WRKY transcription factors are widely distributed in higher plants and play important roles in many biological processes,including stress resistance.The recently published genome sequence of yellowhorn,an oil tree wit...WRKY transcription factors are widely distributed in higher plants and play important roles in many biological processes,including stress resistance.The recently published genome sequence of yellowhorn,an oil tree with robust resistance to cold,drought,heat,salt and alkali,provides an excellent opportunity to identify and characterize the entire yellowhorn WRKY protein family and a basis for the study of abiotic stress resistance of WRKY gene family in forest species.In the present comprehensive analysis of WRKY transcription factors in yellowhorn,65 WRKY genes were identified and defined based on their location on the chromosome.According to their structure and phylogenetic relationships,XsWRKY genes clustered into WRKY groupsⅠ-Ⅲ.Segmental duplication events played a significant role in the expansion of WRKY gene family.Furthermore,transcriptomic data and real-time quantitative PCR analysis showed that expression of XsWRKY genes responding to salt and drought stresses and a hormone treatment.We also determined structures of the encoded proteins,c is-elements of the promoter region,and expression patterns.These results provide a foundation for the study of the biological function of WRKY transcription factors in yellowhorn.展开更多
Yellowhorn （Xanthoceras sorbifolium Bunge） is a drought-tolerant tree in the family Sapindaceae that is native to northeastern China. Its remarkably oil-rich seeds are a reliable biodiesel source. An inter simple se...Yellowhorn （Xanthoceras sorbifolium Bunge） is a drought-tolerant tree in the family Sapindaceae that is native to northeastern China. Its remarkably oil-rich seeds are a reliable biodiesel source. An inter simple sequence repeat （ISSR） analysis showed genetic variation among four artificial populations in China： two in Inner Mongolia （IM）, one in Liaoning （LN）, and one in Shandong （SD）. The average percentage of polymorphic loci was 81.25 % for these four populations. The Forest Farm in SD showed the highest number of effective alleles （Ne）, Shannon index （I）, and expected heterozygosity （He）, i.e., 1.598, 0.470, and 0.325, respectively, but the lowest number of alleles （Na） as 1.600. Based on an analysis of molecular variance, 23 % of the total genetic variation was found among populations, and 77 % within populations. A principal coordinate analysis revealed two groups （Group 1： Lindonglinchang, Jianping Agriculture Research Station and Forest Farm; Group 2： Jinjilinchang）. Understanding the genetic diversity among artificial yellowhorn populations in China, detected using ISSRs, will be useful for yellowhorn conservation and improvement. Additional arti-ficial and natural populations need to be included in the future for a country-wide perspective.展开更多
Cutting breeding is an important method of asexual reproduction of Xanthoceras sorbifolium Bunge.At present,it has been found that the factors influencing the cuttings of X.sorbifolium mainly include cutting material ...Cutting breeding is an important method of asexual reproduction of Xanthoceras sorbifolium Bunge.At present,it has been found that the factors influencing the cuttings of X.sorbifolium mainly include cutting material type,substrate type,cutting season,cutting material length and thickness,mother tree age,cutting material pretreatment method,hormone species concentration and soaking time.Besides,different regions,control conditions,and germplasm types have different cutting rooting rates.This paper introduced some of the problems in the cuttings of X.sorbifolium,and came up with some recommendations to provide a reference for the future research and technical promotion of X.sorbifolium cuttings.展开更多
该文以文冠果种仁油为芯材,以阿拉伯胶-麦芽糊精(acacia senegal-maltodextrin,AS-MD)或大豆分离蛋白-麦芽糊精(soy protein isolate-maltodextrin,SPI-MD)为壁材,制备了5组不同壁材构成的文冠果油微胶囊粉末。通过对文冠果油微胶囊的...该文以文冠果种仁油为芯材,以阿拉伯胶-麦芽糊精(acacia senegal-maltodextrin,AS-MD)或大豆分离蛋白-麦芽糊精(soy protein isolate-maltodextrin,SPI-MD)为壁材,制备了5组不同壁材构成的文冠果油微胶囊粉末。通过对文冠果油微胶囊的包埋率、理化指标、微观形态、体相结构和热稳定性进行比较研究,确定最优壁材配比。结果表明,由质量比为3∶2的SPI-MD壁材组成制备的微胶囊包埋率高达77.09%,湿润性和溶解性等理化性质均优于其他组;该组微胶囊的质量保留率和结晶度最高,氧化稳定性明显提升,表面结构呈完整球形、结构致密。总之,SPI和MD作为壁材对文冠果油具有较好的包埋和保护作用,最优壁材配比为3∶2。该研究结果为优化文冠果油微胶囊的制备工艺提供了理论依据。展开更多
基金supported by the Fundamental Research Funds for the Central Universities(2572017DA03)Development and Identification of Molecular Markers for Fine Strains of Xanthoceras sorbifolia(MOMA-2019-ZENITHGENE)。
文摘WRKY transcription factors are widely distributed in higher plants and play important roles in many biological processes,including stress resistance.The recently published genome sequence of yellowhorn,an oil tree with robust resistance to cold,drought,heat,salt and alkali,provides an excellent opportunity to identify and characterize the entire yellowhorn WRKY protein family and a basis for the study of abiotic stress resistance of WRKY gene family in forest species.In the present comprehensive analysis of WRKY transcription factors in yellowhorn,65 WRKY genes were identified and defined based on their location on the chromosome.According to their structure and phylogenetic relationships,XsWRKY genes clustered into WRKY groupsⅠ-Ⅲ.Segmental duplication events played a significant role in the expansion of WRKY gene family.Furthermore,transcriptomic data and real-time quantitative PCR analysis showed that expression of XsWRKY genes responding to salt and drought stresses and a hormone treatment.We also determined structures of the encoded proteins,c is-elements of the promoter region,and expression patterns.These results provide a foundation for the study of the biological function of WRKY transcription factors in yellowhorn.
