This paper briefly introduces the wisdom of management contained in the quintessence of the wisdom of Chinese ancient sages ＂The quintessence of wisdom in books of all sorts＂, and explores the application of the tra...This paper briefly introduces the wisdom of management contained in the quintessence of the wisdom of Chinese ancient sages ＂The quintessence of wisdom in books of all sorts＂, and explores the application of the traditional Chinese wisdom in resolving vari- ous dilemmas or crisis in today＇s human society, to help the society, the nation, and the enterprises solve the management problems.展开更多
鱼群多目标准确计数是水生态智能监测和集约化养殖产业中的重要环节,对水域生态环境智能保护和水产养殖现代化具有重要作用。现有鱼群多目标准确追踪和计数方法主要适用于鱼群外观清晰、游速缓慢和方向稳定等较理想的情况,难以有效适用...鱼群多目标准确计数是水生态智能监测和集约化养殖产业中的重要环节,对水域生态环境智能保护和水产养殖现代化具有重要作用。现有鱼群多目标准确追踪和计数方法主要适用于鱼群外观清晰、游速缓慢和方向稳定等较理想的情况,难以有效适用于现实情况下存在的鱼群互相遮挡、游动迅速和方向多变等复杂情况。为此,结合轻量化目标检测模型YOLOv5n,提出基于水平相似度匹配机制的鱼群追踪与计数方法。将鱼群计数问题视为多目标检测与追踪问题,设计水平相似度匹配机制,并对SORT(Simple Online and Realtime Tracking)算法进行优化。通过高速水流中鱼群个体在帧与帧之间的位置关系对检测框中心点的水平距离进行限制,以有效解决SORT算法存在的目标匹配混乱问题,显著提高追踪效果。实验结果表明,所提方法在鱼群多目标追踪数据集上的性能显著优于现有追踪方法,对目标遮挡、方向变化等情况目标追踪性能提升显著,并且该方法结构简单,易于实际应用。展开更多
In order to obtain the intact nucleic acid of virus particles, on the basis of one-stepreleased method, two-step-released method and phages we have observed nucleic acid released from some sorts of insect virus partic...In order to obtain the intact nucleic acid of virus particles, on the basis of one-stepreleased method, two-step-released method and phages we have observed nucleic acid released from some sorts of insect virus particles under electron microscope. It has been found that under the condition of reacting on the urea of low concentration for a long time, the nucleic acid from four sorts of viruses could not only be released directly, but also be observed clearly.展开更多
Acute ischemic stroke remains a significant health concern owing to the limited efficacy of current therapeutic options.In recent years,Neuregulin-1 has exhibited promising neuroprotective effects in cerebral ischemia...Acute ischemic stroke remains a significant health concern owing to the limited efficacy of current therapeutic options.In recent years,Neuregulin-1 has exhibited promising neuroprotective effects in cerebral ischemia.However,the sources and functions of Neuregulin-1 have not yet been fully understood,which hinders its translation and broad application.Here,we collected paired clot and peripheral blood samples from patients with acute ischemic stroke to determine the sources of Neuregulin-1.In addition,we established an in vivo transient middle cerebral artery occlusion mouse model to investigate the therapeutic effects of Neuregulin-1 and its underlying molecular biological mechanisms.We observed a significant elevation in serum Neuregulin-1 levels among patients with acute ischemic stroke that correlated with severity of neurological impairment and clinical outcome.Using single-cell sequencing,we identified Neuregulin-1-positive macrophages among peripheral blood mononuclear cells that produced Neuregulin-1 post-ischemia.In addition,Neuregulin-1 promoted repair of the infarcted area,alleviating neuronal and myelin damage and improving overall behavioral recovery in mice.We found that Neuregulin-1 may exert these neuroprotective effects by promoting angiogenesis in the infarct area,and that this effect is mediated by Akt/mTOR/VEGF-dependent signaling.Our findings suggest that peripheral macrophages are a source of Neuregulin-1 post-stroke.Neuregulin-1 exerts its neuroprotective effects by promoting angiogenesis via Akt/mTOR/VEGF-dependent signaling,showing promising clinical translation potential.展开更多
Manned aerial vehicle-unmanned aerial vehicle(MAV-UAV)combat organization is a MAV-UAV combat collective formed from the perspective of organization design theory and methodology,and the generation of force formation ...Manned aerial vehicle-unmanned aerial vehicle(MAV-UAV)combat organization is a MAV-UAV combat collective formed from the perspective of organization design theory and methodology,and the generation of force formation plan is a key step in the organizational planning.Based on the description of the problem and the definition of organizational elements,the matching model of platform-target attack wave is constructed to minimize the redundancy of command and decision-making capability,resource capability and the number of platforms used.Based on the non-dominated sorting genetic algorithmⅢ(NSGA-Ⅲ)framework,which includes encoding/decoding method and constraint handling method,the generation model of organizational force formation plan is solved,and the effectiveness and superiority of the algorithm are verified by simulation experiments.展开更多
Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth...Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth research on related molecular mechanisms is of great significance.Methods:Postpartum mice were anesthetized with pentobarbital and administered lipopolysaccharide to develop the mouse mastitis model.Proteomic analysis was per-formed to compare protein expression in mitochondria-associated endoplasmic retic-ulum membranes(MAM)from two mouse mammary gland groups.Western blot was used to detect the expression of MAM-related proteins in mitochondria.AlphaFold3 was used to predict the molecular structures of phosphofurin acidic cluster sorting protein 2(PACS2)and mitofusin 2(MFN2)and their interaction levels.The MFN2-PACS2 interaction was investigated using co-immunoprecipitation and small interfer-ing RNA.Results:The results showed that the inflammation level in the mammary gland tissue of mice with mastitis significantly increased,the total antioxidant capacity decreased,and the expression of MAM-related proteins MFN2 and PACS2 was significantly downregulated.In cell experiments,overexpression of MFN2 can inhibit inflamma-tion and oxidative stress responses,and promote the interaction between MFN2 and PACS2 to affect the formation of MAMs.Conclusion:In summary,this study suggests that mastitis can alter the expression of MAM-related proteins in mouse breast tissue.The interaction between MFN2 and PACS2 regulates the formation of MAMs.Overexpression of MFN2 can promote the formation of MAMs and inhibit inflammation and oxidative stress response in mam-mary epithelial cells.Our results provided a new theoretical basis and potential thera-peutic targets for the prevention and treatment of mastitis.展开更多
The majority(up to 90%)of riverine materials is transported from the continent to the ocean mainly in flood events.It is thus crucial to characterize the geochemistry of elements and their flux in river system in orde...The majority(up to 90%)of riverine materials is transported from the continent to the ocean mainly in flood events.It is thus crucial to characterize the geochemistry of elements and their flux in river system in order to better constrain their global biogeochemical cycling and impact on the oceanic ecosystem.However,the geochemical behavior including the distribution,migration and partitioning of typical metal elements amongst diff erent phases,during hydrodynamic flood event remains still to be well explored.Here,we investigated the geochemical behaviors of typical metal elements in dissolved phase and suspended particulate matter collected from a single flood event in the natural Chishui River,Southwest China.The results showed clearly that the geochemistry of metal elements was largely controlled by the hydrodynamic eff ect,of which the diff erent flowrates introduce a natural sorting of diff erent mineral particles transported at diff erent flood stages,depending on their shape,size and density.The maximum concentrations of alkaline and alkaline earth metals(Li,Mg,K,Rb and Sr)in SPM appeared before the flood peak,which was largely controlled by aluminosilicate minerals.However,transition metals(Cr,Mn,Fe,Ni and Cu)showed their abundance peaks lagging behind the flowrate summit,as a result of the late arrival of coarse particles or heavy minerals,evidenced by the mineralogical phase analysis.In addition,the distribution coe fficient(K_(d))between particulate and dissolved loads were lower and stable for soluble alkali/alkaline earth metals which could be aff ected by pH,while higher and fluctuant for transition metals that were largely influenced by SPM content.Overall,the present study reveals clear eff ects of hydrodynamic sorting on the geochemistry of metal elements during the flood event of the natural Chishui River,which should be taken into account when characterizing the riverine flux and their impact on marine ecosystem.展开更多
