Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth...Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth research on related molecular mechanisms is of great significance.Methods:Postpartum mice were anesthetized with pentobarbital and administered lipopolysaccharide to develop the mouse mastitis model.Proteomic analysis was per-formed to compare protein expression in mitochondria-associated endoplasmic retic-ulum membranes(MAM)from two mouse mammary gland groups.Western blot was used to detect the expression of MAM-related proteins in mitochondria.AlphaFold3 was used to predict the molecular structures of phosphofurin acidic cluster sorting protein 2(PACS2)and mitofusin 2(MFN2)and their interaction levels.The MFN2-PACS2 interaction was investigated using co-immunoprecipitation and small interfer-ing RNA.Results:The results showed that the inflammation level in the mammary gland tissue of mice with mastitis significantly increased,the total antioxidant capacity decreased,and the expression of MAM-related proteins MFN2 and PACS2 was significantly downregulated.In cell experiments,overexpression of MFN2 can inhibit inflamma-tion and oxidative stress responses,and promote the interaction between MFN2 and PACS2 to affect the formation of MAMs.Conclusion:In summary,this study suggests that mastitis can alter the expression of MAM-related proteins in mouse breast tissue.The interaction between MFN2 and PACS2 regulates the formation of MAMs.Overexpression of MFN2 can promote the formation of MAMs and inhibit inflammation and oxidative stress response in mam-mary epithelial cells.Our results provided a new theoretical basis and potential thera-peutic targets for the prevention and treatment of mastitis.展开更多
The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Ti...The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Time-sensitive networking(TSN)is proposed by IEEE 802.1TSN working group.In order to achieve low latency,Cyclic queuing and forwarding(CQF)mechanism is introduced to schedule Timetriggered(TT)flows.In this paper,we construct a TSN model based on CQF and formulate the flow scheduling problem as an optimization problem aimed at maximizing the success rate of flow scheduling.The problem is tackled by a novel algorithm that makes full use of the characteristics and the relationship between the flows.Firstly,by K-means algorithm,the flows are initially partitioned into subsets based on their correlations.Subsequently,the flows within each subset are sorted by a new special criteria extracted from multiple features of flow.Finally,a flow offset selecting method based on load balance is used for resource mapping,so as to complete the process of flow scheduling.Experimental results demonstrate that the proposed algorithm exhibits significant advantages in terms of scheduling success rate and time efficiency.展开更多
The somatotopic representation of specific body parts is a well-established spatial organizational principle in the primary somatosensory and motor cortices.
在工业化建造和碳减排的背景下,预制装配化得到了极大关注。以装配式铁路桥梁为对象,首先,考虑4种典型构件类型(箱梁、桥面板、桥墩和盖梁),核算其预制与现浇两种方式在物化阶段的立方单项碳排放和建造成本,并构建以构件预制率为决策变...在工业化建造和碳减排的背景下,预制装配化得到了极大关注。以装配式铁路桥梁为对象,首先,考虑4种典型构件类型(箱梁、桥面板、桥墩和盖梁),核算其预制与现浇两种方式在物化阶段的立方单项碳排放和建造成本,并构建以构件预制率为决策变量的碳排放-成本双目标优化模型,系统优化预制构件的选择与组合;其次,采用非支配排序的鲸鱼优化算法(Non-Dominated Sorting Whale Optimization Algorithm,NSWOA)对构件组合方案进行求解,获得Pareto均衡解集;再次,通过博弈论思想融合主客观权重,并采用优劣解距离法(Technique for Order Preference by Similarity to an Ideal Solution,TOPSIS)对Pareto均衡解集进行科学决策,得到最佳构件组合方案;最后,以某西部装配式铁路桥梁为例开展实证分析。结果显示:优化方案较初始方案碳排放减少了361.66 t,成本减少了213.06万元。此外,预制率情景分析显示,不同的桥梁预制率对应不同的最佳构件组合,且碳排放与成本随预制率变化呈现不同趋势。研究成果可为装配式铁路桥梁低碳施工提供有效的优化策略。展开更多
鱼群多目标准确计数是水生态智能监测和集约化养殖产业中的重要环节,对水域生态环境智能保护和水产养殖现代化具有重要作用。现有鱼群多目标准确追踪和计数方法主要适用于鱼群外观清晰、游速缓慢和方向稳定等较理想的情况,难以有效适用...鱼群多目标准确计数是水生态智能监测和集约化养殖产业中的重要环节,对水域生态环境智能保护和水产养殖现代化具有重要作用。现有鱼群多目标准确追踪和计数方法主要适用于鱼群外观清晰、游速缓慢和方向稳定等较理想的情况,难以有效适用于现实情况下存在的鱼群互相遮挡、游动迅速和方向多变等复杂情况。为此,结合轻量化目标检测模型YOLOv5n,提出基于水平相似度匹配机制的鱼群追踪与计数方法。将鱼群计数问题视为多目标检测与追踪问题,设计水平相似度匹配机制,并对SORT(Simple Online and Realtime Tracking)算法进行优化。通过高速水流中鱼群个体在帧与帧之间的位置关系对检测框中心点的水平距离进行限制,以有效解决SORT算法存在的目标匹配混乱问题,显著提高追踪效果。实验结果表明,所提方法在鱼群多目标追踪数据集上的性能显著优于现有追踪方法,对目标遮挡、方向变化等情况目标追踪性能提升显著,并且该方法结构简单,易于实际应用。展开更多
基金National Natural Science Foundation of China,Grant/Award Number:32302826 and 32372961Jilin Provincial Special Project for Health Research Talents,Grant/Award Number:2020SCZ40China Postdoctoral Science Foundation,Grant/Award Number:2023M740623。