基金supported by the Cooperative Research Program for Agriculture Science & Technology Development(Project no.PJ01117902) of the National Institute of Horticultural and Herbal Science,RDA,Republic of Koreathe Ministry of Education,People’s Republic of China(Program No.MS2012DBLY017 for Foreign Distinguished Scientists of the Ministry of Education)the Research Foundation of Kangwon National University,Republic of Korea
文摘Yellowhorn （Xanthoceras sorbifolium Bunge） is a drought-tolerant tree in the family Sapindaceae that is native to northeastern China. Its remarkably oil-rich seeds are a reliable biodiesel source. An inter simple sequence repeat （ISSR） analysis showed genetic variation among four artificial populations in China： two in Inner Mongolia （IM）, one in Liaoning （LN）, and one in Shandong （SD）. The average percentage of polymorphic loci was 81.25 % for these four populations. The Forest Farm in SD showed the highest number of effective alleles （Ne）, Shannon index （I）, and expected heterozygosity （He）, i.e., 1.598, 0.470, and 0.325, respectively, but the lowest number of alleles （Na） as 1.600. Based on an analysis of molecular variance, 23 % of the total genetic variation was found among populations, and 77 % within populations. A principal coordinate analysis revealed two groups （Group 1： Lindonglinchang, Jianping Agriculture Research Station and Forest Farm; Group 2： Jinjilinchang）. Understanding the genetic diversity among artificial yellowhorn populations in China, detected using ISSRs, will be useful for yellowhorn conservation and improvement. Additional arti-ficial and natural populations need to be included in the future for a country-wide perspective.
基金Biosafety and Genetic Resources Management Project of China National Forestry and Grassland Administration(KJZXSA202033).
文摘Cutting breeding is an important method of asexual reproduction of Xanthoceras sorbifolium Bunge.At present,it has been found that the factors influencing the cuttings of X.sorbifolium mainly include cutting material type,substrate type,cutting season,cutting material length and thickness,mother tree age,cutting material pretreatment method,hormone species concentration and soaking time.Besides,different regions,control conditions,and germplasm types have different cutting rooting rates.This paper introduced some of the problems in the cuttings of X.sorbifolium,and came up with some recommendations to provide a reference for the future research and technical promotion of X.sorbifolium cuttings.
文摘该文以文冠果种仁油为芯材,以阿拉伯胶-麦芽糊精(acacia senegal-maltodextrin,AS-MD)或大豆分离蛋白-麦芽糊精(soy protein isolate-maltodextrin,SPI-MD)为壁材,制备了5组不同壁材构成的文冠果油微胶囊粉末。通过对文冠果油微胶囊的包埋率、理化指标、微观形态、体相结构和热稳定性进行比较研究,确定最优壁材配比。结果表明,由质量比为3∶2的SPI-MD壁材组成制备的微胶囊包埋率高达77.09%,湿润性和溶解性等理化性质均优于其他组;该组微胶囊的质量保留率和结晶度最高,氧化稳定性明显提升,表面结构呈完整球形、结构致密。总之,SPI和MD作为壁材对文冠果油具有较好的包埋和保护作用,最优壁材配比为3∶2。该研究结果为优化文冠果油微胶囊的制备工艺提供了理论依据。