The particle size distribution plays a crucial role in the transportation and deposition of eroded sediments.Gaining insights into the related sorting mechanism can significantly enhance our understanding of such proc...The particle size distribution plays a crucial role in the transportation and deposition of eroded sediments.Gaining insights into the related sorting mechanism can significantly enhance our understanding of such processes.In this study,sand-covered slopes were examined.A controlled indoor rainfall simulation was conducted on loess slopes with a 12°incline and a rainfall intensity of 1.5 mm/min.These slopes were then covered with sand layers of varying thicknesses—0.5,1.0,and 1.5 cm—to observe their effects.The findings have revealed that as the thickness of the sand cover increases,the content of sediment particles smaller than 0.054 mm decreases.In contrast,the content of particles larger than 0.054 mm increases after the sixth minute of runoff.The eroded sediment was predominantly composed of silt.During the inter-rill erosion stage,runoff primarily transported particles larger than 0.054 mm.However,in the subsequent rill erosion and combined rill and inter-rill erosion stages,the runoff predominantly carried finer particles,smaller than 0.054 mm.Moreover,the presence of the sand layer significantly influenced the size and form of the eroded sediment particles.Initially,during the first 10 min of runoff,the eroded particles were predominantly larger than 0.054 mm.After this period,however,the particle size shifted,with the majority of particles being smaller than 0.054 mm.This study highlights the intricate relationship between sediment sorting,the thickness of sand covers,and the dynamics of sediment transport under rainfall-induced erosion.展开更多
Elucidating the mechanisms underlying community assembly remains a central question in community ecology,especially in aquatic ecosystems disrupted by human activities.Understanding the causes and consequences of comm...Elucidating the mechanisms underlying community assembly remains a central question in community ecology,especially in aquatic ecosystems disrupted by human activities.Understanding the causes and consequences of community responses to changing environment is essential for revealing the ecological effects of anthropogenic disturbances and proposing practical strategies for ecological restoration.While stochastic dispersal and species sorting are known to influence local biological communities,most studies have focused on horizontal dispersal,often neglecting the vertical exchange of organisms between planktonic and sedimentary communities when studying stochastic dispersal.We used a highly disturbed urban river in Beijing as a model system to investigate the relative roles of stochastic dispersal versus species sorting driven by local pollution,as well as two components of stochastic dispersal,vertical exchange and horizontal dispersal,in structuring local bacterial communities.Our integrated analyses of planktonic and sedimentary bacterial communities revealed that,despite different spatial patterns along the river,both types of bacterial communities were primarily shaped by stochastic dispersal processes rather than species sorting influenced by the environmental gradient.Notably,in addition to the effect of horizontal dispersal along the river,the vertical exchange between planktonic and sedimentary bacterial communities significantly contributed to the formation of local communities.These findings suggest that both vertical exchange and horizontal dispersal should be considered when assessing the role of stochastic dispersal in shaping local community structure in microbial communities.展开更多
The need to transport goods across countries and islands has resulted in a high demand for commercial vessels.Owing to such trends,shipyards must efficiently produce ships to reduce production costs.Layout and materia...The need to transport goods across countries and islands has resulted in a high demand for commercial vessels.Owing to such trends,shipyards must efficiently produce ships to reduce production costs.Layout and material flow are among the crucial aspects determining the efficiency of the production at a shipyard.This paper presents the initial design optimization of a shipyard layout using Nondominated Sorting Algorithm-Ⅱ(NSGA-Ⅱ)to find the optimal configuration of workstations in a shipyard layout.The proposed method focuses on simultaneously minimizing two material handling costs,namely work-based material handling and duration-based material handling.NSGA-Ⅱ determines the order of workstations in the shipyard layout.The semiflexible bay structure is then used in the workstation placement process from the sequence formed in NSGA-Ⅱ into a complete design.Considering that this study is a case of multiobjective optimization,the performance for both objectives at each iteration is presented in a 3D graph.Results indicate that after 500 iterations,the optimal configuration yields a work-based MHC of 163670.0 WBM-units and a duration-based MHC of 34750 DBM-units.Starting from a random solution,the efficiency of NSGA-Ⅱ demonstrates significant improvements,achieving a 50.19%reduction in work-based MHC and a 48.58%reduction in duration-based MHC.展开更多