文摘Background:Mastitis seriously affects the mammary health of humans and animals.Studies have found that inflammation and oxidative stress play key roles in the occur-rence and development of mastitis.Therefore,in-depth research on related molecular mechanisms is of great significance.Methods:Postpartum mice were anesthetized with pentobarbital and administered lipopolysaccharide to develop the mouse mastitis model.Proteomic analysis was per-formed to compare protein expression in mitochondria-associated endoplasmic retic-ulum membranes(MAM)from two mouse mammary gland groups.Western blot was used to detect the expression of MAM-related proteins in mitochondria.AlphaFold3 was used to predict the molecular structures of phosphofurin acidic cluster sorting protein 2(PACS2)and mitofusin 2(MFN2)and their interaction levels.The MFN2-PACS2 interaction was investigated using co-immunoprecipitation and small interfer-ing RNA.Results:The results showed that the inflammation level in the mammary gland tissue of mice with mastitis significantly increased,the total antioxidant capacity decreased,and the expression of MAM-related proteins MFN2 and PACS2 was significantly downregulated.In cell experiments,overexpression of MFN2 can inhibit inflamma-tion and oxidative stress responses,and promote the interaction between MFN2 and PACS2 to affect the formation of MAMs.Conclusion:In summary,this study suggests that mastitis can alter the expression of MAM-related proteins in mouse breast tissue.The interaction between MFN2 and PACS2 regulates the formation of MAMs.Overexpression of MFN2 can promote the formation of MAMs and inhibit inflammation and oxidative stress response in mam-mary epithelial cells.Our results provided a new theoretical basis and potential thera-peutic targets for the prevention and treatment of mastitis.
基金supported by Science and Technology Project of State Grid Corporation Headquarters under Grant 5108-202218280A-2-170-XG(Development and Application of Power Time-Sensitive Network Switching Chip。
文摘The progress of modern industry has given rise to great requirements for network transmission latency and reliability in domains such as smart grid and intelligent driving.To address these challenges,the concept of Time-sensitive networking(TSN)is proposed by IEEE 802.1TSN working group.In order to achieve low latency,Cyclic queuing and forwarding(CQF)mechanism is introduced to schedule Timetriggered(TT)flows.In this paper,we construct a TSN model based on CQF and formulate the flow scheduling problem as an optimization problem aimed at maximizing the success rate of flow scheduling.The problem is tackled by a novel algorithm that makes full use of the characteristics and the relationship between the flows.Firstly,by K-means algorithm,the flows are initially partitioned into subsets based on their correlations.Subsequently,the flows within each subset are sorted by a new special criteria extracted from multiple features of flow.Finally,a flow offset selecting method based on load balance is used for resource mapping,so as to complete the process of flow scheduling.Experimental results demonstrate that the proposed algorithm exhibits significant advantages in terms of scheduling success rate and time efficiency.
文摘The somatotopic representation of specific body parts is a well-established spatial organizational principle in the primary somatosensory and motor cortices.
文摘在工业化建造和碳减排的背景下,预制装配化得到了极大关注。以装配式铁路桥梁为对象,首先,考虑4种典型构件类型(箱梁、桥面板、桥墩和盖梁),核算其预制与现浇两种方式在物化阶段的立方单项碳排放和建造成本,并构建以构件预制率为决策变量的碳排放-成本双目标优化模型,系统优化预制构件的选择与组合;其次,采用非支配排序的鲸鱼优化算法(Non-Dominated Sorting Whale Optimization Algorithm,NSWOA)对构件组合方案进行求解,获得Pareto均衡解集;再次,通过博弈论思想融合主客观权重,并采用优劣解距离法(Technique for Order Preference by Similarity to an Ideal Solution,TOPSIS)对Pareto均衡解集进行科学决策,得到最佳构件组合方案;最后,以某西部装配式铁路桥梁为例开展实证分析。结果显示:优化方案较初始方案碳排放减少了361.66 t,成本减少了213.06万元。此外,预制率情景分析显示,不同的桥梁预制率对应不同的最佳构件组合,且碳排放与成本随预制率变化呈现不同趋势。研究成果可为装配式铁路桥梁低碳施工提供有效的优化策略。
文摘鱼群多目标准确计数是水生态智能监测和集约化养殖产业中的重要环节,对水域生态环境智能保护和水产养殖现代化具有重要作用。现有鱼群多目标准确追踪和计数方法主要适用于鱼群外观清晰、游速缓慢和方向稳定等较理想的情况,难以有效适用于现实情况下存在的鱼群互相遮挡、游动迅速和方向多变等复杂情况。为此,结合轻量化目标检测模型YOLOv5n,提出基于水平相似度匹配机制的鱼群追踪与计数方法。将鱼群计数问题视为多目标检测与追踪问题,设计水平相似度匹配机制,并对SORT(Simple Online and Realtime Tracking)算法进行优化。通过高速水流中鱼群个体在帧与帧之间的位置关系对检测框中心点的水平距离进行限制,以有效解决SORT算法存在的目标匹配混乱问题,显著提高追踪效果。实验结果表明,所提方法在鱼群多目标追踪数据集上的性能显著优于现有追踪方法,对目标遮挡、方向变化等情况目标追踪性能提升显著,并且该方法结构简单,易于实际应用。
