^(3)times higher than plate screening system,while significantly reducing costs of infrastructure,labor and consumables,it provides theoretical guidance for the screening of other plant pathogen biocontrol bacteria.
文摘本文聚焦于基于多目标跟踪的课堂人数自动统计这一主题,提出将YOLOv8和SORT (Simple Online and Realtime Tracking)算法相结合的方法。其中,YOLOv8作为先进的单阶段目标检测算法,能迅速识别图像或视频帧中的目标并输出关键信息;SORT算法借助卡尔曼滤波预测目标位置变化。研究通过运用特定的软硬件配置开展实验,对模型进行训练与验证。该方法旨在实现课堂人数的精准自动统计,满足课堂管理和动态监控的需求,为教师及管理人员提供有力工具。其不仅在算法应用上具有创新性,而且在教育管理实践中具有重要的实用价值,有望对智慧教育领域的课堂管理产生积极深远的影响。This paper focuses on the theme of automatic counting of the number of people in the classroom based on multi-object tracking and proposes a method that combines the YOLOv8 and SORT algorithms. Among them, YOLOv8, as an advanced single-stage target detection algorithm, can quickly identify targets in an image or video frame and output key information. The SORT algorithm predicts the position change of the target with the help of Kalman filtering. The research conducts experiment by using specific software and hardware configurations to train and validate the model. This method aims to achieve accurate automatic counting of the number of people in the classroom, meet the needs of classroom management and dynamic monitoring, and provide a powerful tool for teachers and administrators. It is not only innovative in algorithm application, but also has important practical value in educational management practice, and is expected to have a positive and far-reaching impact on classroom management in the field of smart education.
文摘煤矿井下安全生产是保障矿工生命安全和能源稳定供应的核心环节,但传统监控方法在检测实时性和准确性方面存在明显不足。针对复杂井下环境中人员入侵识别精度低的问题,研究提出一种基于改进You Only Look Once version 5(YOLOv5)和深度简单在线实时跟踪算法的电子围栏入侵检测技术。研究通过嵌入注意力机制增强模型对关键特征的感知能力,并利用扩展卡尔曼滤波与匈牙利算法提升跟踪稳定性。实验结果表明,改进后的模型的识别率最高,且随迭代次数的增加其识别率始终在90%以上。该技术在识别性能上,平均精度均值指标为92.5%,每秒帧数提升至116.7,训练时长缩短至8.5 h,漏检率显著降低。研究表明,该方法在光照不均、煤尘干扰等复杂场景下具备更高的检测精度与实时性。该技术的提出可为煤矿井下智能化安全管理提供有效技术支撑,从而提高煤矿安全生产水平,杜绝生产事故出现。
基金supported by research grants from the National Natural Science Foundation of China(32470795)the Guangdong Basic and Applied Basic Research Foundation(2025A1515010506 and 2022A1515111047)+4 种基金the Guangzhou Basic and Applied Basic Research Foundation(2023A04J1951)the Fundamental Research Funds for the Central UniversitiesSun Yat-sen University(24pnpy249)supported by the following research grants from University of Macao(CPG2025-0031-FHS and UM-MYRG2020-00022-FHS)the Macao Science and Technology Development Fund(FDCT0078/2020/A2,FDCT0031/2021/A1,FDCT0081/2022/AMJ,and FDCT 0004/2021/AKP)。
文摘Emerging evidence suggests that metabolic signals regulate mitochondrial homeostasis,with mitochondria-derived vesicles(MDVs)serving as a critical link between metabolites and mitochondrial quality control.In a recent study,Tang et al.uncovered a novel mechanism in which metabolites modulate mitochondrial homeostasis throughβ-hydroxybutyrylation of sorting nexin 9(SNX9),thereby promoting MDV biogenesis[1].
文摘The somatotopic representation of specific body parts is a well-established spatial organizational principle in the primary somatosensory and motor cortices.
基金supported by the National Natural Science Foundation(82130101)the Major Scientific and Technological Projects of Guangdong Province(2023B1111050008)the Post Scientist Fund awarded by the Chinese Academy of Traditional Chinese Medicine(ZZ13035-02)to Shuwen Liu.Regional Joint Foundation of Basic and Applied Basic Research in Guangdong Province(2023A1515111165)to Chan Yang.