Soft rot is a destructive disease that inflicts significant losses on agricultural production and the economy post-harvest.Biocontrol strategies based on antagonistic microorganisms have a broad application prospect t...Soft rot is a destructive disease that inflicts significant losses on agricultural production and the economy post-harvest.Biocontrol strategies based on antagonistic microorganisms have a broad application prospect to fight against plant pathogens.This study utilized fluorescence-activated droplet sorting(FADS)technology as an alternative to traditional plate culture methods to isolate microorganisms with antagonistic activity against the soft rot pathogen Erwinia carotovora Ecc15.Initially,the culture performance of the FADS platform was evaluated by analyzing bacterial diversity in droplet culture samples and agar plate culture samples,our data showed that droplet culture exhibited higher species richness and diversity than plate culture,and more than 95%of the operational taxonomic units(OTUs)in the droplet samples belonged to the rare biosphere.Additionally,we developed a green fluorescent protein(GFP)-Ecc15-based FADS screening system,which achieved an enrichment ratio of up to 148.Using this system,we successfully screened 32 antagonistic bacteria from rhizosphere soil sample of healthy konjac plants,and some may be novel microbial resources,including the genera Lelliottia,Buttiauxella and Leclercia.Notably,strain D-62 exhibited the strongest antibacterial ability against Ecc15,with an inhibition zone diameter of(20.86±1.56)mm.In vivo experiments conducted on the corms of Amorphophallus konjac demonstrated that strain D-62 could effectively reduce the infection ability of Ecc15 to the corms,indicating that strain D-62 has the potential to be developed as a biocontrol agent.Our findings suggested that the FADS screening system showed a screening efficiency approximately 3×10^(3)times higher than plate screening system,while significantly reducing costs of infrastructure,labor and consumables,it provides theoretical guidance for the screening of other plant pathogen biocontrol bacteria.展开更多
本文聚焦于基于多目标跟踪的课堂人数自动统计这一主题,提出将YOLOv8和SORT (Simple Online and Realtime Tracking)算法相结合的方法。其中,YOLOv8作为先进的单阶段目标检测算法,能迅速识别图像或视频帧中的目标并输出关键信息;SORT算...本文聚焦于基于多目标跟踪的课堂人数自动统计这一主题,提出将YOLOv8和SORT (Simple Online and Realtime Tracking)算法相结合的方法。其中,YOLOv8作为先进的单阶段目标检测算法,能迅速识别图像或视频帧中的目标并输出关键信息;SORT算法借助卡尔曼滤波预测目标位置变化。研究通过运用特定的软硬件配置开展实验,对模型进行训练与验证。该方法旨在实现课堂人数的精准自动统计,满足课堂管理和动态监控的需求,为教师及管理人员提供有力工具。其不仅在算法应用上具有创新性,而且在教育管理实践中具有重要的实用价值,有望对智慧教育领域的课堂管理产生积极深远的影响。This paper focuses on the theme of automatic counting of the number of people in the classroom based on multi-object tracking and proposes a method that combines the YOLOv8 and SORT algorithms. Among them, YOLOv8, as an advanced single-stage target detection algorithm, can quickly identify targets in an image or video frame and output key information. The SORT algorithm predicts the position change of the target with the help of Kalman filtering. The research conducts experiment by using specific software and hardware configurations to train and validate the model. This method aims to achieve accurate automatic counting of the number of people in the classroom, meet the needs of classroom management and dynamic monitoring, and provide a powerful tool for teachers and administrators. It is not only innovative in algorithm application, but also has important practical value in educational management practice, and is expected to have a positive and far-reaching impact on classroom management in the field of smart education.展开更多
煤矿井下安全生产是保障矿工生命安全和能源稳定供应的核心环节,但传统监控方法在检测实时性和准确性方面存在明显不足。针对复杂井下环境中人员入侵识别精度低的问题,研究提出一种基于改进You Only Look Once version 5(YOLOv5)和深度...煤矿井下安全生产是保障矿工生命安全和能源稳定供应的核心环节,但传统监控方法在检测实时性和准确性方面存在明显不足。针对复杂井下环境中人员入侵识别精度低的问题,研究提出一种基于改进You Only Look Once version 5(YOLOv5)和深度简单在线实时跟踪算法的电子围栏入侵检测技术。研究通过嵌入注意力机制增强模型对关键特征的感知能力,并利用扩展卡尔曼滤波与匈牙利算法提升跟踪稳定性。实验结果表明,改进后的模型的识别率最高,且随迭代次数的增加其识别率始终在90%以上。该技术在识别性能上,平均精度均值指标为92.5%,每秒帧数提升至116.7,训练时长缩短至8.5 h,漏检率显著降低。研究表明,该方法在光照不均、煤尘干扰等复杂场景下具备更高的检测精度与实时性。该技术的提出可为煤矿井下智能化安全管理提供有效技术支撑,从而提高煤矿安全生产水平,杜绝生产事故出现。展开更多
文摘This paper briefly introduces the wisdom of management contained in the quintessence of the wisdom of Chinese ancient sages ＂The quintessence of wisdom in books of all sorts＂, and explores the application of the traditional Chinese wisdom in resolving vari- ous dilemmas or crisis in today＇s human society, to help the society, the nation, and the enterprises solve the management problems.
文摘鱼群多目标准确计数是水生态智能监测和集约化养殖产业中的重要环节,对水域生态环境智能保护和水产养殖现代化具有重要作用。现有鱼群多目标准确追踪和计数方法主要适用于鱼群外观清晰、游速缓慢和方向稳定等较理想的情况,难以有效适用于现实情况下存在的鱼群互相遮挡、游动迅速和方向多变等复杂情况。为此,结合轻量化目标检测模型YOLOv5n,提出基于水平相似度匹配机制的鱼群追踪与计数方法。将鱼群计数问题视为多目标检测与追踪问题,设计水平相似度匹配机制,并对SORT(Simple Online and Realtime Tracking)算法进行优化。通过高速水流中鱼群个体在帧与帧之间的位置关系对检测框中心点的水平距离进行限制,以有效解决SORT算法存在的目标匹配混乱问题,显著提高追踪效果。实验结果表明,所提方法在鱼群多目标追踪数据集上的性能显著优于现有追踪方法,对目标遮挡、方向变化等情况目标追踪性能提升显著,并且该方法结构简单,易于实际应用。
文摘In order to obtain the intact nucleic acid of virus particles, on the basis of one-stepreleased method, two-step-released method and phages we have observed nucleic acid released from some sorts of insect virus particles under electron microscope. It has been found that under the condition of reacting on the urea of low concentration for a long time, the nucleic acid from four sorts of viruses could not only be released directly, but also be observed clearly.
基金Chongqing Technology lnnovation and Application Development Program,No.CSTB2023TIAD-KPX0061(to ZZ)the National Natural Science Foundation of China,Nos.81971130(to ZZ),82201464(to XC).
文摘Acute ischemic stroke remains a significant health concern owing to the limited efficacy of current therapeutic options.In recent years,Neuregulin-1 has exhibited promising neuroprotective effects in cerebral ischemia.However,the sources and functions of Neuregulin-1 have not yet been fully understood,which hinders its translation and broad application.Here,we collected paired clot and peripheral blood samples from patients with acute ischemic stroke to determine the sources of Neuregulin-1.In addition,we established an in vivo transient middle cerebral artery occlusion mouse model to investigate the therapeutic effects of Neuregulin-1 and its underlying molecular biological mechanisms.We observed a significant elevation in serum Neuregulin-1 levels among patients with acute ischemic stroke that correlated with severity of neurological impairment and clinical outcome.Using single-cell sequencing,we identified Neuregulin-1-positive macrophages among peripheral blood mononuclear cells that produced Neuregulin-1 post-ischemia.In addition,Neuregulin-1 promoted repair of the infarcted area,alleviating neuronal and myelin damage and improving overall behavioral recovery in mice.We found that Neuregulin-1 may exert these neuroprotective effects by promoting angiogenesis in the infarct area,and that this effect is mediated by Akt/mTOR/VEGF-dependent signaling.Our findings suggest that peripheral macrophages are a source of Neuregulin-1 post-stroke.Neuregulin-1 exerts its neuroprotective effects by promoting angiogenesis via Akt/mTOR/VEGF-dependent signaling,showing promising clinical translation potential.