文摘The ongoing coronavirus epidemic,including the novel coronavirus(SARS-CoV-2),continues to pose a significant threat to global public health.Host targets address multiple stages of the viral life cycle and provide diverse opportunities for therapeutic interventions.This study identified sorting nexin 10(SNX10)as a facilitator of replication of human coronavirus OC43(HCoV-OC43),underscoring its potential as a novel antiviral target.The knockout of SNX10 significantly suppressed HCoV-OC43 replication both in vivo and in vitro.Immunoprecipitation-mass spectrometry(IP-MS)analysis identified the adaptor protein complex 2 subunitμ1(AP2M1)as a direct interactor of SNX10.Specifically,SNX10 facilitates phosphorylation of the AP2M1,thereby enhancing clathrin-mediated viral endocytosis.Furthermore,subsequent binding and internalization assays revealed that SNX10 knockout significantly inhibits viral entry into host cells.Conversely,the reconstitution of SNX10 fully restored viral entry,thereby confirming the critical and indispensable role of SNX10 in pathogen internalization.Simultaneously,SNX10 was identified as a key factor that promotes endosomal acidification by modulating pH levels,which in turn facilitated the release of the viral genome.Notably,the ablation of SNX10 was found to trigger autophagy activation during infection,thereby maintaining intracellular homeostasis.Additionally,it exerted autonomous antiviral effects through lysosomal degradation pathways.Collectively,these findings demonstrate SNX10 serves as a pivotal regulator of the viral life cycle and underscore its therapeutic potential as a multi-faceted antiviral candidate target capable of simultaneously inhibiting viral internalization,viral genomic release,and hostpathogen equilibrium.
基金supported in part by the Science and Technology Innovation Program of Hunan Province under Grant 2022RC4028in part by the National Natural Science Foundation of China under Grant 62473204+3 种基金in part by the Chunhui Program Collaborative Scientific Research Project under Grant 202202004in part by the Natural Science Foundation of Nanjing University of Posts and Telecommunications under Grants NY221082,NY222144,and NY223075in part by the Huali Program for Excellent Talents in Nanjing University of Posts and Telecommunicationsin part by the Postgraduate Research and Practice Innovation Program of Jiangsu Province under Grant KYCX24_1215.
文摘Modern automated generation control(AGC)is increasingly complex,requiring precise frequency control for stability and operational accuracy.Traditional PID controller optimisation methods often struggle to handle nonlinearities and meet robustness requirements across diverse operational scenarios.This paper introduces an enhanced strategy using a multi-objective optimisation framework and a modified non-dominated sorting genetic algorithm Ⅱ(SNSGA).The proposed model optimises the PID controller by minimising key performance metrics:integration time squared error(ITSE),integration time absolute error(ITAE),and rate of change of deviation(J).This approach balances convergence rate,overshoot,and oscillation dynamics effectively.A fuzzy-based method is employed to select the most suitable solution from the Pareto set.The comparative analysis demonstrates that the SNSGA-based approach offers superior tuning capabilities over traditional NSGA-Ⅱ and other advanced control methods.In a two-area thermal power system without reheat,the SNSGA significantly reduces settling times for frequency deviations:2.94s for Δf_(1) and 4.98s for Δf_(2),marking improvements of 31.6%and 13.4%over NSGA-Ⅱ,respectively.
文摘This paper introduces an intelligent garbage-handling trolley model based on an STM32 single chip microcomputer as the control core.The device is driven by four independent motors to achieve automatic tracking,automatic obstacle avoidance,and fixed-point docking.Using external execution structure to realize the car without the use of a mechanical arm,complete garbage collection,storage,and uninstall function.On this basis,the type of garbage is marked by color,and the color recognition sensor is applied to realize the type recognition after garbage collection and put into the corresponding unloading point,to realize its intelligent classification function.It can automatically complete the established task autonomously.
文摘Currently,the international economic situation is becoming increasingly complex,and there is significant downward pressure on the global economy.In recent years,China’s infrastructure sector has experienced rapid growth,with the structure of its power engineering business gradually shifting from traditional infrastructure construction to more diversified areas such as production and operation,as well as emergency repairs.As a result,the transformation of mechanized construction in power transmission and transformation projects has become increasingly urgent.This article proposes a post-evaluation model based on game theory to improve comprehensive weighting and fuzzy grey relational projection sorting,which can be used to evaluate the optimal mechanized construction scheme for power transmission and transformation projects.The model begins by considering the entire lifecycle of power transmission and transformation projects.It constructs a post-evaluation index system that covers the planning and design stage,on-site construction stage,operation and maintenance stage,and the decommissioning and disposal stage,with corresponding calculation methods for each index.The fuzzy grey correlation projection sorting method is then employed to evaluate and rank the construction schemes.To validate the model’s effectiveness,a case study of a power transmission and transformation project in a specific region of China is used.The comprehensive benefits of three proposed mechanized construction schemes are evaluated and compared.According to the evaluation results,Scheme 1 is ranked the highest,with a membership degree of 0.870945,excelling in sustainability.These results suggest that the proposed model can effectively evaluate and make decisions regarding the optimal mechanized construction plan for power transmission and transformation projects.