基金supported by the Natural Science Foundation of Shaanxi Province(2023-JC-QN-0728)the China Postdoctoral Science Foundation(2021M693942)。
文摘Manned aerial vehicle-unmanned aerial vehicle(MAV-UAV)combat organization is a MAV-UAV combat collective formed from the perspective of organization design theory and methodology,and the generation of force formation plan is a key step in the organizational planning.Based on the description of the problem and the definition of organizational elements,the matching model of platform-target attack wave is constructed to minimize the redundancy of command and decision-making capability,resource capability and the number of platforms used.Based on the non-dominated sorting genetic algorithmⅢ(NSGA-Ⅲ)framework,which includes encoding/decoding method and constraint handling method,the generation model of organizational force formation plan is solved,and the effectiveness and superiority of the algorithm are verified by simulation experiments.
基金National Natural Science Foundation of China,Grant/Award Number:32302826 and 32372961Jilin Provincial Special Project for Health Research Talents,Grant/Award Number:2020SCZ40China Postdoctoral Science Foundation,Grant/Award Number:2023M740623。
文摘Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth research on related molecular mechanisms is of great significance.Methods:Postpartum mice were anesthetized with pentobarbital and administered lipopolysaccharide to develop the mouse mastitis model.Proteomic analysis was per-formed to compare protein expression in mitochondria-associated endoplasmic retic-ulum membranes(MAM)from two mouse mammary gland groups.Western blot was used to detect the expression of MAM-related proteins in mitochondria.AlphaFold3 was used to predict the molecular structures of phosphofurin acidic cluster sorting protein 2(PACS2)and mitofusin 2(MFN2)and their interaction levels.The MFN2-PACS2 interaction was investigated using co-immunoprecipitation and small interfer-ing RNA.Results:The results showed that the inflammation level in the mammary gland tissue of mice with mastitis significantly increased,the total antioxidant capacity decreased,and the expression of MAM-related proteins MFN2 and PACS2 was significantly downregulated.In cell experiments,overexpression of MFN2 can inhibit inflamma-tion and oxidative stress responses,and promote the interaction between MFN2 and PACS2 to affect the formation of MAMs.Conclusion:In summary,this study suggests that mastitis can alter the expression of MAM-related proteins in mouse breast tissue.The interaction between MFN2 and PACS2 regulates the formation of MAMs.Overexpression of MFN2 can promote the formation of MAMs and inhibit inflammation and oxidative stress response in mam-mary epithelial cells.Our results provided a new theoretical basis and potential thera-peutic targets for the prevention and treatment of mastitis.
基金National Natural Science Foundation of China,42103008,Hongming Cai。
文摘The majority(up to 90%)of riverine materials is transported from the continent to the ocean mainly in flood events.It is thus crucial to characterize the geochemistry of elements and their flux in river system in order to better constrain their global biogeochemical cycling and impact on the oceanic ecosystem.However,the geochemical behavior including the distribution,migration and partitioning of typical metal elements amongst diff erent phases,during hydrodynamic flood event remains still to be well explored.Here,we investigated the geochemical behaviors of typical metal elements in dissolved phase and suspended particulate matter collected from a single flood event in the natural Chishui River,Southwest China.The results showed clearly that the geochemistry of metal elements was largely controlled by the hydrodynamic eff ect,of which the diff erent flowrates introduce a natural sorting of diff erent mineral particles transported at diff erent flood stages,depending on their shape,size and density.The maximum concentrations of alkaline and alkaline earth metals(Li,Mg,K,Rb and Sr)in SPM appeared before the flood peak,which was largely controlled by aluminosilicate minerals.However,transition metals(Cr,Mn,Fe,Ni and Cu)showed their abundance peaks lagging behind the flowrate summit,as a result of the late arrival of coarse particles or heavy minerals,evidenced by the mineralogical phase analysis.In addition,the distribution coe fficient(K_(d))between particulate and dissolved loads were lower and stable for soluble alkali/alkaline earth metals which could be aff ected by pH,while higher and fluctuant for transition metals that were largely influenced by SPM content.Overall,the present study reveals clear eff ects of hydrodynamic sorting on the geochemistry of metal elements during the flood event of the natural Chishui River,which should be taken into account when characterizing the riverine flux and their impact on marine ecosystem.
基金research was funded bymultiple sources,including the Guangxi Natural Science Foundation of Youth Fund(2020GXNSFBA159004)the National Natural Science Foundation Project of China(51779204)the Guilin University of Technology High-Level Talent Research Startup Project(GUTQDJJ2018069).
文摘The particle size distribution plays a crucial role in the transportation and deposition of eroded sediments.Gaining insights into the related sorting mechanism can significantly enhance our understanding of such processes.In this study,sand-covered slopes were examined.A controlled indoor rainfall simulation was conducted on loess slopes with a 12°incline and a rainfall intensity of 1.5 mm/min.These slopes were then covered with sand layers of varying thicknesses—0.5,1.0,and 1.5 cm—to observe their effects.The findings have revealed that as the thickness of the sand cover increases,the content of sediment particles smaller than 0.054 mm decreases.In contrast,the content of particles larger than 0.054 mm increases after the sixth minute of runoff.The eroded sediment was predominantly composed of silt.During the inter-rill erosion stage,runoff primarily transported particles larger than 0.054 mm.However,in the subsequent rill erosion and combined rill and inter-rill erosion stages,the runoff predominantly carried finer particles,smaller than 0.054 mm.Moreover,the presence of the sand layer significantly influenced the size and form of the eroded sediment particles.Initially,during the first 10 min of runoff,the eroded particles were predominantly larger than 0.054 mm.After this period,however,the particle size shifted,with the majority of particles being smaller than 0.054 mm.This study highlights the intricate relationship between sediment sorting,the thickness of sand covers,and the dynamics of sediment transport under rainfall-induced erosion.
基金supported by the National Natural Science Foundation of China(No.32471608)the Open Project of Key Laboratory of Environmental Biotechnology,CAS(No.kf2020002)Yunnan Collaborative Innovation Center for Plateau Lake Ecology and Environmental Health.
文摘Elucidating the mechanisms underlying community assembly remains a central question in community ecology,especially in aquatic ecosystems disrupted by human activities.Understanding the causes and consequences of community responses to changing environment is essential for revealing the ecological effects of anthropogenic disturbances and proposing practical strategies for ecological restoration.While stochastic dispersal and species sorting are known to influence local biological communities,most studies have focused on horizontal dispersal,often neglecting the vertical exchange of organisms between planktonic and sedimentary communities when studying stochastic dispersal.We used a highly disturbed urban river in Beijing as a model system to investigate the relative roles of stochastic dispersal versus species sorting driven by local pollution,as well as two components of stochastic dispersal,vertical exchange and horizontal dispersal,in structuring local bacterial communities.Our integrated analyses of planktonic and sedimentary bacterial communities revealed that,despite different spatial patterns along the river,both types of bacterial communities were primarily shaped by stochastic dispersal processes rather than species sorting influenced by the environmental gradient.Notably,in addition to the effect of horizontal dispersal along the river,the vertical exchange between planktonic and sedimentary bacterial communities significantly contributed to the formation of local communities.These findings suggest that both vertical exchange and horizontal dispersal should be considered when assessing the role of stochastic dispersal in shaping local community structure in microbial communities.
基金Supported by Direktorat Riset dan Pengembangan(Directorate of Research and Development)Universitas Indonesia(NKB-690/UN2.RST/HKP.05.00/2022).
文摘The need to transport goods across countries and islands has resulted in a high demand for commercial vessels.Owing to such trends,shipyards must efficiently produce ships to reduce production costs.Layout and material flow are among the crucial aspects determining the efficiency of the production at a shipyard.This paper presents the initial design optimization of a shipyard layout using Nondominated Sorting Algorithm-Ⅱ(NSGA-Ⅱ)to find the optimal configuration of workstations in a shipyard layout.The proposed method focuses on simultaneously minimizing two material handling costs,namely work-based material handling and duration-based material handling.NSGA-Ⅱ determines the order of workstations in the shipyard layout.The semiflexible bay structure is then used in the workstation placement process from the sequence formed in NSGA-Ⅱ into a complete design.Considering that this study is a case of multiobjective optimization,the performance for both objectives at each iteration is presented in a 3D graph.Results indicate that after 500 iterations,the optimal configuration yields a work-based MHC of 163670.0 WBM-units and a duration-based MHC of 34750 DBM-units.Starting from a random solution,the efficiency of NSGA-Ⅱ demonstrates significant improvements,achieving a 50.19%reduction in work-based MHC and a 48.58%reduction in duration-based MHC.
基金supported by the Guizhou Province High-level Innovative Talent Project(Qiankehe Platform Talent-GCC[2022]027-1)the National Key Research and Development Program of China(2019YFA0904800).
文摘Soft rot is a destructive disease that inflicts significant losses on agricultural production and the economy post-harvest.Biocontrol strategies based on antagonistic microorganisms have a broad application prospect to fight against plant pathogens.This study utilized fluorescence-activated droplet sorting(FADS)technology as an alternative to traditional plate culture methods to isolate microorganisms with antagonistic activity against the soft rot pathogen Erwinia carotovora Ecc15.Initially,the culture performance of the FADS platform was evaluated by analyzing bacterial diversity in droplet culture samples and agar plate culture samples,our data showed that droplet culture exhibited higher species richness and diversity than plate culture,and more than 95%of the operational taxonomic units(OTUs)in the droplet samples belonged to the rare biosphere.Additionally,we developed a green fluorescent protein(GFP)-Ecc15-based FADS screening system,which achieved an enrichment ratio of up to 148.Using this system,we successfully screened 32 antagonistic bacteria from rhizosphere soil sample of healthy konjac plants,and some may be novel microbial resources,including the genera Lelliottia,Buttiauxella and Leclercia.Notably,strain D-62 exhibited the strongest antibacterial ability against Ecc15,with an inhibition zone diameter of(20.86±1.56)mm.In vivo experiments conducted on the corms of Amorphophallus konjac demonstrated that strain D-62 could effectively reduce the infection ability of Ecc15 to the corms,indicating that strain D-62 has the potential to be developed as a biocontrol agent.Our findings suggested that the FADS screening system showed a screening efficiency approximately 3×10^(3)times higher than plate screening system,while significantly reducing costs of infrastructure,labor and consumables,it provides theoretical guidance for the screening of other plant pathogen biocontrol bacteria.
文摘本文聚焦于基于多目标跟踪的课堂人数自动统计这一主题,提出将YOLOv8和SORT (Simple Online and Realtime Tracking)算法相结合的方法。其中,YOLOv8作为先进的单阶段目标检测算法,能迅速识别图像或视频帧中的目标并输出关键信息;SORT算法借助卡尔曼滤波预测目标位置变化。研究通过运用特定的软硬件配置开展实验,对模型进行训练与验证。该方法旨在实现课堂人数的精准自动统计,满足课堂管理和动态监控的需求,为教师及管理人员提供有力工具。其不仅在算法应用上具有创新性,而且在教育管理实践中具有重要的实用价值,有望对智慧教育领域的课堂管理产生积极深远的影响。This paper focuses on the theme of automatic counting of the number of people in the classroom based on multi-object tracking and proposes a method that combines the YOLOv8 and SORT algorithms. Among them, YOLOv8, as an advanced single-stage target detection algorithm, can quickly identify targets in an image or video frame and output key information. The SORT algorithm predicts the position change of the target with the help of Kalman filtering. The research conducts experiment by using specific software and hardware configurations to train and validate the model. This method aims to achieve accurate automatic counting of the number of people in the classroom, meet the needs of classroom management and dynamic monitoring, and provide a powerful tool for teachers and administrators. It is not only innovative in algorithm application, but also has important practical value in educational management practice, and is expected to have a positive and far-reaching impact on classroom management in the field of smart education.
文摘煤矿井下安全生产是保障矿工生命安全和能源稳定供应的核心环节,但传统监控方法在检测实时性和准确性方面存在明显不足。针对复杂井下环境中人员入侵识别精度低的问题,研究提出一种基于改进You Only Look Once version 5(YOLOv5)和深度简单在线实时跟踪算法的电子围栏入侵检测技术。研究通过嵌入注意力机制增强模型对关键特征的感知能力,并利用扩展卡尔曼滤波与匈牙利算法提升跟踪稳定性。实验结果表明,改进后的模型的识别率最高,且随迭代次数的增加其识别率始终在90%以上。该技术在识别性能上,平均精度均值指标为92.5%,每秒帧数提升至116.7,训练时长缩短至8.5 h,漏检率显著降低。研究表明,该方法在光照不均、煤尘干扰等复杂场景下具备更高的检测精度与实时性。该技术的提出可为煤矿井下智能化安全管理提供有效技术支撑,从而提高煤矿安全生产水平,杜绝生产事故出现。
